PCR (Polymerase Chain Reaction)

Join The Amoeba Sisters as they explain the biotechnology technique PCR. This video goes into the basics of how PCR works as well as two examples of its potential use.
Table of Contents:
00:00 Intro
1:34 How does PCR work?
4:31 Why use PCR?
5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19)
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Factual References (and excellent for learning more detail about this topic):
Clark, Mary Ann, et al. “17.1 Biotechnology - Biology 2e | OpenStax.” Biology 2e, OpenStax, 28 Mar. 2018, openstax.org/books/biology-2e/pages/17-1-biotechnology
www.fda.gov/consumers/consume...
www.fda.gov/media/136151/down...
*General length of a primer in base pairs from NIH www.genome.gov/genetics-gloss...
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FURTHER READING SUGGESTIONS:
On RT-PCR for SARS-CoV-2:
www.ncbi.nlm.nih.gov/pmc/arti...
jamanetwork.com/journals/jama...
On Fluorescent Probes in PCR:
www.ncbi.nlm.nih.gov/pmc/arti...
www.ncbi.nlm.nih.gov/pmc/arti...
What about limitations or disadvantages of PCR?
www.ncbi.nlm.nih.gov/pmc/arti...
What about more uses for PCR (as well as more info about Nobel Prize):
www.nobelprize.org/prizes/che...
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The Amoeba Sisters videos demystify science with humor and relevance. The videos center on Pinky's certification and experience in teaching biology at the high school level. Amoeba Sisters videos only cover concepts that Pinky is certified to teach, and they focus on her specialty: secondary life science. Learn more about our videos here: www.amoebasisters.com/our-videos
Support Us? www.amoebasisters.com/support...
Our Resources:
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Handouts: www.amoebasisters.com/handout...
Comics: www.amoebasisters.com/paramec...
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Connect with us!
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TIPS FOR VIEWING EDU KZread VIDEOS:
Want to learn tips for viewing edu KZread videos including changing the speed, language, viewing the transcript, etc? www.amoebasisters.com/pinkys-...
MUSIC:
Our intro music designed and performed by Jeremiah Cheshire.
End music in this video is listed free to use/no attribution required from the KZread audio library kzread.info...
COMMUNITY:
We take pride in our AWESOME community, and we welcome feedback and discussion. However, please remember that this is an education channel. See KZread's community guidelines and how KZread handles comments that are reported by the community. We also reserve the right to remove comments.
TRANSLATIONS:
Thank you so much to our amazing translators!
Turkish subtitles by Kardelen Ünalleylioğlu
Hindi subtitles by Alisha Aggarwal
While we don't allow dubbing of our videos, we do gladly accept subtitle translations from our community. Some translated subtitles on our videos were translated by the community using KZread's community-contributed subtitle feature. After the feature was discontinued by KZread, we have another option for submitting translated subtitles here: www.amoebasisters.com/pinkys-... We want to thank our amazing community for the generosity of their time in continuing to create translated subtitles. If you have a concern about community contributed contributions, please contact us.

Пікірлер: 516

  • @AmoebaSisters
    @AmoebaSisters3 жыл бұрын

    We like to pin comments for clarifications or corrections. We have one part in the video where we had the cDNA spelled out and there is a misspelling - when the cDNA is spelled out, it should be spelled *complementary* DNA. Thanks again for the comment about this- we were able to correct!

  • @brianachavez6826

    @brianachavez6826

    3 жыл бұрын

    .t see

  • @songthanh896

    @songthanh896

    3 жыл бұрын

    Your videos are really helpful for a non-English speaking person like me to deal with studying my undergrad school. Thank you very much that I could graduate last Fall 2020.

  • @maryamramzan2407

    @maryamramzan2407

    2 жыл бұрын

    R

  • @rociobustamante5172
    @rociobustamante51723 жыл бұрын

    I'm 40, just started grad school, and taking a genetics class....you have no idea how useful your videos are. Thank you!

