Cell Thawing
Ғылым және технология
( www.abnova.com ) - The thawing procedures for regular (non-DMSO-sensitive) cells are performed. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells. More videos at Abnova www.abnova.com
Пікірлер: 6
Is this applied to cancer cell line too?
@kosheeka
24 күн бұрын
Yes, you can use the same cell thawing procedure for cancer cell too!
How I kmow DMSO will be under 1% after adding cells? How to do the calculation?
@kosheeka
25 күн бұрын
To ensure DMSO is under 1% after adding cells to suspension media. We are assuming that the freezing media is made up of 10% DMSO, 90% FBS. Identify volumes: • Let V1 be the volume of cell suspension. • Let V2 be the volume of cryovial freezing media added. Formula: • Final DMSO concentration = (0.1 x V2)/(V1 + V2) Inequality for DMSO • 0.01 > (0.1 x V2)/(V1 + V2) Simplified condition: • V2 Add less than one-ninth the volume of the cell suspension in freezing media to keep DMSO under 1%. For example, if you have 9 mL of cell suspension, add less than 1 mL of cryovial freezing media.