Me gustaria que me ayudaran. Tengo el mismo modelo de microtomo y últimamente cuando estoy sacando la tira siento que el microtomo da pequeño salto entonces sale un fino y uno grueso uno fino y uno grueso. Por.lo regular corto en 4 micra y la.grasa en 5. Revise el microtomo y al.parecer esta calibrado. Que puede ser?
@hannahanhan27293 ай бұрын
Hi, I have a question. Which block mailer did you use? do you have the link where you buy it from? I couldn't find the correct block mailer to hold my samples into the microtome machine, the ones I found online were too big.
@coop532910 ай бұрын
Well, it's not how I did it, but I guess different strokes for different folks. Too slow, too thick, too likely to cut yourself, etc. etc.
@Talukdar_Vlog11 ай бұрын
Thank you so much! Learned a lot.
@StephenWambuaKen Жыл бұрын
Bien. Wonderful
@diannebee Жыл бұрын
What in the world are you doing!?
@yorkrojas1452 Жыл бұрын
Amazing
@user-br1sz8cc7p Жыл бұрын
wow great video! ❤
@vd8787 Жыл бұрын
dédicace à la fac de poitiers, tous les shegueys de la nezo aie aie aie on va l'avoir notre l3 les frr
@musawirjaved786 Жыл бұрын
edical Laboratory Technician www.testlabpk.com/ What Is Malaria www.testlabpk.com/2022/08/what-is-malaria.html TYPES OF SEPECIMENS www.testlabpk.com/2022/08/typs-of-sepecimens-1-we-can-get.html ANTIBODY ,IgG,IgM,IgA,IgE,IgD www.testlabpk.com/2022/08/antibody-iggigmigaigeigd.html GRAM STAIN & ZIEHL- NEELSEN STAIN www.testlabpk.com/2022/08/gram-stain-ziehl-neelsen-stain.html Smear preparation and fixation www.testlabpk.com/2022/08/smear-preparation-and-fixation.html Erythroocyte Sedimentation Rate (ESR) www.testlabpk.com/2022/08/erythrocyte-sedimentation-rate.html
@nsbsbbdbdhd71462 жыл бұрын
O God pray on Mohammed and the family of Mohammed the pure good and hurry their release Oh generous
@piyamongi26582 жыл бұрын
Amazing machine for cutting sections #saraikiLearning
@user-tt8xy7js4f2 жыл бұрын
عاشت ايدك👍🏻
@dr.devendrasaran70663 жыл бұрын
Paraffin block's kept in the water for 24 Hrs. Using of water is cold or normal water??
@zolo4600 Жыл бұрын
but why
@JoseOrtiz-tf9vy3 жыл бұрын
"put the block in water, after 24 hrs put the block back..." lol homie I got to finish sectioning so I can go home
@ohkei25213 жыл бұрын
Same 😂
@ayubali97922 жыл бұрын
Never heard of that before.. a couple mins should do the job
@samersalem99513 жыл бұрын
Why you put the block in the water and waiting 24hourse
@depressed74014 жыл бұрын
this is good ❤️💁🏻♀️
@user-gw8ru5rf3b4 жыл бұрын
Работягам из 304 группы привет, остальным соболезную...
@kawthardrf86734 жыл бұрын
Thank you 😊
@maheshwarareddyjedla69914 жыл бұрын
sir, can we use this for cutting metal sheets??
@coop532910 ай бұрын
NO. duh
@tanukashyap14395 жыл бұрын
good Info
@ajaygautam13335 жыл бұрын
So nice
@ethernicagape31635 жыл бұрын
why do you cut wax from the block??? it will be removed before colorations anyway...
@radiabenzerdjeb43746 жыл бұрын
Slt, merci pour la vidéo mais j'aurais bien aimer voir la suite question pour fait fait une comparaison ! Moi je fais l'étalement avec un liquide qui est un mélange d'albumine et glycérine et eau, j'ai pas mal de fois essaye la méthode du bain marie mais après coloration les fragments se décollent , g hâte de savoir comment vous faites le deparaffinage après étalement et la coloration que vous utilisez, merci infiniment de me répondre !
