High Performance Liquid Chromatography (HPLC) - Operations by Dr. Sejal P. Gandhi
This video is a virtual tour to Shimadzu HPLC system available at Central Instrumentation Facility of Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune. Viewers will be able to understand Standard Operating Procedure of HPLC system. It involves all practical aspects in operation of HPLC by demonstration practical of Assay of Paracetamol by RP-HPLC method.
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I work on hplc and by far this is the best hplc demonstration I've found. Thanks ma'am
Excellent teaching with instrumentation love from Karnataka ❤️
Thank you for the details explanation mam, I look forward to watch more videos like these. Best luck
So much clear cut interpretation on this soft ware..thank u mam
Does the vacuum pump has a trap? This content is absolute gold for the clarity by how it is explained
Thank you so much ma'am By watching this video I am much clarified about the instrument 👍
Excellent information about working of HPLC
Very much effective teaching method.....I tried to learn and understand the concepts.... thank you so much Ma'am
@ahshanalikhan5708
Жыл бұрын
Sir now this is your turn to teach us 😅
Nice explanation mam, thank you for such important content
Thank you so so much for excellent teaching
Nice way to present the HPLC
Nice demo of paracetamol analysis........Is there any further video related to HPLC?
very much thank you madam, very good explanation.
Easy to understand in first view, Thank you Mam for the video
Very nice मैम, upload more videos. 👌👌👌👌I am working in pharmacetucal industry (laboratory)
Nice explanation , thank you .
Thank you so much mam 😊 for Amazing teaching 🙏
it is very nice teaching . can you add auto- sampler injection ?
First filtration followed by sonication since its role is to remove air traps. If you do first sonication again filtration then environmental air entraps the solution and you will get negative peaks due to air bubbles.
@moabiekj8180
11 ай бұрын
I think that the vacuum created by the vacuum pump not only allows suction/filtering, but also catching any air in the solution. But you're right, first filtering and then sonication seems better. Have you noticed that the filter is smaller than the opening of the bottle and some of the solution may have flowed sideways, bypassing the filter?
It's really helpful Mam Thank you
Thank u so much mam plz make the videos on UV like as these video.
Mam excellent teaching ❤
Excellent session
Informative session 👍
Nice explanation mam thanku so much mam
Great video.
What is the standard taken here?? And how did u arrive at the formula. Generally if we have a linear equation of standard samples of different conc we can find out the unknown concentration.
Nice explanation mam👍
Excellent mam 👏👏👏
very nice explaination
Thank you mam
Nice info mam✌
Thanks 😊
Mam HPLC shimandzu LC CHT 2010 for the purging valve open krte hai ya nahi
Good job mam.
Calibration curve start with 1 MCG 2 MCG ....... 5 MCG. But actual concentration 5 MCG , 10 MCG,...... 50 mcg
can solvent be filtered if there is acetonitrile?
Thanks more
Excellent Madam
Nicely explain
Good effort
appreciated from china
Explain it deeply kindly an Jazakallah
Excellent teaching mam I'm so happy tqq so much
Mam how to choose mobile for diferent analysis like for vitamin B group and for vitamin E
Supper ❤️
Nothing like actual in company
@Prince-hv8di
Жыл бұрын
Instruments use krna difficult hota hai kya bro???
👍👍
U should sonicate the m.p after filtration, not before that
@carlosjust83
11 ай бұрын
Can you helpme to operatehaplc?
@sanjaysanjaygomtiwal4453
6 ай бұрын
Yess bro !
IS THERE ANY EQUIPMENT THAT CAN REPLACE IT?
How much blood required for HPLC
sir mujy hplc ka software chahye... sir i need the software of hplc.. ( Hitachi )
👍
Is Any vacancies available?
Hamare college me to ye sab sikhaya hi nhi gaya
What is purge
@bsunitha118
Ай бұрын
Removal of undesirable products
Maim apka sop or guidelines copy mil skti h
Can u make this vedio in hindi language
Calculation part is not clear..
@amitamitkumar1261
2 ай бұрын
Real
Are hindi mai explain karana chahiye tha indian hokar english mai explain kiya bakiyoko bhi samaj aana chahiye......
Your lab is very old model use in instrument
Go slow ur are talking as if u have rattofy it