Hi..the video is great..but you have made a mistake with size exclusion chromatography..the molecules that are larger in size (the ones excluded from the beads due to larger size) are the first ones to elute out followed by the small, the smaller and the smallest..the reason being that the molecules which can enter the beads via the pores have a longer path and hence a longer transit time.

@5Youssif

7 жыл бұрын

upvote so others see

@iansteinke2414

6 жыл бұрын

That is a different concept in general. So im not entirely sure this applies. Correct me if im wrong, but in liquid chromatography size is a factor yes, but polarity is the main concern here.

@EDERDUDE

2 жыл бұрын

Correct

The point on size chromatography is wrong. Larger size elutes faster as it can go around the beads, whilst smaller beads have greater retention as it has to go through the intriciate pores.

@peepdi

5 жыл бұрын

True that.

@peepdi

5 жыл бұрын

@@hargobindsingh3167 oh is it? Cause even we studied about the larger ones eluting fast.

@hargobindsingh3167

5 жыл бұрын

@@peepdi sorry was wrong here mixed a concept😅

@peepdi

5 жыл бұрын

@@hargobindsingh3167 it's completely fine 😁

@robertsanchez4316

5 жыл бұрын

This is indeed true. Smaller molecules travel through the porous beads thereby making their path to elute much larger than that of larger molecules. I am an advance biochemistry student lol

Shout out to you! Thank-you for your simple yet complex explanations because I completely understand this topic now and feel confident to write my extended response!

* correction, it should say: in size- exchange chromatography, small particles get trapped in the column by the small beads, thus small particles are slower to move. I just read that in Kaplan MCAT biochem review.

That moment when a 4th grader becomes your teacher :P...

@remusomega

8 жыл бұрын

+Gemechu Bekele The moment you fail verbal reasoning because you can't take a joke

@sylvecakes8450

8 жыл бұрын

+Gemechu Bekele how do you know it is not a fake channel?

@nathanbrown4689

7 жыл бұрын

+Gemeche Bekele, that joke not only flew over you, it fucking soared past at fucking mach 6 lol

@sylvecakes8450

7 жыл бұрын

I did not type that it was my friend for reals

@saintofchelseathomascarlyl5713

6 жыл бұрын

it was my friend

6:28 In size exchange(exclusion) chromatography, aren't they the bigger molecules that move quicker than the smaller ones because the smaller ones have to travel in the beads?

@easylearning981

8 жыл бұрын

+Gemechu Bekele Yes.

@khadijahadam4387

8 жыл бұрын

Yup agree. In size exchange chromatography if the size is too big it wont pass through the beads instead it will flow through the column. Only small particles will flow through the beads hence, smaller particles is said to have higher retention time

@pranavvariava2685

7 жыл бұрын

emeg losota yes

@VincentStevenson

6 жыл бұрын

It's the same principle as DNA Gel electrophoresis, there's no limit to the length of DNA that can pass through the gel. Really long molecules will travel with smaller ones through the silicone, but much slower.

@ND-ji8dk

2 жыл бұрын

@@VincentStevenson no not same principle, larger DNA pieces travel less further due to interactions with agarose gel. whereas in SEC larger elute out first/travel faster as they do not get stuck in the holes of the bead

That was extremely helpful, thanks!

You totally sound like Alphonse Elric from Full Metal Alchemist: Brotherhood. Amazing video, by the way. Thank you.

@A_Dopamine_Molecule

6 жыл бұрын

You're absolutely right, I can't unhear it now :/

@firephoenix8192

6 жыл бұрын

Absolutely.

@kasondemulenga3472

6 жыл бұрын

Oh my God yes she does!

@ekhs2669

3 жыл бұрын

When your a weeb and u spent all the time watching anime and now is studying 1 hour before the exam

extremely helpful :) love your voice :)

I had no idea what it meant, but then I realised I did this at primary! Thanks

Thank you so much ! Have exams soon and this assisted me :)

Thank you, very informative!

Thank you soooooo much. This will definitely help me with my lab

Soo beautiful voice And you explan clearly Thanx for sharing knowledge.... 🙂

thank you so much those extremely helpful!

