RNA interference (RNAi) Animation || miRNA || siRNA || mRNA regulation

Фильм және анимация

#biologyanimation #RNAinterference #siRNA #miRNA
This animation describes the history and discovery of RNAi along with a detailed gene silencing mechanism by mi RNA and si RNA. This also describes the processing of miRNA. Different mechanisms of mRNA
regulations are mentions and explained in this animation.
Gene silencing mechanisms of siRNA and miRNA.
siRNA: dsRNA (either transcribed or artificially introduced) is processed by Dicer into siRNA which is loaded into the RISC. AGO2, which is a component of RISC, cleaves the passenger strand of siRNA. The guide strand then guides the active RISC to the target mRNA. The full complementary binding between the guide strand of siRNA and the target mRNA leads to the cleavage of mRNA.
miRNA: Transcription of miRNA gene is carried out by RNA polymerase II in the nucleus to give pri-miRNA, which is then cleaved by Drosha to form pre-miRNA. The pre-miRNA is transported by Exportin 5 to the cytoplasm where it is processed by Dicer into miRNA. The miRNA is loaded into the RISC where the passenger strand is discarded, and the miRISC is guided by the remaining guide strand to the target mRNA through partially complementary binding. The target mRNA is inhibited via translational repression, degradation or cleavage.

Пікірлер: 48

  • @KevinBolsajian
    @KevinBolsajian2 жыл бұрын

    I watched at least 15 videos but nobody explained it as clear as you did. Thank you so much🙏🏻

  • @RethinkBiology

    @RethinkBiology

    2 жыл бұрын

    Thank you so so much 🥰 keep supporting

  • @vihaansworld9442

    @vihaansworld9442

    Жыл бұрын

    Same here mam

  • @rakshitasingh6574
    @rakshitasingh65747 ай бұрын

    You don't know how much it helped me ... THANKYOU SO MUCH 🙏🏻🙏🏻 .. such a clear explanation

  • @prernaghangas2197
    @prernaghangas2197 Жыл бұрын

    Absolutely amazing. Thankyou 🙂

  • @ibrahimmicheal7473
    @ibrahimmicheal74736 күн бұрын

    This is quite simple....thanks much!

  • @RethinkBiology

    @RethinkBiology

    3 күн бұрын

    Glad it was helpful!

  • @ikinehir
    @ikinehir6 ай бұрын

    Thank you, thank you!

  • @saykar0393
    @saykar03933 жыл бұрын

    Great.... !

  • @katiek9361
    @katiek9361 Жыл бұрын

    literally slay thank u so much

  • @neeloferjan5397
    @neeloferjan53976 ай бұрын

    Amazing 🤩,thanku so much

  • @mirzafarhanghazi
    @mirzafarhanghazi2 жыл бұрын

    It was the best and simplest explaiination of RNAi , thank you 😊

  • @RethinkBiology

    @RethinkBiology

    2 жыл бұрын

    That's a great honour

  • @ayumentari2091
    @ayumentari20912 жыл бұрын

    The best explanation video ever, so clear and complete! Thank you so muchh 🙏🙏🙏

  • @RethinkBiology

    @RethinkBiology

    2 жыл бұрын

    Thanks a lot to you too dear 😊

  • @AjaySingh-fh8bz
    @AjaySingh-fh8bz2 жыл бұрын

    very nice

  • @adnankhanthebest
    @adnankhanthebest3 жыл бұрын

    Your way of explaining the concepts is very good.

  • @RethinkBiology

    @RethinkBiology

    3 жыл бұрын

    Thanks a lot

  • @laraibsanjrani1523
    @laraibsanjrani1523 Жыл бұрын

    Great, I really appreciate it. You explained it very well 👍🏻

  • @RethinkBiology

    @RethinkBiology

    Жыл бұрын

    Glad you liked it

  • @muhammadumarjogezai7353
    @muhammadumarjogezai7353 Жыл бұрын

    Best way of explanation 👍 Thank you so much 😇

  • @RethinkBiology

    @RethinkBiology

    Жыл бұрын

    Thank you! Keep supporting!

  • @vladimirputin965
    @vladimirputin96510 күн бұрын

    Thank you❤

  • @RethinkBiology

    @RethinkBiology

    3 күн бұрын

    You're welcome 😊

  • @DemetriMaude
    @DemetriMaude8 ай бұрын

    good video :)

  • @ashishdangi7946
    @ashishdangi79462 жыл бұрын

    Thanks for your efforts👏👏👏

  • @RethinkBiology

    @RethinkBiology

    2 жыл бұрын

    Thank you 😊

  • @schoolbioresearch9853
    @schoolbioresearch98537 ай бұрын

    Tq so much , I enjoyed watching this 😍😍❤❤

  • @RethinkBiology

    @RethinkBiology

    7 ай бұрын

    So glad!

