High Performance Liquid Chromatography (HPLC) - Operations by Dr. Sejal P. Gandhi

This video is a virtual tour to Shimadzu HPLC system available at Central Instrumentation Facility of Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune. Viewers will be able to understand Standard Operating Procedure of HPLC system. It involves all practical aspects in operation of HPLC by demonstration practical of Assay of Paracetamol by RP-HPLC method.

Пікірлер: 71

  • @azhar17kkk
    @azhar17kkk Жыл бұрын

    I work on hplc and by far this is the best hplc demonstration I've found. Thanks ma'am

  • @varungowdahb9221
    @varungowdahb92213 жыл бұрын

    Excellent teaching with instrumentation love from Karnataka ❤️

  • @yaridosti-4867
    @yaridosti-48672 жыл бұрын

    Thank you for the details explanation mam, I look forward to watch more videos like these. Best luck

  • @parulsharma3143
    @parulsharma31433 жыл бұрын

    So much clear cut interpretation on this soft ware..thank u mam

  • @matteof6340
    @matteof6340 Жыл бұрын

    Does the vacuum pump has a trap? This content is absolute gold for the clarity by how it is explained

  • @gaurihire2629
    @gaurihire26296 ай бұрын

    Thank you so much ma'am By watching this video I am much clarified about the instrument 👍

  • @sonalimahaparale4765
    @sonalimahaparale47652 жыл бұрын

    Excellent information about working of HPLC

  • @shashiranjan2123
    @shashiranjan21233 жыл бұрын

    Very much effective teaching method.....I tried to learn and understand the concepts.... thank you so much Ma'am

  • @ahshanalikhan5708

    @ahshanalikhan5708

    Жыл бұрын

    Sir now this is your turn to teach us 😅

  • @pratibhameshram6205
    @pratibhameshram62052 жыл бұрын

    Nice explanation mam, thank you for such important content

  • @hninmaylwin123
    @hninmaylwin1232 жыл бұрын

    Thank you so so much for excellent teaching

  • @tandeldevang6083
    @tandeldevang60832 жыл бұрын

    Nice way to present the HPLC

  • @mohdimranansari5264
    @mohdimranansari52643 жыл бұрын

    Nice demo of paracetamol analysis........Is there any further video related to HPLC?

  • @malathibalu7467
    @malathibalu7467 Жыл бұрын

    very much thank you madam, very good explanation.

  • @From_the_city_of_Queen_Jhansi
    @From_the_city_of_Queen_Jhansi3 жыл бұрын

    Easy to understand in first view, Thank you Mam for the video

  • @suryaindian8457
    @suryaindian84572 жыл бұрын

    Very nice मैम, upload more videos. 👌👌👌👌I am working in pharmacetucal industry (laboratory)

  • @aynomwolda8250
    @aynomwolda82503 жыл бұрын

    Nice explanation , thank you .

  • @sarojkumarraul3165
    @sarojkumarraul3165 Жыл бұрын

    Thank you so much mam 😊 for Amazing teaching 🙏

  • @chemistry9540
    @chemistry95403 жыл бұрын

    it is very nice teaching . can you add auto- sampler injection ?

  • @rajaryanbiotech
    @rajaryanbiotech Жыл бұрын

    First filtration followed by sonication since its role is to remove air traps. If you do first sonication again filtration then environmental air entraps the solution and you will get negative peaks due to air bubbles.

  • @moabiekj8180

    @moabiekj8180

    11 ай бұрын

    I think that the vacuum created by the vacuum pump not only allows suction/filtering, but also catching any air in the solution. But you're right, first filtering and then sonication seems better. Have you noticed that the filter is smaller than the opening of the bottle and some of the solution may have flowed sideways, bypassing the filter?

  • @memories41941
    @memories419412 жыл бұрын

    It's really helpful Mam Thank you

  • @ydb6061
    @ydb60612 жыл бұрын

    Thank u so much mam plz make the videos on UV like as these video.

  • @RAJGAMING5.0
    @RAJGAMING5.0 Жыл бұрын

    Mam excellent teaching ❤

  • @sonajigaikwad6863
    @sonajigaikwad68632 жыл бұрын

    Excellent session

  • @knoweasy
    @knoweasy3 жыл бұрын

    Informative session 👍

  • @ashudada9482
    @ashudada94822 жыл бұрын

    Nice explanation mam thanku so much mam

  • @vaishnavidhimmar1027
    @vaishnavidhimmar10272 жыл бұрын

    Great video.

  • @adhixxsanju
    @adhixxsanju2 жыл бұрын

    What is the standard taken here?? And how did u arrive at the formula. Generally if we have a linear equation of standard samples of different conc we can find out the unknown concentration.