@humanism10416 жыл бұрын
good
@towens11686 жыл бұрын
I don't want to be mean but please dear god if you are new to histology DO NOT use this video as a tutorial!!
@diatomsaus4 жыл бұрын
What's a better guide or source for beginners? Reading the several comments here got me scared.
@towens11684 жыл бұрын
@@diatomsaus So I haven't checked out any histo videos on youtube in forever because there just wasn't anything good but that could have changed by now. The best way to learn is to watch experienced people that you work with and ask questions. Honestly microtomy is about practice and muscle memory. if you are having specific issues check out ihcworld.com's troubleshooting guide. Just google microtome sectioning troubleshooting.
@diatomsaus4 жыл бұрын
@@towens1168 Oh wow, thanks for the information! I didn't expect a response on a 2-year-old comment. Nice suggestions there. The lab I work at doesn't deal with sectioning at all, there's a biology one that I don't have access to, they do all the sectioning. I guess I'll try and somehow wiggle in to learn stuff. I'm looking to grow my personal "lab" for microscopy imaging rather than anything professional. Sometimes, these manual microtomes pop up on flea market sites at alright prices.
@towens11684 жыл бұрын
@@diatomsaus Yeah no problem. So if you're going to be using already prepared blocks that might work. You will still need to buy the chemicals to H&E stain the slides at the very least. I don't think Hematoxylin or Eosin are controlled chemicals so you should be able to get some if you want (I could be wrong about that never looked them up). If you want to prepare your own blocks that gets much more complicated. You need to fix the tissue first and then process it and then you need to be able to embed it. I imagine that could all be done without a processor or an embedding station but it would be a pain at best. Processing takes anywhere from a few hours to overnight. Honestly there's a lot of work and money (and skill) that goes into making a slide. If the imaging is what you're really interested in you might be better off just buying slides.
@diatomsaus4 жыл бұрын
@@towens1168 Yeah, at the moment I have a big collection of slides. Some vintage, some from preparers such as Klaus Kemp who deals with diatoms, recently (sadly) retired. Out of all the Chinese ones I've tried, I found one university ran company that offers great quality at prices that are a lot higher (similar prices to western companies), but quality is what I'm after so it's fine. Generally, Carolina(dot)com offers exceptional slides to anyone who wants to buy prepared ones, their shipping costs isn't bad either. I've already spent enough money on all the microscope stuff. I've decided to forget about making my own slides unless I get the chances to do so at work (our labs have pretty liberal policies, we can visit for our own projects luckily). A microtome is anywhere from $500-$1000+ with blades being another expensive disposable, along with all the staining chemicals and issues with dust/fibres which sort of warrants a benchtop clean-room assembly cabinet... it's an endless hole. Either way, thanks for your insight!
@Bjr8787876 жыл бұрын
That’s the most ridiculous technique for floating ribbon. Why wouldn’t you just anchor your ribbon the one side of your water bath. Who wants to chase the ribbon all over their water like that? You must work in a very low volume lab to be soaking your blocks for one to twenty four hours. That’s insane!!! Who has processors that are drying the tissue out that much they need soaking for hours?
@mgillett6 жыл бұрын
Wow. 1. Who sections paraffin normally at 10um? 2. You soak the block on the chuck. 3. Use a god damn brush to align the sections on the slide! Does no one look at histotech manual (Armed Forces) anymore?
@jp54026 жыл бұрын
I don't even know where to begin! There were so many mistakes here its not even worth trying to call them all out. I'm sure there are some good videos but every one I have seen on KZread sucks. Will someone actually good at there job make a video on a sectioning. This is so cringe worthy!
@melaniee4675 жыл бұрын
Please go and do your own video, I'm curious and want to learn
@tabitameydia77297 жыл бұрын
doesnt it waste more time?