  • @longkevin11

    @longkevin11

    2 жыл бұрын

    Khan academy are great too

  • @brody2642

    @brody2642

    2 жыл бұрын

    Way to go! Hope you're doing well.

  • @BierBart12

    @BierBart12

    Жыл бұрын

    Hell yeah! I'm in a similar situation, but at an awesome evening school. This complicated stuff suddenly feels a thousand times easier than it did in morning school

  • @miau6451

    @miau6451

    Жыл бұрын

    Hope your grad career is going great! I love how Amoeba Sisters vids help everyone no matter their learning stage

  • @royalrod_
    @royalrod_3 жыл бұрын

    I already graduated from college , but I’m still here watching these videos

  • @221bbakerstreetfan4

    @221bbakerstreetfan4

    3 жыл бұрын

    In the same boat!

  • @beebowebo8906

    @beebowebo8906

    3 жыл бұрын

    bro same

  • @0dteESmini

    @0dteESmini

    3 жыл бұрын

    I'm skipping the college degree you paid for and just watching the free KZread videos. easy

  • @gaurabgain2782

    @gaurabgain2782

    3 жыл бұрын

    same here

  • @sarahabaza9684

    @sarahabaza9684

    3 жыл бұрын

    Same

  • @NavyBlueVinyl
    @NavyBlueVinyl3 жыл бұрын

    As a senior undergraduate in Biology, I treasure your videos. You girls are amazing! Saved me lots of time when I needed to review a subject for my Biochemistry or Molecular Biology lessons 😊

  • @michaelxchi

    @michaelxchi

    2 жыл бұрын

    same

  • @potat432
    @potat4323 жыл бұрын

    Thx so much!! I literally have a huge bio practise exam for feedback this afternoon

  • @mounirzouhari6453
    @mounirzouhari64533 жыл бұрын

    First of all, I just wanted to say that I really admire your video; the animation, the teaching style and how simple you make and present the concept is why you are my favorite channel.. You make the concepts that I really didn't understand easy and make me truly enjoy biology. I am sure that you are working super hard on your videos and in the same time you are a full time teacher, so it must be tiring . But i think a great way to improve your channel would maybe , in a another channel or even this one to make remake videos or make videos with all the details possible with the same animation and teaching style so that we can truly understand everything ( i am not saying that i am dissatisfied with your videos, i think they are near perfect) because when i try to watch KZread videos that explain more in detail , I have difficulty understand them . I just think that with your brilliant way of teaching and the amazing animation style that help us understand biology, you could make understand all your viewers even with the most difficult and detailed material. I know that it would take a large amount of time and I would understand if just didn't have the time but if its possible for you to make longer videos with more details, i think that it would really improve your channel and help the biology students to have a more grasp understand of biology.

  • @nidiaalvarado6923

    @nidiaalvarado6923

    3 жыл бұрын

    Actually, Pinky is a former biology teacher

  • @Smanalaifk

    @Smanalaifk

    3 ай бұрын

    they are not gonna reply to you be quiet

  • @thisgalisonfyr
    @thisgalisonfyr Жыл бұрын

    You guys are the only reason I'm passing biology class at age 36, hahaha. Thank you for what you do!

  • @emmanuelmakinde7694

    @emmanuelmakinde7694

    8 ай бұрын

    i believe in you

  • @nubbosaur
    @nubbosaur3 жыл бұрын

    These videos are helpful even after my physiology degree! They are working as great little refreshers while I study for the MCAT. Sometimes my mind feels too overwhelmed by the kaplan books and all of the heavy information I've been rehashing, so I come and watch these to give myself something to study thats quick, clear, and understandable without a head ache!

  • @camilacordero5718
    @camilacordero57183 жыл бұрын

    I had an exam with a question about PCR today and then this came out. I loved it anyway.

  • @jenniferdonatella172
    @jenniferdonatella1722 жыл бұрын

    Studying for a Biochemistry exam as part of my physician assistant program and came across this video! So thankful for your time to share your understanding. Will continue to use your page when studying in the future!! Thank you!

