PCR (Polymerase Chain Reaction)
Join The Amoeba Sisters as they explain the biotechnology technique PCR. This video goes into the basics of how PCR works as well as two examples of its potential use.
Table of Contents:
00:00 Intro
1:34 How does PCR work?
4:31 Why use PCR?
5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19)
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Factual References (and excellent for learning more detail about this topic):
Clark, Mary Ann, et al. “17.1 Biotechnology - Biology 2e | OpenStax.” Biology 2e, OpenStax, 28 Mar. 2018, openstax.org/books/biology-2e/pages/17-1-biotechnology
www.fda.gov/consumers/consume...
www.fda.gov/media/136151/down...
*General length of a primer in base pairs from NIH www.genome.gov/genetics-gloss...
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FURTHER READING SUGGESTIONS:
On RT-PCR for SARS-CoV-2:
www.ncbi.nlm.nih.gov/pmc/arti...
jamanetwork.com/journals/jama...
On Fluorescent Probes in PCR:
www.ncbi.nlm.nih.gov/pmc/arti...
www.ncbi.nlm.nih.gov/pmc/arti...
What about limitations or disadvantages of PCR?
www.ncbi.nlm.nih.gov/pmc/arti...
What about more uses for PCR (as well as more info about Nobel Prize):
www.nobelprize.org/prizes/che...
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The Amoeba Sisters videos demystify science with humor and relevance. The videos center on Pinky's certification and experience in teaching biology at the high school level. Amoeba Sisters videos only cover concepts that Pinky is certified to teach, and they focus on her specialty: secondary life science. Learn more about our videos here: www.amoebasisters.com/our-videos
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TRANSLATIONS:
Thank you so much to our amazing translators!
Turkish subtitles by Kardelen Ünalleylioğlu
Hindi subtitles by Alisha Aggarwal
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Пікірлер: 516
We like to pin comments for clarifications or corrections. We have one part in the video where we had the cDNA spelled out and there is a misspelling - when the cDNA is spelled out, it should be spelled *complementary* DNA. Thanks again for the comment about this- we were able to correct!
@brianachavez6826
3 жыл бұрын
.t see
@songthanh896
3 жыл бұрын
Your videos are really helpful for a non-English speaking person like me to deal with studying my undergrad school. Thank you very much that I could graduate last Fall 2020.
@maryamramzan2407
2 жыл бұрын
R
I'm 40, just started grad school, and taking a genetics class....you have no idea how useful your videos are. Thank you!
@longkevin11
2 жыл бұрын
Khan academy are great too
@brody2642
2 жыл бұрын
Way to go! Hope you're doing well.
@BierBart12
Жыл бұрын
Hell yeah! I'm in a similar situation, but at an awesome evening school. This complicated stuff suddenly feels a thousand times easier than it did in morning school
@miau6451
Жыл бұрын
Hope your grad career is going great! I love how Amoeba Sisters vids help everyone no matter their learning stage
I already graduated from college , but I’m still here watching these videos
@221bbakerstreetfan4
3 жыл бұрын
In the same boat!
@beebowebo8906
3 жыл бұрын
bro same
@0dteESmini
3 жыл бұрын
I'm skipping the college degree you paid for and just watching the free KZread videos. easy
@gaurabgain2782
3 жыл бұрын
same here
@sarahabaza9684
3 жыл бұрын
Same
As a senior undergraduate in Biology, I treasure your videos. You girls are amazing! Saved me lots of time when I needed to review a subject for my Biochemistry or Molecular Biology lessons 😊
@michaelxchi
2 жыл бұрын
same
Thx so much!! I literally have a huge bio practise exam for feedback this afternoon
First of all, I just wanted to say that I really admire your video; the animation, the teaching style and how simple you make and present the concept is why you are my favorite channel.. You make the concepts that I really didn't understand easy and make me truly enjoy biology. I am sure that you are working super hard on your videos and in the same time you are a full time teacher, so it must be tiring . But i think a great way to improve your channel would maybe , in a another channel or even this one to make remake videos or make videos with all the details possible with the same animation and teaching style so that we can truly understand everything ( i am not saying that i am dissatisfied with your videos, i think they are near perfect) because when i try to watch KZread videos that explain more in detail , I have difficulty understand them . I just think that with your brilliant way of teaching and the amazing animation style that help us understand biology, you could make understand all your viewers even with the most difficult and detailed material. I know that it would take a large amount of time and I would understand if just didn't have the time but if its possible for you to make longer videos with more details, i think that it would really improve your channel and help the biology students to have a more grasp understand of biology.
@nidiaalvarado6923
3 жыл бұрын
Actually, Pinky is a former biology teacher
@Smanalaifk
3 ай бұрын
they are not gonna reply to you be quiet
You guys are the only reason I'm passing biology class at age 36, hahaha. Thank you for what you do!
@emmanuelmakinde7694
8 ай бұрын
i believe in you
These videos are helpful even after my physiology degree! They are working as great little refreshers while I study for the MCAT. Sometimes my mind feels too overwhelmed by the kaplan books and all of the heavy information I've been rehashing, so I come and watch these to give myself something to study thats quick, clear, and understandable without a head ache!
I had an exam with a question about PCR today and then this came out. I loved it anyway.
