LGC's KASP Genotyping Chemistry Explained: The KASP reaction
Ғылым және технология
KASP is a PCR-based genotyping chemistry suitable for genotyping SNPs and InDels. KASP has been used to genotype millions of samples worldwide. If you have you ever wondered “How does KASP work?”, this series of three videos explains the KASP reagents, the genotyping reaction and how the data is analysed. This is the second video and shows what happens when you run a KASP genotyping reaction.
The example shown in this animation is a SNP genotyping KASP reaction. It gives a step-by-step explanation of the early rounds of the PCR and then goes on to explain how the alleles present in the DNA sample will result in a fluorescent single from the completed KASP reaction.
This video demonstrates how the KASP genotyping reaction would report DNA samples that are homozygous or heterozygous for the target SNP.
After the reaction is completed, and the fluorescence has been measured, it is necessary to analyse the date. Please watch the next video to see how this is done.
Learn more about KASP genotyping at www.lgcgroup.com/kasp/
For more information about LGC’s products and services visit www.lgcgroup.com
Пікірлер: 7
beautiful!!! :D ... thanks a lot for the animation... great explanation :D
Well described the process
Do anybody know why KASP markers are longer than usual markers ? Thanks
I wonder if the probe-oligonucleotide will be denatured (separated from quencher) during the first round of PCR and emit signal ? or the probe has to bind on the template DNA to emit signal ?
@nattaphatpanyasang1297
3 жыл бұрын
From my perspective, the probe, can emit signal in the first place, but it is inhibited by quencher. Therefore, in the first round the X/Y complement haven't been created yet. The probe will eventually end up with the quencher, but in the third round, the complement is created and the probe bind to it, in the other words, the probe is permanently separated from sequence which allow it to emit the signal.
@funkymonkey787
2 жыл бұрын
I think the signal is only read at the end of the PCR-reaction. At this stage, when the temperature is lowered, the unbound reporters will bind again with the quenchers. Therefore no signal is emitted when the results are interpretted.
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