Episode 5: Making cross-sections by hand (stems)

Тәжірибелік нұсқаулар және стиль

This video provides a demonstration of making thin cross-sections by hand for plant material such as stems and roots; includes a demonstration of staining the material using TBO (toluidine blue O), a metachromatic stain useful for many plant materials. [See Episode 6 for how to prepare TBO.]

Пікірлер: 115

  • @MrAndyroni
    @MrAndyroni5 жыл бұрын

    After watching this I realize, with a good instructor like you, there is nothing to worry about plant biology..

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    Thank you so much for this kind comment. It makes my heart glad.

  • @wanienoor
    @wanienoor Жыл бұрын

    I love how simple you made it, from showing the steps to explaining the purpose of each procedure.

  • @Gautham-nw5qp
    @Gautham-nw5qp Жыл бұрын

    Thank you Teacher Love from Kerala 🇮🇳 Helped me and my friend next week is our practical exam .Hope you will make more videos ❤️😊

  • @kaushalingalejaymaharashtr8766
    @kaushalingalejaymaharashtr87662 жыл бұрын

    Wow it's a perfect section💯. Nice video👌

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    2 жыл бұрын

    Thank you!

  • @NeonNova736
    @NeonNova7364 жыл бұрын

    Thank you teacher 🙏😊. For teaching the greatest method to prepare plant section slides 🌿🌿❤️❤️

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    Thank you so much!

  • @thecakeredux
    @thecakeredux5 жыл бұрын

    I've always loved plant biology when I was a child, sadly (but probably for the better) that love eventually got pushed out by an interest in technology, however, now my (eighteen years younger) little sister got a microscope for Christmas and guess who's up at 4am to get everything ready for looking at cells? Yeah, it's me, and you're helping me out really well, thanks a lot! :)

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    5 жыл бұрын

    Thank you! I'm so happy that we could be part of a special family time! I hope your sister is enjoying that microscope.

  • @sagarkumaryt
    @sagarkumaryt6 жыл бұрын

    Wow!! after watching this video now I can do make cross section very easily...thanks miss

  • @karthik_kk708
    @karthik_kk7082 жыл бұрын

    _Thank You Teacher For the Wonderful Session_ ❤️❤️❤️🙏 _Love From India (Kerala)_ 🥰💖 _This Video is Helpful For Kerala Class 12 Botany Practical Exam_ 🥰💯❤️⚡

  • @indiramv3352

    @indiramv3352

    2 жыл бұрын

    🔥🔥🔥🔥🥰🥰

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    Жыл бұрын

    You're most welcome!

  • @alpha758
    @alpha7583 жыл бұрын

    Ohh my good...!!!love from india... You made me feel so much interest in biology

  • @arushiagrawal7039
    @arushiagrawal70392 жыл бұрын

    Thank you so much for this one ! Amazing this is ! Keep it up ! ✨

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    2 жыл бұрын

    Glad you like it!

  • @irenecaretti7459
    @irenecaretti74595 жыл бұрын

    Please make some more videos! Your explanations are amazing!

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    5 жыл бұрын

    Wow! Thank you so much. I have several episodes "on deck" just waiting for me to finish editing them. Stay tuned!

  • @newworldorder8002
    @newworldorder80026 жыл бұрын

    Love it I shall be doing this tomorrow at work! Much to the other bio techs annoyance!

  • @jananisubharaman5135
    @jananisubharaman51355 жыл бұрын

    Thank you so much for your clear idea👌

  • @ruksharbanunirban8400
    @ruksharbanunirban84005 жыл бұрын

    Thanks mam I think it will help me in my botany practical... Thanks alot..

  • @punitabadodaria7043
    @punitabadodaria70435 жыл бұрын

    That seems to be dicot.... Thanks for helping for my Biology Practicals

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    5 жыл бұрын

    You are very welcome. Glad to help.

  • @2rivka
    @2rivka3 жыл бұрын

    great simple video. thanx so much!!

  • @plantphysiology6152
    @plantphysiology61522 жыл бұрын

    Thank U this is a fast and easy method

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    2 жыл бұрын

    Most welcome 😊

  • @surendhra265
    @surendhra2655 жыл бұрын

    Superb explanation. I will be doing it tommorow in exam.

