Amino acid charge at different pH and amino acid titration calculation problems

Amino acid charge at different pH and amino acid titration calculation problems - This lecture explains about the amino acid titration curve and this will teach you the process of calculating amino acid charge at different pH along with pka values and it will also help you to know the net charge of polypeptide chain from the pka values of amino acids.
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Thank you for watching the video lecture on amino acid titration curve and net charge problems.
This will also teach you about the amino acid charge pH and Pka values and the way to calculate net charge of amino acid.

Пікірлер: 325

  • @nahomelion
    @nahomelion3 жыл бұрын

    His calm demeanor, how he talks, and his accent are some of the qualities of this man. Thank you, sir!

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    3 жыл бұрын

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  • @Yakshiii
    @Yakshiii2 жыл бұрын

    this man is a king, explained so concisely what my professor could not explain in two hours... thank you sir!

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    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @pntdnails8835
    @pntdnails8835 Жыл бұрын

    just got out of COVID quarantine and my prof didn't explain this at all and the figures were making it really confusing. I skipped ahead to the part I was confused and it took literally a minute and I completely understood this topic. thank you so much for this video that you created like 5 years ago

  • @nephronum
    @nephronum5 жыл бұрын

    Can you explain why did you put carboxyl group in ionized state, whereas amino group was not ionized.(in cell conditions both are ionized)

  • @mdbadsha418
    @mdbadsha4182 жыл бұрын

    You are the best! I never understood this topic so clearly before. Same for many other topics you taught in your other videos. Thank you so much!

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    @shomusbiologyofficial

    2 жыл бұрын

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  • @ujalaabbas1861
    @ujalaabbas18615 жыл бұрын

    Thanks for the video.. cleared the concept ❤️❤️👍👍

  • @YourManasi
    @YourManasi3 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @pallabisarma8463
    @pallabisarma84636 жыл бұрын

    Thank you is a very small word for your videos sir.. You are great

  • @ranarajeshwariba1120
    @ranarajeshwariba11206 жыл бұрын

    Sir me apke video se hi hamara pura syllabus ache se samaj me aa jata he so tnx and vert good work and very helpful for our study

  • @thisariupamalika9924
    @thisariupamalika99245 жыл бұрын

    Thank you, Sir. Your videos are really helpful for me to understand the concepts of biochemistry. And I'd strongly recommend to anyone to watch your videos to clear their doubts.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

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    @vishnuparihar5445

    4 жыл бұрын

    Ok

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    @vishnuparihar5445

    4 жыл бұрын

    Thisari Upamalika ok

  • @akankshabhatt3357
    @akankshabhatt33573 жыл бұрын

    I had a hard time trying to understand this simple concept and finally it all made sense. Bless your soul for making such content, you are helping so many people including me to understand basic fundamentals in biochem/biology/chem.

  • @dailydevosinc.4345
    @dailydevosinc.43453 жыл бұрын

    you've enlightened my understanding better. Thank you very much SIr

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome

  • @Ceeceelu_
    @Ceeceelu_3 жыл бұрын

    Thankyou for simplifying this to me I definitely feel more confident for my exam.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome

  • @1rrrrrrrrrrr544f
    @1rrrrrrrrrrr544f Жыл бұрын

    Eventhough there was a mistake,but thanks because you made me sure about my idea! I concluded the same thing when I studied amino acids alone!

  • @dayangasyiqin2398
    @dayangasyiqin23985 жыл бұрын

    great job!

  • @Empress426
    @Empress4262 жыл бұрын

    Thank you for giving me some clarity on this topic, I am currently taking a self-taught biochemistry class with a very confusing professor. Thank you.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

  • @skp-official
    @skp-official6 жыл бұрын

    Your videos helped me lot in class 12

  • @makayla1774
    @makayla1774 Жыл бұрын

    You made this so simple, thank you.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    You're welcome

  • @siddharthsingh5169
    @siddharthsingh51693 жыл бұрын

    Shomu Sir , i like your personality and also the way of talking. wow

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    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @mashab6374
    @mashab63743 жыл бұрын

    Thank you so much!! It's so clear to me now!!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome

  • @rajwinderdubb3222
    @rajwinderdubb32223 жыл бұрын

    Omg Thank you so much sir! you explained it very well

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @dikshajha8279
    @dikshajha82793 жыл бұрын

    Thankyou sir for making the concept easy to understand 🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome

  • @kavyap4453
    @kavyap44533 жыл бұрын

    Thank you so much for this very simple and informative lecture

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you. Glad you liked my lectures

  • @Anjalish66
    @Anjalish666 жыл бұрын

    You are doing great job sir.. God bless you

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    @shomusbiologyofficial

    6 жыл бұрын

    +Anjali Shakya thank you. Glad you liked my lectures

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    @CrestScienceAcademy

    6 жыл бұрын

    Anjali km yes

  • @CrestScienceAcademy

    @CrestScienceAcademy

    6 жыл бұрын

    Hi

  • @rachna4140
    @rachna41406 жыл бұрын

    sir can u make video on longpolypeptide chain like this. plzz soon becoz it gives idea how can calculate

  • @andreusdagoat9958
    @andreusdagoat99584 жыл бұрын

    Wow KZread has the best teachers!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you

  • @taitopotato
    @taitopotato2 жыл бұрын

    Legend! Thank you so much.

