Welcome to Science With Tal!
In this clip of the The Neuromuscular Junction as the model of the chemical synapse video, we will cover the experimental basis for the discovery of vesicle release (exocytosis) and the retrieval of the vesicles (endocytosis). The two experiments presented in this video come from John Heuser and Tom Reese. The video will also cover topics such as freeze-fracture electron microscopy.
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Timestamps
0:00 Introduction
0:16 First evidence of vesicles (1956)
1:02 Heuser & Reese exocytosis experiment (1979)
1:22 Freeze fracture electron microscopy & interpretation of results
4:38 Heuser & Reese endocytosis experiment (1973)
4:15 Quantal theory of release & statistical proof
7:30 Conclusion
Resources used
Here is a list of the resources that I’ve used to produce this video. (Author: title resource)
- Dale Purves: Neuroscience (6th edition)
- Eric Kandel: Principles of neural science (6th edition)
- Robertson, J. David: Some Features of the Ultrastructure of Reptilian
Skeletal Muscle
- Heuser, J E et al. : Synaptic vesicle exocytosis captured by quick freezing
and correlated with quantal transmitter release
- Heuser, J E and T S Reese: Evidence for recycling of synaptic vesicle
membrane during transmitter release at the frog neuromuscular junction
- Llinás, R and Y Yarom: Properties and distribution of ionic
conductances generating electroresponsiveness of mammalian inferior olivary neurones in vitro
- Katz, B, and R Miledi: A study of synaptic transmission in the
absence of nerve impulses
- BOYD, I A,and A R MARTIN: The end-plate potential in mammalian
muscle
- DEL CASTILLO, J, and B KATZ: Quantal components of the end-plate potential
To have more information on these resources, you can refer to the conclusion section where a more formal citation is provided.
Video credits
Writing: Tal Klimenko
Voice: Tal Klimenko
Animations: Tal Klimenko
Drawings: Tal Klimenko
Editing: Tal Klimenko
Introductory jingle: Thierry Du Sablond
Conclusion music: lukrembo - jay ( • lukrembo - jay (royalt... )