  • @silviyaantony3339
    @silviyaantony33394 жыл бұрын

    Thank you.It's very useful for me to study biology.🙏🙏🙏

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    Glad to hear that!

  • @nayeem7359
    @nayeem7359 Жыл бұрын

    Thank you 💖

  • @10tus61
    @10tus616 жыл бұрын

    Thanks for the reference!

  • @sukanta6682
    @sukanta66825 жыл бұрын

    I actually get in trouble making thin sections. Gonna try this in next class for sure

  • @anwara9081
    @anwara90813 жыл бұрын

    Thankyou so much!!! Good experiment

  • @senku9798
    @senku97982 жыл бұрын

    So good. Your explanations are so amazing. But I'm hoping you could include a text in your observations. Thanks!!

  • @ambilyjiji5236
    @ambilyjiji52363 жыл бұрын

    Thanks miss for you wonderful explanation which helped me a lot ........

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    3 жыл бұрын

    Glad to hear that

  • @ohyeahyeah2427
    @ohyeahyeah24274 жыл бұрын

    How do i get a cross section of the leaf? As in the mesophyll

  • @truewords6233
    @truewords62335 жыл бұрын

    Tnku..mam.. Tmrw ma lab xm it is very useful to see better.Than study.. ♥

  • @PradeepSingh-db8ge
    @PradeepSingh-db8ge5 жыл бұрын

    Thanks for this video

  • @nilimaparamanik4181
    @nilimaparamanik41814 жыл бұрын

    Thank you mam....... it was really helpful...

  • @sAllu4m
    @sAllu4m6 жыл бұрын

    amazing thank you for sharing

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    6 жыл бұрын

    Thank you. More episodes will be coming soon.

  • @dmitriiishevskii6727
    @dmitriiishevskii67273 жыл бұрын

    Super! thanks!

  • @pdagaming7736
    @pdagaming77363 жыл бұрын

    Nice. Thank you

  • @allgamingvideosarehere
    @allgamingvideosarehere2 жыл бұрын

    Love from our college ❤️

  • @Ssrp_1996
    @Ssrp_19963 жыл бұрын

    Excellent

  • @lakshmisobha2739
    @lakshmisobha27394 жыл бұрын

    Is the paper at 2:56 tissue paper or blotting paper. Thank you so much, bcoz of you I was able to do it properly

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    Sorry I did not see this comment in a timely fashion. I use laboratory wipes ("Kimwipes") for the blotting. I'm glad you found the video helpful.

  • @merushabamon1763
    @merushabamon17634 жыл бұрын

    Thankyou🤗👍👍

  • @pokerater4463
    @pokerater44636 жыл бұрын

    Can the same be done with ephedra stem????.coz ephedra is thin as compared to above plant

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    6 жыл бұрын

    It should work just fine. Give it a try and post your results here! I've only had difficulties with stem cross-sections when they are from tissues that have lots of sclerenchyma fibers or are otherwise very tough. In those cases, I use essentially the same technique except that instead of holding the stem section in my hand and drawing the blade *towards* me, I place the stem to be sectioned on a cutting surface and slice downwards (like slicing fresh-baked bread). It is crucial to use a fresh blade (the blades I introduce in Episode 2, Red Bell Pepper) and to wet the blade and stem before cutting.

  • @kajalsingh5606
    @kajalsingh56063 жыл бұрын

    Thanks mam

  • @77MyEsha
    @77MyEsha2 ай бұрын

    Hello can you tell me please, can Isopropyl 9.99% be used instead of ethanol to rinse plant specimens after staining? and if so what percentage of water to Isopropyl should I use, or shoud I use Isopropyl in its purest form? Please excuse my ignorance, I am only just starting out with Microscopy, so at the moment I know very little.

  • @agnesyuwana3317
    @agnesyuwana33174 жыл бұрын

    This video so helpful. But I wanna ask u, what is the two types of its trichomes? And what is the advantage of making cross section than the other?

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    The plant shown in this video has both glandular and non-glandular trichomes. The glandular trichomes have a spherical cell at the top that can contain various compounds depending on the species. Both types of sections (longitudinal and cross) make visible the distribution of tissues within the plant stem but they differ relative to the "long axis" of the plant. The cross section is taken across (or perpendicular to) the long axis, and the longitudinal section is taken along (or parallel to) the long axis. Both types of sections are valuable, and both are required for a full understanding of the anatomy of the plant organ.

