thank you very much sir, very informative.

Very useful sir, thanks.

Thank you so much for making this fantastic video. I have watched 10 videos by after this video all doubts become clear. if it is possible, please make some video on shotgun whole genome sequencing of bacteria.

Extremely good explanation

@DweipayanG

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Thank you !!

Thanks a lot!

thanks, its useful video

how did you get the chromatogram in bioedit. i am only getting the sequence in bioedit and did not know how you opened both the bioedit and chromatogram to delete uncorrect nucleotides.

Sir forward primer always binds with anti sense strand and reverse primer binds with sense strand.

Thank you so much

sir the lesson is useful. can u also make a video on "how to align our trimmed consensus 16s rRNA sequence with BLAST hit sequences, including some whole genome sequences hits in MEGA software". Thank you

Hi, what do you do when there are gaps between the sequences after aligning them?

The quary length in this example is 403 , i used the same step and i get 665, please i want to ask is this query length acceptable to be be submitted to NCBI to register the bacteria ?

How about for 4peaks?

From where we can download forward and reverse sequences?

please give the website link of bioedit software, bcz there is more than 3-4.

Hello sir, does forward mean 5 prime to 3 prime?

@DweipayanG

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Forward mean , the amplicon that u get by extension of forward primer

Please guide me, I am stuck as my consensus sequence is coming in this form. what to do? Example >Consensus -------------------------------------------CTGCCAGTAAGACAGGGATAACGCCCGGAAAC-GAAGCAAATAACGCATA--AACCACTCCGCCAGGGGAG-ACGATCGAAAGACGGAAACGCAAAGCAATT---GACGGGCCCCCGCA-CAAGAGCTAGAGCATGAGGTA-AAGGCGAACCAACGCAAAGAACCATACCAGACCTG-GACA-TCATCGGACA-ACT----CT-AGACCAAAAAACAAGGCTCAACCC-GGAGGGTAAGCGGAAACTCGCCAGCCCGAAA-CACCCAGATAATCC-------------------

Sir i have some problems to solving Blast. Can i contact with you through mail or contact number ?