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Microscope tutorial - How to stain with H&E

A taster of the type of microscope tutorials that we run at QUT in Brisbane.
In this video I discuss how to optimise the level of hematoxylin staining through use of a "differentiation" step (brief treatment in acidified alcohol).

Пікірлер: 9

  • @eclipsegrenda7925
    @eclipsegrenda79258 ай бұрын

    This is useful to me, i have an examination tomorrow at Mbarara university in uganda❤, thenks

  • @damienharkin

    @damienharkin

    8 ай бұрын

    You are most welcome. Good luck with the exam!

  • @qiranchen5097
    @qiranchen5097 Жыл бұрын

    Great video! Will fixatives affect the staining of nuclei by Hematoxylin (in terms of the bouin's and paraformaldehyde)?

  • @damienharkin

    @damienharkin

    Жыл бұрын

    Hi Qiran, yes, fixation technique can impact significantly on staining outcomes. The picric acid in Bouin’s fixation can improve retention of trichrome stains making them seem more vibrant. I haven’t examined specifically affects on nuclear staining, but pH can certainly affect binding and retention of hematoxylin.

  • @goharrehman7710
    @goharrehman77104 жыл бұрын

    please if you can some videos on antigens on the cancers immunohistochemistry and stains

  • @goharrehman7710
    @goharrehman77104 жыл бұрын

    please if you can make a videos on fixatives and the special stains

  • @pratishthamadaan1038
    @pratishthamadaan10383 жыл бұрын

    Make more videos pls

  • @damienharkin

    @damienharkin

    3 жыл бұрын

    More are planned. Let me know if you have any requests.

  • @eclipsegrenda7925

    @eclipsegrenda7925

    8 ай бұрын

    Diff quick pliz