The lipids are removed with using a solution of a hydrogel monomer and formaldehyde solution as the first step. Heat is then added to the mix so that the hydrogel monomers are able to polymerize therefore keeping the integrity of the structure to the brain. Then, SDS (sodium dodecyl sulfate) is added to break the lipids apart and remove them from the brain. ETC (Electrophoretic Tissue Clearing) is then used t separate the lipids from the rest of the tissue based on charge/mass.

@bomchoi2536

4 жыл бұрын

Paul B Why are the neurons projected green? Arent they supposed to be transparent as well??

@TheClakki

2 жыл бұрын

@@bomchoi2536 perhaps it’s because, to make them visible (soma and axon), they used specific radioactive tracer techniques (in this case the color of the substance used was green), I suppose.

@bomchoi2536

2 жыл бұрын

@@TheClakki oh thanks!! Are you studying this field?? I forgot about this comment!

@TheClakki

2 жыл бұрын

@@bomchoi2536 Yeah, in some way. I’m studying neuroscience and psychobiology, that’s why I know a little on this field and techniques.

@Dragon-Slay3r

Жыл бұрын

Nice

This is by far one of the coolest things science has accomplished!

Clarity deserves nobel prize in 2014

@Muuip

8 жыл бұрын

I agree.

@iceman4382

6 жыл бұрын

Agreed

This is the sexiest thing I've ever seen on youtube.

Amazing research. We are living in an exciting time.

this definitely shows a much more detailed look into the mystery of our own bodies.

Thank you Karl Deisseroth for this huge leap in neuroscience!! You are simply a genius and everyone at the University of Toronto neuroscience program is going nuts about the new paper/video. CLARITY is breathtaking

@Dragon-Slay3r

Жыл бұрын

Nice

Beautiful. Simply beautiful.

This is absolutely incredible.

I can see clearly now....the brain has gone ;0)

@Muuip

8 жыл бұрын

ok that was funny!

@achimaufachse5925

6 жыл бұрын

i love the reference...my wife is just rolling her eyes over my stupid humor...

@shypsy4852

5 жыл бұрын

Haha

I can see clearly now, the brain has "gone", it's gonna be a bright, bright sunshiny day

Definitely needed to see this in the middle of grueling exam week- reminds me that it's all gonna be worth it at the end :)

@McPhysX

4 ай бұрын

what are you now?

We have the capacity to generate prescient ideas, we have the hypothesis, we have the theorems, but we don't have the means to test, observe or quantify many of them..... until now. Techniques like this allow us to blow apart ambiguity, expose fact and tap in to rich, glorious knowledge.

excellent. Some clearing agents have been going around these past years, but the clearing speed and resolution are superior. congratulations.

This is a great tool for research. Best visualization sofar, along with flash and freeze electron microscopy.

That was absolutely awesome and beautiful to watch. Well Done Ladies and Gentlemen. Well done.

I don't understand why did three people dislike this?? please explain!! As for me I give it a "SUPERLIKE"

wow, extremely impressive! one easily recognizes the frame and scale bar of the imaris software, rendering so fast and smoothly.

Absolutely amazing. Although It's a shame we can't get this sort of visualisation on brain tissue that is alive. Well maybe one day...

It's spectacular neuroscience. I am very fascinated!

Best visualization of the brain I have seen!!!

If you can peer into my mind with that technology you'll never quit me

Amazing work...The brain visualisation in this video were created using 'Imaris' software from Bitplane.

This is stellar. I am 34, what we had 20 years ago to now is absolutely staggering. This is so amazing to be able to see the brain. How flipantly they just kind of point out "Oh this may be one of the major symptoms of Autism" is amazing. Imagine; for a second, where this will be when combined with other mapping and man/machine interfaces in say 20 years. I am just blown away at the casualness of these discoveries now. Wow. Just wow

This is simply... Amazing!

This is fascinating, and exciting, this is science, and art, and that's why I love it so much. Thanks a lot for sharing this!

Fascinating! this makes me think of the work that's being done isolating the collagen structure of organs and re-growing tissues over them - they look similarly transparent in the 'scaffolding' stage. But this - I never dreamed this was possible! Truly amazing work.

Deisseroth has done it again! Kudos!

It is really amazing how we visualize the miracle of nature

It is a see-through brain, watch again (at 1:33) for the mouse's brain that had been treated. All the fat had been removed and all that was left was grey matter suspended in hydrogel. You could actually look through to read the printed text on the sheet underneath the brain. The colourful graphics just illustrate the markers used to see the three dimensional structures of the brain.

Inspiring and fascinating!

This will get us to the next level for sure!

Thanks Hilda cerdeira:)

So freaking Amazing! I had dreams that this would eventually happen. I just want to go back to school and focus on neuroscience.

