Pasteurization vs Sterilization Experiment (Part 1)

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In the previous video I talked about why my manure substrate seems to keep contaminating with trichoderma. Well, someone had posted a comment on that video stating that sterilization works much better than pasteurization for nutritious substrates, which is the complete opposite of what the mainstream understanding is. But who knows? I've never tried so I thought this would make an interesting experiment for you all to see what truly is. I doubt this will work but I hope I'm wrong!
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Пікірлер: 80

  • @Mycophilia
    @Mycophilia Жыл бұрын

    A *LIKE* and a *COMMENT* puts wind in my sails! 💨⛵

  • @Eliqueme

    @Eliqueme

    Жыл бұрын

    😶‍🌫️

  • @goombaspore7386

    @goombaspore7386

    Жыл бұрын

    Taco Bell will do that too

  • @jaksmith6465
    @jaksmith64655 ай бұрын

    sterilization is 250F. thats why you need the pressure. its not pressure that sterilizes, the pressure allows steam to go past 212F

  • @LillianGardner0214
    @LillianGardner0214 Жыл бұрын

    👌My dude. Appreciate the time you take to convey thousands of hours of experience / knowledge in a cogent video format.

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    🙏

  • @NetsarimTheWatchman
    @NetsarimTheWatchman5 ай бұрын

    Always enjoying your content!

  • @goombaspore7386
    @goombaspore7386 Жыл бұрын

    Can’t wait to see the results

  • @markjauregui8138
    @markjauregui8138 Жыл бұрын

    Interesting video. I stumbled on your channel while searching, I enjoy watching your videos where you breake everything down in simple easy to understand terms. I also like how you dont use reddit for go to answers.... I will definetly be following this series. I am new to this and was thinking about composting my chicken, goat, and duck poo for using in some trials. I believe it takes a while to compost it down to reduce the nitrogen, and also balance the PH which is some what critical on many levels for using to add to substrates. I can not wait to see how your journey goes. Thanks for your great videos....

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Thank you for your comment, glad to have you here!

  • @uopuol
    @uopuol Жыл бұрын

    i had questions about pasteurization v sterilization but for straw, and it is great seeing your thoughts on a similar matter- & like urself i may have to just fuck around and find out through observation and experiments to get the answer i want

  • @rkaith
    @rkaith Жыл бұрын

    Great video

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Thanks!

  • @treehouseconstituents6402
    @treehouseconstituents6402 Жыл бұрын

    Hey what's up bro! I've been so busy with everything here, my apologies for not filling you in, however...I just noticed lol so that Colloidal Silver Tek I was experimenting with..... amazing!!! I covered my plates in CS and actually forgot about them during my move into the new place, I pulled them out 2dys ago expecting catastrophe, it was quite the opposite actually... The 2 plates I treated and didn't touch again were completely clean! I actually lost a whole spore syringe of my top producer, but when I pulled out the CS test plates and saw how thick the ryzomorphic growth had become and how healthy it looked I about dropped the plate lol. I cut 8 specimens from each plate transferring to agar. They exploded almost overnight. So I can say with confidence that my CS Tek will work. CS will destroy any bacteria with single celled walls, the mycelium isn't bothered because it has thick double celled walls. I'm going to up the ante and put CS into the water I use for grain spawn and everything else and see how that works. I'm willing to bet that if one were to add CS to their Agar recipe it would manage contamination phenomenally. Ok I'm getting back to work it's been rough lol.

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Hey THC! I’ve just been thinking about messaging you! Great to hear that the CS is working great. I’m curious to see how it would fair against dirty swabs and outdoor spores. Great to see you back here!

  • @treehouseconstituents6402

    @treehouseconstituents6402

    Жыл бұрын

    @@Mycophilia same I think it'd be a good idea to isolate individual contaminates onto agar and test each one with the CS. It's relatively inexpensive and I'm using a 10ppm solution, they make it up to 500ppm. Imagine soaking your grane spawn in a CS solution and eliminating contam on almost every front. If this continues to prove fruitful the implications would be incredible, open air work stations, no LFHs or SABs. It's good to be back too! Glad to see things are still rolling for you. I hope KZread fixes your channel. With several states and municipalities legalization it shouldn't be a problem anymore. I'll keep my fingers crossed. NAMASTE 🙏 I'll hit you up again shortly. Reach out anytime you'd like.

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    @@treehouseconstituents6402 thanks THC! 🍄 ❤️

  • @Leancr88

    @Leancr88

    Жыл бұрын

    ​​@@treehouseconstituents6402I would love to hear an update on this 😮

  • @aswfishing4319
    @aswfishing4319 Жыл бұрын

    Love it

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Thanks, glad to hear that!

  • @jennifererin6808
    @jennifererin6808 Жыл бұрын

    As always great content my friend . Tried to email the link in the description and it said invalid ??you will laugh my tubs are listening to your channel .

