siRNA mechanism | short interfering RNA mediated gene silencing

This lecture is about sirna mechanism and transfection process for sirna mediated gene silencing.
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Thank you for watching

Пікірлер: 167

  • @scottamon8908
    @scottamon89087 жыл бұрын

    PLEASE BECOME A PROFESSOR. I learn more from your channel then from my professors.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +Scott Amon thank you. Glad you liked my lectures

  • @nutribox4535

    @nutribox4535

    5 жыл бұрын

    agree

  • @anamikamazumdar6147

    @anamikamazumdar6147

    3 жыл бұрын

    Totally agree.

  • @jovialdyalsingh4106
    @jovialdyalsingh41065 жыл бұрын

    Suman, I have watched all the videos of rnai, you are a true genius Suman, God bless you always

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you so much for appreciating my efforts

  • @shibrajpradhan6849
    @shibrajpradhan68493 жыл бұрын

    I have been following you from last years of my clg life, I'm a Micro stud and wanted to tell you that a lot and lot of students of NBU are crazy about you - it's like if we want to know any topic in deep ,we simply log in to you ...just to let you know that a hell lot of clg studs watch you and we talk about you and we all have seen the journey of your channel.... Thank you so much for being with us from our first yr to last one ❤

  • @thetravellingengineer3735
    @thetravellingengineer37354 жыл бұрын

    All the 4 videos of RNAi are awesome, it cleared my doubts regarding RNAi, thank you Sir.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @HaidaSOD
    @HaidaSOD9 жыл бұрын

    Thank you so much, this is such a wonderful and clear lecture! You really explained everything perfectly well. Thanks for your effort!

  • @yuchuntsao0609
    @yuchuntsao06099 жыл бұрын

    Thank you very very much for your detail introduction of RNAi machanism! you are the best teacher.

  • @zunairakiran7636
    @zunairakiran76364 жыл бұрын

    You are a competent teacher.. And know how to convey your information in easier ways.. Thank you

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Glad to hear that you're getting benefit from my lectures

  • @BioVossy
    @BioVossy8 жыл бұрын

    dude you rock this was such an immense help!!!!

  • @periophthalmus3740
    @periophthalmus3740 Жыл бұрын

    I'm a med student and you helped me a lot about understanding the siRNA. Many thanks.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Thank you so much for appreciating my efforts to help

  • @mayukhroy5393
    @mayukhroy53934 жыл бұрын

    Sir,You made me fall in love with Life Science.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you so much for appreciating my efforts

  • @esmeraldasish
    @esmeraldasish7 жыл бұрын

    Thanks to you I am doing better in my QBM class at UCF.

  • @narayanyadav3132
    @narayanyadav31328 жыл бұрын

    Highly admirable teaching approach Suman, Keep it up. Very easy to understand such a complex process particularly for a beginner, applauds!!!!!!!

  • @rumiirum1143
    @rumiirum11436 жыл бұрын

    Dada u r just too awesome,pls keep helping us n god bless u

  • @niazmughal3394
    @niazmughal33947 жыл бұрын

    no wrds for ur works ......really impressive ☺☺

  • @sneha-zb1yg
    @sneha-zb1yg9 жыл бұрын

    very helpful and very nicely explained.... thank you

  • @vijayasrivastava1928
    @vijayasrivastava19284 жыл бұрын

    Brilliantly explain the topic...👏👏👏

  • @MindandAyurveda
    @MindandAyurveda7 жыл бұрын

    khub bhalo...sir ...this is the first time i am relying on any video lecture ...and its really fruitful.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +SHIPRA GOSWAMI glad I can help. Stay tuned

  • @jilmilmahanta3669
    @jilmilmahanta36697 жыл бұрын

    u r d best.. sir thank you so mch fr ds amazng descriptn abt Rnai

  • @kirancu4713
    @kirancu47135 жыл бұрын

    Sir, thank u very much you saved my time and made very easy to learn these things and watched your lectures more to study my molecular pharmacology subject.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

  • @karanamraghavendra6848
    @karanamraghavendra68489 жыл бұрын

    thank you very much. very good explanation.

  • @rajlaxmiprasad7087
    @rajlaxmiprasad70873 жыл бұрын

    Respect for you.

  • @BalaBiologyWorld
    @BalaBiologyWorld7 жыл бұрын

    auspicious video dada....

