Agarose gel electrophoresis
In this video we have described the practicals approach of the agarose gel electrophoresis. The agarose gel electrophoresis can be used to check the quality and quantity of the macromolecules such as DNA , RNA and proteins. Here in agarose gel electrophoresis the separation depends on charge and molecular weight and in some cases also depends on the conformation of the macromolecules. The agarose gel electrophoresis is routinely used for the analysis of PCR products, molecular markers, genomic DNA, Total RNA and proteins. In short the agarose gel electrophoresis is heart of the biotech and life science students who is working on the DNA, RNA and proteins.
Here we have explained the most of things and minute details but due to time limit and the topic is vast so we have tried our best to cover the topic …!!!!!!!! THANK YOU !
Credits
Video shot by - VInayak Mohandas 👌
Assisted by - Anusha Raj
Edited by -ME 🙈
Пікірлер: 103
Well explained.. Kudos to the team..😊👏
Nicely explained🙌🏻🙌🏻thank you sir
Very well explained 🙌
Thank you for useful information 🙏
Well explained .. 💯❤️
Thank u..This video helps me a lot...✨🙂
Chala baga explain chesav anna tq
Very informative video, keep it up, greetings from Germany!
very good brother , love from Pakistani ,Now Asia will rule ,West will follow .
@lifereplicon
2 жыл бұрын
Pls do share, it will encourage me to post more ! Thank you brother 😃
@abdulmueez4081
6 ай бұрын
💀?
Well done brother
Thank you sir.
Thank you so much.❤ Tomorrow is my biotechnology board practical 🙏🏻
@lifereplicon
6 ай бұрын
All the best 🙌
very good explanation
Now i understand ..thank you
@lifereplicon
2 жыл бұрын
Pls do share it will encourage me to post more ! Thank you 😃
Well explain bt background sound creats difficulties
Very much useful 👍👍 Pls upload more videos like this 🙌
@lifereplicon
2 жыл бұрын
Sure 😀
THank you ❤✌ You cleared all my doubts ⚡
@lifereplicon
2 жыл бұрын
Pls do share it will encourage me to post more ! Thank you 😃
🔥🔥
👍🏻👍🏻
👏👏
👌✌️👏👏❣️
Thank you for your explanation 🙏🏻✨
@lifereplicon
2 жыл бұрын
My pleasure 🥰
Dear, its well explained....good to use "Gel Red" rather than 'Ethidium bromide'. gel red is very safe.
@lifereplicon
7 ай бұрын
Thank you sir for your response in my previous lab i was using ETBR, currently we do use gel red only its very safe i agree and i do recommend !
Thank you
👍👍
👍👍👍
thank you sir so much... tomorrow i have board biotech practicals and you completely cleared all my doubts on how to do the experiment
@lifereplicon
6 ай бұрын
All the best 😉
superb... thank you 🙂
@lifereplicon
3 ай бұрын
Keep watching
🙌🙌
Thank you brother🙏kudos to you
@lifereplicon
2 жыл бұрын
Thank you 😊
Thank you ❤🙏
@lifereplicon
3 ай бұрын
Welcome!
Very nice explanation!
@lifereplicon
2 жыл бұрын
Thank you 😊 pls do share with ur friends family
Tq sir 🙏🏼🙏🏾
Well explained brother keep it up from Pakistan
@lifereplicon
2 жыл бұрын
Thank you dear 😊
well explained😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍. thank you
@lifereplicon
9 ай бұрын
Thank you 🙏
Its a good explanation sir... Keep up it up❤🎉
@lifereplicon
6 ай бұрын
Thanks a ton
Wow nice 😯😲
@lifereplicon
Жыл бұрын
Thank you
Sir I want to learn about crossed over gel electrophoresis
It's very well explained practical..
@lifereplicon
2 жыл бұрын
thank you pls do share with your friends ☺️
@sushmayadav3329
2 жыл бұрын
@@lifereplicon ya definitely.. I already done
@lifereplicon
2 жыл бұрын
Thank you 😊 really appreciated 😃
Very useful.... & i request u to kindly use a special microphone for yr clear voice. Thankyou 🙏🏼
@lifereplicon
Жыл бұрын
Thank you 😊 for suggestion i. Will implement it
Where is the gel image sir?
@lifereplicon
2 жыл бұрын
vyawahare.av@gmail.com pls dm so i can share
It was really nice.
Sir I need more videos which is imp in biotechnology field (techniques)
@lifereplicon
2 жыл бұрын
Sure 😃
Hi I'm having a doubt. How much 6x loading dye and DNA was added to the gel
@lifereplicon
2 жыл бұрын
Don't add to the gel 😐
✌✌
Very useful please upload SDS page also
@lifereplicon
2 жыл бұрын
I will upload
Will it mix properly by centrifuger? And can we use vortex for this? Will it break those dna or not?
@lifereplicon
7 ай бұрын
It will mix with brief centrifugation no need to vortex
Very nice explanation. BTW who are from 12th sciences
@lifereplicon
10 ай бұрын
Sorry I didn’t get you ?
@dhairyatrivedi2306
10 ай бұрын
Brother i am student of 12 th science and in biology we have DNA fingerprinting in which there is topic of gel electrophoresis
@lifereplicon
10 ай бұрын
@dhairyatrivedi2306 i hope u understood !
@dhairyatrivedi2306
10 ай бұрын
Yes thanx very nicely explained
Why two types of buffer used here ? If i use any one kind of buffer ( TBE/TAE) is any problem
@lifereplicon
Жыл бұрын
Not at all you can any buffer which is available for you Thank you
@microcolony6835
Жыл бұрын
@@lifereplicon ok sir
@microcolony6835
Жыл бұрын
Is TAE or TBE buffer is called running buffer?
@lifereplicon
11 ай бұрын
both are running buffers
How much ethidium bromide did u add to the gel?
@lifereplicon
Жыл бұрын
0.5ug/ml final conc
Bhai tum ye batao dye jb tum insert kr rhe to vo dikha na
@lifereplicon
3 ай бұрын
Sab dhikaya hai na sir
Every thing is very perfect but we can't hear your voice perfectly and moreover captions are not available so please make sure that your voice is clear to understand your important information sir
@lifereplicon
Жыл бұрын
Thank you i was on low budget during that time
@venkateshjeedimetla1471
Жыл бұрын
@@lifereplicon may God solved your budget problem now and wishing you to rock amazingly in KZread
@lifereplicon
Жыл бұрын
Pls do like s SHARE and subscribe ! I need support
Good but voice is not clear
@lifereplicon
Жыл бұрын
I will improve thank you for suggestions
etbr is added to buffer?
@lifereplicon
2 жыл бұрын
No its added to melted gel
@riya8910
2 жыл бұрын
@@liferepliconin well?
@lifereplicon
2 жыл бұрын
No in gel
@riya8910
2 жыл бұрын
It can be added , after whole process is done ...? For just visualisation
@lifereplicon
2 жыл бұрын
No it should be mixed throughout the gel as the DNA or RNA. Can be visualise at anywhere in the gel
JUST IMPROVE VOICE QUALITY REST U R BEST
@lifereplicon
Жыл бұрын
Thank you