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  • @wafaamaged3570
    @wafaamaged35705 күн бұрын

    Good video

  • @simranshah3478
    @simranshah34783 ай бұрын

    Wow!! Amazed. Thank you

  • @falotfalco
    @falotfalco4 ай бұрын

    you take a piece of tissue. you first flood it with chemicals. then you dry it. you freeze and I don't know what else... and then you prove viruses in such a sample? don't fuck. when you get the whole procedure through, then the pattern is no longer the same as it was. but if you doused it with chemicals, dehydrated it, froze it and then finally baked it with electrons at 150 degrees cesium.

  • @ahmadsalim1569
    @ahmadsalim15699 ай бұрын

    Thank you so much it was really helpful. Keep up the good work

  • @megraelynsatterthwaite
    @megraelynsatterthwaite11 ай бұрын

    do you really think this research reflects biological reality after all this processing?

  • @II-th7bn
    @II-th7bn4 ай бұрын

    Nice question

  • @minalbhalerao
    @minalbhalerao Жыл бұрын

    Very useful video of ultramicrotomy sample preparation and processing. Thank you very much.

  • @krishnappa3982
    @krishnappa3982 Жыл бұрын

    10:03 What is name of blue color container having water and knife in it?

  • @krishnappa3982
    @krishnappa3982 Жыл бұрын

    What is that drop in which you are finally dropping the grid?

  • @as91as1
    @as91as1 Жыл бұрын

    very helpful thank you so much

  • @dimaxxi1426
    @dimaxxi1426 Жыл бұрын

    يعطيكم العافية

  • @UmaMaheswari-jq4dq
    @UmaMaheswari-jq4dq Жыл бұрын

    Thanks a lot

  • @treefafaroqismail6222
    @treefafaroqismail6222 Жыл бұрын

    thank you for sharing very clear and interesting explanation

  • @tanzimmaruf2194
    @tanzimmaruf2194 Жыл бұрын

    Nice and standard

  • @sabafj369
    @sabafj3692 жыл бұрын

    Some of the steps the voice was cut. How much time needed to keep the sample with the stain? Same thing with the last step.

  • @karenbernaba6793
    @karenbernaba67932 жыл бұрын

    wow you saveddddd my life! best video

  • @uroosamanal8356
    @uroosamanal83562 жыл бұрын

    Thank you mam🌺 Very Interesting and Amazing ☺👍

  • @factsfirm6672
    @factsfirm66722 жыл бұрын

    Why do we add uranyl acetate first and then lead citrate?

  • @tariqkhanPathanKabuliwala
    @tariqkhanPathanKabuliwala2 жыл бұрын

    Excellent, very complicated. Thanks for sharing, enlightening us.

  • @rudraprasad8934
    @rudraprasad89342 жыл бұрын

    Very well explained Thank you for your hardwork

  • @galactusgamerzzz2104
    @galactusgamerzzz21042 жыл бұрын

    Thank you madam

  • @rabeaaslam3968
    @rabeaaslam39682 жыл бұрын

    Fixation by glutaraldehdye Wash with optimum tetraoxide Wash with pbs Dehydration with ethanol Propylene oxide Infiltrate with resin Embedding put resin inside a mold Oven for overnight 60 degrees Cut the plastic mold Sectioning by ultramicrotome

  • @exploringworldbeauty
    @exploringworldbeauty2 жыл бұрын

    how long sample need to put osmium tetraoxide ? after stain with lead citrate then the sample can be taken for electron microscope ?

  • @sushilbarua8862
    @sushilbarua88622 жыл бұрын

    Please see tobbaco mosaic virus and coronavirus in microscope like smallpox

  • @aliya9711
    @aliya97112 жыл бұрын

    how much time the sample should be incubated with Osmium Tetroxide

  • @amiraanwar6095
    @amiraanwar60952 жыл бұрын

    thanks

  • @anjusangwan4217
    @anjusangwan42172 жыл бұрын

    What was the resin name? please mention

  • @uroosamanal8356
    @uroosamanal83562 жыл бұрын

    Epoxy Resin....

  • @Manisha_Gusain99
    @Manisha_Gusain993 жыл бұрын

    Very much intresting 👏👏👏

  • 3 жыл бұрын

    It's more complicated than I thought :D Thank you.

  • @MuhammadHussain-fu9hk
    @MuhammadHussain-fu9hk3 жыл бұрын

    Please your sound is not clear

  • @uproarmatrix1745
    @uproarmatrix17452 жыл бұрын

    🤡

  • @MuhammadHussain-fu9hk
    @MuhammadHussain-fu9hk3 жыл бұрын

    So good

  • @paramedical8133
    @paramedical81333 жыл бұрын

    60 degree temp incubation????

  • @Sk-tq7ih
    @Sk-tq7ih3 жыл бұрын

    Thanks

  • @srikantadash4603
    @srikantadash46033 жыл бұрын

    😘😌o god 🙏.... Awesome yar... It's just amazing.

  • @alannolan3514
    @alannolan35143 жыл бұрын

    You deserve a raise!

  • @samahalhorani5566
    @samahalhorani55663 жыл бұрын

    Can be applied for TiO2 sample preparation

  • @montrayjones1947
    @montrayjones19474 жыл бұрын

    Thanks for this video! Helped me with my biochemistry final.

  • @ezinneimoko1898
    @ezinneimoko18984 жыл бұрын

    Please what is the first staining reagent?? I heard urine acetate 🤔

  • @fasfas5405
    @fasfas54054 жыл бұрын

    Uranyl acetate

  • @archanamohandoss9975
    @archanamohandoss99758 күн бұрын

    😂

  • @Jozn3
    @Jozn34 жыл бұрын

    Some tips at sectioning. Remove the spec. trans light cable. Turn the Chuck not the hole Seg.Arc. and use a Trimmer with a diamond cutter. The razor blades causes defects in your diatome knife sooner or later. :-)

  • @canaltres5932
    @canaltres59324 жыл бұрын

    Oddly satisfying <3 <3

  • @alejandratellez8848
    @alejandratellez88484 жыл бұрын

    “We are now sectioning our sample *machine beeps* oh god dang it” I felt that

  • @ayeshairshad8681
    @ayeshairshad86813 жыл бұрын

    can you tell me what was the specimen used in this prepration

  • @alejandratellez8848
    @alejandratellez88483 жыл бұрын

    @@ayeshairshad8681 no, they never mentioned what sample it is but my guess is tissue but looks more like plant

  • @ayeshairshad8681
    @ayeshairshad86813 жыл бұрын

    @@alejandratellez8848 ok thanks

  • @anjusangwan4217
    @anjusangwan42172 жыл бұрын

    can you tell me which resin to use for post embedding of plant samples? mention resin name please

  • @mistikaalmira7174
    @mistikaalmira71744 жыл бұрын

    How do you prepare red blood cells for SEM?

  • @ankitayadav1123
    @ankitayadav11234 жыл бұрын

    Thank you Ma'am for this clear explanation 😀

  • @1geniusme
    @1geniusme4 жыл бұрын

    Too complicated after 14:45, pls explain further.

  • @tamaraali109
    @tamaraali1094 жыл бұрын

    لم افهم اي شيء

  • @tamaraali109
    @tamaraali1094 жыл бұрын

    محاضرة قيمة ودكتور رائع بالتوفيق لطلابه⁦👍🏻⁩

  • @dertejavi1
    @dertejavi15 жыл бұрын

    hi i have a question, first at all dorry for my english!. to discard the osmiun u use a container onli for it? or u mix with the another discards? thx