@moatazalasad47517 жыл бұрын
good
@cacacenazo7 жыл бұрын
Thank you very much! Very useful.
@rajeshsahu14567 жыл бұрын
hey nice cuting thanks
@giselleluna10547 жыл бұрын
que modelo es el microtomo?
@celtclan7 жыл бұрын
OMG. You do not put your fingers in the water bath or pick up sections like that.
@camman10567 жыл бұрын
Instructions not clear enough, ended up sectioning my dick. Got a good mark for technique though
@viktorkharazia65537 жыл бұрын
please always, ALWAYS!!! cover the blade (and lock the flywheel) when bring your fingers near the blade - Histotech Instructor
@sarmadghafoor148421 күн бұрын
if you do that though now days you will be called slow ext ext
@moskaarya75047 жыл бұрын
Thank you for the video as I am still a student in histology but from what I have been thought you must always have the knife guard on if not in use.
@victoreke85512 жыл бұрын
Ik it's been 5years but how has things been for you
@evilania738 жыл бұрын
I´ve never heard about soaking paraffin embedded tissue samples for 24 hours in ice water...I´m in histology for 20 years now....also it is very dangerous trimming a paraffinblock over a sharp knife without gloves.....
@melaniee4675 жыл бұрын
I do put the blocks while trimming in ice water when its hot, it prevents from "melting" a bit
@majidamaji18824 жыл бұрын
@@melaniee467 how to trim...?
@vijishavijayakumar50658 жыл бұрын
very useful.. thank you...
@boscoitalics8 жыл бұрын
hope you fixed her :( probably a ear infection
@binuwannawagamuwa80298 жыл бұрын
This video absolutely gives an knowledge about the microtome cutter too...........
@matadorabullfighter31318 жыл бұрын
Wear gloves! That is lab specimen processing 101! Universal Precautions is a must when handling blood and tissues.
@mgbon28 жыл бұрын
It's fixed, chill...
@mgbon28 жыл бұрын
If anything, wear gloves so you don't contaminate your sections but only a few cowboys use their fingers in the water bath
@cafinario5 жыл бұрын
He is handling fixed, dehydrated, xylene-treated, embedded blocks. He is not handling blood or fresh tissues in the video. Stop the safety paranoia.
@TheyDontKnowUsAtAll8 жыл бұрын
Thank you!
@xksingo8 жыл бұрын
Just FYI peeps. Certain tissue types need more hydration ( hence the variance in soaking times). If a tissue is dry for sectioning such as brain or liver, it needs to sit on wet ice for longer. If tissue is fatty such as deep melanomas, breast, lipomas etc soaking the block on water is about the biggest mistake you can make. It will interfere with sectioning if you introduce water after processing. It will be incompatible with xylene that is still present in the tissue and explode on the water bath. The whole point of soaking your block prior to sectioning is to hydrate the tissue so that the morphology is more accurate on the slide.
@mgbon28 жыл бұрын
I have been taught to keep blocks on ice after trimming. Sections just fall off at 4microns
@waleedalghanmi49668 жыл бұрын
good thank you
@obudhojames69378 жыл бұрын
nice
@pradipneupane53128 жыл бұрын
very good
@millatkurdimedical53258 жыл бұрын
Very Good ...
@xksingo8 жыл бұрын
What type of tissue are u sectioning that you soak for 24 hrs? Is this for special studies or routine staining?
Пікірлер
Me gustaria que me ayudaran. Tengo el mismo modelo de microtomo y últimamente cuando estoy sacando la tira siento que el microtomo da pequeño salto entonces sale un fino y uno grueso uno fino y uno grueso. Por.lo regular corto en 4 micra y la.grasa en 5. Revise el microtomo y al.parecer esta calibrado. Que puede ser?
Hi, I have a question. Which block mailer did you use? do you have the link where you buy it from? I couldn't find the correct block mailer to hold my samples into the microtome machine, the ones I found online were too big.
