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Probe the Western blot for your target proteins using primary and secondary antibodies
Learn more at www.piercenet.c....
Target proteins in a sample are detected by incubating the membrane with an antibody that specifically recognizes the protein of interest (called primary antibody). This antibody-target protein interaction is then detected either by conjugating the primary antibody with a detectable label, such as an enzyme conjugate, or by incubating the membrane with a labeled secondary antibody that only recognizes the primary antibody.
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Thank you. This is so simple, but its easy to get hung up on small details when your learning\doing it for the first time.
Thank you. This is so simple with your explanation and I"m going to use it in a competition for spaceIL.
@evyatarhamami834
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