Bioprinting 101: How to make Microfluidic Chips

Тәжірибелік нұсқаулар және стиль

Student In STEM Ria Bhatia demonstrates how you can create a pH gradient generator chip using microfluidics. Through this experiment, you will familiarize yourself with TinkerCad, a widely used 3D modelling software. Additionally, you will learn about microfluidics, which deal with the flow of liquids inside micrometer-sized channels, and receive an introduction to flow concepts, such as laminar flow. Throughout this project, you will also learn about acid-base reactions, which are indicators of pH value, and technical skills, such as ImageJ and Excel analysis.

Пікірлер: 47

  • @khaledfreij6586
    @khaledfreij65865 жыл бұрын

    Can i have more info about the dimensions of the channels?

  • @rock3tcatU233
    @rock3tcatU2333 жыл бұрын

    Is it advisable to use a vacuum pump in order to quickly remove the air bubbles in the PDMS?

  • @erwinz5926

    @erwinz5926

    2 жыл бұрын

    looks like they used one. propably it is. i thought the very same watching this.

  • @Muradsahar
    @Muradsahar2 жыл бұрын

    Amazing!

  • @danielayala646
    @danielayala6465 жыл бұрын

    cool! how many more times can the printed design be casted? or doesit disinttegrate aftyter first cast?

  • @SE3Dedu

    @SE3Dedu

    5 жыл бұрын

    It's one print per cast. Once you cast, it solidifies over time. Then you can wash off the channels.

  • @maxfreudenstein6892
    @maxfreudenstein68923 жыл бұрын

    The music gives me hope that som day our peple shall be free again

  • @weitingLin66
    @weitingLin665 жыл бұрын

    nice introduction! make it pretty clear

  • @mayasarilim3611

    @mayasarilim3611

    5 жыл бұрын

    Thank you for the feedback. Glad this was useful.

  • @PrasannaKumar-zx7gr
    @PrasannaKumar-zx7gr4 жыл бұрын

    Really interesting

  • @carlosvelazquez6440
    @carlosvelazquez64403 жыл бұрын

    Man, I'm just trying to figure out how colors mix to produce different colors, like is it linked to turbulance and if so is it a chemical reaction, or an optical illusion.

  • @theglobalwarming6081

    @theglobalwarming6081

    2 жыл бұрын

    The color is based on the pH of the fluid. I think they used one universal pH indicator for both samples. So, when they change color, they change due to a chemical reaction.

  • @zakyrayadhirh
    @zakyrayadhirh3 жыл бұрын

    can I get a paper from your research?

  • @RuyRuiz
    @RuyRuiz11 ай бұрын

    Love it, omg you really make sounds so easy. New Subscriber.

  • @a.n.9227
    @a.n.92274 жыл бұрын

    Hi can you send me a quotation of prices of the reagents you used? PDMS, Fpluronic, ... or even redirect me to someone who could do so. thanks a lot

  • @PrasannaKumar-zx7gr
    @PrasannaKumar-zx7gr4 жыл бұрын

    👍

  • @kryogenica4759
    @kryogenica47595 жыл бұрын

    Are the channels exposed to the open air on the bottom face?

  • @SE3Dedu

    @SE3Dedu

    5 жыл бұрын

    Yes we use tape to cover the channels so you have a seal to flow fluids through. Otherwise you can use a glass slide.

  • @kryogenica4759

    @kryogenica4759

    5 жыл бұрын

    @@SE3Dedu Thank you so much for the info! Will see if these can be implemented in a classroom.

  • @milascatadventures1011

    @milascatadventures1011

    5 жыл бұрын

    @@kryogenica4759 If you go to portal.se3d.com and sign up for a free account, you can find our print files and instructions there, under Additional Resources, Bioprinting Project Kits.

  • @visionbios6736
    @visionbios67364 жыл бұрын

    Any recommendations on how to attach the PDMS device onto a glass slide ? Any help is much appreciated

  • @mayasarilim3611

    @mayasarilim3611

    4 жыл бұрын

    Just make sure both surfaces are clean and dry before attaching the glass slide onto the PDMS cast. Good luck!

  • @Amyhb69

    @Amyhb69

    3 жыл бұрын

    Plasma

  • @sharadambardar9252

    @sharadambardar9252

    2 жыл бұрын

    oxygen plasma treatment

  • @bhatt6873

    @bhatt6873

    Жыл бұрын

    You have to go for O2 plasma etching of the glass slide after which you bond it with the PDMS

  • @amitasthana45
    @amitasthana45 Жыл бұрын

    can you please send us the dimension or STL file to me

  • @aslcatalyst416
    @aslcatalyst4165 жыл бұрын

    Hi is the CAD for this part available for download?

  • @SE3Dedu

    @SE3Dedu

    5 жыл бұрын

    Pls visit portal.se3d.com and sign up for free to access files, under Additional Resources, you will find it under Bioprinting Project Files

  • @baltakatei

    @baltakatei

    2 жыл бұрын

    @@SE3Dedu lol, it's dead.

  • @Farhan-co9he
    @Farhan-co9he Жыл бұрын

    impressive

  • @lintong8703
    @lintong8703 Жыл бұрын

    Pluronic 127 for creating the mold / channels

  • @nebuchadnezzar47
    @nebuchadnezzar474 жыл бұрын

    why dont they just completly 3D print the end product in plastic? with channels built in?

  • @TheMrJuoji

    @TheMrJuoji

    4 жыл бұрын

    depending on the kind, but fdm is out because of the porosity , i don't know for resin printing but i guess you'd need something chemically inert , as for slm/sls, it's probably feasible yet would be an expensive piece of equipment

  • @ignacioaguirrenoguez6218

    @ignacioaguirrenoguez6218

    11 ай бұрын

    They are doing that nowadays

  • @erwinz5926
    @erwinz59262 жыл бұрын

    so- the result is green yellow!!!11! *scnr.* : )))

  • @pubgamedz
    @pubgamedz Жыл бұрын

    Whey i Can't download! 🛸

  • @vivianaclaveria9123
    @vivianaclaveria91233 жыл бұрын

    This is not microfluidics. This is millifluidics. Nice video and technique anyway.

  • @SE3Dedu

    @SE3Dedu

    3 жыл бұрын

    Yes you are absolutely correct. More for demo and educational purposes.

  • @Karpe_Deem
    @Karpe_Deem3 жыл бұрын

    3:44 minutes for a fraction of a second of demonstration 😢

  • @miron__
    @miron__5 жыл бұрын

    fix your audio

  • @maracachucho8701
    @maracachucho87014 жыл бұрын

    Not very "micro", though!

  • @wirehyperspace
    @wirehyperspace3 ай бұрын

    Lego land

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    @georgen9755 Жыл бұрын

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    @georgen9755 Жыл бұрын

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  • @mdgar6516
    @mdgar65164 жыл бұрын

    quit the music, it is irritating

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