Mind blowing (literally)

Increíble. Me encanta haber nacido en esta época.

That was astonishing.

well done! amazing idea

My youngest son (4) has autism. It is my hope that science such as this will help us to one day better understand why autism develops, and perhaps how it can be avoided, or even shed light on how to help these individuals learn in their own unique ways to live independent lives.

cool cool cool, can't wait to see this being used on other organs, and in combo with organ printing.

"The brain is a world consisting of a number of unexplored continents and great stretches of unknown territory." Santiago Ramón y Cajal a.k.a. neuroscience legend/ bawse

When I go, I might leave my brain so that scientists and doctors might be able to do the same thing with mine.

This makes me happy

Extraordinary!!!

This is beautyful! If it can be extended to living and operating organisms not only the structure but also the processing synapsis could be observed in the whole system.

I guess you could say... *puts on black sunglasses* They were reading his mind. YEAAAAAAAAAAAAAAAAAAAAAAAAH

This is a gamechanger.

This is freakin nutz

HOLY COW! I mean, MOUSE! And I missed this by... 9 years?? How come all the newer brain videos don't show clarity such as this? (pun intended)

Brilliant!

this soundtrack is so lit I can't even pay attention

and where do they us these tools? What if I have a very complex neurological illness that all current tests fail to detect. Where would I find someone with this?

Exciting result!

If you can replace the fat with a different insulating but see-through material without disrupting connections... Highly unlikely. However, this technique used in conjunction with optogenetics (i.e. after optogenetics experiments performed, use clarity to acquire detailed map) has very exciting possibilities!

So amazing.

Future implications for such a technology are grand.

From what I've heard this can be accomplished on live subjects as well enabling them to see thoughts and brain activity as it happens.

@MrBenIPresume

9 жыл бұрын

AuSands1 Absolutely impossible on live subjects. This technique replaces the cell's membrane with a gel. Removal of the membrane completely destroys any chance for the cell's survival.

@AuSands1

8 жыл бұрын

Alyss Flynn Thanks!!!

Amazing!

Fantastic!

it is awesome what this world is reaching in technology

One step closer to understanding the human brain :)

That's awesome imagery of the #humanbrain and a rat brain - impressive resolution, clarity, and quality. #BrainResearch .

I totally agree with you on the 'in vivo' issue. But I am also optimistic that the technique will drastically improve 10 years down the line.

@santimezzano393

Жыл бұрын

did it?

Mind-blowing. :)

I vote for your SUPERLIKE REALLY FANTASTIC

AMAZING!!!!!

wonderful technology! I wonder how they manage to keep the structure of the brain and neural connections from destruction, incl. from decomposition And how well do you manage to keep the structure?

If you're a college student then your library almost certainly has a subscription to Nature. Just ask your librarian how to access it, a portion of you tuition goes towards paying for such subscriptions. You probably can do it through your library website without even leaving your chair.

I am looking for the paper where it is being published with the supplementary material, Can someone help me here?

stunning

Pretty kewl,,,,,as long as it's used for good.

Absolutely amazing. E-brains here we come!

Amazing. Hope they try the same with the heart

I don't know what to call this other than: Fucking. Amazing.

So mapping the brain slice by slice was a waste of time? That's a relief.

wonder ful

well done

I'm waiting for such quality for living brain tissue! Awe-some!

This reminds me of the book Invisible Man by H.G Wells. The idea is entertaining.

Does anyone know what music is playing in the background?

so if our cells didn't have membranes made of fat we would be transparent?

While this looks really neat, most of the things mentioned in this video have been possible decades ago with simple histological smears.

Can't believe only 30 poeple recently had a paper rejected in Nature!

AMAZING!!! but still remain structure↔function correlation problems and emergent properties of the MIND...

While it's not possible to do this in living tissues, what you can do is study the components of the organ, trying to understand "what went wrong" there. What the technique will do is revolutionize the way we study brain pathways related pathology

Hence the word 'potentially'!

who else got goosebumps watching the brainstem like never before..?

how the sweet flying fuck can someone dislike this video? one of the most exciting videos i've seen in years! GO SCIENCE!

I love science

so rock n roll !

This is a huge step.

WOW!

you can get the paper on the website of Karl Deisseroth one of the authors of Stanford University. I can not put here because youtube will not let me put Internet sites.

Hell yeah

FANTASTIC Science rocks

Yes, definitely - it's very invasive. To image a living brain, one needs a scanner of some type, like an MRI or PET scanner. MRI has better resolution than PET, but neither has the type of resolution this technique can achieve.

This isn't a see-through brain - this is how to see through a brain (using clever imaging techniques). Very different things.

Your college library. Most large public libraries will also have a subscription to Nature.

That is cool.

am dizzy imagining all the sensory information that can quickly traverse through the brain.