  • @thio2620
    @thio26203 жыл бұрын

    I am learning all my topics from one and only yours channel seriiously. First thing first that comes in mind when i have a problem in any topic is "shomus biology". Thank you so much. 😍 love from Pakistan! Stay blessed 🤞

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

  • @firdausgulashan9228
    @firdausgulashan92286 жыл бұрын

    Super very understanding thanks

  • @divyagopal-natyam
    @divyagopal-natyam6 жыл бұрын

    very useful sir thank you so much

  • @pineapple4311
    @pineapple43113 жыл бұрын

    4 years ago, when I began to look for jobs, I studied on your channel. Now I am looking to change my job, I studied on your channel again. Thank you!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. I am glad to hear that you're getting benefit from my lectures

  • @nimomalik3866
    @nimomalik38665 жыл бұрын

    thank you so much sir really helpful

  • @reenukashyap1068
    @reenukashyap10689 жыл бұрын

    Hey you are amazing in teaching and make concept so clear it help me a lot in my net preparation. Do you have ecology videos also If so please reply??

  • @umashankarpatel4366
    @umashankarpatel43666 жыл бұрын

    Very useful sir, thank you sir

  • @tribenisharma1879
    @tribenisharma18794 жыл бұрын

    Best video I have seen on this topic... thanks a lot

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @gokulkrishnan1371
    @gokulkrishnan13718 жыл бұрын

    very nice teaching sir.....

  • @mikesinh
    @mikesinh8 жыл бұрын

    gr8 how to do it practically on any cell..please show practical video or give us link

  • @preeti4786
    @preeti47865 жыл бұрын

    sir, RNase III is endonuclease so how can it cleave from ends (sides)??

  • @AA-ne1fv
    @AA-ne1fv9 жыл бұрын

    Suman, thank you so much for your videos. They really help to understand the concepts thoroughly!

  • @irfanbashir4958

    @irfanbashir4958

    7 жыл бұрын

    Ariba jo

  • @rajeshkakkar8024
    @rajeshkakkar80243 жыл бұрын

    Crystal clear. Great job.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @harshoza1399
    @harshoza13997 жыл бұрын

    Your videos are really a great help. Wanted to ask: Can we Use RNAi in immunising the cell against the RNA/ genetic information of HIV viruses?

  • @markrao9216
    @markrao92166 жыл бұрын

    Great, practice a bit on process flow fluency avoiding corrections and hesitations you have mastery over the subject

  • @ramisharahman8813
    @ramisharahman88133 жыл бұрын

    In your RNAi video you mentioned Argonaut to be RNAse type 3 but here you said Argonaut is RNAse H type. So which one is correct?

  • @swatichauhan2531
    @swatichauhan25313 жыл бұрын

    THANK YOU SIR for teaching this difficult topic in such a simple way .

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @Mertule93
    @Mertule938 жыл бұрын

    Can anyone explain what is the difference between Dicer and Drosha (also Slicer/Pasha)?

  • @sijiphilip9618
    @sijiphilip96187 жыл бұрын

    Thank you dude

  • @sairam41053
    @sairam410534 жыл бұрын

    Thanku so much sir for your nice explanation 🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Glad to hear that you're getting benefit from my lectures

  • @SUMITKUMAR-ws7rs
    @SUMITKUMAR-ws7rs5 жыл бұрын

    very helpful 😊

  • @aizaibelalima1064
    @aizaibelalima10648 жыл бұрын

    Thank you so much!!!!! :)

  • @myworld4972
    @myworld49725 жыл бұрын

    Thank u so much sir , this topic is to easy to understand and thank u again

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

  • @vandnakapil889
    @vandnakapil8895 жыл бұрын

    U r best teacher.... thnkuuu so much fr making this concept so easy😊😊

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you for appreciating my efforts. Glad to hear that you are getting benefit from the videos

  • @vandnakapil889

    @vandnakapil889

    5 жыл бұрын

    Shomu's Biology .. this is ur hard work... u gave us best video lectures on every topic...

  • @abhiluitsharma1355
    @abhiluitsharma13556 жыл бұрын

    doesn't the helicase come into play in mi RNA processing?? pls specify as I'm having doubt

  • @dharampalkamboj6324
    @dharampalkamboj63242 жыл бұрын

    Thanku so much for such a valuable lacture.😀🤗

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

  • @PankajSinghNishad9691
    @PankajSinghNishad96915 жыл бұрын

    Thanks sir ........