  • @DrReginaldFinleySr
    @DrReginaldFinleySr2 жыл бұрын

    Love it! This is my go-to source when outreaching and correcting the record about PCR for the general public. Thank you. There is so much disinformation out there we need more simple-to-understand videos like these. Keep up the amazing work.

  • @kelving3935
    @kelving39353 жыл бұрын

    Ever since my biology teacher introduced you, I have been LOVEING your biology videos it makes me laugh at the drawings and lets me focus on the facts!!

  • @brendalujanoalegria3327
    @brendalujanoalegria33272 жыл бұрын

    I´m doing my master degree and learning about the PCR and your videos are really useful! Thank you to combine the science with the creativity!!

  • @chicken8471
    @chicken84713 жыл бұрын

    I’ve already done PCR in a lab but the way you present it is so well that I watch it anyways 😂 great video!

  • @aishaibrahimusman3928

    @aishaibrahimusman3928

    2 жыл бұрын

    I’ll be doing my PCR in lab next month, if you don’t mind can we plz chat privately cuz I’ve some question to ask, kindly waiting 4 ur response, thanks

  • @shreyaaaaaa250
    @shreyaaaaaa2503 жыл бұрын

    Kay. To start off I don't usually comment on videos but I had to for this one. You guys are awesome like I'm a vivid yt watcher and I don't think I have come across anything that had me this engaged. Kudos to ya!! Keep being awesome you guys!!!!!

  • @salvadorvazquezreyes9672
    @salvadorvazquezreyes96723 жыл бұрын

    Your video is amazing! This is one of the best or the best that I had seen for a long time. I will share it with my undergrad students. Thank you for this great pedagogic tool, and keep up with these amazing animations.

  • @thatsnotme3235
    @thatsnotme32353 жыл бұрын

    Thank you for this! I looked for this over 3 weeks ago and couldn’t find nice videos like this. I’m so happy that you just posted this just in time

  • @noboolpit
    @noboolpit2 жыл бұрын

    Wow, this video is truly a godsend! Not only is the animation cute, but your upbeat, animated voice makes the content easily digestible! Thank you Amoeba Sisters!

  • @siddhantjadhav83
    @siddhantjadhav833 жыл бұрын

    wow you uploaded this video on perfect timing.... i just learned today about PCR but didn't had clear idea about it and automatically it popped in my recommendation as a gift ...thanks for such wonderful explanation ....keep up the good work!

  • @user-nz1zl7ux4o
    @user-nz1zl7ux4o3 жыл бұрын

    Amoeba Sisters I want to tell you. You are the lifesaver, every times I am going to have an exam your clips always work,it makes my life easier I doesn’t need to read a book and just look at your clips. It is amazing. Please continue this cute video forever.

  • @utkarshbhardwaj6837
    @utkarshbhardwaj68373 жыл бұрын

    Your videos really help me to understand the concepts! I thank you so much for these efforts 🤗 Love from India 😇🎉

  • @yasminedandan4900
    @yasminedandan49003 жыл бұрын

    U r making a great job, I used to watch ur videos when I was at college nd it helped me a lot! Thank u ✌🏼❤️

  • @Tanmay5616
    @Tanmay56162 ай бұрын

    I m studying Electrical Engineering and came across this concept while studying Biology for Engineers(academic sub).Enjoyed your video and also understood concepts in easy way.Thanks for it!

  • @elis2506
    @elis2506 Жыл бұрын

    I'm an undergrad student in college and you guys really help me visualize what I'm learning in class! Thank you so much :)

  • @lenalip815
    @lenalip8152 жыл бұрын

    the way i love your videos... idk how would i pass my biology class without them, thank u so much for doing this

  • @dominika4178
    @dominika41783 жыл бұрын

    I just decided to choose biochemistry as my thesis topic, and I am soo glad to find these videos! I love them! It helps me with my study a lot! Thank you soo much!!

