Splicing

RNA Splicing | group 1 introns | group 2 introns | self splicing | spliceosome |
The transcription of eukaryotic gene results in formation of pre-mRNA. The pre mRNA have coding sequences and non coding sequences. The coding sequences are known as the exons, while the non coding sequence are known as the introns. splicing is a phenomonon in which the introns are removed and the exons are joined togeather to form mature mRNA. the basic mechanism of splicing is simple. the phosphodiester bond between exon and intron at 5' splice site is first broken, then the 3' hydroxyl group of the 5' exon, reacts with the 3' splice site to complete the reaction.

Пікірлер: 154

  • @sarvsoltani1674
    @sarvsoltani16744 жыл бұрын

    it helped me in so many ways to better understanding how exactly the splicing takes place! thanks for the great job.

  • @ummeabiha7323
    @ummeabiha73232 жыл бұрын

    this is so far the most simplified yet the best video on the topic!!

  • @CancerEnthusiast
    @CancerEnthusiast4 жыл бұрын

    Loved it! I never understood splicing until now. Keep it up my friend, you have an excellent way of explaining.

  • @ummulbanin9845
    @ummulbanin98453 жыл бұрын

    Thank you sir for such a simple yet completely clear and understandable lecture

  • @okankilic2297
    @okankilic22972 жыл бұрын

    To add a minor revision, you could substitute "dash" with "prime" word. Thank you !

  • @lorrainelolah6841

    @lorrainelolah6841

    2 жыл бұрын

    hello Okan! can i kindly have any questions on pre- mRNA that you might be studying. i actually approached you since your comment is the most recent.

  • @BioTechNomad

    @BioTechNomad

    2 жыл бұрын

    😂😂😂😂 i was thinking the same

  • @varnamohan2629

    @varnamohan2629

    26 күн бұрын

    We use prime

  • @rachl8400
    @rachl8400 Жыл бұрын

    Great video! Super helpful, one thing to note is I believe the correct naming of the G and A nucleosides is "guanosine" and "adenosine" because they are joined with ribose, not on their own.

  • @memanjoo005
    @memanjoo0053 ай бұрын

    Best video on splicing

  • @shamshassan6827
    @shamshassan68272 жыл бұрын

    thank you, you made such a complicated process easy and clear to understand

  • @yarelisrivas-avila7610
    @yarelisrivas-avila76103 жыл бұрын

    Excellent explanation. Thank you so much!

  • @SabrinaScience8
    @SabrinaScience83 жыл бұрын

    Thank you so much for this video! I'm a visual and an auditory learner and I thought your explanation was perfect!

  • @HadasLL
    @HadasLL2 жыл бұрын

    I'm so happy I found you. You are great!

  • @apekshadandi7767
    @apekshadandi77677 ай бұрын

    It was quiet effortless to learn splicing from your video. Thanks a ton.

  • @joyganguly6834
    @joyganguly683429 күн бұрын

    Really thank you for the video sir, I have never understood Splicing this good before

  • @paige9274
    @paige92743 жыл бұрын

    great video, explained very clearly thank you

  • @bracken7794
    @bracken77942 жыл бұрын

    Great explanation, thank you!

  • @Sunshine-Muskan
    @Sunshine-Muskan3 жыл бұрын

    Great work 👍 very clearly explained

  • @rajuislam2490
    @rajuislam2490 Жыл бұрын

    Thanks man.. I really get understand by your video a day before my exam.i cann't speak out how am i feeling now!!!!!!

  • @chloesmith2607
    @chloesmith26072 жыл бұрын

    Thanks man, I really appreciate this. Have a great day!

  • @KarthikDisley
    @KarthikDisley11 ай бұрын

    simplest and shortest explanation of all, very helpful

  • @apurbamukherjee794
    @apurbamukherjee7944 жыл бұрын

    It's very helpful, thank you for this video

  • @sharonbhambal2084
    @sharonbhambal20843 жыл бұрын

    Now I clearly understand this concept thanks

  • @eisenj21188
    @eisenj211883 жыл бұрын

    Great video buddy. Thanks!