Well, it's not how I did it, but I guess different strokes for different folks. Too slow, too thick, too likely to cut yourself, etc. etc.
Thank you so much! Learned a lot.
Bien. Wonderful
What in the world are you doing!?
Amazing
wow great video! ❤
dédicace à la fac de poitiers, tous les shegueys de la nezo aie aie aie on va l'avoir notre l3 les frr
edical Laboratory Technician www.testlabpk.com/ What Is Malaria www.testlabpk.com/2022/08/what-is-malaria.html TYPES OF SEPECIMENS www.testlabpk.com/2022/08/typs-of-sepecimens-1-we-can-get.html ANTIBODY ,IgG,IgM,IgA,IgE,IgD www.testlabpk.com/2022/08/antibody-iggigmigaigeigd.html GRAM STAIN & ZIEHL- NEELSEN STAIN www.testlabpk.com/2022/08/gram-stain-ziehl-neelsen-stain.html Smear preparation and fixation www.testlabpk.com/2022/08/smear-preparation-and-fixation.html Erythroocyte Sedimentation Rate (ESR) www.testlabpk.com/2022/08/erythrocyte-sedimentation-rate.html
O God pray on Mohammed and the family of Mohammed the pure good and hurry their release Oh generous
Amazing machine for cutting sections #saraikiLearning
عاشت ايدك👍🏻
Paraffin block's kept in the water for 24 Hrs. Using of water is cold or normal water??
but why
"put the block in water, after 24 hrs put the block back..." lol homie I got to finish sectioning so I can go home
Same 😂
Never heard of that before.. a couple mins should do the job
Why you put the block in the water and waiting 24hourse
this is good ❤️💁🏻♀️
Работягам из 304 группы привет, остальным соболезную...
Thank you 😊
sir, can we use this for cutting metal sheets??
NO. duh
good Info
So nice
why do you cut wax from the block??? it will be removed before colorations anyway...
Slt, merci pour la vidéo mais j'aurais bien aimer voir la suite question pour fait fait une comparaison ! Moi je fais l'étalement avec un liquide qui est un mélange d'albumine et glycérine et eau, j'ai pas mal de fois essaye la méthode du bain marie mais après coloration les fragments se décollent , g hâte de savoir comment vous faites le deparaffinage après étalement et la coloration que vous utilisez, merci infiniment de me répondre !
good
I don't want to be mean but please dear god if you are new to histology DO NOT use this video as a tutorial!!
What's a better guide or source for beginners? Reading the several comments here got me scared.
@@diatomsaus So I haven't checked out any histo videos on youtube in forever because there just wasn't anything good but that could have changed by now. The best way to learn is to watch experienced people that you work with and ask questions. Honestly microtomy is about practice and muscle memory. if you are having specific issues check out ihcworld.com's troubleshooting guide. Just google microtome sectioning troubleshooting.
@@towens1168 Oh wow, thanks for the information! I didn't expect a response on a 2-year-old comment. Nice suggestions there. The lab I work at doesn't deal with sectioning at all, there's a biology one that I don't have access to, they do all the sectioning. I guess I'll try and somehow wiggle in to learn stuff. I'm looking to grow my personal "lab" for microscopy imaging rather than anything professional. Sometimes, these manual microtomes pop up on flea market sites at alright prices.
@@diatomsaus Yeah no problem. So if you're going to be using already prepared blocks that might work. You will still need to buy the chemicals to H&E stain the slides at the very least. I don't think Hematoxylin or Eosin are controlled chemicals so you should be able to get some if you want (I could be wrong about that never looked them up). If you want to prepare your own blocks that gets much more complicated. You need to fix the tissue first and then process it and then you need to be able to embed it. I imagine that could all be done without a processor or an embedding station but it would be a pain at best. Processing takes anywhere from a few hours to overnight. Honestly there's a lot of work and money (and skill) that goes into making a slide. If the imaging is what you're really interested in you might be better off just buying slides.
