Recombinant DNA technology | DNA Vectors | Cloning Vector And Expression Vector

This recombinant DNA technology lecture explains about different types of DNA vectors such as cloning vector and expression vector. This lecture explains about the basic features of cloning vector and expression vectors and the difference between cloning and expression vector. In this video I also talk about different examples of cloning vector such as DNA plasmid, phagemid, cosmid, BAC, YAC and so on.
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    3 жыл бұрын

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    3 жыл бұрын

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    3 жыл бұрын

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    @jamessanga8945

    3 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    That's a huge complement. Thank you for watching the lecture

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    @violetgirl615

    5 жыл бұрын

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    5 жыл бұрын

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    7 жыл бұрын

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    @Contentcreator156

    6 жыл бұрын

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    @taquiimam5780

    6 жыл бұрын

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    @taquiimam5780

    6 жыл бұрын

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    6 жыл бұрын

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    @shomusbiologyofficial

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    @shomusbiologyofficial

    Жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

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    @shomusbiologyofficial

    Жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

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    4 жыл бұрын

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    @shomusbiologyofficial

    7 жыл бұрын

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    @shomusbiologyofficial

    6 жыл бұрын

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    @sabreena.s8580

    4 жыл бұрын

    Hi

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    @Jay-lq8ly3 жыл бұрын

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    @inshaamin15256 жыл бұрын

    vivid and simply best video to understand cloning.... thank you

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you for watching the lecture

  • @bightonchinembiri
    @bightonchinembiri7 жыл бұрын

    Been using your videos since my freshman year, and always you explain well. all the gratitude from ZImbabwe

  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +Bighton Chinembiri thank you very much. Glad you liked my lectures

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    @akritivishwakarma73125 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you for appreciating my efforts

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    @ajaysinghjaral6 жыл бұрын

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    @shomusbiologyofficial

    3 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

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    @sheikhnaseer46376 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 ай бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

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    @FOODIESWITHAQSA2 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +Alwina Anam thank you.

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    @mechikaboombayah4960

    7 жыл бұрын

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    4 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

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    @27zothansanga637 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +sanga ATLAS you're welcome

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    @Livecanada20246 жыл бұрын

    Very helpful lecture sir ... thq..may Allah reward you with goodness

  • @graciousmayesi4431
    @graciousmayesi44315 жыл бұрын

    Well explained with patience, well done Shomu.I hardly understood the concepts of DNA Recombinant Technology. Thank you.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you for appreciating my efforts

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    @mathswithcorra7 жыл бұрын

    what a legend! such a logical approach. Thanks from S.A

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    @shomusbiologyofficial

    7 жыл бұрын

    +Corne Kriek thank you. Glad it helped

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    @promisendlovu85508 жыл бұрын

    lots of love from South Africa

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    @kopanokhumalo4344

    4 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you for appreciating my efforts

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    @shalinic70527 жыл бұрын

    nice tutorial very useful to me well teaching capacity u have amazing it was

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    @martinahejnickova30908 жыл бұрын

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    @meghanach86146 жыл бұрын

    Awesome teaching!! Plz continue it......

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    @sciencegeek27912 жыл бұрын

    Excited to watch..explaining the idea in simple terms to understand 🤩🤩

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts

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    @pin24119948 жыл бұрын

    jabadast explain karo chho tame...

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    @subhiksharajan85475 жыл бұрын

    I've passed all 5 semesters till now , its bcos of u only sir thank you so much

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you for watching the lecture

  • @arunakapalinee3623
    @arunakapalinee36233 жыл бұрын

    You made this topic very easy.... Thankyou very much sir🙏

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    @shomusbiologyofficial

    5 жыл бұрын

    Thank you so much for appreciating my efforts

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    @golapemadina71775 жыл бұрын

    You are great !!!sir 🙏 thank you for making this topic so much easier.

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    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

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    @shomusbiologyofficial

    4 жыл бұрын

    Glad to hear that you're getting benefit from my lectures

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    @jamespaul32767 жыл бұрын

    Awesome lecture, thank you

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    @rimpikhanra54874 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

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    @bsurve111 ай бұрын

    Thank you very much for putting these videos, helped me a lot for basic understanding. ❤

  • @shomusbiologyofficial

    @shomusbiologyofficial

    11 ай бұрын

    You're welcome

  • @yelizergol5070
    @yelizergol50703 жыл бұрын

    Wish you were my Rec DNA Tech professor Mr. Shomu !

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you. You can always watch my KZread lectures

  • @dinkerkumar4325
    @dinkerkumar43256 жыл бұрын

    These topics smartly explained

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    @shyamghodasara50734 жыл бұрын

    Seriously Sir, You are a Life saver for each and every biotechnology students thank you so much

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you so much for appreciating my efforts

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    @sarahalshair20516 жыл бұрын

    Thanks for the informative video.

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    @buyisilemazibuko16665 жыл бұрын

    you are a life saver man, thank you

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    @SoudaAlvi3 жыл бұрын

    Sir g you r the best best best.. I always watch ur video before exam. Nd it’s so informative 😍😍😍plus easily and perfectly explained.

