3D Cell Culture Scale-Up with the PBS-MINI Bioreactor
Ғылым және технология
Learn how the PBS-MINI Bioreactor can be used to scale up 3D cell cultures. Its gentle yet efficient mixing allows you to achieve a high volume of cell aggregates in suspension while maintaining high cell viability for various downstream applications.
To learn more, visit www.stemcell.com/PBS-MINI.
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Пікірлер: 2
Does it applicable for adherent cells?
@STEMCELLTechnologies
11 ай бұрын
Adherent hPSC 2D cultures grown on Corning® Matrigel® with mTeSR™1, mTeSR™ Plus, TeSR™-AOF or TeSR™-E8™ can be transitioned directly into dynamic suspension culture. To successfully expand cells and maintain pluripotency in a suspension culture system, it is crucial to begin with high-quality hPSC cultures. Typically, hPSCs are maintained in 2D on Corning® Matrigel® with mTeSR™1, mTeSR™ Plus, TeSR™-AOF, or TeSR™-E8™, and are passaged with Gentle Cell Dissociation Reagent (Catalog #100-0485) as clumps that are 50 - 200 µm in diameter. For 3D cultures, clumps from 2D cultures are first cultured in 6-well plates on a shaker, then in bottles, and can then be upscaled further to the PBS Mini. For more detailed information, please read this technical tip: www.stemcell.com/robust-scale-up-of-human-pluripotent-stem-cells-using-tesr-3d-media.html. If you have any other questions, feel free to email us at techsupport@stemcell.com.