  • @psrivastava6993
    @psrivastava69933 жыл бұрын

    really amazing very clear concept.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @lovelybarbhuiya9554
    @lovelybarbhuiya9554 Жыл бұрын

    Thank you sir.Your videos help me a lot😊

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    You're welcome

  • @ganeshgr616
    @ganeshgr6163 жыл бұрын

    wow.....Great explanation.....tq somuch...... .

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @bdhome2010
    @bdhome20105 жыл бұрын

    you are just fabulous.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you for watching the lecture

  • @jagmeetkaur2190
    @jagmeetkaur21905 жыл бұрын

    Amazing! Thank you ☺️☺️☺️!!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @anjalisingh6036
    @anjalisingh60364 жыл бұрын

    sir, its Very knowledge full video, it makes my whole concept clear about siRNA but i wanna know about the various types of siRNA like survivin and many more. So can u pls help me sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you. Glad you liked my lectures

  • @srijamukherjee1394
    @srijamukherjee13946 жыл бұрын

    Very nice

  • @Ale93Difo
    @Ale93Difo4 жыл бұрын

    You made my day

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you

  • @nada.h.majeed
    @nada.h.majeed3 жыл бұрын

    Bigger thanks for you Gad bless you 🙏🙏🙏🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @dstan16224
    @dstan162244 жыл бұрын

    Sir...If Dicer has endonuclease activity then why is it chopping off the siRNA from the sides as 9:30? Please explain it Sir. Thank you.

  • @esmeraldasish
    @esmeraldasish7 жыл бұрын

    Cloud you please do videos on MLS 4550 Clinical Immunohematology?

  • @ozgunteksoy4398
    @ozgunteksoy43984 жыл бұрын

    thank you for your trainer videos. I want to shoot video about siRNA designing on a specific example

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @beech9443
    @beech94438 жыл бұрын

    should it actually be that coding and non-coding strand of DNA is being transcribed, so RNA can base pair not transcription of RNA?

  • @peacemorepeace2000
    @peacemorepeace20009 жыл бұрын

    very nice

  • @mohammadkhashan7425
    @mohammadkhashan74253 жыл бұрын

    Thank you very much, you are amazing. I have been following your tutorials for a long time. But in this video I think there is a mistake, you said RISC is constructed of slicer and Argonaut, this is a mistake because the slicer is Argonaut, but other parts are components like GW182.

  • @dondapatisrujan8351
    @dondapatisrujan83513 жыл бұрын

    Very nice presentation

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @darshanjain7113
    @darshanjain71136 жыл бұрын

    Which strand remained attached to the RISC complex if it would of been the antisense one then and only then we get an inhibition effect of target mRNA.. what if that strand is a sense strand.. then the whole process will be futile?.. plz answer the query.. it was a helpful video though..

  • @ravleenpannu1573
    @ravleenpannu15735 жыл бұрын

    Amazing explanation 👏🏻

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you

  • @aiswaryaganga7646
    @aiswaryaganga76463 жыл бұрын

    Sir whether the double stranded rna is produced from the target sequence and it will cleave the same target mrna

  • @shrutireddy6151
    @shrutireddy61515 жыл бұрын

    what about the guide rna strand?? after cleaving the target rna strand what happens to the guide rna

  • @vishnudasan3711
    @vishnudasan37117 жыл бұрын

    bro you say that in miRNA after forming RISC there are two domains one is paz that attach with single stranded mRNA & another is pivi domain have RNase activity..OK in all type of so,sh,mi RNA strands are unwinds by helicase?? are some factors involved??

  • @anuhyavalluri4881
    @anuhyavalluri48813 жыл бұрын

    Hi Suman sir...I feel the extreme joy of learning while watching your videos....Can you please make a video on RNA i antagonist process .....how the plant viruses suppress the RNA i ....

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you. I will do that in a separate video

  • @anuhyavalluri4881

    @anuhyavalluri4881

    3 жыл бұрын

    Thank you sir....your efforts are much appreciated...

  • @akashmishra1285
    @akashmishra12854 жыл бұрын

    What are the intranuclear processing of a ds rna before leaving the nucleus??