  • @ganeshyeole5902
    @ganeshyeole59023 жыл бұрын

    Nice explanation mam👍

  • @swathinanu665
    @swathinanu6653 жыл бұрын

    Excellent mam 👏👏👏

  • @dr.reshama6427
    @dr.reshama6427Ай бұрын

    very nice explaination

  • @sowmiyashanker150
    @sowmiyashanker1502 жыл бұрын

    Thank you mam

  • @ketanvyas1566
    @ketanvyas15663 жыл бұрын

    Nice info mam✌

  • @ko-fanta9829
    @ko-fanta9829 Жыл бұрын

    Thanks 😊

  • @kalpeshnerkar4062
    @kalpeshnerkar40622 жыл бұрын

    Mam HPLC shimandzu LC CHT 2010 for the purging valve open krte hai ya nahi

  • @MUSMAN-pc6wf
    @MUSMAN-pc6wf3 жыл бұрын

    Good job mam.

  • @rajasaravanan1967
    @rajasaravanan19672 жыл бұрын

    Calibration curve start with 1 MCG 2 MCG ....... 5 MCG. But actual concentration 5 MCG , 10 MCG,...... 50 mcg

  • @rothmanfamilies7679
    @rothmanfamilies76792 жыл бұрын

    can solvent be filtered if there is acetonitrile?

  • @LailaAlly
    @LailaAllyАй бұрын

    Thanks more

  • @mariahassanmariahassan6536
    @mariahassanmariahassan6536 Жыл бұрын

    Excellent Madam

  • @kamleshthakur3871
    @kamleshthakur38712 жыл бұрын

    Nicely explain

  • @pharmasolutionpakistan7668
    @pharmasolutionpakistan76682 жыл бұрын

    Good effort

  • @educationpoint778
    @educationpoint7788 ай бұрын

    appreciated from china

  • @safiahameed1536
    @safiahameed15362 жыл бұрын

    Explain it deeply kindly an Jazakallah

  • @ajithpharmacist6204
    @ajithpharmacist6204 Жыл бұрын

    Excellent teaching mam I'm so happy tqq so much

  • @lakykhatun7119
    @lakykhatun7119 Жыл бұрын

    Mam how to choose mobile for diferent analysis like for vitamin B group and for vitamin E

  • @gulshansinghrajput9403
    @gulshansinghrajput9403 Жыл бұрын

    Supper ❤️

  • @amolgujar211
    @amolgujar211 Жыл бұрын

    Nothing like actual in company

  • @Prince-hv8di

    @Prince-hv8di

    Жыл бұрын

    Instruments use krna difficult hota hai kya bro???

  • @nirajpatil3757
    @nirajpatil37573 жыл бұрын

    👍👍

  • @akashnawkhare1785
    @akashnawkhare1785 Жыл бұрын

    U should sonicate the m.p after filtration, not before that

  • @carlosjust83

    @carlosjust83

    11 ай бұрын

    Can you helpme to operatehaplc?

  • @sanjaysanjaygomtiwal4453

    @sanjaysanjaygomtiwal4453

    6 ай бұрын

    Yess bro !

  • @gutemajebe4365
    @gutemajebe43652 жыл бұрын

    IS THERE ANY EQUIPMENT THAT CAN REPLACE IT?

  • @Safi_01
    @Safi_014 ай бұрын

    How much blood required for HPLC

  • @chitigori7349
    @chitigori73492 жыл бұрын

    sir mujy hplc ka software chahye... sir i need the software of hplc.. ( Hitachi )

  • @qamaruddinast5696
    @qamaruddinast56962 жыл бұрын

    👍

  • @mohammedraheesmuthanikat3258
    @mohammedraheesmuthanikat32582 жыл бұрын

    Is Any vacancies available?

  • @boringperson9779
    @boringperson97792 ай бұрын

    Hamare college me to ye sab sikhaya hi nhi gaya

  • @rachittyagi9436
    @rachittyagi9436 Жыл бұрын

    What is purge

  • @bsunitha118

    @bsunitha118

    Ай бұрын

    Removal of undesirable products

  • @anupamshukla803
    @anupamshukla8032 жыл бұрын

    Maim apka sop or guidelines copy mil skti h

  • @VivekKumar-lj1ft
    @VivekKumar-lj1ft2 жыл бұрын

    Can u make this vedio in hindi language

  • @puspitachal9314
    @puspitachal93142 жыл бұрын

    Calculation part is not clear..

  • @amitamitkumar1261

    @amitamitkumar1261

    2 ай бұрын

    Real

  • @naturevibesbypatil
    @naturevibesbypatil Жыл бұрын

    Are hindi mai explain karana chahiye tha indian hokar english mai explain kiya bakiyoko bhi samaj aana chahiye......

  • @VirendraKumar-ni5ss
    @VirendraKumar-ni5ss2 жыл бұрын

    Your lab is very old model use in instrument

  • @dr.nileshjadhav9569
    @dr.nileshjadhav95692 жыл бұрын

    Go slow ur are talking as if u have rattofy it