Thanks for the helpful video..I've made a simple at-home experiment involving paper chromatography but instead of drawing a dot..I drew a line with a black pen used for whiteboard..and it separated to 3 colors as I remember..and I used a mixture of water/ethanol to get the best separation!

thank you. this was very helpful.

Aww, her voice is so cute

this video made it make so much sense THANK YOU

Great video, super helpful thanks

Thanks, very helpful.

thanks helped alot!

u just saved my life thank you so much

Beautifully Explained!

me to thanks got a test common up and this helped me :)

yes you are right and like

khan academy is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on basics on chromatography.

Thanks girl ! The best here 👏👏👏👏👏👍👍👍👍👍😙

Thank you.

Nice video really helped before exam thanks you so much

her voice is so soothing

excellently explained !

this was helpful. thank you!

this video help me so much... tq :)

Thank you so much for making the video. If possible, could you please change the font color in the future? As It is quite difficult to read your note toward the end of the video. Thank you again for your time

This helped for science olympaid thanks!!

@legendarypanda424

4 жыл бұрын

Wait! Were you doing crime busters?

Thank you so much

Thank You very much

What happened to Sal's voice? 0_o

@WaddiaS

8 жыл бұрын

It's a KA spin-off

@lovelybias

8 жыл бұрын

+ChimChim I just scroll the page to see some of the comments idk why and I saw your current profile pic... BTS is following me even in my hw ;o;~

@nusaibahhussain9975

7 жыл бұрын

Ikr?

@audreyandremington5265

3 жыл бұрын

He started listening to Jo Jo Siwa and then his voice changed. It's sad. Don't listen to Jo Jo Siwa, kids.

I think that you have it backwards in Size Exclusion Chromatography. The larger molecules can travel faster because they are not distracted by the other paths that can be taken to reach the end of the column.

@chriskoperniak784

8 жыл бұрын

+Joseph Landavaso No, she's right - the larger molecules travel slower

@naomik5213

8 жыл бұрын

+Chris Koperniak in size exclusion chromatography the large molecules elute quicker. The small ones get stuck in the beads. She is wrong.

Thanks a lot 🙏

Very very helpful ❤️

very nice... thanks a lot for the video. ..

Great video.. more information in least time..✨✨✨

Should probably mention most often for HPLC the silica gel is bound to a nonpolar group, so the more polar group elutes first v. all other techniques the more nonpolar group elutes first.

@Nymeria0

5 жыл бұрын

Doesn't that depend on the solvent that is used? In most cases, it would be polar solvent, hence the most polar compound would elute first.

Most underrated channel

x1.5

@thefattyfatty1

8 жыл бұрын

+MrElectricSkittles Expert call right here

@mertarican5456

5 жыл бұрын

thx

@ryusworld2701

3 жыл бұрын

Hi yall

@michaelladenice

3 жыл бұрын

ye i really got annoyed and changed the playback speed

thank you for the great content.

Than you very much for the help. the way you explained paper chromatography made it extremely simple. Again, thank you for such a video

I love it 😍

Love your pictures. They are GREAT: )

Thank you !! Extremely helpful

@prit0012

2 жыл бұрын

our comments look pretty young out here 🤣.once upon a time Khan Academy used to be a very popular educational channel

This video is awesome ❤️‍🔥❤️‍🔥❤️‍🔥, I understood completely

Thank youu

Helping me out for my regents today ☹️✅

Thanks this video helped me a lot!

@abdullahrahman9883

3 жыл бұрын

kewl

Fantastic ❤

In paper chromatography, the stationary phase is not the paper but water adsorbed into the paper

4:32 COMMENTATORS SHOUTING : W...W...E.... Tables Ladders and Chairs TLC.

where is this on the website?

The stationary phase is actually water trapped between the cellulose fibres of the paper.

so, the capillary action will take place in both?

Does anyone have some videos I can use for science Olympiad

everyone is either 1) sexualizing the speaker 2) criticizing and comparing the speaker to Sal... what does that even say about you guys? Lovely video, they're just jealous you understand chromatography and they don't.