  • @faiqarehman7679

    @faiqarehman7679

    2 жыл бұрын

    How u write ur thesis?

  • @areebullahmirza5579
    @areebullahmirza55793 жыл бұрын

    You guys come in clutch all of the time. I'm in biochem and molecular right now and I am so embarrassed I forgot everything from cell and micro bio.

  • @anvi5792
    @anvi57923 жыл бұрын

    You guys are amazing! You've helped me improve my Biology concepts. Take care guys and stay safe! Loads of love from India!

  • @panosr2135
    @panosr21353 жыл бұрын

    Idk how, but you always manage to upload the topics that I need a couple of weeks beforehand. Just thank you

  • @lizziebiology
    @lizziebiology3 жыл бұрын

    I love these so much! Im an A level biology teacher in England and my students and I love your videos/GIFs and everything! Thank you!!!

  • @132Conner
    @132Conner Жыл бұрын

    You guys are life savers! You make thing easy to understand and in a fun way!

  • @ilsem4674
    @ilsem46747 ай бұрын

    You do not know how much this videos mean to me. Without ur channel, I would never have been able to pass biology!

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    @abrahimdib10403 жыл бұрын

    i love watching you guys never stop posting

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    @mohamadtello62333 жыл бұрын

    I love this channel, what are you doing here is really great, it's a legacy... thank you soo much for helping others understanding science and especially in these times where we can't go to university lab and learn and the their online material are so boring!!

  • @deniseflaherty6723
    @deniseflaherty6723 Жыл бұрын

    Thank you so much for the high quality of your videos. They are a wonderful companion to my teaching.

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    @Anonymous-mf1cu3 жыл бұрын

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    @pmat27573 жыл бұрын

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  • @YohGS
    @YohGS3 жыл бұрын

    Great video!!!!! Thank you so much Amoeba sisters and welcome back!!

  • @groovy_9633
    @groovy_96333 жыл бұрын

    Can't thank y'all enough for saving me in biology. And in general.

  • @aislinn8522
    @aislinn85223 жыл бұрын

    Thank you for talking about COVID-19. I can really understand now. And I want to be a herpetologist, and these videos really help me understand the more complicated parts. You are my hero’s!

  • @haziqahzaini8326
    @haziqahzaini83263 жыл бұрын

    Thank youu~I really need this😭❤️

  • @mihaitmf
    @mihaitmf3 жыл бұрын

    Beautiful animation and explanations! Thank you for sharing!

  • @HotBeanJuiceCOFFEE
    @HotBeanJuiceCOFFEE3 жыл бұрын

    I love these channels because they make learning a lot better

  • @willie_wonka4061
    @willie_wonka40613 жыл бұрын

    I LOVE THISSSSS~~ THANK YOU SO MUCH!!

  • @sanikakadam4992
    @sanikakadam49923 жыл бұрын

    I really loved the video. I can't appreciate your creativity enough.

  • @emmiezzz1929
    @emmiezzz19293 жыл бұрын

    I had a science test today and your videos really helped me pass! Thank you for doing these videos!

  • @haixuan679
    @haixuan6793 жыл бұрын

    Another great video for my studies! Thank you so much for providing these awesome videoes during these tough times! ^_^

  • @vivelaresistance3239
    @vivelaresistance32392 жыл бұрын

    My son just got a job in a lab at UMich. Thanks for the video so I can ask him intelligent questions about what will form a large portion of his work.

  • @devinasyamnath3585
    @devinasyamnath358511 ай бұрын

    Creative, crisp and informational! Kudo sisters!

  • @user-pf3cu4lo7u
    @user-pf3cu4lo7u3 ай бұрын

    I love these videos. You guys make this legitimately entertaining

  • @kristinafernando1072
    @kristinafernando10723 жыл бұрын

    I LOOOOVE your videos and I've been watching them since high school! I'm about to finish college and I just love the evolution of this channel

  • @intermediatehandle21
    @intermediatehandle213 жыл бұрын

    Waited so much for the video. Love these biology videos.❤💝

  • @asfia_samar6014
    @asfia_samar60143 жыл бұрын

    Thank you so much..♡♡ I was searching for this topic Yesterday 😘 Thanks for uploading.

