Gel filtration chromatography in 5 minutes | Size Exclusion Chromatography | Lab Procedure

this video explains the concept of gel filtration chromatography in less that 5 minutes. Size Exclusion Chromatography concept is depicted with animation.

Пікірлер: 59

  • @generendellaquino5944
    @generendellaquino59443 жыл бұрын

    Thank you so much for this! Keep up the good work :)

  • @nikolaspais9029
    @nikolaspais90293 жыл бұрын

    thank you so much i study biochemistry and this video was really helpful. keep up the good work!

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @maham671

    @maham671

    9 ай бұрын

    Me also studying biochemistry

  • @Galactic_Puss
    @Galactic_Puss7 ай бұрын

    Cool stuff man, I deal with both ionic exchange, size exclusion and ultrafiltration at work and you help me understand how the process actually works

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    7 ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @NEBULAFITNESS9885
    @NEBULAFITNESS98859 ай бұрын

    This video was was helpful for me in microbiology , thanks sir 🙂

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    9 ай бұрын

    Please subscribe to my second channel where I upload career advise youtube.com/@letscrackbioexams94?si=Hhovo7iVObYqyZ5l

  • @debarghyapratimgupta9487
    @debarghyapratimgupta94874 жыл бұрын

    Great.. Thank you!

  • @kimpereira4770
    @kimpereira47702 жыл бұрын

    This helped so much! Thank you!

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @sammrayounas2681
    @sammrayounas26813 жыл бұрын

    Suppose if exclusion range is 5 to 600 then the molecules whose size is below 5 they also trapped between the voids then these molecules also eluted with small size molecules then our reqired size protein will not be obtained is this happen or not sir????? Kindly tell me...

  • @patriciajimenez492
    @patriciajimenez4922 жыл бұрын

    can the elution volume be greater than the total volume of the column?

  • @PriyankaPrasad0811
    @PriyankaPrasad08115 ай бұрын

    Bro thank you for this, like genuinely. I'm currently cramming for my first biochem exam and you did in five minutes what my professor couldn't do in two weeks :P

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    5 ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @shahabkhan7604
    @shahabkhan76046 ай бұрын

    Would be nice if you add lil bit description, written form as summary, one liner so we may revise things from that

  • @syedtoufeeqahmad9023
    @syedtoufeeqahmad90232 жыл бұрын

    Thank u sir

  • @avneetkalra1145
    @avneetkalra11452 жыл бұрын

    thank u for making it alot easier

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @abhiruchisah1595
    @abhiruchisah1595 Жыл бұрын

    thank you .You explaine it very nicely .Im from iiser tirupati pursuing majors in biology

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

  • @laiqaaftab8555
    @laiqaaftab8555 Жыл бұрын

    what is wash in workflow

  • @prakashvincent9836
    @prakashvincent98363 жыл бұрын

    Hi your explanation is good. Just a concern is, in the 0.33 time, you tell that there is binding of proteins in gel filtration column which is wrong

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    I think you got me wrong @ .33 sec I was talking about a general workflow of column chromatography and later pointed out that GFC is bit different than the general workflow ,it involves seiving mechanism in contrast to binding

  • @laibanadeem2664
    @laibanadeem2664Ай бұрын

    You explained really well....

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @laibanadeem2664

    @laibanadeem2664

    Ай бұрын

    @@animatedbiologywitharpan ok

  • @udayselva1476
    @udayselva1476 Жыл бұрын

    Good job brother 💓👌👍

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

  • @KokoriKid1
    @KokoriKid110 ай бұрын

    Great video man

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    10 ай бұрын

    Please share my channel link with your friends and help me to reach big audiance

  • @palvoliuto6059
    @palvoliuto60593 жыл бұрын

    thank you for your great videos. could you please answer a question for me. what type of chromatography would you recommend for filtration of a polypeptide fragment? Thank you.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    If the peptide fragment is previously tagged by his tag / HA tag then one can do affinity chromatography, if slight estimation of size is known then one can do gel filtration, if amino acid composition is known and it has charged residues then one can go for ion exchange as well. So it really depends on what we know about the polypeptide.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    Please share my channel link with your friends and help me reach out to big audience

  • @palvoliuto6059

    @palvoliuto6059

    3 жыл бұрын

    @@animatedbiologywitharpan Thanks you so much my friend! All the best!!!

  • @palvoliuto6059

    @palvoliuto6059

    3 жыл бұрын

    @@animatedbiologywitharpan i will and i have subscribed too!!

  • @hccc8782
    @hccc87823 ай бұрын

    very useful

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @awantikatiwari8080
    @awantikatiwari80804 жыл бұрын

    What does that G represents in that beads chart

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    I think it just the name....it dont have any significant meaning....I am not sure

  • @KamleshKumar-io7hl

    @KamleshKumar-io7hl

    3 жыл бұрын

    i think its mean GEL

  • @khadojaraad8274
    @khadojaraad82742 жыл бұрын

    Thank you ,can you put translating in to Arabic?

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    I don't know Arabic my friend

  • @Suraj_gawali
    @Suraj_gawali Жыл бұрын

    I watching your playlist

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    Please support my chabnel such that i can bring more cobtents like this. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution ( starts from 40rs) means a lot for me.

  • @Suraj_gawali

    @Suraj_gawali

    Жыл бұрын

    What is your education.and what perpose of making vidio

  • @Suraj_gawali

    @Suraj_gawali

    Жыл бұрын

    @@animatedbiologywitharpan bro where are you form ? Give me your Mobile no.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    Go to the about section in the channel you would get everything.

  • @PrinessSonali
    @PrinessSonali4 жыл бұрын

    Sir how to score well in sec C?

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    Just by improving basic concepts and reasoning

  • @PrinessSonali

    @PrinessSonali

    4 жыл бұрын

    I'm very weak in chemistry. Can I still score well with certain reliable chapters only!?

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    I think it’s possible

  • @vietthangduong7629
    @vietthangduong76293 жыл бұрын

    god blesses me pass my text. make my teacher see this cmt plz T.T

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    Please share among your friends and help me to reach big audience

  • @ethanferrer1577
    @ethanferrer15773 жыл бұрын

    Redemption

  • @nerocean09

    @nerocean09

    3 жыл бұрын

    yoooo my slime, i know you dont know mandem like that

  • @harmohitkaur9867
    @harmohitkaur98673 жыл бұрын

    yo can you not use the r slur thank you. if you don’t know why it’s offensive even in a scientific context, look it up.

  • @prasadpitagi8787
    @prasadpitagi87873 жыл бұрын

    The explanation and animation are great, but your accent in irritative. At some point of time in this short video it drew my attention away. Speak either in Indian accent or western.

  • @babangapu8189

    @babangapu8189

    3 жыл бұрын

    So is your accent british? Or like your idol sachin tendulkar