  • @razanfeki2909
    @razanfeki2909 Жыл бұрын

    you explained something in 7 minutes that my professor couldn't in and hour and a half !!!!

  • @aaronmbwabi
    @aaronmbwabi Жыл бұрын

    The best explanation ever!

  • @md.ashrafulkarim6778
    @md.ashrafulkarim67784 жыл бұрын

    very short and to the point..thank u

  • @neethugopal2351
    @neethugopal23514 жыл бұрын

    Thank you.. its very helpful and easy to understand.

  • @user-me2uu4gy8w
    @user-me2uu4gy8w2 ай бұрын

    You are a god send. thank you.

  • @akashsahu6856
    @akashsahu68563 жыл бұрын

    Thank you for your help 🙏🙏

  • @linagabriel6356
    @linagabriel63564 жыл бұрын

    Thank you so the video.. It was very educating

  • @mmmpr2261
    @mmmpr22612 жыл бұрын

    Bless you,man!saved my day'

  • @liutianchanzwiers3706
    @liutianchanzwiers37069 күн бұрын

    Thank you so much!

  • @lordhelmchen6539
    @lordhelmchen65392 жыл бұрын

    Very helpful. The simple explanation of attack here and there from my professor was not cutting it.

  • @leyiwang3863
    @leyiwang38634 жыл бұрын

    Very helpful!!! Thank you!!

  • @mobarkalwahishi3323
    @mobarkalwahishi33234 жыл бұрын

    Thank you so much

  • @ganeshgr616
    @ganeshgr6163 жыл бұрын

    Great explanation..and . good vedio too.......

  • @lucyxiong7301
    @lucyxiong73013 жыл бұрын

    thanks for your help! you're pretty awesome!

  • @danielolumese9083
    @danielolumese90833 жыл бұрын

    thanks making this video mate !

  • @Reah850
    @Reah8503 жыл бұрын

    I know I will never ever e-ever give you uuup, and I wanna say thank you in case I don't thank you enouuugh...

  • @salmasanad710
    @salmasanad7104 жыл бұрын

    thaaabk you so much i spent three days trying to understand this, fortunately i found this and i understood it quicklyy so now i wish if i have founded this 3 days ago hahaha

  • @josephineobi8089
    @josephineobi80893 жыл бұрын

    Thank you for the video. Please can you help with an explanation why in-vitro splicing reconstituted with U-snRNPs is slower than in-vivo splicing? Thank you.

  • @joshmemarrynagdey1639
    @joshmemarrynagdey1639 Жыл бұрын

    Helpful video. Thank you

  • @drajiv1
    @drajiv13 жыл бұрын

    Nicely explained 👍

  • @the_explorer_anje8243
    @the_explorer_anje82434 жыл бұрын

    Thanku so much for complete explanation

  • @pakeezahashraf197
    @pakeezahashraf197 Жыл бұрын

    I did'nt understand my teacher's wording But this vedio help me and make me to understand splicing easily I just love thiss❤

  • @arshbir1539
    @arshbir1539 Жыл бұрын

    Thank you

  • @amnarehman1278
    @amnarehman12782 жыл бұрын

    Waaaaaaao 👍

  • @khoinguyen0210
    @khoinguyen02103 жыл бұрын

    Thank you very easy to understand

  • @sujankumar3620
    @sujankumar36203 жыл бұрын

    Super explanation 👍

  • @mawadaa.nasser651
    @mawadaa.nasser6512 жыл бұрын

    Very simple, Thanks

  • @prastutidebi8034
    @prastutidebi80342 жыл бұрын

    Really helpful thank you so much

  • @rajkishoredas936
    @rajkishoredas9362 жыл бұрын

    Thank you sir , the video was easy to understand .

  • @shaharbanupp6975
    @shaharbanupp69753 жыл бұрын

    Tysm,, u really helped me a lot

  • @bharatidas2108
    @bharatidas21082 жыл бұрын

    Thank you🙏

  • @yashusingh2289
    @yashusingh22893 жыл бұрын

    Really helpful..... thank U so much....keep it up......!!❤🤗

  • @ayshamalik2963
    @ayshamalik29634 жыл бұрын

    Much helpful💜💜💜

  • @Suche13
    @Suche134 жыл бұрын

    thank you so much

  • @muhammadtouseeftahir9477
    @muhammadtouseeftahir94772 жыл бұрын

    thank you so much❣

  • @alfredomunoz8791
    @alfredomunoz87914 жыл бұрын

    Also Prokaryotes can't have introns, because they have transcription coupled to translation. They don't have time/space for that, since intron splicing will stop the coupling. Eukaryotes evolved the nucleus, where splicing can be done

  • @ganmeii8912

    @ganmeii8912

    2 жыл бұрын

    they still can have introns, prokaryotes perform self splicing which means it won’t impact translation time wise but it can still happen

  • @mernas1418
    @mernas14182 ай бұрын

    GOATED.

  • @niks8924
    @niks89243 жыл бұрын

    Very well explained sir

  • @vaishallychugh8577
    @vaishallychugh85773 жыл бұрын

    Really very nice explaination

  • @mohanaburman8467
    @mohanaburman84673 жыл бұрын

    Loved it ....

  • @asmita_1999
    @asmita_1999 Жыл бұрын

    Great video

  • @Wilderness5283
    @Wilderness5283 Жыл бұрын

    Thankk you, it is very informative!

  • @Mostafa-hz4kj
    @Mostafa-hz4kj3 ай бұрын

    Thanks

  • @richardshane456
    @richardshane4564 жыл бұрын

    ty

  • @silva0003
    @silva00033 жыл бұрын

    Why are there no videos of examples using a template strand to help us identify introns and exons

  • @user-mh3mn9ge2b
    @user-mh3mn9ge2b11 ай бұрын

    You are great bro 👍

  • @anjanasaji6557
    @anjanasaji65572 жыл бұрын

    Very nyz clz...easy to study😍❤️

  • @funnyvideomarwadi
    @funnyvideomarwadi2 жыл бұрын

    Nice information of RNA splicing

  • @karansahare1349
    @karansahare13494 жыл бұрын

    Thanks sir

  • @user-tf2vh3jm1x
    @user-tf2vh3jm1x2 жыл бұрын

    1:50 ✍️ It helped me a lot, thx🙏

  • @extatickiddo5714
    @extatickiddo57143 жыл бұрын

    I’ve never heard prime read as dash lol

  • @gretabecker2946

    @gretabecker2946

    3 жыл бұрын

    Me neither. Came to the comment section just to see if that's a thing... So, was this by accident or is it, indeed, called 5/3 dash in some other parts of the world?

  • @shubhangimorale9684

    @shubhangimorale9684

    3 жыл бұрын

    @@gretabecker2946 some people call it dash instead of prime which is conceptually wrong. It should always be 5’prime splice site.

  • @nadiatumayine7341

    @nadiatumayine7341

    3 жыл бұрын

    Thank you for letting me know that the dash meant prime, I was on my way to closing the video because I was even more confused :)

  • @londonjeremiah8434

    @londonjeremiah8434

    2 жыл бұрын

    instablaster...

  • @bookishmahsa6634

    @bookishmahsa6634

    2 жыл бұрын

    cool then I'm not alone

  • @manjusamyuktha2013
    @manjusamyuktha20132 жыл бұрын

    Good explanation thank you sir

  • @manibio2948
    @manibio29483 жыл бұрын

    Thanku so much ........

  • @valentinapaolillo6310
    @valentinapaolillo63104 жыл бұрын

    thanks for the help, great video.