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    mycophilia.official@gmail.com 😊

  • @jennifererin6808

    @jennifererin6808

    Жыл бұрын

    @@Mycophilia nope tried and it said it either does not exist or cannot except

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    @@jennifererin6808 That's very strange, here's my backup email: mycophilesage@gmail.com

  • @JonathanYankovich
    @JonathanYankovich7 ай бұрын

    If you can pasteurize nutritious substrate, could you theoretically pasteurize grain for spawn?

  • @Mycophilia

    @Mycophilia

    7 ай бұрын

    No as grain is far too nutritious. There’s a reason they’re sterilized.

  • @georgesimmons9815
    @georgesimmons981510 ай бұрын

    I don't understand why people believe the 'bad' organisms are killed but 'good' are preserved in pasteurization. Is there science to support that?

  • @Mycophilia

    @Mycophilia

    10 ай бұрын

    Yes, of course.

  • @5.dot.connector

    @5.dot.connector

    3 ай бұрын

    nothing that would be known on Wikipedia. As far as it is explained there pasteurization simply does reduce the bacterial load where sterilization brings it to (near) zero. As far as I can tell heat and pressure don't distinguish between "good" and "bad" bacteria. They kill microorganisms according to their relative resistance to heat / pressure. And what's beneficial anyway? perhaps a "low" bacterial or yeats load is. or perhaps some specific strands are beneficial. I haven't seen any hard science on it so far.

  • @gfifplayer4691

    @gfifplayer4691

    Ай бұрын

    ​@@5.dot.connectorso-called beneficial bacterial in this case is termophilic bacterial (im not sure of this is correct english termin, but they are love heat and wher it Is too hot for others they are thrive). And this becterias can live in substrat and give it some contaminat resistant. But what is more important - they will not mess with our mushrooms. Somehow they are not competing for the substrat

  • @klaymistic4810
    @klaymistic4810 Жыл бұрын

    How do you pasteurize your substrate?

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    12 hours on the keep warm setting in an instantpot

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Substrate brought to field capacity, and contained in jars

  • @TheFarmacySeedsNetwork
    @TheFarmacySeedsNetwork Жыл бұрын

    Thanks for sharing... Keep up the great work!

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Thank you! Will do!

  • @gangsterbuilder
    @gangsterbuilder Жыл бұрын

    Can you grow the good bacteria and then sterilize and then add the growing good bacteria?

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Probably but that’s kinda beyond me to isolate the good bacteria.

  • @gangsterbuilder

    @gangsterbuilder

    Жыл бұрын

    @@Mycophilia with cannabis they make rooting hormones and good micrbos and have plenty from my growing days. have you tried any?

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    @@gangsterbuilder no but mushrooms are completely different organisms than plants

  • @gangsterbuilder

    @gangsterbuilder

    Жыл бұрын

    @@Mycophilia humans were a fungus a few 100 million years ago. Few people know that we are more closely related to fungi than to any other kingdom 650 million years ago

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    @@gangsterbuilder yes I’m aware

  • @danwilkinson2797
    @danwilkinson2797 Жыл бұрын

    I found a solution to your problem. Hardwood sawdust make a tea use it for soaking grains and for hydration of substrate. You will never see green again. I got that tip from home mycology. It’s the tannins.

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Got the video? I’d like to see it

  • @danwilkinson2797

    @danwilkinson2797

    Жыл бұрын

    @@Mycophilia I went looking for the video on the home mycology channel couldn’t find it. Perhaps the forums have some reference to it somewhere. I would say it doesn’t hurt.Im not scientific enough to do a extensive experiment but perhaps some one somewhere has . I think he just credited the hippie for the tec .

  • @DC_DC_DC_DC

    @DC_DC_DC_DC

    Жыл бұрын

    ​@@Mycophilia will you feature this in an experiment please?

  • @captainfalcon8302
    @captainfalcon83025 ай бұрын

    Putting your substrate in a bucket with boiling water and letting it sit over night is that pasteurizing the substrate? I’m new to growing for give my ignorance

  • @Mycophilia

    @Mycophilia

    4 ай бұрын

    That’s not pasteurization, that’s called bucket TEK and what it does is essentially cook coir and hydrate it. Pasteurization (or sterilization) is not necessary with coir and vermiculite.

  • @droidnick

    @droidnick

    3 ай бұрын

    Why is it not nessasay, ecause no poo? ​@Mycophilia

  • @thomasmorrison8933
    @thomasmorrison8933 Жыл бұрын

    💎🤔🖤

  • @Enochulate88
    @Enochulate88 Жыл бұрын

    Lebanon 🌲🌲🌲

  • @Enochulate88

    @Enochulate88

    Жыл бұрын

    🌲🌲🌲🌲🌲

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Lebanon

  • @DangerRussDayZ6533
    @DangerRussDayZ6533 Жыл бұрын

    You talk about pasteurization time and temperatures, but you're not talking about how you're pasteurizing. Are you using an over? Are you boiling? or???