  • @punm1983
    @punm19832 жыл бұрын

    Superb

  • @RethinkBiology

    @RethinkBiology

    2 жыл бұрын

    Thanks a lot, please share if you liked our videos

  • @vladimirputin965
    @vladimirputin96510 күн бұрын

    The information is from which book?

  • @pramods3630
    @pramods36306 ай бұрын

    Mam I have a doubt in which condition miRNA and siRNA will gets activated

  • @user-tu5bf6cm2j
    @user-tu5bf6cm2j6 ай бұрын

    عاش اميتاب باتشان و الله تاليها كمنا نفهم على الهنود 😅

  • @joshualaraclure4863
    @joshualaraclure48637 ай бұрын

    Isn't AGO1 for miRNA ?

  • @ayuchaudhary4135
    @ayuchaudhary41352 жыл бұрын

    Hey can you tell me about RITS comples

  • @RethinkBiology

    @RethinkBiology

    2 жыл бұрын

    The RITS (RNA-induced initiation of transcriptional gene silencing) complex contains siRNAs and is the major effector of RNA interference. Heterochromatin assembly is associated with large-scale chromatin condensation to regulate a variety of chromosomal processes, such as centromere formation. Below is the link for a Cell article, you may find useful. We will try to make a video on that too. www.cell.com/molecular-cell/pdf/S1097-2765(04)00057-7.pdf

  • @ayuchaudhary4135

    @ayuchaudhary4135

    2 жыл бұрын

    @@RethinkBiology thanks

  • @nandani6945
    @nandani694510 ай бұрын

    How to apply in plant.. ?

  • @RethinkBiology

    @RethinkBiology

    10 ай бұрын

    RNA interference is a natural process that plants use to regulate gene expression and defend against viruses and other pathogens. It involves the production of small RNA molecules that bind to specific messenger RNA (mRNA) targets and prevent them from being translated into proteins. There are different types of small RNAs, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), that have different origins and functions in plants. To apply RNA interference in plants, researchers can use various methods to introduce small RNAs or their precursors into plant cells. For example, they can use genetic engineering to insert DNA sequences that encode for small RNAs or hairpin RNAs (hpRNAs) into the plant genome. These sequences can be driven by constitutive or inducible promoters to produce small RNAs in a specific tissue or time. Alternatively, they can use viral vectors to deliver small RNAs or hpRNAs into plant cells through infection. These vectors can be modified to reduce their pathogenicity and increase their specificity. Another method is to use spray-induced gene silencing (SIGS), which involves spraying synthetic small RNAs or hpRNAs onto plant leaves. These molecules can enter the plant cells through natural openings or wounds and trigger RNA interference. The applications of RNA interference in plants are diverse and promising. RNA interference can be used to study gene function, improve crop traits, enhance stress tolerance, and control pests and diseases. For example, RNA interference can be used to silence genes that are involved in flowering, fruit ripening, seed development, nutrient uptake, photosynthesis, and hormone signaling. This can help researchers understand the roles of these genes and create plants with desirable characteristics. RNA interference can also be used to silence genes that are essential for the survival or virulence of plant pathogens, such as viruses, bacteria, fungi, nematodes, and insects. This can help plants resist infections and reduce crop losses. If you want to learn more about RNA interference in plants, you can check out these sources: (1) RNA Interference Technology in Plants: Mechanisms and ... - Springer. link.springer.com/chapter/10.1007/978-981-16-6974-3_10. (2) RNA Interference Past and Future Applications in Plants - MDPI. www.mdpi.com/1422-0067/24/11/9755. (3) RNA Interference- Definition, Mechanism & Applications - BYJU'S. byjus.com/biology/rna-interference/. (4) Exploiting RNA Interference Mechanism in Plants for Disease ... - Springer. link.springer.com/chapter/10.1007/978-3-030-35955-3_10. (5) RNAi: What is its position in agriculture? | Journal of Pest Science. link.springer.com/article/10.1007/s10340-020-01238-2.

  • @srijandey87
    @srijandey873 ай бұрын

    Are you bengali?

  • @merajbegum5978
    @merajbegum59784 ай бұрын

    Better not to add music!

  • @RethinkBiology

    @RethinkBiology

    3 ай бұрын

    See our latest videos, we don't add music 😬

  • @NITISHKUMAR-un2no
    @NITISHKUMAR-un2no Жыл бұрын

    Background music is disgusting

  • @RethinkBiology

    @RethinkBiology

    Жыл бұрын

    Visit our newest videos with no background music

  • @warmedpuma2024

    @warmedpuma2024

    11 ай бұрын

    It was a good choice of music, so be quiet jit.

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