@@towens1168 Yeah, at the moment I have a big collection of slides. Some vintage, some from preparers such as Klaus Kemp who deals with diatoms, recently (sadly) retired. Out of all the Chinese ones I've tried, I found one university ran company that offers great quality at prices that are a lot higher (similar prices to western companies), but quality is what I'm after so it's fine. Generally, Carolina(dot)com offers exceptional slides to anyone who wants to buy prepared ones, their shipping costs isn't bad either. I've already spent enough money on all the microscope stuff. I've decided to forget about making my own slides unless I get the chances to do so at work (our labs have pretty liberal policies, we can visit for our own projects luckily). A microtome is anywhere from $500-$1000+ with blades being another expensive disposable, along with all the staining chemicals and issues with dust/fibres which sort of warrants a benchtop clean-room assembly cabinet... it's an endless hole. Either way, thanks for your insight!
That’s the most ridiculous technique for floating ribbon. Why wouldn’t you just anchor your ribbon the one side of your water bath. Who wants to chase the ribbon all over their water like that? You must work in a very low volume lab to be soaking your blocks for one to twenty four hours. That’s insane!!! Who has processors that are drying the tissue out that much they need soaking for hours?
Wow. 1. Who sections paraffin normally at 10um? 2. You soak the block on the chuck. 3. Use a god damn brush to align the sections on the slide! Does no one look at histotech manual (Armed Forces) anymore?
I don't even know where to begin! There were so many mistakes here its not even worth trying to call them all out. I'm sure there are some good videos but every one I have seen on KZread sucks. Will someone actually good at there job make a video on a sectioning. This is so cringe worthy!
Please go and do your own video, I'm curious and want to learn
doesnt it waste more time?
good
Thank you very much! Very useful.
hey nice cuting thanks
que modelo es el microtomo?
OMG. You do not put your fingers in the water bath or pick up sections like that.
Instructions not clear enough, ended up sectioning my dick. Got a good mark for technique though
please always, ALWAYS!!! cover the blade (and lock the flywheel) when bring your fingers near the blade - Histotech Instructor
if you do that though now days you will be called slow ext ext
Thank you for the video as I am still a student in histology but from what I have been thought you must always have the knife guard on if not in use.
Ik it's been 5years but how has things been for you
I´ve never heard about soaking paraffin embedded tissue samples for 24 hours in ice water...I´m in histology for 20 years now....also it is very dangerous trimming a paraffinblock over a sharp knife without gloves.....
I do put the blocks while trimming in ice water when its hot, it prevents from "melting" a bit
@@melaniee467 how to trim...?
very useful.. thank you...
hope you fixed her :( probably a ear infection
This video absolutely gives an knowledge about the microtome cutter too...........
Wear gloves! That is lab specimen processing 101! Universal Precautions is a must when handling blood and tissues.
It's fixed, chill...
If anything, wear gloves so you don't contaminate your sections but only a few cowboys use their fingers in the water bath
He is handling fixed, dehydrated, xylene-treated, embedded blocks. He is not handling blood or fresh tissues in the video. Stop the safety paranoia.
Thank you!
Just FYI peeps. Certain tissue types need more hydration ( hence the variance in soaking times). If a tissue is dry for sectioning such as brain or liver, it needs to sit on wet ice for longer. If tissue is fatty such as deep melanomas, breast, lipomas etc soaking the block on water is about the biggest mistake you can make. It will interfere with sectioning if you introduce water after processing. It will be incompatible with xylene that is still present in the tissue and explode on the water bath. The whole point of soaking your block prior to sectioning is to hydrate the tissue so that the morphology is more accurate on the slide.
I have been taught to keep blocks on ice after trimming. Sections just fall off at 4microns
good thank you
nice
very good
Very Good ...
What type of tissue are u sectioning that you soak for 24 hrs? Is this for special studies or routine staining?