  • @martynmadhan5536
    @martynmadhan553610 ай бұрын

    How prepare cellular structure of stem

  • @bonduren887
    @bonduren8873 жыл бұрын

    What about slicing millions explants? Is there any machine to do million slicing of explant?

  • @Nibhaurboys
    @Nibhaurboys4 жыл бұрын

    Very helpful

  • @user-hf9ob7mx3d
    @user-hf9ob7mx3d3 жыл бұрын

    Thank you so much! Would you mind if i ask a one question? I bought a TBO to dye my plant, but it was in the form of powder. I'm curious what i should do to use it.

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    3 жыл бұрын

    Here is my video showing how to prepare TBO Stain (and iodine potassium iodide); the written instructions are below the video. kzread.info/dash/bejne/eG1qp49xmbW0eaw.html

  • @user-hf9ob7mx3d

    @user-hf9ob7mx3d

    3 жыл бұрын

    @@BeyondtheBeanSeed It was in the other video! Thank you very much!!

  • @muhsinsualih3935
    @muhsinsualih39352 жыл бұрын

    Thank you mam very helpfull

  • @TheSaneInternational-SNI
    @TheSaneInternational-SNI5 жыл бұрын

    Thanks a lot.. My practical went really good

  • @narendrasharma4898
    @narendrasharma48983 жыл бұрын

    Very nice

  • @piyushsoni3902
    @piyushsoni39025 жыл бұрын

    Thnku

  • @mustafizrahman284
    @mustafizrahman2845 жыл бұрын

    Tomorrow I have an exam and I hope this helps me!

  • @PrathameshShinde10
    @PrathameshShinde105 жыл бұрын

    Thanks i had subscribed ur channel

  • @joreenfajardo21
    @joreenfajardo212 жыл бұрын

    cool beans!

  • @fahadansari8535
    @fahadansari85356 жыл бұрын

    thank u sooo much miss

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    6 жыл бұрын

    You are welcome. Please let me know what other techniques you would like to see!

  • @fahadansari8535

    @fahadansari8535

    6 жыл бұрын

    would u plz like to help me in slide preparation of epidermal layer of onion.

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    6 жыл бұрын

    kzread.info/dash/bejne/aHxtlLCFhrDOo9Y.html

  • @d.srinivasulud.srinivasulu7731
    @d.srinivasulud.srinivasulu77314 жыл бұрын

    Tq for helping me ☺😊😊

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    You are very welcome!

  • @yogeshkandpal218
    @yogeshkandpal218 Жыл бұрын

    please make video on seed LS and TS

  • @sreeram9589
    @sreeram95894 жыл бұрын

    Could you please show us how to make a section of monocot root? (I'll be happy if i get the videos of taking sections of all types of roots & stems)🙂

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    I hope to make a video of that soon. In the meantime, here is a verbal explanation for how I approach this. Roots are generally too thin and soft to section in the way we do stems; therefore, place the root on the slide and, using a fresh blade (like the one shown in the video), draw the blade across the root as though you were slicing a warm loaf of bread. You will not be pushing the blade down but instead will be slicing across the root (same motion as you do for the stem). I hope this helps.

  • @sreeram9589

    @sreeram9589

    4 жыл бұрын

    Sure.... thank you very much.... expecting your video soon... ☺️👍

  • @shivanipracheta5551
    @shivanipracheta55514 жыл бұрын

    It is dicot or monocot..

  • @thitimonnithiyotdechawat7769
    @thitimonnithiyotdechawat77694 жыл бұрын

    It's good. I'd like to ask for permission for using this video in my class.

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    Yes, please use this for your classes. Thanks!

  • @afdal722
    @afdal7223 жыл бұрын

    Super

  • @ansahprincess5913
    @ansahprincess59132 жыл бұрын

    Please why did you wet the blade and the material to be sectioned?

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    2 жыл бұрын

    Wetting them helps ensure good contact and helps the blade glide while making the cut. Also, the film of water will "collect" the thin sections of plant material, which will adhere to the blade as the material is removed from the main section of plant material. I think it is good to keep the plant material hydrated throughout the process as much as possible to minimize damage and the inclusion of excess air bubbles.