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    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

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    @theimprovist41582 жыл бұрын

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    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @ankitaarvindbhai1059
    @ankitaarvindbhai10594 жыл бұрын

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    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

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    @prakashpawar15034 жыл бұрын

    Nice🔥🔥🔥

  • @kavitagarg4656
    @kavitagarg46563 жыл бұрын

    Too good explaination and easy to understand

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    @shomusbiologyofficial

    3 жыл бұрын

    Thank you. Glad you liked my lectures

  • @giftnamwanja7715
    @giftnamwanja77155 жыл бұрын

    awesome thanks. God bless

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    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @gargisain5091
    @gargisain50913 жыл бұрын

    Your explanation is too much good sir ♥

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    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @meenublossom6860
    @meenublossom68605 жыл бұрын

    Awesome lacture... 👌👌Thank you so much for clearing my concepts with amino acid charge calculation 🙏🙏😊

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @pierrejeanes

    @pierrejeanes

    5 жыл бұрын

    @@shomusbiologyofficial multipel ?

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    @pierrejeanes

    5 жыл бұрын

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  • @somnathchanda1250
    @somnathchanda1250 Жыл бұрын

    will de-protonation of amine happen at pH 10 for developing a negative charge over -NH gr.

  • @raviphougat2474
    @raviphougat24742 жыл бұрын

    Excellent sir God bless you

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you

  • @philomax5657
    @philomax56576 жыл бұрын

    thnks pro

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    +Biologie facile you're welcome

  • @enashaswa613
    @enashaswa6132 жыл бұрын

    where can i find part 2? it's not shown to me ,if someone could put its link i'd be thakfull

  • @farooha.s8411
    @farooha.s84112 жыл бұрын

    Thanks you so much!! Where can I see the second part of the class? I need to know how to calculate the net charge for a polypeptide

  • @janvimyadventures1310
    @janvimyadventures13104 жыл бұрын

    Sir I have some confusion with glycine formula. In the video it is NH - CH2- COO-. Is it correct.

  • @soumyajitdas6686

    @soumyajitdas6686

    3 жыл бұрын

    Yrs

  • @abdulharrison4380
    @abdulharrison43802 жыл бұрын

    Thank you soooooooo much Sir!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

  • @fizaali6039
    @fizaali6039 Жыл бұрын

    bro ur the best

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    @shomusbiologyofficial

    Жыл бұрын

    Thank you

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    @Rajkumar25679 Жыл бұрын

    Tqsm sir ❤️

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    @shomusbiologyofficial

    Жыл бұрын

    You're welcome

  • @israthossain6012
    @israthossain60124 жыл бұрын

    For Glycine when pH is 12 how does the net charge is -1? In N-H because of the deprotonation the charge is -1 and in COO- bond there is -1 charge so the total net charge is supposed to be -2. When pH=7 you are counting the charge of COO- is -1 to get the total net charge. Then why when pH=12 you are not counting the charge of COO- group -1 charge to get the total net charge? Can you please explain it?

  • @lubnasa3453
    @lubnasa34532 жыл бұрын

    thank you sooooo much man

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

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    @philasandexulu33044 жыл бұрын

    Thank you 🤩

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @misbashabir2063
    @misbashabir2063 Жыл бұрын

    Thank you sooo much sir .....

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    @shomusbiologyofficial

    Жыл бұрын

    You're welcome

  • @juhishrivastava8725
    @juhishrivastava87255 жыл бұрын

    Very informative sir.

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    @shomusbiologyofficial

    5 жыл бұрын

    Thank you for appreciating my efforts

  • @rudraseth5419
    @rudraseth54196 жыл бұрын

    Sir could you plz elaborate how the molecule are protonated and deprotonated .

