Translation Initiation in Eukaryotes
Eukaryotic translation is the biological process by which messenger RNA is translated into proteins in eukaryotes. It consists of four phases: initiation, elongation, termination, and recycling.
CAP-DEPENDENT AND CAP INDEPENDENT INITIATION PATHWAYS :
Initiation of translation usually involves the interaction of certain key proteins, the initiation factors, with a special tag bound to the 5'-end of an mRNA molecule, the 5' cap, as well as with the 5' UTR. These proteins bind the small (40S) ribosomal subunit and hold the mRNA in place. eIF3 is associated with the 40S ribosomal subunit and plays a role in keeping the large (60S) ribosomal subunit from prematurely binding. eIF3 also interacts with the eIF4F complex, which consists of three other initiation factors: eIF4A, eIF4E, and eIF4G. eIF4G is a scaffolding protein that directly associates with both eIF3 and the other two components.
The best-studied example of cap-independent translation initiation in eukaryotes uses the internal ribosome entry site (IRES). Unlike cap-dependent translation, cap-independent translation does not require a 5' cap to initiate scanning from the 5' end of the mRNA until the start codon.
Пікірлер: 127
It's 3am right now, I am pulling an all-nighter and feel so relieved to have FINALLY understood all the eIAFs in this mechanism. Thank you so much.
@hussainbiology
5 ай бұрын
most welcome...
i have my molecular biology exam in three days thanks for explaining this better than my professor
@hussainbiology
3 жыл бұрын
thanks Liza for appreciation... Really Glad to know that it helps...... All the best ahead
@malthekjrbendtsen194
3 жыл бұрын
Me too, good luck! :D
@hussainbiology
3 жыл бұрын
@@malthekjrbendtsen194 ❤️ All the best
very nice video in 6 minutes you have taught us so much that we were not able to understand in 1 hour class, what an amazing teacher
On 5th of January 2023 I'm having my final exam for molecular Biology (I'm doing Bsc in Biotechnology and Genetic Engineering currently 5th semester final exam gonna start). And this guy is really really something else.. I haven't understood from my teacher/professor but these lectures are so so so so well explained and easy to understand... A 100% recommended thing... Keep it up Pray for Me, 🤲
@hussainbiology
Жыл бұрын
thanks for appreciation.....and U will rock in exam... i am sure.... All the best
@huzaifa2k1
Жыл бұрын
@@hussainbiology Thank you for the motivation.... Jazakallah ❤️💜
@sreejabhattacharya4511
8 ай бұрын
@@huzaifa2k1Even I am doing B. Tech in Biotechnology and I don't understand a thing my professor teaches 😭.
So very helpful!!! Thank you so much 😆
YOU ARE DOING GREAT SIR
U are the best teacher for molecular biology.... Very easily u clear such tough concepts... Thankyou so much for all the topics u teach but please upload the vedio for elongation and termination process.. Your method make these topics very interesting so please sir complete this eukaryotic translation Thank you so much
@hussainbiology
2 жыл бұрын
those videos are already available .. plz check the playlist
Thank you for explaining such a complicated process in detail!
@hussainbiology
3 жыл бұрын
thanks for appreciation.. Glad to know that it helps ✌️
This is a great description. Thanks
@hussainbiology
2 жыл бұрын
thanks for appreciation
Ofcourse as usual awesome explanation 😀 thank you so much And waiting for further vedios 🤗
@hussainbiology
4 жыл бұрын
thanks Anamika for appreciation...Glad to know that it helps ✌️
Best molecular teacher ever 😍😍❤️ Thanku for sharing the video's,🙏🏻😍❤️
@hussainbiology
Жыл бұрын
thanks for appreciation.. Glad it helps ✌️
thanks alot ....may god bless you
@hussainbiology
3 ай бұрын
God bless u too
Thank you very much sir In a few minutes u have explained it so well
@hussainbiology
10 ай бұрын
You are most welcome
Amazing explanation...you are great 👍 👌
@hussainbiology
8 ай бұрын
Thanks a lot 😊
Sir plzzz upload next videos as early as possible plzzzzzzzz don't take long time , Plzzzzzzz upload two lectures in one week.
