you take a piece of tissue. you first flood it with chemicals. then you dry it. you freeze and I don't know what else... and then you prove viruses in such a sample? don't fuck. when you get the whole procedure through, then the pattern is no longer the same as it was. but if you doused it with chemicals, dehydrated it, froze it and then finally baked it with electrons at 150 degrees cesium.
@ahmadsalim15699 ай бұрын
Thank you so much it was really helpful. Keep up the good work
@megraelynsatterthwaite11 ай бұрын
do you really think this research reflects biological reality after all this processing?
@II-th7bn4 ай бұрын
Nice question
@minalbhalerao Жыл бұрын
Very useful video of ultramicrotomy sample preparation and processing. Thank you very much.
@krishnappa3982 Жыл бұрын
10:03 What is name of blue color container having water and knife in it?
@krishnappa3982 Жыл бұрын
What is that drop in which you are finally dropping the grid?
@as91as1 Жыл бұрын
very helpful thank you so much
@dimaxxi1426 Жыл бұрын
يعطيكم العافية
@UmaMaheswari-jq4dq Жыл бұрын
Thanks a lot
@treefafaroqismail6222 Жыл бұрын
thank you for sharing very clear and interesting explanation
@tanzimmaruf2194 Жыл бұрын
Nice and standard
@sabafj3692 жыл бұрын
Some of the steps the voice was cut. How much time needed to keep the sample with the stain? Same thing with the last step.
@karenbernaba67932 жыл бұрын
wow you saveddddd my life! best video
@uroosamanal83562 жыл бұрын
Thank you mam🌺 Very Interesting and Amazing ☺👍
@factsfirm66722 жыл бұрын
Why do we add uranyl acetate first and then lead citrate?
@tariqkhanPathanKabuliwala2 жыл бұрын
Excellent, very complicated. Thanks for sharing, enlightening us.
@rudraprasad89342 жыл бұрын
Very well explained Thank you for your hardwork
@galactusgamerzzz21042 жыл бұрын
Thank you madam
@rabeaaslam39682 жыл бұрын
Fixation by glutaraldehdye Wash with optimum tetraoxide Wash with pbs Dehydration with ethanol Propylene oxide Infiltrate with resin Embedding put resin inside a mold Oven for overnight 60 degrees Cut the plastic mold Sectioning by ultramicrotome
@exploringworldbeauty2 жыл бұрын
how long sample need to put osmium tetraoxide ? after stain with lead citrate then the sample can be taken for electron microscope ?
@sushilbarua88622 жыл бұрын
Please see tobbaco mosaic virus and coronavirus in microscope like smallpox
@aliya97112 жыл бұрын
how much time the sample should be incubated with Osmium Tetroxide
@amiraanwar60952 жыл бұрын
thanks
@anjusangwan42172 жыл бұрын
What was the resin name? please mention
@uroosamanal83562 жыл бұрын
Epoxy Resin....
@Manisha_Gusain993 жыл бұрын
Very much intresting 👏👏👏
3 жыл бұрын
It's more complicated than I thought :D Thank you.
@MuhammadHussain-fu9hk3 жыл бұрын
Please your sound is not clear
@uproarmatrix17452 жыл бұрын
🤡
@MuhammadHussain-fu9hk3 жыл бұрын
So good
@paramedical81333 жыл бұрын
60 degree temp incubation????
@Sk-tq7ih3 жыл бұрын
Thanks
@srikantadash46033 жыл бұрын
😘😌o god 🙏.... Awesome yar... It's just amazing.
@alannolan35143 жыл бұрын
You deserve a raise!
@samahalhorani55663 жыл бұрын
Can be applied for TiO2 sample preparation
@montrayjones19474 жыл бұрын
Thanks for this video! Helped me with my biochemistry final.