  • @Mr.Blue.Sky.i
    @Mr.Blue.Sky.i2 жыл бұрын

    Thank you!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

  • @Sunshine-Muskan
    @Sunshine-Muskan3 жыл бұрын

    Where can I find the second video

  • @YashSharma-ce1hk
    @YashSharma-ce1hk2 жыл бұрын

    Sir, I'm a JEE aspirant and I recently faced a problem on Biomolecules The question had three phases of glycine where [A]- Both acid part and -NH part was protonated [B]- Only the -NH part was protonated [C]- Both the parts was deprotonated pKa1=2.3 pKa2=9.7 The question asked if at pH=2.3, half of [A] is converted to [B] or not? So sir, can I consider it to be correct that yes it is converted, on the pretext that half part is protonated and half is deprotonated? (I am having confusion in the part where it asked 'converted', and I am not sure that half protonation would result in half conversion of phase [A] to phase [B])

  • @sambhavmishra1873
    @sambhavmishra18736 жыл бұрын

    Hello sir, i have a question whether the Pka value would be given in CSIR-net question or we have to memorize??

  • @highrepublic3264
    @highrepublic32642 жыл бұрын

    thank you for this video friend

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

  • @shyambhavi10
    @shyambhavi103 жыл бұрын

    Thank you sir 👍👍....

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    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome.

  • @wycliffenyandika9017
    @wycliffenyandika90172 жыл бұрын

    Damn! You have no idea how long have been going through videos on KZread and still couldn't answer a question on this topic ,I'm preparing for MCAT, this will go a long way. Only way to pay you is to share the video

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    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @riusgallery3397
    @riusgallery33974 жыл бұрын

    Thank you sir🙏

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    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @prasadmadane9711
    @prasadmadane97113 жыл бұрын

    Great sir🤗

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    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @nitinchauhan9075
    @nitinchauhan90756 жыл бұрын

    Your videos are really helpful...I m totally dependent on ur videos. Thanks a lot...you are doing a great work

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    @shomusbiologyofficial

    6 жыл бұрын

    +Nitin Chauhan thank you. Glad you liked my lectures. Please subscribe and share

  • @nitinchauhan9075

    @nitinchauhan9075

    6 жыл бұрын

    Shomu's Biology : No concept is difficult with your way of teaching.

  • @sanjaisrao484
    @sanjaisrao4842 жыл бұрын

    Excellent explanation

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you

  • @sanjaisrao484

    @sanjaisrao484

    2 жыл бұрын

    @@shomusbiologyofficial welcome

  • @sohilausamarabie9631
    @sohilausamarabie96313 жыл бұрын

    THANK YOU!!!!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @054gomathip7
    @054gomathip73 жыл бұрын

    When I get confused in some topic in biology ,I usually watch your videos, it gives clarity.tq sir

  • @rabianoor1589
    @rabianoor15893 жыл бұрын

    Thank you so much sir i completely understand this topic🥰😅

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @somnathchanda1250
    @somnathchanda1250 Жыл бұрын

    Oh! what an explanation

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Thank you

  • @suro-mallar8031
    @suro-mallar80313 жыл бұрын

    In case of titration of a peptide chain, should we start from the fully protonated form of the peptide or the fully deprotonated form?

  • @elvishernanserrano7736
    @elvishernanserrano7736 Жыл бұрын

    Where I can find the second video for polypeptide?

  • @hasankuliyev4211
    @hasankuliyev42115 жыл бұрын

    is net charge would be -2 at pH 12 since there is already -1 (pH 12 does not change -1 and -1 stays -1) in carbonyl group and very basic condition(pH 12) rips out H from NH which makes -1 as you mentioned, So -1 + -1 =-2 isn`t it?

  • @mytwinze942

    @mytwinze942

    4 жыл бұрын

    I agree with you ,we need clear this point

  • @sudiptadhara1983
    @sudiptadhara19836 жыл бұрын

    very informative

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    Thank you

  • @sudiptadhara1983

    @sudiptadhara1983

    6 жыл бұрын

    sir I am a student of Physiology(b.sc)...IIT jam ka kaise preparation lena chahiye..please inform me.....

  • @surajbhowmik4404
    @surajbhowmik44043 жыл бұрын

    sir how to find the next class?

  • @pixiepout
    @pixiepout4 жыл бұрын

    Thank you very much for this video it really helped with my studying!!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @umaverma9437
    @umaverma94372 жыл бұрын

    The pI of glycine is written as 5.97 in books. Is that correct if we take ph 5.97 instead of 7 in this calculation? In books the formula for pI is , 1/2 (pK1+ pK2) i.e., 1/2 (2.34+ 9.6) = 5.97

  • @shonalikrishnani8775
    @shonalikrishnani87756 жыл бұрын

    sir please solve the analytical part of csir net 2017.. your videos help a lot.. thank you so much sir.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    +Shonali Krishnani I will

  • @omethheenkenda2793
    @omethheenkenda2793 Жыл бұрын

    thank you

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    You're welcome

  • @hamidazizi7658
    @hamidazizi76583 жыл бұрын

    thanks man, Greetings from Germany :D

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @noone-kd2jd
    @noone-kd2jd3 жыл бұрын

    Thank you sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome

  • @priyapriya.c.g2755
    @priyapriya.c.g27554 жыл бұрын

    Thank you souch sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @Sahil-fo9nu
    @Sahil-fo9nu3 жыл бұрын

    Sir, it means for glycine pI ranges from ~3 to 9 ?