Thanks for this easy explanation 😊
@hussainbiology
3 ай бұрын
My pleasure 😊
Jazak Allah khair 🤲
@hussainbiology
3 жыл бұрын
✌️
amazing
Ur teaching is the best
@hussainbiology
2 жыл бұрын
thanks Aditi for appreciation..Glad it helps
amazing thank you
@hussainbiology
3 жыл бұрын
thanks Maryam for appreciation..Glad it helps ✌️
Thanx..it's really helpful for all students...👌
@hussainbiology
4 жыл бұрын
thanks for appreciation..Glad to know that it helps ✌️
Well explain better than other
@hussainbiology
Жыл бұрын
thanks for appreciation..Glad it helps....✌️
gold!
This video was good although I have some doubts 1. Where is eIF2B, eIF5 used??? (As it's binding and animation is not mentioned in this video) 2. can you please be more elaborate with eIF5 and eIF5B functioning. As in the previous video you've mentioned eIF5B dissociates all the factors from 80SrRNA (which acc. to me probably must occur at the ending step of initiation)but here in this video eIF5B attaches so early in the 40SrRNA.
Mashaallah sir Ultimate 👍
@hussainbiology
4 жыл бұрын
Thanks
How easily explain...nice class
@hussainbiology
3 жыл бұрын
thanks for appreciation
What is circulization of mRNA why we use it ,and what is their function and plzzzzz also told kozak sequence also
Bester Mann
Best sir
This video helped me alot❤️
@hussainbiology
2 жыл бұрын
thanks for appreciation..Glad it helps
Thanks sir for giving help 🙏🙏
@hussainbiology
2 жыл бұрын
It's my pleasure
😊😊😊😊😊thanku
Thankyou so much sir 😊🙏🇮🇳
@hussainbiology
4 жыл бұрын
you are welcome
Sir your videos are very helpful.It gives clear understanding of the topic Can you please make a video on elongation and termination of translation ?
@hussainbiology
Жыл бұрын
first of all thanks for appreciation... aur shyd maine Woh Videos banaye hai...
Sir Please Make video for CSIR-NET series. It will rise your subscriber count
model of transcription ,transient mode,l Inchworming model, and Scrunching model, function of ctd tail why it required.
Could u make the subtitle available please? Thanks
AOa sir , sir you read transcription if you read transcription where is videos of transcription elongation and termination in eukaryote plzzzzzzzzzzzz told and upload that videos also my exams are very near it,s humble request to upload transcription videos and next video early on you channel. Be happly doing great work .
plzzz upload next video
Big fan sir
@hussainbiology
2 жыл бұрын
So glad to know that ... Really appreciate your support ❤️
Very nice sir
@hussainbiology
7 ай бұрын
Keep watching
Sir nice video
@hussainbiology
2 жыл бұрын
✌️
Do u know prof. ashish sharma sir (ex faculty of GB), your clearity of concepts make me feel like you are his student.
@hussainbiology
3 жыл бұрын
Thanks for appreciation... But i ain't a student of Ashish Sir... i wish i too had great teacher's like u said of Ashish Sir....✌️✌️
plzzzzzzzzzz upload next video.
NIce
@hussainbiology
4 жыл бұрын
Thanks
👍
Sir can you please make a video on mitochondrial translation process
@hussainbiology
3 жыл бұрын
will consider Pooja
Thank u sir waitting next elongation and termination part
@hussainbiology
4 жыл бұрын
kzread.info/dash/bejne/m2SEq9WmqKuWZ7g.html
@hussainbiology
4 жыл бұрын
This animation will lead to Elongation and Termination...
