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Great explanation. Very comprehensive summary with good animation👍👍👍

Great video, short and simple, nice graphics. Thank you!

Beautifully and quickly explained. Thanks for the video, you earned a sub!

Amazing explanation, right on the point. Thanks :)

I really like these kinds of videos where you understand every part of the topic. It was really helpfull.

Great video! Thank you for explaining it so fully.

This is super informative and easy to understand thank you

You way of teaching is amazing

Amazing crisp explanation. Thanks a lot

Helped a lot, many thanks!

Explanation was clear. thank you.

I just donated about 2 dollars for the video. But the video deserve much more than that

This was very useful thanks a lot!

Very clear explanation. Thank you 😊

Great explanation, thank you for your help

Thank u so much. This is helpful.

Thank you so much for clear explanation ❤

Awesome 🙏🏻 thank you

Thank you ,it was helpful.

Very nice video thanks for sharing

Nice channel and nice videos 👌

Amaaaaaazing thank u

so grateful sir

Thanks for this easy explanation!

@henrikslab

2 жыл бұрын

Thanks for watching!

well explained, thanks.

Very helpful.

Excellent

Thank you so very much

Good exp.

Well done explanation! But I have to confess that the "arrows" you showed ( C > C or C > T) looks like "greater than". 3:34

@henrikslab

2 жыл бұрын

I agree! This is a bit misleading..

Good.

I'm wondering how to confirm the specific T(which is the unmethylated cytosine) after PCR? And also thanks for your video which is clear and easy to understand!

@henrikslab

3 жыл бұрын

You wonder how distinguish between the “new“ T (unmethylated cytosine) and the “normal“ T in the DNA, correct? This works only by comparing the untreated DNA with bisulfite treated DNA... (sequencing) e.g. Untreated DNA A T C A G G Treated DNA A T T A G G the first T was originally also a T the second T was initially an unmethylated C

@dinushiarambegedara9906

2 жыл бұрын

@@henrikslab Do you know a way to compare the bisulfite converted sequence with a non-converted sequence? A software maybe?

@jinglin4358

9 ай бұрын

I know this question is a few years old but it’s possible to sequence whole genome with low concentrations of DNA ie extracted and not amplified DNA with next gen sequencing. So you can compare the sequence prior to bisulfite conversion and after.

My first like and first comment

@henrikslab

4 жыл бұрын

What an honor!

Well, how are we gonna use the same primers for the template whose Cs are changed to Ts in PCR?

Valeu!

@henrikslab

2 жыл бұрын

Not again... stop it, Mario! Thank you, man!

The video is very nice. However, I had not understood at first glance why U is converted into T in PCR. In another video, I saw that a complementary sequence is generated in a first-round, where U is paired with A. Then, in a second-round, the primary sequence is generated, but with T paired with A. It is equivalent to the conversion of U into T. I had confused because, in the cell, the U in DNA appears to be corrected into C. If the video showed the conversion of U into T in PCR it will be perfect!

@henrikslab

2 жыл бұрын

I agree! I decided to skip the step you mentioned ´cause I put the focus on the bisulfite conversion itself. But you described it correctly! I said "certain rounds of PCR" to simplify everything, but U is paired with A and this newly synthesized strand is paired with T then! True

love for that still

How do you know a given base was an un-methylated C and not a T? Do you to compare it the original sequence somehow?

@jinglin4358

9 ай бұрын

Yes. You compare post PCR sequence to pre. it’s possible to sequence whole genome with low concentrations of DNA ie extracted and not amplified DNA with next gen sequencing. So you can compare the sequence prior to bisulfite conversion and after.

I thought methylation mostly occurs at CpG islands alone?

@henrikslab

3 жыл бұрын

A CpG island is a region in the DNA with a high C G dinucleotide frequency and density. They are often located in promoter regions. Indeed, here we find an important part of methylation. You probably talk about CpG *sites*? Mainly methylation occurs at CpG sites, true. However, there is also methylation at CpA, CpT and CpG sites. See -> www.ncbi.nlm.nih.gov/pmc/articles/PMC5485512/

@alerookoh8748

3 жыл бұрын

Thank you for clarifying my question

My last like and last comment!

@henrikslab

4 жыл бұрын

Der Kritiker Ehre