Can We Trust Western Blots?
Ғылым және технология
For more info, visit www.bio-rad.com/yt/V3/webinar-1
In this 45 min presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.
Western blotting is a widely used technique to identify and quantify proteins in complex samples. Unfortunately, as an editor of several journals, Prof. Gomes routinely observes Western blots that are not properly conducted. The generally low quality of western blotting data has led scientists to question whether we can trust protein quantification when using western blots. Reducing errors and misinterpretations requires standardized procedure and proper controls.
In this webinar, Prof. Gomes discusses the following key factors for high-confidence western blot data:
1) Quality of the primary and secondary antibodies and the blocking reagent
2) Proper target protein controls
3) Choice of total protein loading control vs. housekeeping protein loading control to normalize the target protein measurement
Prof. Gomes also presents tips to reduce the overall time required for first-rate western blots an how to minimize the pain of going through a long procedure. The scientific community needs to continue to increase the awareness of problems associated with western blotting and introduce, improve, and standardize basic criteria for western blotting practices so that the most investigators will be successful in eliminating common problems associated with "bad" western blots.
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Пікірлер: 17
This video is gold
This lecture actually upsets me for I don't realize that much possible problems... But still thans for sharing these info
Fellow Trinbagonian!! Currently at PSU. Good to hear a trini accent! Lol..missing my doubles:(
Basically, he just threw 10 months of my research to bin.
Thank you so much :)
Perfect!!!!!
Dear Dr Aldrin Gomez, I'm from Brazil. We have a big problem with the disease Glanders in Brazil. The serology confirmation is done with WB. Conflict in the results make us very concerned about the test. Lots of horses are been kill. How we can check if they are doing right? Mario Duarte
Changed my whole perspective on WB during my postdoc studies....
Wow. Such a detailed presentation! Do all these problems mean that an ELISA would be better for quantifying proteins?
@phatkaveh60
5 жыл бұрын
Duh....
Any new developments using PVA blocking? I've heard blocking can be achieved in less than a minute, but I am skeptical to try it out.
@patrickcrawley346
7 жыл бұрын
+
What concentration of tween 20 with TBS you use to block and dilute antibodies?
@thamizh2850
5 жыл бұрын
I tried to block nc membrane using tbst alone. But got dark blot. Why didnt work for me. Please give me your suggestion.
Hello. Can anybody tell me what he says at 10.42. ¨if you leave it .....of time¨ and 17:15 to 17: 29. Thanks
@pingerthestinger
9 жыл бұрын
Luis P. 10:42: This is identical samples. This is stained for just 1 minute with 5% BSA. This is stained for 1 hour with 5% BSA. 17:15: Talking about the amount of Ponceau S removed by water
@luisp.9710
9 жыл бұрын
Thank you.:)