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In this webinar, Aurélie Tchoghandjian, PhD, presents how her team uses syngeneic mouse and patient-derived models to investigate the influence of Inhibitor of apoptosis proteins (IAP) on phenotype, spatio-temporal distribution, and function of tumor-associated macrophages (TAMs) in glioblastoma. Learn how they combined deep immunophenotyping, light sheet 3D imaging, functional assays, and single-cell RNA sequencing to discover that IAP inhibition can reprogram TAMs, promote TAMs infiltration into the glioblastoma microenvironment, and remodel the tumoral vasculature. This better knowledge of spatial distribution and function of TAMs is the first step to restoring an immunocompetent glioblastoma environment and thereby increasing treatment efficiency.
The tumor microenvironment (TME) possesses complex cellular and structural features. Understanding the interactions that occur between the different components within the TME are essential for identifying mechanisms that drive cancer development and therapy escape, and for establishing strategies to tackle these. Miltenyi Biotec developed various workflows for TME analysis even from limited sample material. These tools get you a 360° view - from characterizing single cells to visualizing their natural spatial context. They help you identify cell interactions within the TME and understand resulting phenotypes to unravel tumor complexity.