🍄 Is this a rare mushroom? DNA Barcoding Part 1: DNA Extractions + PCR
Ғылым және технология
In this weekly vlog, Dr Jenny Shelton takes you behind the scenes of the DNA analysis lab on her kitchen table and shows that real world biosciences can be accessible to everyone.
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Bento is creating tools, reagents, and guides to unlock the power of bioscience. Our mission is to make DNA analysis accessible to anyone, anywhere.
Bento Lab is an all-in-one DNA workstation, combining centrifuge, PCR, and gel visualisation.
Пікірлер: 11
Hi everyone, it's Jenny here! As one eagle-eyed viewer of this vlog spotted (thanks David!), I did the dipstick extraction slightly wrong. Thankfully it still worked for the PCR and (spoiler alert!) sequencing. Moral of the story: double-check when using a new protocol, especially when you're filming a vlog! For a demonstration of the dipstick DNA extraction method, please view our video here: kzread.info/dash/bejne/g3ajo6qBZNjWebA.html
Im a myco nerd! Im into wild preservation, cultivation, breeding gourmets. Nothing better to me than working with agar in front of a fan filter unit. The way mycelium grows is fascinating to me. I have been wanting to learn dna barcoding. I think i have some studying to do! Ur video was fascinating and informative
Bento Lab is too cool. Looking forward to Part II.
@BentoLab
2 жыл бұрын
Thanks! We're also curious what the results will be... :)
a tip for getting clean uncontaminated tissue is to rip the stem into two parts and cut out a sample from the inside. don't use a blade to cut open the tissue, since the blade is "draging" contamination from the outer surface along with the cut.
@fatimamoazam4490
2 ай бұрын
hy..i find your idea quite cool...but what if we cut the stem with a blade that is sterlized on the spot (with spirit) ? or how can we cut the stem without blade i couldnt understand this..thank you
@aidennymes6335
2 ай бұрын
@@fatimamoazam4490 just use your sanitized hands and pull it apart without touching the inside
Can't wait to get mine!!!!
what is the extraction buffer made of? is it possible to use a dish soap & NaCl solution like the one used to extract dna from strawberries?
WOOOOOHOOOOOOOO
how high did it get you?