Can u plz make a tutorial on ascending paper chromatographyy

Interesting. Seem to be a few accuracy issues though. But it's a good video

10/10 ign most intrtuiging thing ever

you should choose a better pallette of colours, some couldnt read what you wrote in the hplc, like me.

In paper chromatography, the stationary phase is not paper but the liquid that is bonded upon it.

That sounds like evalion. Would be pretty cool if she is doing chemistry now.

very good

nice work keep it up

kindly add true expt. which would be easy to understand

very nice

Very good and simple explanation,thanks :))

Thx ima make ricen now

@epic8924

2 жыл бұрын

Came here from the video too

Good drawings, but needs to concentrate and remember the speech

Bro this video helped a lot with my science assingment in Year 7!

@Philippine_Navyist16

9 ай бұрын

same

@TheChessRobloxian

9 ай бұрын

@@Philippine_Navyist16 I got a B+ for this video! I could've gotten an A if I hadn't gone over time!

@Philippine_Navyist16

9 ай бұрын

@@TheChessRobloxian todya is teachers day and no school, i dont know if my science teacher will approve this tom. Wisj me luk

@TheChessRobloxian

9 ай бұрын

@@Philippine_Navyist16 wdym ur science teacher won't approve? anyways good luck.

@Philippine_Navyist16

9 ай бұрын

@@TheChessRobloxian yay he aprovves

You are explaining GC as if it is fractional distillation.

Hi fbi personnel I'm not makeing ricen I would die doing it and I have no uses for it

What is cpillary action

Nice

danke

so which substance is more polar? The one closer to the stationary or mobile phase?

@SantosAl

7 жыл бұрын

Theodore Goujon. depends on the the type of stationary phase/mobile phase you use.

good

I m in love with your voice😀😀😆 And yes this was very useful thanks!!!

is lipid molecule separated with the help of gas exchange chromatography?????????????

Stationary Phase in case of paper chromatography is liquid, because it has molecules of Water.

Munna sir's advice.

Cute voice.

just to clarify things out, this is paper chromatography right????????

Aren't the water molecules trapped WITHIN the cellulose fibres of the paper the stationary phase and NOT the paper itself?

@medschoolvideos

8 жыл бұрын

+Mamma Harun yh but it better to say the paper as the cellulose is needed to keep the water stationary

5/5, would watch again

wow thanks pushed my grade from an f to an A*. I'm gonna now major in chemical engineering to make the fortnite chug jug real

Cute voice

is her nose blocked or something? but good video

beautiful voice.

@yarakharam5343

6 жыл бұрын

are fucking kidding me she sound slike a 7 year old boy with a clogged nose. You must be some fucking omega-virgin.

@TheRayll

6 жыл бұрын

and this is why i love reading comments

@Abdullah-cj2mh

6 жыл бұрын

Nice Student idk why but "some kind of fucking omega-virgin" made my day

@TheRayll

6 жыл бұрын

ikr

@yarakharam5343

6 жыл бұрын

ty m8 I really felt it while typing

Can someone please explain how paper chromatography relates to spotting fake paintings? If the colors travel farther then does it mean that the painting's fake or the other way around?

@InLostTime

7 жыл бұрын

Old paintings used colours that were different in composition to those that are used now because alot of old paints were carcinogenic and toxic, they perform the chromotography stahe and the dissolved paint will elute at a certain time which is recorded. This is known as the retention time, and can be considered a fingerprint of the molecule as it is unique for every compound. This retention time is then compared to a database of compounds with known retention times and will display compounds that correspond, they then can check the manufacture date of the compound to assess the age of the painting and therefore distinguish whether the painting is real or fake :)

There are two principles on chromatography~ 1) Adsorption 2) 2 phases (Stationary and moving) Am I right?

@akaruiorenji5287

7 жыл бұрын

Ishu 1) adsorption 2) partition

@ishu9176

7 жыл бұрын

Akarui Orenji Oh yeah thanks :3

@georgemalpus7634

6 жыл бұрын

Ishu Cool

cute voice