@ezinneimoko18984 жыл бұрын
Please what is the first staining reagent?? I heard urine acetate 🤔
@fasfas54054 жыл бұрын
Uranyl acetate
@archanamohandoss99758 күн бұрын
😂
@Jozn34 жыл бұрын
Some tips at sectioning. Remove the spec. trans light cable. Turn the Chuck not the hole Seg.Arc. and use a Trimmer with a diamond cutter. The razor blades causes defects in your diatome knife sooner or later. :-)
@canaltres59324 жыл бұрын
Oddly satisfying <3 <3
@alejandratellez88484 жыл бұрын
“We are now sectioning our sample *machine beeps* oh god dang it” I felt that
@ayeshairshad86813 жыл бұрын
can you tell me what was the specimen used in this prepration
@alejandratellez88483 жыл бұрын
@@ayeshairshad8681 no, they never mentioned what sample it is but my guess is tissue but looks more like plant
@ayeshairshad86813 жыл бұрын
@@alejandratellez8848 ok thanks
@anjusangwan42172 жыл бұрын
can you tell me which resin to use for post embedding of plant samples? mention resin name please
@mistikaalmira71744 жыл бұрын
How do you prepare red blood cells for SEM?
@ankitayadav11234 жыл бұрын
Thank you Ma'am for this clear explanation 😀
@1geniusme4 жыл бұрын
Too complicated after 14:45, pls explain further.
@tamaraali1094 жыл бұрын
لم افهم اي شيء
@tamaraali1094 жыл бұрын
محاضرة قيمة ودكتور رائع بالتوفيق لطلابه👍🏻
@dertejavi15 жыл бұрын
hi i have a question, first at all dorry for my english!. to discard the osmiun u use a container onli for it? or u mix with the another discards? thx
Пікірлер
Good video
Wow!! Amazed. Thank you
you take a piece of tissue. you first flood it with chemicals. then you dry it. you freeze and I don't know what else... and then you prove viruses in such a sample? don't fuck. when you get the whole procedure through, then the pattern is no longer the same as it was. but if you doused it with chemicals, dehydrated it, froze it and then finally baked it with electrons at 150 degrees cesium.
Thank you so much it was really helpful. Keep up the good work
do you really think this research reflects biological reality after all this processing?
Nice question
Very useful video of ultramicrotomy sample preparation and processing. Thank you very much.
10:03 What is name of blue color container having water and knife in it?
What is that drop in which you are finally dropping the grid?
very helpful thank you so much
يعطيكم العافية
Thanks a lot
thank you for sharing very clear and interesting explanation
Nice and standard
Some of the steps the voice was cut. How much time needed to keep the sample with the stain? Same thing with the last step.
wow you saveddddd my life! best video
Thank you mam🌺 Very Interesting and Amazing ☺👍
Why do we add uranyl acetate first and then lead citrate?
Excellent, very complicated. Thanks for sharing, enlightening us.
Very well explained Thank you for your hardwork
Thank you madam
Fixation by glutaraldehdye Wash with optimum tetraoxide Wash with pbs Dehydration with ethanol Propylene oxide Infiltrate with resin Embedding put resin inside a mold Oven for overnight 60 degrees Cut the plastic mold Sectioning by ultramicrotome
how long sample need to put osmium tetraoxide ? after stain with lead citrate then the sample can be taken for electron microscope ?
Please see tobbaco mosaic virus and coronavirus in microscope like smallpox
how much time the sample should be incubated with Osmium Tetroxide
thanks
What was the resin name? please mention
Epoxy Resin....
Very much intresting 👏👏👏
It's more complicated than I thought :D Thank you.
Please your sound is not clear
🤡
So good
60 degree temp incubation????
Thanks
😘😌o god 🙏.... Awesome yar... It's just amazing.
You deserve a raise!
Can be applied for TiO2 sample preparation
Thanks for this video! Helped me with my biochemistry final.
Please what is the first staining reagent?? I heard urine acetate 🤔
Uranyl acetate
😂
Some tips at sectioning. Remove the spec. trans light cable. Turn the Chuck not the hole Seg.Arc. and use a Trimmer with a diamond cutter. The razor blades causes defects in your diatome knife sooner or later. :-)
Oddly satisfying <3 <3
“We are now sectioning our sample *machine beeps* oh god dang it” I felt that
can you tell me what was the specimen used in this prepration
@@ayeshairshad8681 no, they never mentioned what sample it is but my guess is tissue but looks more like plant
@@alejandratellez8848 ok thanks
can you tell me which resin to use for post embedding of plant samples? mention resin name please
How do you prepare red blood cells for SEM?
Thank you Ma'am for this clear explanation 😀
Too complicated after 14:45, pls explain further.
لم افهم اي شيء
محاضرة قيمة ودكتور رائع بالتوفيق لطلابه👍🏻
hi i have a question, first at all dorry for my english!. to discard the osmiun u use a container onli for it? or u mix with the another discards? thx