Upload next video plzzz sir plzzzzzzz si
Where's the next part of this Video
at 3:16 isnt it supposed to be elf5 not elf5b?
Good day, sir you didn't mention about the property of eIF4B in this process.
@hussainbiology
3 жыл бұрын
. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
How does cap independent translation occur plzzzzzz explain it also.
@hussainbiology
4 жыл бұрын
Will be explaining soon. We had Internet Blockade from past week
sir you did not upload translation elongation and termination in eukaryote . plzzzzzzzzzzzzzzzzzzzzz explain post transcription modification along with group 1,2 ,3 4, . i knew that i requested many videos and you fulfilled thanx for uploading that realy it help me alot i have some problem in between that visero thatwhy i request you many time sorry if you irritate with my repeatedly request . your videos are ossum .
@hussainbiology
4 жыл бұрын
InshaAllah will be working on Post Translational Modifications from Next week.
@hussainbiology
4 жыл бұрын
There is no word of irritation but u need to understand as i am getting hundreds of requests to make videos on different niches , so i have to put forward everyone's request...Thanks and regards
@hussainbiology
4 жыл бұрын
Kindly Check the Animation of Translation where elongation and termination have been discussed in detail.
plz told whole termination I read watson but I can't understand their words
@hussainbiology
4 жыл бұрын
okay.
@ammarahchaudhary2820
4 жыл бұрын
Sir plzzzzzzzzz give your email I'd and that my e mail I'd is mehchh@gmail.com for answers of following questions that asked few hrs before.
Sir where is elongation and termination??? Plz rply
Subtitles should be given in english
@hussainbiology
Ай бұрын
okau
Thank you sooo much... But elongation and termination kte??
@hussainbiology
2 жыл бұрын
Playlist wuch... Tath menz sere video😊
@hussainbiology
2 жыл бұрын
email send krna apna ya kuch aur like insta or.. Jis pe mai woh aap ka doubt tha na.. i will make sure to send u those images regarding replication
@hussainbiology
2 жыл бұрын
kzread.info/dash/bejne/m2SEq9WmqKuWZ7g.html
@misbashabir6514
2 жыл бұрын
@@hussainbiology where I vll send yhii pe??
Sir, when we get elongation and termination video of eukaryotes.
@hussainbiology
3 жыл бұрын
already available...Plz check the playlist
@priyanshusharma2695
3 жыл бұрын
Ok sir
@priyanshusharma2695
3 жыл бұрын
Sir can you please mention that links here of elongation and termination of eukaryotic translation...
Can u please add the vedios for translation elongation and termination
@hussainbiology
3 жыл бұрын
kzread.info/dash/bejne/hp-Mp85ypdayfpc.html
@hussainbiology
3 жыл бұрын
It is short video..but will work
@darshanadilhan5717
3 жыл бұрын
I am asking about Eukariyotic translation elongation not about prokaryotic translation elongation Do they have the same mechanism?
@hussainbiology
3 жыл бұрын
@@darshanadilhan5717 kzread.info/dash/bejne/m2SEq9WmqKuWZ7g.html
@hussainbiology
3 жыл бұрын
This will help a bit till i make a complete detailed video
Sir Aa eIF4A , eIF4E & eIF4G MATHI E, G BIND THAI TO 4A KAI
@janvipatel1325
3 жыл бұрын
Jay 6e
I’m studying 📑 for hussan
@Jaybug20
7 ай бұрын
Okay I just wanted watch ❤
@hussainbiology
7 ай бұрын
thanks brother...Keep sharing
dude, please work on your accent
@hussainbiology
3 жыл бұрын
okay sir
@andreipotapenko8548
3 жыл бұрын
@@hussainbiology thanks, brother. Once again, the content is great. But I can't understand anything. Or, maybe, put some subtitles?
@hussainbiology
3 жыл бұрын
Yeah Surely.... I will work on accent as well as put subtitles