  • @sjc5411
    @sjc54113 жыл бұрын

    He taught the subject better than my professor

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @bishwabhusankundu2385
    @bishwabhusankundu23855 жыл бұрын

    Are you sure that the pI for glycine is 7 and not 5.97...🤔🤔 Isn't pI supposed to be calculated as (pKa1+pKa2)/2 For glycine it would be (2.34+9.60)/2=5.97

  • @Rohit.Kashab

    @Rohit.Kashab

    3 жыл бұрын

    exactly as we will get zwitterion in between these two ph's so he is wrong on this part

  • @nandu3110
    @nandu31105 жыл бұрын

    excellent

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @kavurinissy779
    @kavurinissy7796 жыл бұрын

    Sir pls make vdo on optical properties of aminoacids

  • @srujanasai2112
    @srujanasai21126 жыл бұрын

    can u please tell me about the selection rules

  • @kaitlynridgeway1405
    @kaitlynridgeway14052 жыл бұрын

    god bless your soul

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you

  • @Niksonics
    @Niksonics3 жыл бұрын

    Sir ionizable R grp wale..ka video hai?

  • @Kattiling
    @Kattiling2 жыл бұрын

    I don't get why the net charge of pH 12 is -1. Can someone explain why? I thought it would be -2, this is because there would be a negative charge from both the nitrogen and oxygen so (-1+-1=2). I don't understand why he ignored the charge in the oxygen in pH 12, but accounted the oxygen's charge in pH of 7.

  • @alejandrarodriguezcruz227
    @alejandrarodriguezcruz2273 жыл бұрын

    When KZread teaches you more than a top University :/

  • @nepalobro8788
    @nepalobro87884 жыл бұрын

    Thank you for nice video. What would be the net charge at pH 5.5? If the solution at pH 1 changed to pH 11, what would be net charge? and last, What would be net charge at pH 9.6 (pH equal to pka of NH2 grpup) ? It would help me in academic endeavour if I got response. Thank you again. :-)

  • @Biomaths247
    @Biomaths2476 жыл бұрын

    sir what will be the charge of glycine at pH 4,5,6,7,8, and 9. according to you will be zero.then what is PI value for glycine. it seems all of these ph values will be PI for glycine as well.

  • @adnanfaisal1085
    @adnanfaisal10852 жыл бұрын

    i have a question regarding pH 12. Amine already has no H+, so why did u say deprotonation and write -1 on top?

  • @20tejas
    @20tejas6 жыл бұрын

    please make a detailed video on thermodynamics of protein stability and vant hoffs equation

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    +20tejas okay. Will try to do that

  • @20tejas

    @20tejas

    6 жыл бұрын

    Shomu's Biology thank you.☺

  • @srikantadash4603

    @srikantadash4603

    4 жыл бұрын

    Plse sir

  • @mahaboobbashashaik5219
    @mahaboobbashashaik52193 жыл бұрын

    nice clarity

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @randhirkumarbt
    @randhirkumarbt3 жыл бұрын

    pls make a video to calculate the net charge and PI (isoelectric point) of a polypeptide at different pH. Problem N0.- 11 of Lehninger biochemistry, chapter -3, 4th edition

  • @termostat363
    @termostat3636 жыл бұрын

    thank you! god bless!

  • @swechhagyanvarsha5702
    @swechhagyanvarsha57023 жыл бұрын

    Can someone put in the link for next lecture?

  • @humairaniamat9349
    @humairaniamat93495 жыл бұрын

    Sir! please explain the titration curve of alanine

  • @arnavdeshpande3764
    @arnavdeshpande37643 жыл бұрын

    Thanks for the Video. Please check your calculations before uploading...

  • @RandomNooby
    @RandomNooby4 жыл бұрын

    @shomu Re COVID Why is the death rate in Italy in 0-18 year olds less than 2% with everyone else roughly evenly divided into 3 equal groups. With many young adults to 50 year olds suffering from a cytokine storm, what are your thoughts?

  • @yaramahmoud9926
    @yaramahmoud99265 жыл бұрын

    Thank you. That was very helpful.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @Zehra-9809
    @Zehra-98093 жыл бұрын

    Where is ur next class regarding this topic.

  • @treepotanime
    @treepotanime6 жыл бұрын

    any values in between 2.4 and 9.5 will be zero and how will we find the pi point?