GREAT video. Thanks.

Today I have 90 samples prepared for targeted metabolomic assay. thank you.

superb, really awsome and informative lecture

Thanks!

super. Thank you

Thankyou 😊

Please what did you use to aspirate? Or can you please explain the aspiration part?

Please can you explain the quenching procedure? Aspiration was done . What was used for aspiration? Am currently working on extraction of metabolites from Enterococcus species and I need more guides

@shakirshamas6286

Жыл бұрын

same question and same procedure and guidance I needed. i am working on primary metabolites of chickpeas

I put my cell lysate in -80. Can I use them for metabolomic? Too late to add methanol to quench metabolism?

Hi, Thanks for this video. Please I have 2 questions: 1- I why you stored the extraction solution for 1 hour before use? 2- What is the red solution? Is it bacteria cells with its extracts? Could you please please tell me more about the bacteria cells sources , preparation , how you stimulate them to excrete secondary metabolite? Thanks

@EMBLorg

3 жыл бұрын

Information about the protocols can be found here: www.embl.de/mcf/metabolomics-core-facility/protocols/index.html including contact details for the EMBL Metabolomics Core Facility, if you have further questions.

@PrasadPhapale

3 жыл бұрын

Hi Aziz, 1) the storage is required to have cold methanol for extraction. The cold methanol is necessary for quenching the metabolism before extraction 2) This protocol is for adherent mammalian cells; the red color is from DMEM medium in which cell are grown.

hi, can I get a softcopy of this protocol of metabolite extraction for my final year project? Thank you.

@EMBLorg

4 жыл бұрын

Please contact the Metabolomics Core Facility team, clearly stating the tutorial you are referring to and what you would like them to send you. You can contact them through the EMBL website: www.embl.de/mcf/metabolomics-core-facility/contact/index.html

@PrasadPhapale

3 жыл бұрын

Hi Jennifer, soft copy is available on our webpage under protocols. Thanks!

@jarrysc

3 жыл бұрын

Hi, Jenn, Did you find the protocols?, can we speak in a private conversation by mail... javiersanchezing@gmail.com, will write whoever worked with extraction of metabolites... I'm really trying to get the way to extract from Trichoderma sp. and I have actually lot of questions about.

Here I can't find the subtitles too, can you help us please.

@EMBLorg

3 жыл бұрын

There are no subtitles, because there is no speaking in the video, it is just background music.

Sir I want extract metabolite from actinobacteria and Its not yielding good amount of extractant

@swathireddy8807

3 жыл бұрын

And I'm following protocol from one of the article and they will say to repeat the extraction 3 times should I do this

@abhijitmandal3245

Жыл бұрын

if you read my reply to your mail kindly response . i am working on antibiotic producing bacillus from . can you help me on this? ... from India

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