  • @finnelkington4521
    @finnelkington45212 жыл бұрын

    Thank you so much amoeba sisters! I find your animations so helpful

  • @xxbatmspiderxx2582
    @xxbatmspiderxx25827 ай бұрын

    I'm going insane

  • @sofieregner7293

    @sofieregner7293

    12 күн бұрын

    me too

  • @user-kc6gd5dw7k
    @user-kc6gd5dw7k2 ай бұрын

    Im a science olympiad student taking forensics and epidemology. These videos have helped me so so much!! Thank you for creating these easy videos and making my science olympiad life easier

  • @sofiaougo9765
    @sofiaougo9765 Жыл бұрын

    This is brilliant! Thank you for what you do, it means a lot

  • @ditebohomotlohi2790
    @ditebohomotlohi27903 жыл бұрын

    I'm writing my final life science paper 1 tomorrow at 9am and I am binging watching your videos. Thank you so much, I appreciate your content🥺🥺💕💕. Matric in 2020 is the pits and this channel is the reason why imma bag that distinction ❤️❤️❤️❤️

  • @ASH_LEY000
    @ASH_LEY0003 жыл бұрын

    how did you know i needed to learn this topic for my upcoming exam,, 🥺 you guys are a gem

  • @charliet3863
    @charliet38633 жыл бұрын

    I love these videos and I'm in high school. These never get old and are so cute.

  • @pranavmanoj9064
    @pranavmanoj90643 жыл бұрын

    Thanks!!! That was very informative and helped me understand the concepts you teach !! Keep making these awsome vids....

  • @spookywizard4980
    @spookywizard49802 жыл бұрын

    Adorable animations added to a great presentation!

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    @elistervowelles3 жыл бұрын

    I like your channel it helps me out is science class. I'm finally passing thanks to you and your sister! A huge thank you from a seventh grade girl!!

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    @elliscoulter94443 жыл бұрын

    Today was my first day of a genetics class, this will be helpful! :)

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    @beverlylapierre98944 ай бұрын

    Thank you! Your videos are very helpful and easy to understand.

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    @ludmilasorrentino12732 жыл бұрын

    You are AMAZING!!! Just found this video and it's wonderful. Thank you very much, really appreciate it!

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    @marcggs9345 Жыл бұрын

    This is the channel my teacher shows us!

  • @SuperNKM9
    @SuperNKM93 жыл бұрын

    Love all the funny references you made in the video. Copy machines are a blessing *when they work.*

  • @amshuamshu9504
    @amshuamshu95043 жыл бұрын

    Thank you so much today I found my study partner channel . Biotechnology was an inevitable burden for me . But because of you and your animation my dream is coming true .thank you once again

  • @anastasiarachel3704
    @anastasiarachel37043 жыл бұрын

    yayy new video from my favorite biology's lecturer :)

  • @mosaicrainbow6689
    @mosaicrainbow66892 жыл бұрын

    Very easy to understand! Thank you a million

  • @Just_kamara
    @Just_kamaraАй бұрын

    Thank you so much. Your channel has saved me a lot of time. Keep up the good work❤

  • @elle381
    @elle3812 жыл бұрын

    I love you so much. People like you are a gift to this world

  • @thunderbembo3406
    @thunderbembo34062 жыл бұрын

    injoyed watching this video. great presentation and explanation.

  • @LarissaOliveira-do7lo
    @LarissaOliveira-do7lo2 жыл бұрын

    I love the way u teach things, u videos helped me so much on graduacion

  • @dylanm1stry
    @dylanm1stry3 жыл бұрын

    Wooooow, thankyou this helped so much!! I'm doing an A level course and this explained it perfectly. :)

  • @cms3482
    @cms34823 жыл бұрын

    Thanks! I really need this. PCR is one of my weaknesses and after this video, TO THE KNOWLEDGE!! ^^

  • @joanacargaleiro
    @joanacargaleiroАй бұрын

    you're amazing, well done! thank you for clarifying so much this !