  • @affbhaskar
    @affbhaskar Жыл бұрын

    No words to express 🤗

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Thank you

  • @arpanchakrabarty2464
    @arpanchakrabarty24647 жыл бұрын

    fabulous sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +Arpan Chakrabarty you're welcome

  • @akanshasingh1746
    @akanshasingh17468 жыл бұрын

    sir most of the plant viruses are single standed so what is the use of rna silencing in case of plant-virus system

  • @halimakhatoon6722
    @halimakhatoon67224 жыл бұрын

    Sir does dsrna produce when the plant get infected with virus or its present already bin plant???

  • @pramodkudash2817
    @pramodkudash28174 жыл бұрын

    Sir, can u tell me if this can work in acellular organisms

  • @saidharani6731
    @saidharani67314 жыл бұрын

    Excellent sir tq sooo much 🙏🙏🙏🙏🙏🙏🙏🙏🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @Light_in_Dark00
    @Light_in_Dark00 Жыл бұрын

    No doubt great teacher you are😊..but plz make it clear that..only siRNA need NPC complex for its transportation (from nucleus to cytoplasm) or miRNA and shRNA also need????

  • @dr.balasubramanianv3437
    @dr.balasubramanianv34375 жыл бұрын

    What will happen to the passenger strand? whether its get degraded or not?

  • @nutribox4535
    @nutribox45355 жыл бұрын

    my overseas professor of all time

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you

  • @snehajoby3150
    @snehajoby31504 жыл бұрын

    Once I had dumped this rna interference part coz it was so hard..but now I understood everything and its easy..thanku so much sir..u are really awesome.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @maryshelly6163
    @maryshelly61633 жыл бұрын

    very clear

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @shaz781
    @shaz7813 жыл бұрын

    You are outstandingggggg. Love from Pakistan👍subscribed.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

  • @amruthamavily
    @amruthamavily6 жыл бұрын

    9.23 endonuclease...how??dicer is cutting from the end...then how?

  • @varsharamachandran4043
    @varsharamachandran40434 жыл бұрын

    RNase III is a endonuclease enzyme and how it able to cut in the sides or in the end of the strands ?????

  • @muslihakamarudheen9800
    @muslihakamarudheen98002 ай бұрын

    Sir, which books are u referred ? Plz tell me sir....it will help for my research work

  • @n-sh2014
    @n-sh20143 жыл бұрын

    Many thanks for the video. Very informative as usual. However, if I'm not wrong, there is a mistake. In the RNAi introductory video, you say Dicer is RNase H, but you say Argonaut is RNase H in this video.

  • @vishnudasan3711
    @vishnudasan37117 жыл бұрын

    dicer(RNase III) cleave ds RNA in pre mi RNA in cytoplasm and drasha cleave mi RNA in pri mi RNA form..

  • @peacemorepeace2000
    @peacemorepeace20009 жыл бұрын

    have u cleared net jrf but very nicely and intelligently done

  • @rameshv8429
    @rameshv84295 жыл бұрын

    Amazing explanation about interference mechanism thanks sir

  • @ArivomThelivom
    @ArivomThelivom5 жыл бұрын

    How double stranded Rna is introduced

  • @wycliffenyandika9017
    @wycliffenyandika9017 Жыл бұрын

    nice!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Thank you

  • @vijayanagpurkar3875
    @vijayanagpurkar38754 жыл бұрын

    If so rna block the expression of m-RNA then how normal translational takes place

  • @dhananjaymishra6657
    @dhananjaymishra66575 жыл бұрын

    How we send DS RNA into plant

  • @savitakamble4854
    @savitakamble48546 жыл бұрын

    very useful thnks

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    +sau Kamble you're welcome

  • @savitakamble4854

    @savitakamble4854

    6 жыл бұрын

  • @bharatnagesh9859
    @bharatnagesh98596 жыл бұрын

    RISC , (RNA induce silencing complex) not RNA interfering silencing complex

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    Yes. Thanks for the correction.

  • @Megha-ij8eo

    @Megha-ij8eo

    3 жыл бұрын

    Yes...u r correct...its RNA induced silencing complex...👍

  • @selvamselvam1225
    @selvamselvam12254 жыл бұрын

    This method use in Corona disease ??????

  • @sagarmovaliya9006
    @sagarmovaliya90065 жыл бұрын

    One requesting for make pi or Piwi rna

  • @ritikasingh8809
    @ritikasingh88093 жыл бұрын

    After cleaving the mRNA , whats the fate of guide strand further??