  • @fayisvlog3884
    @fayisvlog38843 жыл бұрын

    👍👍

  • @karthik_kk708
    @karthik_kk7082 жыл бұрын

    Plus Two Botany Practical Exam ന് വേണ്ടി വന്നവരുണ്ടോ? 🔥🔥🔥🔥

  • @indiramv3352

    @indiramv3352

    2 жыл бұрын

    ഞാൻ ഉണ്ടേ 🔥🔥🤚🤚

  • @karthik_kk708

    @karthik_kk708

    2 жыл бұрын

    @@indiramv3352 😅🔥

  • @adhirathdeshwal
    @adhirathdeshwal5 жыл бұрын

    Is it a dicot stem?

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    5 жыл бұрын

    Yes, all of the examples in this video are dicot stems.

  • @adhirathdeshwal

    @adhirathdeshwal

    5 жыл бұрын

    Thanks

  • @Nibhaurboys
    @Nibhaurboys4 жыл бұрын

    Hii hello am Sumitkumar SP Nice to meet you And nice your and useful thank you

  • @coldeb8911
    @coldeb89113 жыл бұрын

    Great Video, although it looks like a great way to also get a 'blood' sample at the same time 😂😂.. a ultra steady hand vital I reckon 😂

  • @melanie.link-perez

    @melanie.link-perez

    3 жыл бұрын

    I'm happy to say that it isn't as scary as it might look, and (hopefully not jinxing anything) no students have been injured in the preparation of these sections. The movement with the blade is very small and controlled. I'm always impressed by how good the students' slides turn out! (I'm the Beyond the Bean Seed creator)

  • @Jerry01740
    @Jerry017403 жыл бұрын

    good

  • @Strikers83
    @Strikers833 жыл бұрын

    Hai iam Dipas

  • @yukeshbabu0858
    @yukeshbabu08582 жыл бұрын

    Please make videos , i am waiting 😊

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    2 жыл бұрын

    I will try my best

  • @yukeshbabu0858

    @yukeshbabu0858

    2 жыл бұрын

    Ok mam i am happy😊

  • @bethanymcmonagle9801
    @bethanymcmonagle98014 жыл бұрын

    why is it desirable to cut it thinly?

  • @BeyondtheBeanSeed

    @BeyondtheBeanSeed

    4 жыл бұрын

    For compound microscopy, you need samples to be thin so that light can pass easily through it. If sections are too thick, light will not be able to pass through and you won't be able to see details of the cells or tissues.

  • @pepsi3466

    @pepsi3466

    4 жыл бұрын

    @@BeyondtheBeanSeed thanks :)

  • @vinoddehariya1037
    @vinoddehariya10375 жыл бұрын

    I think you should use brush for transferring the material

  • @ifa1776
    @ifa17763 жыл бұрын

    Don't use dissecting needle or any pointed thing to transfer section ,it may damage your section😐

  • @alphonsapg3244
    @alphonsapg32445 жыл бұрын

    Who's having practical tomorrow

  • @adnankhan-bv8ou

    @adnankhan-bv8ou

    5 жыл бұрын

    Me

  • @jmt8772

    @jmt8772

    2 ай бұрын

    Mine😂

  • @lotharmayring6063
    @lotharmayring606311 күн бұрын

    kaufen sie sich doch mal ein gebrauchtes Ultramikrotom und machen Glasmesser. Die kosten etwa 300 Euro und vergessen sie diese Spielzeuge. microtome toys

  • @narasimhataniparthi4364
    @narasimhataniparthi43642 жыл бұрын

    T.lNARASIMHA REDDY

  • @raniyahmhomd2842
    @raniyahmhomd28423 жыл бұрын

    Malayali undou 8 std padikkunaa

  • @karthik_kk708

    @karthik_kk708

    2 жыл бұрын

    +2 Botany Practical Exam ന് വേണ്ടി വന്ന മലയാളി ഉണ്ട്. 😅🔥

  • @lotharmayring6063
    @lotharmayring606311 күн бұрын

    i estimate that your sections are at least 200 my thic...senseless

  • @tariqsheikh9400
    @tariqsheikh94008 күн бұрын

    Thanks mam

  • @svrbiology8274
    @svrbiology82745 жыл бұрын

    Thanks mam

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