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    In jars inside a slow cooker

  • @DangerRussDayZ6533

    @DangerRussDayZ6533

    Жыл бұрын

    @@Mycophilia Correct me if I'm wrong, but I haven't seen you make a video about that. That would definitely be interesting as I haven't seen that tek b4

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    @@DangerRussDayZ6533 I use to have one but I’ll repost/remake it!

  • @DangerRussDayZ6533

    @DangerRussDayZ6533

    Жыл бұрын

    @@Mycophilia That would be great. I'm also interested in your coir only tek, because I believe I heard you mention that you do not need to pasteurize coir if you're only using coir. I'll definitely be staying tuned for this video, if you decide to make it. Thanks

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    @@DangerRussDayZ6533 👍

  • @spacecityryder
    @spacecityryder Жыл бұрын

    Comment

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    🙏!

  • @bradzimmer239
    @bradzimmer239 Жыл бұрын

    I'm sorry but I've done both and have zero contam with pasteurization. I won't sterilze anymore.

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    Why are you sorry lol

  • @grantwilliams7453
    @grantwilliams74536 ай бұрын

    SUPER PASTEURIZATION

  • @AviatorDave
    @AviatorDave Жыл бұрын

    Saga: I never had good luck with the Moo Cow Poo that has wood in the mix, this is like opening the door for the Mean Green.

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    That is interesting that you mention the wood, as I had my suspicions about that as well. Thanks for the comment Dave!

  • @treehouseconstituents6402

    @treehouseconstituents6402

    Жыл бұрын

    What if you did small isolated sterilizations instead of pasteurizations, with an overnight presoak in a Colloidal Silver solution? Are you scavenging for feces locally? If you're scavenging for local crap, and said local crap has wood, the wood has been through the digestive tract and there's minimal you can do to get the contaminants out of that wood especially if it's bovine. The digestive system basically softens the wood and it will absorb everything inside the cows body it comes into contact with during the digestive process. In that case I would still recommend small isolated batches however during the pasteurization/sterilization (whatever you choose) increase the length of your process by atleast 3x if you can get the wood poop to maintain a temp around 170°F for long enough it should have an effect but still add Colloidal Silver with your field capacity process as that will either eliminate or retard the process so much you'll get a fruiting and a flush or 2 I would hope. I've been experimenting with Colloidal Silver a bit with great success. And the mycelium doesn't mind it much at all. The tomentose growth may retreat a bit, however it will come right back with a fervor. It may turn your mycelium blueish, I'm not sure if my blues were from the silver or the "Silo Sin" oxidizing within the mycelium, I know for a fact the blue was NOT contam. I've discovered that the mycelium will bruise blue when you touch it or cut specimens just like it's fruit, especially the higher percentage genetics. Apparently the mycelium has quite a bit of "blue love" lol. I plan to experiment With this by growing and harvesting a large sample of healthy mycelium and doing an extraction and distillation of the "contents" to see. I got the idea from some lab analysies of percentages of Cordycepin contained within the Cordyceps fruiting bodies as opposed to the mycelium. Cordycep Mycelium contains a noticably higher percentage of cordycepin than it's fruits.

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    @@treehouseconstituents6402 I’am really coming to suspect the poo now. Next time I’ll just buy a bag of pure cow poop and see how that fares, nonetheless I’m happy to see both of you talk about the wood as I had my suspicions. What tripped me up though was how some people don’t have a problem even with the wood. But all I know is that the casing layer is pasteurized fine with the same method every single time, whereas this compost contaminated 100% of the time. I’m going to try pure cow manure

  • @treehouseconstituents6402

    @treehouseconstituents6402

    Жыл бұрын

    @@Mycophilia lmk how that works bro, I'm still doing synthetic dung with pharmaceutical grade chemical nutrients, I make a nutrient broth out of Pure nitrogen crystals, humic acid extract from coal and compost, phosphorus, kelp extract, sodium, Calcium, magnesium, Sulphur, cellulose, flavonoids and polyphenols. It's a little harder to locate pure polyphenols and flavonoids at a reasonable price though.

  • @rearm2046
    @rearm2046 Жыл бұрын

    remember pasteurization and sterilization is useless if you have contaminated spawn even if you dont see it

  • @davids11131113

    @davids11131113

    Жыл бұрын

    Not necessarily, I’ve seen a bit of contam get beaten by mycelium before, both in tubs and on agar plates. That’s what I’m always telling these growers who show a small spot of contam on their second flush, and calling it ruined as you’ve got another ounce of dried weight that you can harvest tomorrow, it’s not a death sentence don’t throw it out.

  • @thomasmorrison8933
    @thomasmorrison8933 Жыл бұрын

    FAFO series

  • @urbanforager90
    @urbanforager90 Жыл бұрын

    Horse manure works the best i get dried poo balls from a friend with a horse

  • @magicalbird5500
    @magicalbird5500 Жыл бұрын

    🍄🍄👽⛴

  • @Mycophilia

    @Mycophilia

    Жыл бұрын

    🐻

  • @jeremyjetson1965
    @jeremyjetson1965 Жыл бұрын

    50 shades of poo