  • @imanyousaf3531
    @imanyousaf35313 жыл бұрын

    Hii, just wanted to say your videos help a lot!! Keep it up ❤

  • @aynazskt9350
    @aynazskt93503 жыл бұрын

    Thank you for your video. It was informative and to the point!

  • @syedaridazahra2507
    @syedaridazahra25073 жыл бұрын

    We would definitely STAY CURIOUS as we have a creative teacher like you!

  • @rockawo
    @rockawo7 ай бұрын

    Your videos are the funniest and greatest videos to learn!, also your recommended bibliography and videos are super interesting! Thank you so much Sisters! .... PD. I love Pinky and Petunia both, I simply can´t choose a favorite one XD

  • @DIDImoitout2810
    @DIDImoitout28103 жыл бұрын

    thank you !!! im from france this video is sooo clear even for other languages, understood everything thanks a lot

  • @blessyimmanuel6821
    @blessyimmanuel68213 жыл бұрын

    Wow!! Studying is super fun with amoeba sisters.

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    @andrearosado27013 жыл бұрын

    i love the amoeba sisters l love science so they help me a lot

  • @ellaliu7780
    @ellaliu77803 жыл бұрын

    I really love your videos they help a lot! Thanks!

  • @JiHyang
    @JiHyang3 жыл бұрын

    You guys are one of the youtubers who make me wanna study for fun, thank you! 💜💜💜

  • @ElevatorAdventure1816
    @ElevatorAdventure18163 жыл бұрын

    Holy Science this is my favorite channel thanks for helping me learn about some awesome science

  • @DOULOS1337
    @DOULOS13372 жыл бұрын

    amazingly well made video! Thank you ! very helpful!!

  • @nathanmigdal7960
    @nathanmigdal79603 жыл бұрын

    This is very helpful for a grad student being bombarded with new vocabulary terms. Thank you!

  • @AnythingDigital
    @AnythingDigital3 жыл бұрын

    Thank you!! I am in this area of biology, studying, right now ;)

  • @Mc06237
    @Mc062378 ай бұрын

    A very informative video. It will be an "indispensable💫" part of my research. Thank you so much❤

  • @LynTheWitch
    @LynTheWitch2 жыл бұрын

    Thanks i was a little rusty! So cool vids, must require so much work ! very well done and thank you again

  • @AmalRafi
    @AmalRafi2 ай бұрын

    Everything is really amazing and clear

  • @justnumber5197
    @justnumber51973 жыл бұрын

    Petunia art is getting better, I LOVE IT!! thank you amoeba sisters

  • @AmoebaSisters

    @AmoebaSisters

    3 жыл бұрын

    Made Petunia's day! Thank you! ≧◡≦

  • @mmd123king6

    @mmd123king6

    2 жыл бұрын

    @@AmoebaSisters does pcr copy dna at every replication time or onces and then it has a data of it?

  • @mmd123king6

    @mmd123king6

    2 жыл бұрын

    @@AmoebaSisters pls help me i have a exam... your the best lamoo

  • @andreasdixiyei3114
    @andreasdixiyei31143 жыл бұрын

    what a wonderful information. i love this video so much

  • @parllanpoppi3217
    @parllanpoppi32173 жыл бұрын

    Thank you Amoeba Sisters for this video. This was very helpful. 4:56 Btw, I remembered the "Tell me why" scene in this.

  • @randerson752
    @randerson7523 жыл бұрын

    Love this, very helpful in my new role sequencing DNA thank you.

  • @ASHISHVERMA-xh4dd
    @ASHISHVERMA-xh4dd3 жыл бұрын

    Beautiful illustration & explanation...

  • @ali42301
    @ali423012 жыл бұрын

    I can’t tell you how much that was helpful!