siRNA mechanism | short interfering RNA mediated gene silencing
This lecture is about sirna mechanism and transfection process for sirna mediated gene silencing.
For more information, log on to-
www.shomusbiology.com/
Get Shomu's Biology DVD set here-
www.shomusbiology.com/dvd-store/
Download the study materials here-
shomusbiology.com/bio-material...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in KZread. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.com/1136485849827...
LinkedIn - / suman-bhattacharjee-2a...
KZread- / thefunsuman
Thank you for watching
Пікірлер: 167
PLEASE BECOME A PROFESSOR. I learn more from your channel then from my professors.
@shomusbiologyofficial
7 жыл бұрын
+Scott Amon thank you. Glad you liked my lectures
@nutribox4535
5 жыл бұрын
agree
@anamikamazumdar6147
3 жыл бұрын
Totally agree.
Suman, I have watched all the videos of rnai, you are a true genius Suman, God bless you always
@shomusbiologyofficial
5 жыл бұрын
Thank you so much for appreciating my efforts
I have been following you from last years of my clg life, I'm a Micro stud and wanted to tell you that a lot and lot of students of NBU are crazy about you - it's like if we want to know any topic in deep ,we simply log in to you ...just to let you know that a hell lot of clg studs watch you and we talk about you and we all have seen the journey of your channel.... Thank you so much for being with us from our first yr to last one ❤
All the 4 videos of RNAi are awesome, it cleared my doubts regarding RNAi, thank you Sir.
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you so much, this is such a wonderful and clear lecture! You really explained everything perfectly well. Thanks for your effort!
Thank you very very much for your detail introduction of RNAi machanism! you are the best teacher.
You are a competent teacher.. And know how to convey your information in easier ways.. Thank you
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures
dude you rock this was such an immense help!!!!
I'm a med student and you helped me a lot about understanding the siRNA. Many thanks.
@shomusbiologyofficial
Жыл бұрын
Thank you so much for appreciating my efforts to help
Sir,You made me fall in love with Life Science.
@shomusbiologyofficial
4 жыл бұрын
Thank you so much for appreciating my efforts
Thanks to you I am doing better in my QBM class at UCF.
Highly admirable teaching approach Suman, Keep it up. Very easy to understand such a complex process particularly for a beginner, applauds!!!!!!!
Dada u r just too awesome,pls keep helping us n god bless u
no wrds for ur works ......really impressive ☺☺
very helpful and very nicely explained.... thank you
Brilliantly explain the topic...👏👏👏
khub bhalo...sir ...this is the first time i am relying on any video lecture ...and its really fruitful.
@shomusbiologyofficial
7 жыл бұрын
+SHIPRA GOSWAMI glad I can help. Stay tuned
u r d best.. sir thank you so mch fr ds amazng descriptn abt Rnai
Sir, thank u very much you saved my time and made very easy to learn these things and watched your lectures more to study my molecular pharmacology subject.
@shomusbiologyofficial
5 жыл бұрын
Glad to hear that you are getting benefit from the videos
thank you very much. very good explanation.
Respect for you.
auspicious video dada....
I am learning all my topics from one and only yours channel seriiously. First thing first that comes in mind when i have a problem in any topic is "shomus biology". Thank you so much. 😍 love from Pakistan! Stay blessed 🤞
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Super very understanding thanks
very useful sir thank you so much
4 years ago, when I began to look for jobs, I studied on your channel. Now I am looking to change my job, I studied on your channel again. Thank you!
@shomusbiologyofficial
3 жыл бұрын
You're welcome. I am glad to hear that you're getting benefit from my lectures
thank you so much sir really helpful
Hey you are amazing in teaching and make concept so clear it help me a lot in my net preparation. Do you have ecology videos also If so please reply??
Very useful sir, thank you sir
Best video I have seen on this topic... thanks a lot
@shomusbiologyofficial
4 жыл бұрын
You're welcome
very nice teaching sir.....
gr8 how to do it practically on any cell..please show practical video or give us link
sir, RNase III is endonuclease so how can it cleave from ends (sides)??
Suman, thank you so much for your videos. They really help to understand the concepts thoroughly!
@irfanbashir4958
7 жыл бұрын
Ariba jo
Crystal clear. Great job.
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts
Your videos are really a great help. Wanted to ask: Can we Use RNAi in immunising the cell against the RNA/ genetic information of HIV viruses?
Great, practice a bit on process flow fluency avoiding corrections and hesitations you have mastery over the subject
In your RNAi video you mentioned Argonaut to be RNAse type 3 but here you said Argonaut is RNAse H type. So which one is correct?
THANK YOU SIR for teaching this difficult topic in such a simple way .
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts
Can anyone explain what is the difference between Dicer and Drosha (also Slicer/Pasha)?
Thank you dude
Thanku so much sir for your nice explanation 🙏
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures
very helpful 😊
Thank you so much!!!!! :)
Thank u so much sir , this topic is to easy to understand and thank u again
@shomusbiologyofficial
5 жыл бұрын
Glad to hear that you are getting benefit from the videos
U r best teacher.... thnkuuu so much fr making this concept so easy😊😊
@shomusbiologyofficial
5 жыл бұрын
Thank you for appreciating my efforts. Glad to hear that you are getting benefit from the videos
@vandnakapil889
5 жыл бұрын
Shomu's Biology .. this is ur hard work... u gave us best video lectures on every topic...
doesn't the helicase come into play in mi RNA processing?? pls specify as I'm having doubt
Thanku so much for such a valuable lacture.😀🤗
@shomusbiologyofficial
2 жыл бұрын
You're welcome
Thanks sir ........
really amazing very clear concept.
