Episode 5: Making cross-sections by hand (stems)
Тәжірибелік нұсқаулар және стиль
This video provides a demonstration of making thin cross-sections by hand for plant material such as stems and roots; includes a demonstration of staining the material using TBO (toluidine blue O), a metachromatic stain useful for many plant materials. [See Episode 6 for how to prepare TBO.]
Пікірлер: 115
After watching this I realize, with a good instructor like you, there is nothing to worry about plant biology..
@BeyondtheBeanSeed
4 жыл бұрын
Thank you so much for this kind comment. It makes my heart glad.
I love how simple you made it, from showing the steps to explaining the purpose of each procedure.
Thank you Teacher Love from Kerala 🇮🇳 Helped me and my friend next week is our practical exam .Hope you will make more videos ❤️😊
Wow it's a perfect section💯. Nice video👌
@BeyondtheBeanSeed
2 жыл бұрын
Thank you!
Thank you teacher 🙏😊. For teaching the greatest method to prepare plant section slides 🌿🌿❤️❤️
@BeyondtheBeanSeed
4 жыл бұрын
Thank you so much!
I've always loved plant biology when I was a child, sadly (but probably for the better) that love eventually got pushed out by an interest in technology, however, now my (eighteen years younger) little sister got a microscope for Christmas and guess who's up at 4am to get everything ready for looking at cells? Yeah, it's me, and you're helping me out really well, thanks a lot! :)
@BeyondtheBeanSeed
5 жыл бұрын
Thank you! I'm so happy that we could be part of a special family time! I hope your sister is enjoying that microscope.
Wow!! after watching this video now I can do make cross section very easily...thanks miss
_Thank You Teacher For the Wonderful Session_ ❤️❤️❤️🙏 _Love From India (Kerala)_ 🥰💖 _This Video is Helpful For Kerala Class 12 Botany Practical Exam_ 🥰💯❤️⚡
@indiramv3352
2 жыл бұрын
🔥🔥🔥🔥🥰🥰
@BeyondtheBeanSeed
Жыл бұрын
You're most welcome!
Ohh my good...!!!love from india... You made me feel so much interest in biology
Thank you so much for this one ! Amazing this is ! Keep it up ! ✨
@BeyondtheBeanSeed
2 жыл бұрын
Glad you like it!
Please make some more videos! Your explanations are amazing!
@BeyondtheBeanSeed
5 жыл бұрын
Wow! Thank you so much. I have several episodes "on deck" just waiting for me to finish editing them. Stay tuned!
Love it I shall be doing this tomorrow at work! Much to the other bio techs annoyance!
Thank you so much for your clear idea👌
Thanks mam I think it will help me in my botany practical... Thanks alot..
That seems to be dicot.... Thanks for helping for my Biology Practicals
@BeyondtheBeanSeed
5 жыл бұрын
You are very welcome. Glad to help.
great simple video. thanx so much!!
Thank U this is a fast and easy method
@BeyondtheBeanSeed
2 жыл бұрын
Most welcome 😊
Superb explanation. I will be doing it tommorow in exam.
Thank you.It's very useful for me to study biology.🙏🙏🙏
@BeyondtheBeanSeed
4 жыл бұрын
Glad to hear that!
Thank you 💖
Thanks for the reference!
I actually get in trouble making thin sections. Gonna try this in next class for sure
Thankyou so much!!! Good experiment
So good. Your explanations are so amazing. But I'm hoping you could include a text in your observations. Thanks!!
Thanks miss for you wonderful explanation which helped me a lot ........
@BeyondtheBeanSeed
3 жыл бұрын
Glad to hear that
How do i get a cross section of the leaf? As in the mesophyll
Tnku..mam.. Tmrw ma lab xm it is very useful to see better.Than study.. ♥
Thanks for this video
Thank you mam....... it was really helpful...
amazing thank you for sharing
@BeyondtheBeanSeed
6 жыл бұрын
Thank you. More episodes will be coming soon.
Super! thanks!
Nice. Thank you
Love from our college ❤️
Excellent
Is the paper at 2:56 tissue paper or blotting paper. Thank you so much, bcoz of you I was able to do it properly
@BeyondtheBeanSeed
4 жыл бұрын
Sorry I did not see this comment in a timely fashion. I use laboratory wipes ("Kimwipes") for the blotting. I'm glad you found the video helpful.
Thankyou🤗👍👍
Can the same be done with ephedra stem????.coz ephedra is thin as compared to above plant
@BeyondtheBeanSeed
6 жыл бұрын
It should work just fine. Give it a try and post your results here! I've only had difficulties with stem cross-sections when they are from tissues that have lots of sclerenchyma fibers or are otherwise very tough. In those cases, I use essentially the same technique except that instead of holding the stem section in my hand and drawing the blade *towards* me, I place the stem to be sectioned on a cutting surface and slice downwards (like slicing fresh-baked bread). It is crucial to use a fresh blade (the blades I introduce in Episode 2, Red Bell Pepper) and to wet the blade and stem before cutting.
Thanks mam
Hello can you tell me please, can Isopropyl 9.99% be used instead of ethanol to rinse plant specimens after staining? and if so what percentage of water to Isopropyl should I use, or shoud I use Isopropyl in its purest form? Please excuse my ignorance, I am only just starting out with Microscopy, so at the moment I know very little.
This video so helpful. But I wanna ask u, what is the two types of its trichomes? And what is the advantage of making cross section than the other?