Studying for a Biochemistry exam as part of my physician assistant program and came across this video! So thankful for your time to share your understanding. Will continue to use your page when studying in the future!! Thank you!
Love it! This is my go-to source when outreaching and correcting the record about PCR for the general public. Thank you. There is so much disinformation out there we need more simple-to-understand videos like these. Keep up the amazing work.
Ever since my biology teacher introduced you, I have been LOVEING your biology videos it makes me laugh at the drawings and lets me focus on the facts!!
I´m doing my master degree and learning about the PCR and your videos are really useful! Thank you to combine the science with the creativity!!
I’ve already done PCR in a lab but the way you present it is so well that I watch it anyways 😂 great video!
@aishaibrahimusman3928
2 жыл бұрын
I’ll be doing my PCR in lab next month, if you don’t mind can we plz chat privately cuz I’ve some question to ask, kindly waiting 4 ur response, thanks
Kay. To start off I don't usually comment on videos but I had to for this one. You guys are awesome like I'm a vivid yt watcher and I don't think I have come across anything that had me this engaged. Kudos to ya!! Keep being awesome you guys!!!!!
Your video is amazing! This is one of the best or the best that I had seen for a long time. I will share it with my undergrad students. Thank you for this great pedagogic tool, and keep up with these amazing animations.
Thank you for this! I looked for this over 3 weeks ago and couldn’t find nice videos like this. I’m so happy that you just posted this just in time
Wow, this video is truly a godsend! Not only is the animation cute, but your upbeat, animated voice makes the content easily digestible! Thank you Amoeba Sisters!
wow you uploaded this video on perfect timing.... i just learned today about PCR but didn't had clear idea about it and automatically it popped in my recommendation as a gift ...thanks for such wonderful explanation ....keep up the good work!
Amoeba Sisters I want to tell you. You are the lifesaver, every times I am going to have an exam your clips always work,it makes my life easier I doesn’t need to read a book and just look at your clips. It is amazing. Please continue this cute video forever.
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I m studying Electrical Engineering and came across this concept while studying Biology for Engineers(academic sub).Enjoyed your video and also understood concepts in easy way.Thanks for it!
I'm an undergrad student in college and you guys really help me visualize what I'm learning in class! Thank you so much :)
the way i love your videos... idk how would i pass my biology class without them, thank u so much for doing this
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@faiqarehman7679
2 жыл бұрын
How u write ur thesis?
You guys come in clutch all of the time. I'm in biochem and molecular right now and I am so embarrassed I forgot everything from cell and micro bio.
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Idk how, but you always manage to upload the topics that I need a couple of weeks beforehand. Just thank you
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BEST CHANNEL EVER Short Simple Interesting U've been helping me for 3 years straight thank you so much
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I love these channels because they make learning a lot better
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Thank you so much..♡♡ I was searching for this topic Yesterday 😘 Thanks for uploading.
Thank you so much amoeba sisters! I find your animations so helpful
I'm going insane
@sofieregner7293
12 күн бұрын
me too
Im a science olympiad student taking forensics and epidemology. These videos have helped me so so much!! Thank you for creating these easy videos and making my science olympiad life easier
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Thanks!!! That was very informative and helped me understand the concepts you teach !! Keep making these awsome vids....
Adorable animations added to a great presentation!
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yayy new video from my favorite biology's lecturer :)
Very easy to understand! Thank you a million
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I love you so much. People like you are a gift to this world
injoyed watching this video. great presentation and explanation.
I love the way u teach things, u videos helped me so much on graduacion
Wooooow, thankyou this helped so much!! I'm doing an A level course and this explained it perfectly. :)
Thanks! I really need this. PCR is one of my weaknesses and after this video, TO THE KNOWLEDGE!! ^^
you're amazing, well done! thank you for clarifying so much this !
Hii, just wanted to say your videos help a lot!! Keep it up ❤
Thank you for your video. It was informative and to the point!
We would definitely STAY CURIOUS as we have a creative teacher like you!
Your videos are the funniest and greatest videos to learn!, also your recommended bibliography and videos are super interesting! Thank you so much Sisters! .... PD. I love Pinky and Petunia both, I simply can´t choose a favorite one XD
thank you !!! im from france this video is sooo clear even for other languages, understood everything thanks a lot
Wow!! Studying is super fun with amoeba sisters.
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amazingly well made video! Thank you ! very helpful!!
This is very helpful for a grad student being bombarded with new vocabulary terms. Thank you!
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A very informative video. It will be an "indispensable💫" part of my research. Thank you so much❤
Thanks i was a little rusty! So cool vids, must require so much work ! very well done and thank you again
Everything is really amazing and clear
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@AmoebaSisters
3 жыл бұрын
Made Petunia's day! Thank you! ≧◡≦
@mmd123king6
2 жыл бұрын
@@AmoebaSisters does pcr copy dna at every replication time or onces and then it has a data of it?
@mmd123king6
2 жыл бұрын
@@AmoebaSisters pls help me i have a exam... your the best lamoo
what a wonderful information. i love this video so much
Thank you Amoeba Sisters for this video. This was very helpful. 4:56 Btw, I remembered the "Tell me why" scene in this.
Love this, very helpful in my new role sequencing DNA thank you.
Beautiful illustration & explanation...
I can’t tell you how much that was helpful!