Which topics should be next? Please make suggestions here:

@harikabharadwaj98

3 жыл бұрын

DNA fingerprinting

@nbijetadevi1719

3 жыл бұрын

Microarray

@philippphilipp9832

3 жыл бұрын

Bacteriophage typing method

@darshanhegde7717

3 жыл бұрын

Karyotype versus FISH versus microarray versus targeted exome vs clinical exome

@shukuraopeyemi6670

2 жыл бұрын

Qq% 1%q@a1@100

This is the best video about FISH that I saw on KZread!

Extremely awesome and relevant to the preimplantational genetic testing environment. Well done, clearly explained!

Thank u. Nice german accent ;-)

@thetrollpatrol8799

11 ай бұрын

Why the wink

@niels1318

2 ай бұрын

@@thetrollpatrol8799 ;-)

Excellent work, thank you very much for your time and effort.

Thank you totally clear my confusion! and right to the points!

Wonderful easy to understand video! Thanks Henrik!

English is my second language and the way you talk very slow was just amazing for me, because I'm studying about FISH in my university in Brazil and I'm so proud of me to studying in English. By the way I have a test today wish me luck!

Thanks a lot :))) You just saved me 3 hours 🌟🌟 Keep up the good work !!

Nice explanation, You made the concept easy and digestible, thanks

really clear and helpful....solved my problem about why we used small DNA fragments ,thanks a lot

This helped me so much thank you for explaining in such a calm voice and tempo

@Augetie

Жыл бұрын

@Mixkopf 38 it helped me lmao

Kurz und knackig, danke!

very detail and clear. thanks a lot

Very clear n concise... Thanks a lot really

This video is very helpful to understand FISH easily

This video was so helpful, thank you for your effort

It's so helpful and clear video, thank you so much really

Thanks, that was easy to follow and understand.

Really Thank u so much sir for this video ☺️ it helped me a lot to do my seminar ....God bless you 🙏

That was very helpful, thank you

Helped a lot thank you so much☺️

This was soooo helpful,,thank you

Thank you! Really helpful!

So cool! Thank you!

Greatly well explained

Very helpful thank you so much!

This video was real good!

thank you very much for this video!!!!,I understand all

This is great!

Very nice for understanding thank you

Wow man very nicely done It was very helpful for me Thank a lot.

@henrikslab

5 жыл бұрын

anirudh my thank YOU for watching it and the feedback!

An exemple of how to design a probe for a specific gene will be nice

Crazy German scientist over here. Thanks tho, it was very helpful!

very good video.

Thank youu !!

Useful sir 👏

THANK YOU!

Thank you:)

Thanks very much ❤

THANK YOU 👍👍👍

Very helpful! have you done one of CISH?

thank you !!

Never thought this is how fish are made.

very helpful

Thank u sir

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

thank you

Thank you

Thankyou!

THANKS! NEEDED FOR A PAPER

Thanks!

THANKS FOR YOU

it's really interesting, please stay connected :-)

How the deletion site is known . And what confirms that probe is bound like how it gives signal after binding to dna

Thanks. I need to know how to figure out the used probes! For example: we have thousands of syndromes, do i have to make probes for all of them (especially if I have no idea about symptoms)?

👏👏

Does interphasic fish allow us to detect both anuploidie and microdeletions or just anuploidie?

Thank sir explain mutations.types like this.

do you heat the cells/tissue up to 95 C? will the cells/tissue be destroied by such a high temperature?

Cell fixation Adding probe Denaturation Hybridisations

How you can amplify the flourocent probe by PCR? it wouldn't increase the amount of the probe which is tagged

Nice accent man!

👍

You try to give the video more brightness it will be great if you do

After denaturation, why will the target chromosome will hybridized with the probe instead of rebinding to its original strand?

2 Quick questions 1) how can the ligase seal the fluorescent nucleotides. They need to be bound first right? So my question is how do these labelled nucleotide bind? DNA polymerase will just randomly come along? 2) if i do the labelling before I amplify, how will the PCR be able to copy the fluorescent dntp? thank you

@Pearlblack6387

2 ай бұрын

in my opinion, ligase has some recognition site that recognizes the specific sequences and then it binds and causes ligation. DNA polymerase also has some specific sites that recognize first and then do polymerization. is this correct?

Is it correct that a ARRAY CGH is kinda a reversed FISH? U should def do a video about micro array especially the array CGH as it is also used for chromosomal DNA deficts :)

some time it gives false negative results because some time probe are not properly designed and sometime there is change in annealing temperature ? Am I right sir or any other reason of false negative result ?

Fixation of cell et formaldehyde

Gibt es auch ein Video auf deutsch ?

Warm greetings. The short video is very helpful . But how to identify the specific complementary site in a chromosome . Or how do we know to prepare the complementary probe , on what idea this can be produced? If possible kindly send the answers. Thank you. Dr. Rise de leema .

@henrikslab

2 жыл бұрын

Usually you know the sequence of the complementary site since it is a gene you can look up in genome databases! Best Henrik

Thanks. Is it possible to tell which test do I have to do when I'm facing a syndrome?

@henrikslab

Жыл бұрын

I would ask that a medical doctor!

how to make sure that the probe is fully hybridized not the complimentary DNA ? thanks :)

@henrikslab

3 жыл бұрын

First of all: Good question! My answer is based on this paper: www.sciencedirect.com/science/article/pii/S259000721830008X If you make sure that the concentration of the probe is way higher than the concentration of the target dna (e.g. 10000 probes per dna strand) it should be way more likely that one of these probes bind after denaturation compared with the single sister strand of the dna Hope you could understand my explanation!

Can FISH detect mosaicism in an adult?

Fluorescent in situ hybridization

Where is the DNA that the probe originates come from?

@henrikslab

3 жыл бұрын

The DNA probes for FISH can be ordered by companies which will synthesize them. Is that what you mean?

Is Nick translation And FISH hybridization is same

@henrikslab

5 жыл бұрын

That is a good question, but here is the answer: So nick translation is just the process of getting your labelled probe. With this probe you can do whole process of hybridization then (FISHybridization).

I really do appreciate you Dr Igudia youtube,your commitment towards saving human lives is steadfast ,all the knowledge I got from you and,the help with the herbal meds, I was recently tested negative of Hepatitis B infection,I’m so happy thanks doc

So FISH can help in karotyping too?

@henrikslab

2 жыл бұрын

Yes

What is the principal and the concept of the FISH technique ?

@henrikslab

3 жыл бұрын

It is to detect specific sequences on the patients chromosome (which are altered in disease). With FISH we make this sequence of interest visable using a "fluorescent probe".. this labels the sequence of interest

@Meowmeows24

3 жыл бұрын

@@henrikslab u help me , thank you SM😣🤍🤍🤍

Sir I want videos for chromosome walking

Add probe

Denaturation

Did anybody learn this in grade 12? because in lebanon we do .

@sylvia_forest

3 жыл бұрын

Yes I do. I'm learning this as a new chapter.

@jaafar2562

3 жыл бұрын

@@sylvia_forest ah ok thx

you sound soo german :)

Please hindi me bolo

I don't understand... You never explained about the fish. Clickbait

Seek Christ Jesus YHVH.

sprich doch deutsch henrik