I loved the spooky sound in between 😂😂😂😂

@quanhuiye1489

4 жыл бұрын

so do I

@urmeniamu

3 жыл бұрын

Would have been spooked myself had I not read your comment before starting to watch the video :-D

thank you, Rebecca.

Great video, thank you

Great Demonstration ♥

Nice demo Rebecca!!

very helpful. thanks.

thanks rebeca

Hi Rebecca, thank you. I am curious how one can know how much wash to use if you are purifying a protein that cannot be seen like GFP? Is one column of wash good enough usually and then you can use the imidazole eluent? Thank you.

At the end, the chemical used for removing gfp from beads needed to be separated from the protein. I think that could have been mentioned as well.

Thanks!!!

i love this video

thks so much

Awesome!

@simonk.gyimah7273

7 жыл бұрын

Did Isaiah 46:9 say Jesus is God? Check your facts

May I know what is the method that you used to break the cell? how pure is the final product?

@veronicaviramontes8199

7 жыл бұрын

I am not sure what she used, but often sonication is used to lyse the cell. That's what I use for my lab anyways.

@anapitu72

7 жыл бұрын

yeah, sonication can be the answer or, for bigger volumes, you could use a French press (reverse system) method

Who can let me know how to determine agent for purification with curli in E Coli? :(

Hey Rebecca, Is that so called "cloudiness" a Biofilm that the Bacteria had produced ? If not, then what is it ? There has got to be a faster way to remove the GFP. lol Thank-You.

@nabarun83

4 жыл бұрын

It's the optical density/absorbance of the bacterial cells

I think that the GFP if I remember correctly

Hi, how did you isolated the proteins from the e.coli? which method?

@TheSergeyVlasenko

Жыл бұрын

By IMAC chromatography. In case you steel looking for the answer 7 years after you wrote it :)

All those collecting tubes should be placed on ice. Also, cell lysis was not shown, and protein extraction from lysate, before Nickel bead affinity chromatography was not even shown, but i guess whatever...

sister which protein do you want to purify?

no it's just pure biomass you are seeing, there are simply more bacteria than before.

Just subscribed, keep it goin. I'm trying to grow my KZread channel too, it's hard but it's still fun. Peace!

thank you its easy way for purification,, i need protocol for purify topoisomerase IV from pseudomonas aeruginosa. can you guide me. thank you

@veronicaviramontes8199

7 жыл бұрын

Do they have different polarities? If so maybe you could use column chromatography?

Where is the dialysis?

you did not even show people on how to use the sonicator or even vortexing the pellet with buffer.

gfp

how deleted my comments....... mo... f.....s!!, yeah I love this, so what, any problem? B-D!

@fgularte

9 жыл бұрын

Now I am happy again! thanks! :-D!

I love this girl, would you marry me? X-D!

@andrewareva4605

7 жыл бұрын

Nice try bud. She is mine.

@materioverda1655

4 жыл бұрын

She will become a nun

MONEY

i came here for protien purification but i dont care now , this girl s damn beautiful