  • @user8582
    @user85822 жыл бұрын

    AMAZING

  • @ishanbharti8797
    @ishanbharti8797 Жыл бұрын

    U5 is attached to both the exons, holding them together

  • @HarshitKumarSinghAnine
    @HarshitKumarSinghAnine Жыл бұрын

    Thank❤❤❤❤❤❤

  • @durgamthirupathi1838
    @durgamthirupathi18384 жыл бұрын

    I love you sir thanks

  • @ahmadansari6829
    @ahmadansari6829 Жыл бұрын

    Great sir

  • @deebos980
    @deebos9803 жыл бұрын

    wow nice

  • @Alkimachos
    @Alkimachos Жыл бұрын

    am I the only one who thinks snurp is the cutest name ever?

  • @manikhalder3520
    @manikhalder3520 Жыл бұрын

    loveeeeee you a lottttt god bless youuuuuu

  • @sumanpanigrahi7275
    @sumanpanigrahi7275 Жыл бұрын

    Nice...

  • @mitchellhayes2400
    @mitchellhayes2400 Жыл бұрын

    Great video! What is your primary source for this video? Thanks.

  • @240_mandakinisabar4
    @240_mandakinisabar42 жыл бұрын

    Tq so much sir i really appreciate you ❤️

  • @zoom-zip3473
    @zoom-zip3473 Жыл бұрын

    So where does the guanine nucleotide in type 1 introns come from?

  • @subhasishbhattacharjee8596
    @subhasishbhattacharjee8596 Жыл бұрын

    Thank you mam

  • @manikhalder3520
    @manikhalder3520 Жыл бұрын

    you are gooooooood

  • @Prettiest_Aayat
    @Prettiest_Aayat Жыл бұрын

    Oh great

  • @ankitcreations9678
    @ankitcreations9678 Жыл бұрын

    💓

  • @syafianursyifa5183
    @syafianursyifa51833 жыл бұрын

    excuse me, Mr. what's book you were used in this video ?

  • @beatrizvelazquez5430
    @beatrizvelazquez54304 жыл бұрын

    Great, just one comment, it's not 5 or 3 "slash" but "prime" (like Amazon, ok bad joke)

  • @saimsaim8580

    @saimsaim8580

    4 жыл бұрын

    😒😂😂😂

  • @kavyashree8069

    @kavyashree8069

    3 жыл бұрын

    He said 3 dash and 5 dash and not "slash". In most of the colleges and universities they teach us like that

  • @anshikajain7556

    @anshikajain7556

    3 жыл бұрын

    It's pronounced prime not slash in biology

  • @c0rtikoZteroids1

    @c0rtikoZteroids1

    3 жыл бұрын

    @@anshikajain7556 It's common nomenclature; "dash" and "prime" are both accepted.

  • @anshikajain7556

    @anshikajain7556

    3 жыл бұрын

    @@c0rtikoZteroids1 ik but i was talking about slash. Thanks btw

  • @akashbaburaj2349
    @akashbaburaj23493 жыл бұрын

    can somebody explain what is branching sequence in mRNA and why it is called as branching sequence

  • @user-sy6jx7cw7m
    @user-sy6jx7cw7m Жыл бұрын

    То чувство, когда говоришь по-русски а слушаешь по-английски и все понимаешь)

  • @taniabhadra9313
    @taniabhadra93133 жыл бұрын

    What happened to the introns after slicing?

  • @mimineelam1671
    @mimineelam1671 Жыл бұрын

    so lariat forms only on group 2?

  • @mashedtoilet3033
    @mashedtoilet3033 Жыл бұрын

    Is "five dash" the correct pronunciation of 5' ? 10yrs ago when I had taken courses in biochemistry and ever since then I've only heard it as "five prime". your video is the first time I've ever heard of "five dash".

  • @muhammadabdullah4121
    @muhammadabdullah4121 Жыл бұрын

    Why the splicing of intron in transcription occur in eukaryotic not in prokaryotes?