@shomusbiologyofficial
3 жыл бұрын
Thank you
Thank you sir.Your videos help me a lot😊
@shomusbiologyofficial
Жыл бұрын
You're welcome
wow.....Great explanation.....tq somuch...... .
@shomusbiologyofficial
3 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
you are just fabulous.
@shomusbiologyofficial
5 жыл бұрын
Thank you for watching the lecture
Amazing! Thank you ☺️☺️☺️!!
@shomusbiologyofficial
5 жыл бұрын
You're welcome
sir, its Very knowledge full video, it makes my whole concept clear about siRNA but i wanna know about the various types of siRNA like survivin and many more. So can u pls help me sir
@shomusbiologyofficial
4 жыл бұрын
Thank you. Glad you liked my lectures
Very nice
You made my day
@shomusbiologyofficial
4 жыл бұрын
Thank you
Bigger thanks for you Gad bless you 🙏🙏🙏🙏
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts
Sir...If Dicer has endonuclease activity then why is it chopping off the siRNA from the sides as 9:30? Please explain it Sir. Thank you.
Cloud you please do videos on MLS 4550 Clinical Immunohematology?
thank you for your trainer videos. I want to shoot video about siRNA designing on a specific example
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
should it actually be that coding and non-coding strand of DNA is being transcribed, so RNA can base pair not transcription of RNA?
very nice
Thank you very much, you are amazing. I have been following your tutorials for a long time. But in this video I think there is a mistake, you said RISC is constructed of slicer and Argonaut, this is a mistake because the slicer is Argonaut, but other parts are components like GW182.
Very nice presentation
@shomusbiologyofficial
3 жыл бұрын
Thank you
Which strand remained attached to the RISC complex if it would of been the antisense one then and only then we get an inhibition effect of target mRNA.. what if that strand is a sense strand.. then the whole process will be futile?.. plz answer the query.. it was a helpful video though..
Amazing explanation 👏🏻
@shomusbiologyofficial
5 жыл бұрын
Thank you
Sir whether the double stranded rna is produced from the target sequence and it will cleave the same target mrna
what about the guide rna strand?? after cleaving the target rna strand what happens to the guide rna
bro you say that in miRNA after forming RISC there are two domains one is paz that attach with single stranded mRNA & another is pivi domain have RNase activity..OK in all type of so,sh,mi RNA strands are unwinds by helicase?? are some factors involved??
Hi Suman sir...I feel the extreme joy of learning while watching your videos....Can you please make a video on RNA i antagonist process .....how the plant viruses suppress the RNA i ....
@shomusbiologyofficial
3 жыл бұрын
Thank you. I will do that in a separate video
@anuhyavalluri4881
3 жыл бұрын
Thank you sir....your efforts are much appreciated...
What are the intranuclear processing of a ds rna before leaving the nucleus??
No words to express 🤗
@shomusbiologyofficial
Жыл бұрын
Thank you
fabulous sir
@shomusbiologyofficial
7 жыл бұрын
+Arpan Chakrabarty you're welcome
sir most of the plant viruses are single standed so what is the use of rna silencing in case of plant-virus system
Sir does dsrna produce when the plant get infected with virus or its present already bin plant???
Sir, can u tell me if this can work in acellular organisms
Excellent sir tq sooo much 🙏🙏🙏🙏🙏🙏🙏🙏🙏
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
No doubt great teacher you are😊..but plz make it clear that..only siRNA need NPC complex for its transportation (from nucleus to cytoplasm) or miRNA and shRNA also need????
What will happen to the passenger strand? whether its get degraded or not?
my overseas professor of all time
@shomusbiologyofficial
5 жыл бұрын
Thank you
Once I had dumped this rna interference part coz it was so hard..but now I understood everything and its easy..thanku so much sir..u are really awesome.
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
very clear
@shomusbiologyofficial
3 жыл бұрын
Thank you
You are outstandingggggg. Love from Pakistan👍subscribed.
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
9.23 endonuclease...how??dicer is cutting from the end...then how?
RNase III is a endonuclease enzyme and how it able to cut in the sides or in the end of the strands ?????
Sir, which books are u referred ? Plz tell me sir....it will help for my research work
Many thanks for the video. Very informative as usual. However, if I'm not wrong, there is a mistake. In the RNAi introductory video, you say Dicer is RNase H, but you say Argonaut is RNase H in this video.
dicer(RNase III) cleave ds RNA in pre mi RNA in cytoplasm and drasha cleave mi RNA in pri mi RNA form..
have u cleared net jrf but very nicely and intelligently done
Amazing explanation about interference mechanism thanks sir
How double stranded Rna is introduced
nice!
@shomusbiologyofficial
Жыл бұрын
Thank you
If so rna block the expression of m-RNA then how normal translational takes place
How we send DS RNA into plant
very useful thnks
@shomusbiologyofficial
6 жыл бұрын
+sau Kamble you're welcome
@savitakamble4854
6 жыл бұрын
☺
RISC , (RNA induce silencing complex) not RNA interfering silencing complex
@shomusbiologyofficial
6 жыл бұрын
Yes. Thanks for the correction.
@Megha-ij8eo
3 жыл бұрын
Yes...u r correct...its RNA induced silencing complex...👍
This method use in Corona disease ??????
One requesting for make pi or Piwi rna
After cleaving the mRNA , whats the fate of guide strand further??