Splicing
RNA Splicing | group 1 introns | group 2 introns | self splicing | spliceosome |
The transcription of eukaryotic gene results in formation of pre-mRNA. The pre mRNA have coding sequences and non coding sequences. The coding sequences are known as the exons, while the non coding sequence are known as the introns. splicing is a phenomonon in which the introns are removed and the exons are joined togeather to form mature mRNA. the basic mechanism of splicing is simple. the phosphodiester bond between exon and intron at 5' splice site is first broken, then the 3' hydroxyl group of the 5' exon, reacts with the 3' splice site to complete the reaction.
Пікірлер: 154
it helped me in so many ways to better understanding how exactly the splicing takes place! thanks for the great job.
this is so far the most simplified yet the best video on the topic!!
Loved it! I never understood splicing until now. Keep it up my friend, you have an excellent way of explaining.
Thank you sir for such a simple yet completely clear and understandable lecture
To add a minor revision, you could substitute "dash" with "prime" word. Thank you !
@lorrainelolah6841
2 жыл бұрын
hello Okan! can i kindly have any questions on pre- mRNA that you might be studying. i actually approached you since your comment is the most recent.
@BioTechNomad
2 жыл бұрын
😂😂😂😂 i was thinking the same
@varnamohan2629
26 күн бұрын
We use prime
Great video! Super helpful, one thing to note is I believe the correct naming of the G and A nucleosides is "guanosine" and "adenosine" because they are joined with ribose, not on their own.
Best video on splicing
thank you, you made such a complicated process easy and clear to understand
Excellent explanation. Thank you so much!
Thank you so much for this video! I'm a visual and an auditory learner and I thought your explanation was perfect!
I'm so happy I found you. You are great!
It was quiet effortless to learn splicing from your video. Thanks a ton.
Really thank you for the video sir, I have never understood Splicing this good before
great video, explained very clearly thank you
Great explanation, thank you!
Great work 👍 very clearly explained
Thanks man.. I really get understand by your video a day before my exam.i cann't speak out how am i feeling now!!!!!!
Thanks man, I really appreciate this. Have a great day!
simplest and shortest explanation of all, very helpful
It's very helpful, thank you for this video
Now I clearly understand this concept thanks
Great video buddy. Thanks!
you explained something in 7 minutes that my professor couldn't in and hour and a half !!!!
The best explanation ever!
very short and to the point..thank u
Thank you.. its very helpful and easy to understand.
You are a god send. thank you.
Thank you for your help 🙏🙏
Thank you so the video.. It was very educating
Bless you,man!saved my day'
Thank you so much!
Very helpful. The simple explanation of attack here and there from my professor was not cutting it.
Very helpful!!! Thank you!!
Thank you so much
Great explanation..and . good vedio too.......
thanks for your help! you're pretty awesome!
thanks making this video mate !
I know I will never ever e-ever give you uuup, and I wanna say thank you in case I don't thank you enouuugh...
thaaabk you so much i spent three days trying to understand this, fortunately i found this and i understood it quicklyy so now i wish if i have founded this 3 days ago hahaha
Thank you for the video. Please can you help with an explanation why in-vitro splicing reconstituted with U-snRNPs is slower than in-vivo splicing? Thank you.
Helpful video. Thank you
Nicely explained 👍
Thanku so much for complete explanation
I did'nt understand my teacher's wording But this vedio help me and make me to understand splicing easily I just love thiss❤
Thank you
Waaaaaaao 👍
Thank you very easy to understand
Super explanation 👍
Very simple, Thanks
Really helpful thank you so much
Thank you sir , the video was easy to understand .
Tysm,, u really helped me a lot
Thank you🙏
Really helpful..... thank U so much....keep it up......!!❤🤗
Much helpful💜💜💜
thank you so much
thank you so much❣
Also Prokaryotes can't have introns, because they have transcription coupled to translation. They don't have time/space for that, since intron splicing will stop the coupling. Eukaryotes evolved the nucleus, where splicing can be done
@ganmeii8912
2 жыл бұрын
they still can have introns, prokaryotes perform self splicing which means it won’t impact translation time wise but it can still happen
GOATED.
Very well explained sir
Really very nice explaination
Loved it ....
Great video
Thankk you, it is very informative!
Thanks
ty
Why are there no videos of examples using a template strand to help us identify introns and exons
You are great bro 👍
Very nyz clz...easy to study😍❤️
Nice information of RNA splicing
Thanks sir
1:50 ✍️ It helped me a lot, thx🙏
I’ve never heard prime read as dash lol
@gretabecker2946
3 жыл бұрын
Me neither. Came to the comment section just to see if that's a thing... So, was this by accident or is it, indeed, called 5/3 dash in some other parts of the world?
@shubhangimorale9684
3 жыл бұрын
@@gretabecker2946 some people call it dash instead of prime which is conceptually wrong. It should always be 5’prime splice site.
@nadiatumayine7341
3 жыл бұрын
Thank you for letting me know that the dash meant prime, I was on my way to closing the video because I was even more confused :)
@londonjeremiah8434
2 жыл бұрын
instablaster...
@bookishmahsa6634
2 жыл бұрын
cool then I'm not alone
Good explanation thank you sir
Thanku so much ........
thanks for the help, great video.
AMAZING
U5 is attached to both the exons, holding them together
Thank❤❤❤❤❤❤
I love you sir thanks
Great sir
wow nice
am I the only one who thinks snurp is the cutest name ever?
loveeeeee you a lottttt god bless youuuuuu
Nice...
Great video! What is your primary source for this video? Thanks.
Tq so much sir i really appreciate you ❤️
So where does the guanine nucleotide in type 1 introns come from?
Thank you mam
you are gooooooood
Oh great
💓
excuse me, Mr. what's book you were used in this video ?
Great, just one comment, it's not 5 or 3 "slash" but "prime" (like Amazon, ok bad joke)
@saimsaim8580
4 жыл бұрын
😒😂😂😂
@kavyashree8069
3 жыл бұрын
He said 3 dash and 5 dash and not "slash". In most of the colleges and universities they teach us like that
@anshikajain7556
3 жыл бұрын
It's pronounced prime not slash in biology
@c0rtikoZteroids1
3 жыл бұрын
@@anshikajain7556 It's common nomenclature; "dash" and "prime" are both accepted.
@anshikajain7556
3 жыл бұрын
@@c0rtikoZteroids1 ik but i was talking about slash. Thanks btw
can somebody explain what is branching sequence in mRNA and why it is called as branching sequence
То чувство, когда говоришь по-русски а слушаешь по-английски и все понимаешь)
What happened to the introns after slicing?
so lariat forms only on group 2?
Is "five dash" the correct pronunciation of 5' ? 10yrs ago when I had taken courses in biochemistry and ever since then I've only heard it as "five prime". your video is the first time I've ever heard of "five dash".
Why the splicing of intron in transcription occur in eukaryotic not in prokaryotes?