@BeyondtheBeanSeed
4 жыл бұрын
The plant shown in this video has both glandular and non-glandular trichomes. The glandular trichomes have a spherical cell at the top that can contain various compounds depending on the species. Both types of sections (longitudinal and cross) make visible the distribution of tissues within the plant stem but they differ relative to the "long axis" of the plant. The cross section is taken across (or perpendicular to) the long axis, and the longitudinal section is taken along (or parallel to) the long axis. Both types of sections are valuable, and both are required for a full understanding of the anatomy of the plant organ.
How prepare cellular structure of stem
What about slicing millions explants? Is there any machine to do million slicing of explant?
Very helpful
Thank you so much! Would you mind if i ask a one question? I bought a TBO to dye my plant, but it was in the form of powder. I'm curious what i should do to use it.
@BeyondtheBeanSeed
3 жыл бұрын
Here is my video showing how to prepare TBO Stain (and iodine potassium iodide); the written instructions are below the video. kzread.info/dash/bejne/eG1qp49xmbW0eaw.html
@user-hf9ob7mx3d
3 жыл бұрын
@@BeyondtheBeanSeed It was in the other video! Thank you very much!!
Thank you mam very helpfull
Thanks a lot.. My practical went really good
Very nice
Thnku
Tomorrow I have an exam and I hope this helps me!
Thanks i had subscribed ur channel
cool beans!
thank u sooo much miss
@BeyondtheBeanSeed
6 жыл бұрын
You are welcome. Please let me know what other techniques you would like to see!
@fahadansari8535
6 жыл бұрын
would u plz like to help me in slide preparation of epidermal layer of onion.
@BeyondtheBeanSeed
6 жыл бұрын
kzread.info/dash/bejne/aHxtlLCFhrDOo9Y.html
Tq for helping me ☺😊😊
@BeyondtheBeanSeed
4 жыл бұрын
You are very welcome!
please make video on seed LS and TS
Could you please show us how to make a section of monocot root? (I'll be happy if i get the videos of taking sections of all types of roots & stems)🙂
@BeyondtheBeanSeed
4 жыл бұрын
I hope to make a video of that soon. In the meantime, here is a verbal explanation for how I approach this. Roots are generally too thin and soft to section in the way we do stems; therefore, place the root on the slide and, using a fresh blade (like the one shown in the video), draw the blade across the root as though you were slicing a warm loaf of bread. You will not be pushing the blade down but instead will be slicing across the root (same motion as you do for the stem). I hope this helps.
@sreeram9589
4 жыл бұрын
Sure.... thank you very much.... expecting your video soon... ☺️👍
It is dicot or monocot..
It's good. I'd like to ask for permission for using this video in my class.
@BeyondtheBeanSeed
4 жыл бұрын
Yes, please use this for your classes. Thanks!
Super
Please why did you wet the blade and the material to be sectioned?
@BeyondtheBeanSeed
2 жыл бұрын
Wetting them helps ensure good contact and helps the blade glide while making the cut. Also, the film of water will "collect" the thin sections of plant material, which will adhere to the blade as the material is removed from the main section of plant material. I think it is good to keep the plant material hydrated throughout the process as much as possible to minimize damage and the inclusion of excess air bubbles.
👍👍
Plus Two Botany Practical Exam ന് വേണ്ടി വന്നവരുണ്ടോ? 🔥🔥🔥🔥
@indiramv3352
2 жыл бұрын
ഞാൻ ഉണ്ടേ 🔥🔥🤚🤚
@karthik_kk708
2 жыл бұрын
@@indiramv3352 😅🔥
Is it a dicot stem?
@BeyondtheBeanSeed
5 жыл бұрын
Yes, all of the examples in this video are dicot stems.
@adhirathdeshwal
5 жыл бұрын
Thanks
Hii hello am Sumitkumar SP Nice to meet you And nice your and useful thank you
Great Video, although it looks like a great way to also get a 'blood' sample at the same time 😂😂.. a ultra steady hand vital I reckon 😂
@melanie.link-perez
3 жыл бұрын
I'm happy to say that it isn't as scary as it might look, and (hopefully not jinxing anything) no students have been injured in the preparation of these sections. The movement with the blade is very small and controlled. I'm always impressed by how good the students' slides turn out! (I'm the Beyond the Bean Seed creator)
good
Hai iam Dipas
Please make videos , i am waiting 😊
@BeyondtheBeanSeed
2 жыл бұрын
I will try my best
@yukeshbabu0858
2 жыл бұрын
Ok mam i am happy😊
why is it desirable to cut it thinly?
@BeyondtheBeanSeed
4 жыл бұрын
For compound microscopy, you need samples to be thin so that light can pass easily through it. If sections are too thick, light will not be able to pass through and you won't be able to see details of the cells or tissues.
@pepsi3466
4 жыл бұрын
@@BeyondtheBeanSeed thanks :)
I think you should use brush for transferring the material
Don't use dissecting needle or any pointed thing to transfer section ,it may damage your section😐
Who's having practical tomorrow
@adnankhan-bv8ou
5 жыл бұрын
Me
@jmt8772
2 ай бұрын
Mine😂
kaufen sie sich doch mal ein gebrauchtes Ultramikrotom und machen Glasmesser. Die kosten etwa 300 Euro und vergessen sie diese Spielzeuge. microtome toys
T.lNARASIMHA REDDY
Malayali undou 8 std padikkunaa
@karthik_kk708
2 жыл бұрын
+2 Botany Practical Exam ന് വേണ്ടി വന്ന മലയാളി ഉണ്ട്. 😅🔥
i estimate that your sections are at least 200 my thic...senseless
Thanks mam
Thanks mam