Пікірлер

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  @Ninja_Samurai-dd3kq4 күн бұрын

  Despite the video being useful, many steps are skipped, and the calculations are confusing.

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  @samcreativity62672 ай бұрын

  Incomplete procedure

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  @salmaallah37032 ай бұрын

  What is the wave length used to measure the OD using universal photometer?

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  @AakashKumar-yz5vz5 ай бұрын

  Which media is used for fastidious bacteria.

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  @nikhilbhalla68027 ай бұрын

  I am afraid my plate will dry out, will be keeping for at least 7 days

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  @nikhilbhalla68027 ай бұрын

  Labelling the plate like that will contaminate especially with slow growing bacteria. I guess I should skip it. I will be doing it with Mycobacterium tuberculosis

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  @zahidkhan55327 ай бұрын

  excellent explanation.....thanks

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  @kukamaiki180510 ай бұрын

  Please help me with a written outline of the procedure

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  @ifeoluwaidowu7692 Жыл бұрын

  How many ml of agar was prepared for each plate and what is the volume of the drug diluted in it?

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  @yemisioyetunde64 Жыл бұрын

  Thank you so much for this. I think the calculation was wrong at the point of 800microliters of the 1mg/mL antibiotic solution. it should be 80microlitres in 19.92 mL to obtain 4mcg/mL. The calculation would however be correct if it were a 0.1mg/mL antibiotic solution. But I give kudos to this simplified and practical explanation. Great job.

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  @yemisioyetunde64 Жыл бұрын

  On a second look, I think the concentration has been brought to what was actually labelled by diluting with 90 mL of agar. We can then say the 4mcg/mL in tube was actually 40mcg/mL and 10 mL of that was diluted with 90 mL of agar, to give 4mcg/mL. hence the agar dilution as its name implies

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  @sashanealand8315 Жыл бұрын

  what is the final volume in each well? 100ul or 200ul?

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  @yuwan Жыл бұрын

  The formula for calculating the weight of antibiotic powder given a potency seems wrong to me. Should it be "weight = V × C / P" to get the weight in mg when C is measured by mg/L or mg/1000mL?

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  @anjanakelum640120 күн бұрын

  Can you help me on this, how to calculate this value

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  @treasuresofartbytanushree999 Жыл бұрын

  Great great super great explanation...a person who doesn't know how to use a machine in healthcare...be it Fresher also ...it will make them feel they have enough knowledge to know stand in front of a machine and operate it at minimum stage atleast... thanku so much

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  @nailaimtiaz3617 Жыл бұрын

  Incubation done for how long?

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  @nailaimtiaz3617 Жыл бұрын

  Please make more video like MALDITOF

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  @nailaimtiaz3617 Жыл бұрын

  Where are you from pk I means this lab?

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  @Vijayhemmadiprakash Жыл бұрын

  I suscept there is something wrong with your calculations

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  @orumwensekensington99072 жыл бұрын

  Thanks for the lecture. Please, can I get some antibiotic disc fron you to enhance my research work.

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  @oussamamoussaoui75722 жыл бұрын

  I dont understand a word she is saying

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  @PrincessZoee7 ай бұрын

  She is speaking in english. You can translate

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  @babusheikh66522 жыл бұрын

  Hi there, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?

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  @kashifawan282 жыл бұрын

  Hello..can you tell how to interpret results of MIC?

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  @Ray4rafia Жыл бұрын

  Have you prepared results now? Can you please help me

• 
  @aribashamimakhtar96252 жыл бұрын

  from where i can get this kit in pakistan

• 
  @biotechnologyclassroom85302 жыл бұрын

  kzread.info/dron/H0t_rwtB0f9KqIga1HnQDg.html

• 
  @lakshmipriyaridevi10332 жыл бұрын

  Well 1A volume addition and dilution explanation is confusing.

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  @spider49572 жыл бұрын

  Composition?

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  @noorakbar76383 жыл бұрын

  How you adjusted the culture OD and how many bacteria were there per well? Kindly reply

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  @paz83903 жыл бұрын

  There are a densichek You take some culture and put it in a tube with saline medium. Then you place it in the densichek so that by absorbance it measures the OD at a 0.50

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  @mokhles7033 жыл бұрын

  i just wish you guys could speak proper english, thanks anyway.

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  @ayat45223 жыл бұрын

  can we increase number of discs in large or king size agar plate ?

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  @ayat45223 жыл бұрын

  thank a lot ✨

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  @sugamgautam17463 жыл бұрын

  kzread.info/dash/bejne/nWihzdp_dNO4lrQ.html

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  @gustavoalves49643 жыл бұрын

  Please, activate the caption setting. Congratulations for the video!

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  @shaskign3 жыл бұрын

  then there r 300 microliter antibiotic + 100 microliter CAMHB in well 1A? doesn't it supposed to be only 100 antibiotic + 100 CAMHB? It's confusing

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  @shafac62153 жыл бұрын

  Thank u so much, it was very helpful

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  @reemmaaroof21833 жыл бұрын

  شغل عالي و الله

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  @neetushekhawat9823 жыл бұрын

  Do u happen to know , how to like use phyto oils in mic . They probably wouldnt diffuse in the agar surface

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  @CoalCrafteris3 жыл бұрын

  spasibo mam

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  @gamesvova6753 жыл бұрын

  Beautiful

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  @shah74603 жыл бұрын

  Thanks

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  @zainabfatima79523 жыл бұрын

  Best vid maam 😊

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  @alb40793 жыл бұрын

  What if out of 3 wells, 1 well is turbid and 2 other wells are clean. What should you report?

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  @sruthics16043 жыл бұрын

  Thanku mam

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  @husseinkaka79643 жыл бұрын

  Can use other solvent than waterproof

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  @hussainzuhajamz14203 жыл бұрын

  Thnkn u mam❤️

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  @syedkashifraza80994 жыл бұрын

  Zabardast sir

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  @parnpakistan99074 жыл бұрын

  Thank you Syed Kahif Raza, keep visiting our website for more information and updates

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  @hclaboratoryinformation30774 жыл бұрын

  Why is blood added to the Mueller Hinton agar?

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  @parnpakistan99074 жыл бұрын

  blood added to support growth of fastidious organisms like streptococcus pneumoniae

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  @sulumolthomas26954 жыл бұрын

  i didnt understand the agar dilution method.if anyone please explain details

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  @umamakhan59124 жыл бұрын

  i didnt understand that in what should be 19.2 ml of DW added ? in 10 ml tubes or what???

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  @ManarNihonKankou4 жыл бұрын

  I think it's not 19.2 ml! It should be 19.2 µl

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  @parnpakistan99074 жыл бұрын

  First of the tube or container is labelled as 4 mcg/ml and others with 10 ml of distilled water are marked as 2, 1, 0.5 mcg and so on. To the first tube you add 19.2 ml of sterile distilled water and 800 ul of antibiotic stock this will give you 20 ml of first dilution ( 4mcg/ml) now serially transfer 10 ml to prepare the next dilutions

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  @bushraalayed49324 жыл бұрын

  @@parnpakistan9907 but how you got the 800 ul? what was the concentration of the antibiotic stock you used to make 4 mcg/ml dilution? please help

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  @ah75103 жыл бұрын

  @@parnpakistan9907 i think it is 80 ul to 19.92 ml DW

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  @Kanika489304 жыл бұрын

  Well explained video. Thank you. Ma'am from where did u get the protocol? Can i mail you if I have some questions?

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  @arshiamaryam78825 жыл бұрын

  Mam I want to take guideline from you kindly send me you email adress

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  @estefaniamarino28635 жыл бұрын

  Send me please. [email protected]

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  @mofigueroa5 жыл бұрын

  @@estefaniamarino2863 Did you receive the mail with de guideline??

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  @sam_khan_19955 жыл бұрын

  Hey sir need mailing addres. Love the facility it's wow! Would love to work there !