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    @thulasirajeshkumar28777 жыл бұрын

    your videos helped a lot thank you sir

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    @khaleelzainel25436 жыл бұрын

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    @albertyumnam41712 жыл бұрын

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome. Glad you like it

  • @nouram4151
    @nouram41512 жыл бұрын

    Thank you Sir. You should be a professor at our uni. ❤️ great explanation

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @ayushshah7582
    @ayushshah75828 жыл бұрын

    Perfect....Hatsoff...

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    @BAMS584345 жыл бұрын

    Your videos clear all the confusions

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

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  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you. Glad you liked my lectures

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    @wedadmohammad59386 жыл бұрын

    السلام عليكم. .جزاك الله كل خير

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    @tayyibasaleem25284 жыл бұрын

    Shomu sr u r bestest teachr in the world...☺

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you

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    @tayyibasaleem2528

    4 жыл бұрын

    @@shomusbiologyofficial My pleasure...☺

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    @deepakkumarnayak63415 жыл бұрын

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    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

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    @deepakkumarnayak6341

    5 жыл бұрын

    yes sir u r really owsm

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    @amitdhyani51564 жыл бұрын

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    @shomusbiologyofficial

    4 жыл бұрын

    Glad to hear that you're getting benefit from my lectures

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    @sakshisharma67575 жыл бұрын

    thankyou so much its my rdna exam today and your videos are helping me too much ......thanks alot sir

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    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

  • @randaalissa905
    @randaalissa9054 жыл бұрын

    What an amazing explanation, thank you so much🌺

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

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    @kanwalilyas44974 жыл бұрын

    sir thanks ur videos are really ammazing and full of knowledge God bless u for this goodness

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you

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    @jasmingorasiya35094 жыл бұрын

    Very very good...You are the best teacher Of biology...God bless you..

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

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    @raginiprabakar80077 жыл бұрын

    really very very helpful

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    @sivanihostel53337 жыл бұрын

    very good to explain that the following document

  • @alwinaanam1231
    @alwinaanam12315 жыл бұрын

    U helped me a lot in understanding lifescience.... Thank you 😇

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @fauziasultana1226
    @fauziasultana12262 жыл бұрын

    U r best .....feel comfortable in learning.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @rahulganesh468
    @rahulganesh4684 жыл бұрын

    Thanks for the help! Very informative video!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @abassanifowoshe9920
    @abassanifowoshe99204 жыл бұрын

    Great teacher. very helpful. Thank you

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @jadehuynh2378
    @jadehuynh23784 жыл бұрын

    Thank you for the video. However the explanation about the selectable marker is not quite tight. I am referring to the section from minute 11:00 to minute 13:42. I might have misunderstood you. The selection of clone having the recombinant plasmid cannot be explained here. The bigger use for Selectable Marker region is for a different issue: the transformation might not be 100% efficient and bacteria might or might not have the recombinant vector. Before transformation, you have to separate this mixture to obtain a solution that contains only the recombinant vector, which is tranformed to the bacteria (if you have a mixture of both self-annealed vector and recombinant vector to be transformed to bacteria, every bacteria that has either vector will grow in the antibiotic). Whichever bacteria does not have the vector will not grow, whichever bacteria does have the vector will grow, and of course it will have our interested gene. Just something for clarification!

  • @Rizek234

    @Rizek234

    Жыл бұрын

    Yeah, unfortunately Somu got this part wrong. I actually was watching this video just to learn about ways to prevent self-ligation of the vector. So far, I know two ways to prevent it. 1) In the ligation reaction, increase the concentration of the insert compared to the vector, like 6:1 or even higher. This increases the propability of vectors ligating with the insert. However, self-ligation is still possible. 2) Treat your vector before the ligation with Alkaline phosphatase. This enzyme will phosphorylate the ends of the (already digested) vector. Phosphate groups are negatively charged and repell each other, thus preventing contact of the vector ends with each other. In any way, to identify if the insert is correctly integrated, an analytic digest is necessary (using restriction enzymes upstream and downstream of the insertion site).

  • @mahathivenkatraman4892

    @mahathivenkatraman4892

    Жыл бұрын

    I was looking for someone to point this out and you did it, thanks a million!

  • @hassanal-rubaie5933
    @hassanal-rubaie59337 жыл бұрын

    "Thanks you" is a little for you . You are from the few beautiful things in world . If you have a video that explains yeast cloning vector please give me the link 😘😘😘

  • @pseudomonas69
    @pseudomonas696 жыл бұрын

    bruh like who ARE YOU and why are you so goddamn smart and on your shit. youre the best

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    +Sara Alexander thank you. Glad you liked my lectures

  • @creativeartfreya1867
    @creativeartfreya18673 жыл бұрын

    Brother u r genius ......u r teaching fabulous....no word cant express your teaching method....

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @khadeejasalah776
    @khadeejasalah7762 жыл бұрын

    Sir donno how to express my gratitude nd love towards you you are my best teacher

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @zooupsc
    @zooupsc3 жыл бұрын

    Got around 82 percent in undergrad . By relying 80 percent on ya videos

  • @AwaisAkram7777
    @AwaisAkram77772 жыл бұрын

    Love from Pakistan...... Your, all the lectures are very informative. Keep it up...

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts