I would seeking for some easy understandable lessons about yeast growing and I found your lessons. thanks!

Some on commented, then deleted "shouldn't aseptic techniques include gloves?". Not sure why they deleted the question (its a good one), so I'll reply to it anyways. In the micro lab we always wear gloves if working with pathogens, and never wear gloves when working with non-pathogens. The reason is a balance of risk and benifits. Gloves provide minimal protection for your samples, as only dander/etc from your hands is blocked - stuff in the air, falling from your face/hair/etc are not impacted. So there is a negligible advantage in terms of protecting your sample. Gloves do provide a lot of advantage in protecting you, as hand contact is the most common source of self-contamination when working with microorganisms. But there are costs to wearing gloves - you lose dexterity, and if you pass the gloves too close to the flame they can melt to your hand or even catch fire, causing a serious burn. So you have to balance the risk of wearing gloves to the advantages they offer. For a brewer, the advantages are far outweighed by the potential risks; in other microbiology circumstances the risk:benifit ratio is different.

Amazing lessons all over your channel! Thanks!

0:03 Introduction, Aseptic Techniques. 0:18 Most important thing, clean and uncluttered workstation 0:38 Clean yourself up, wash hands, tips and tricks. 0:58 PSA on hydrating soap, vs antibacterial soaps. 1:14 Start working on your work area, and additional clean room techs. 1:35 Gloves, why, when and how to use them. 2:16 Working properly with your burner/lamp. Slow movements, spacing, etc. 3:33 Most important tools, the loop (and others) 4:42 Cooling loop to agar, or dish/cup of 70% alcohol. 5:47 Flame and working with tubes, keeping tube clean and sterile. 6:38 Flaming the tube, cap, whatever through flame. 7:04 Working with plates 7:42 Flipping gently a plate/dish 8:14 Working with plate with loop 9:10 Thanks for watching.

excellent video. thanks for sharing.

@SuiGenerisBrewing

10 жыл бұрын

Thanx!

Wow, this very useful and great information! At least for my needs... Thank you so very much for providing this knowledge, it will certainly be of use and help.

@SuiGenerisBrewing

6 жыл бұрын

Glad you found it useful!

Thanks for sharing for that great video.

@SuiGenerisBrewing

7 жыл бұрын

Glad you liked it

Very informative, also don’t some gloves have a residual powder on them? Seems like that would also cause problems

@SuiGenerisBrewing

3 жыл бұрын

Powders on the inside.

great video - can you make additional agar slants and store them until you have the yeast to culture them?

@SuiGenerisBrewing

3 жыл бұрын

They only last about 3 months in the fridge, so not really, unless you're expecting to do a lot with then short-term.

Will using 2 or more alcohol lamps aid the updrift and make the workspace bigger?

@SuiGenerisBrewing

9 жыл бұрын

I doubt it. Using a lamp limits your workspace - the area behind is essentially unusable as it is difficult to access materials there safely (i.e. without burning yourself). While more lamps would give a larger updraft, it would also make for a larger danger zone. Unless you are working with particularly large plates or flasks, a single lamp should be more than enough.

How would you inoculate flour for bread making?

@SuiGenerisBrewing

2 жыл бұрын

Suspend it (either dough, or dried yeast) in some sterile water, then spread it on the plate.

Is there any scenario where you would not advocate for quenching in alcohol and flaming off the loop? (Other than it's a little more work)

@SuiGenerisBrewing

7 жыл бұрын

No reason not to do it; just e careful you don't light the alcohol on fire.

What was the agar you were using??

@SuiGenerisBrewing

8 жыл бұрын

Wort-Agar. Make some wort using DME, with an O.G. of 1.004 to 1.008. Add 2% agar (by weight; i.e. 2g/100 ml; 5g/cup), sterilize and pour.