IR Spectroscopy and Mass Spectrometry: Crash Course Organic Chemistry #5

It’s time for molecular analysis! On this episode of Crash Course Organic Chemistry, we’re learning about mass spectrometry and infrared spectroscopy through the lens of a forensic investigation. Put on your lab coats, and let’s solve this mystery!
Episode Sources:
Silverstein, R. M., Webster, F. X., Kiemle, D. J., & Bryce, D. L (2007). Spectrometric identification of organic compounds, 8th ed., Hoboken, NJ: John Wiley & Sons, Inc.
Spectra for pseudoephedrine: BioRad/Sadtler Data Collection, Bio-Rad Laboratories, Philadelphia, PA (US), Spectra ID: BR125952
Spectra for methamphetamine: Kunalan, V., Daeid, N. N, Kerr, W. J., Buchanan, H. A. S., McPherson, A. R., “Characterization of Route Specific Impurities Found in Methamphetamine Synthesized by the Leuckart and Reductive Amination Methods,” Anal Chem, 2009, 81, 7342-7348.
Other spectra reproduced with permission from: SDBSWeb : sdbs.db.aist.go.jp/ (National Institute of Advanced Industrial Science and Technology, 6/9/2020.
Series Sources:
Brown, W. H., Iverson, B. L., Ansyln, E. V., Foote, C., Organic Chemistry; 8th ed.; Cengage Learning, Boston, 2018.
Bruice, P. Y., Organic Chemistry, 7th ed.; Pearson Education, Inc., United States, 2014.
Clayden, J., Greeves, N., Warren., S., Organic Chemistry, 2nd ed.; Oxford University Press, New York, 2012.
Jones Jr., M.; Fleming, S. A., Organic Chemistry, 5th ed.; W. W. Norton & Company, New York, 2014.
Klein., D., Organic Chemistry; 1st ed.; John Wiley & Sons, United States, 2012.
Louden M., Organic Chemistry; 5th ed.; Roberts and Company Publishers, Colorado, 2009.
McMurry, J., Organic Chemistry, 9th ed.; Cengage Learning, Boston, 2016.
Smith, J. G., Organic chemistry; 6th ed.; McGraw-Hill Education, New York, 2020.
Wade., L. G., Organic Chemistry; 8th ed.; Pearson Education, Inc., United States, 2013.
***
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Пікірлер: 148

  • @animeandstuff5377
    @animeandstuff53774 жыл бұрын

    My condolences to those who didn’t get to watch this series while in organic chemistry 😔

  • @Laaaa

    @Laaaa

    4 жыл бұрын

    Anime and Stuff this series is about 12 years too late for me 😩

  • @dogzrzosmz

    @dogzrzosmz

    4 жыл бұрын

    I feel this deeply

  • @saumyamathur4862

    @saumyamathur4862

    4 жыл бұрын

    I took it this same time last year, & while I’m happy that today’s ochem students have this at their fingertips, I’m also very jealous that I didn’t 😩😂

  • @kevinconrad6156

    @kevinconrad6156

    4 жыл бұрын

    Condolences accepted. My lecture teacher was good when he stayed on topic which he did not do often. The lab teacher was great and often gave short lectures on what the lecture teacher missed.

  • @birzhanabdikhan8175

    @birzhanabdikhan8175

    4 жыл бұрын

    Totally agree brother

  • @MrShipp100
    @MrShipp1004 жыл бұрын

    My PhD is in advanced IR spectroscopy using femtosecond lasers, and this is a really nice concise explanation of the basics!

  • @definty

    @definty

    4 жыл бұрын

    Isn't that just a laser that turns on and off very fast? Or is it something different?

  • @alarcon99

    @alarcon99

    4 жыл бұрын

    @Shippers I stan

  • @extremdeath1234

    @extremdeath1234

    4 жыл бұрын

    @@definty spectroscopy on the femtosecond time scale is a bit more than "very fast". It is ludicrously fast... you can actually monitor reaction on that time scale. for perspective, it takes about 300fs for molecular iodine to vibrate once...

  • @user-ik6wc1rn4j

    @user-ik6wc1rn4j

    4 жыл бұрын

    WAIt , u are saying this is the BASIC?

  • @grad3us

    @grad3us

    4 жыл бұрын

    Please, I want CrashCourse Neurology! It would be really cool to understand the brain, just as it was cool to understand computer science.

  • @joymae
    @joymae4 жыл бұрын

    I’m digging the purple accents in your backdrop and how it matches your sweater

  • @user-xx7pg3vw9k

    @user-xx7pg3vw9k

    4 жыл бұрын

    Joy Mae that was no accident...

  • @glennng7612
    @glennng76124 жыл бұрын

    As a chem major, I really love how concise the explanations are. Great job crash course!

  • @xxstargirlxx
    @xxstargirlxx4 жыл бұрын

    I saw Abby(from NCIS) So cute

  • @anne12876
    @anne128764 жыл бұрын

    Side note, during my first internship as chemistry student, I worked as a lab technician at the anti-doping centre for sports. I spent a summer analysing urine samples by IR spectrometry and sometimes by MS to check for illicite drugs. I was fun to see what happens in the lab during big big sports events and the science behind it.

  • @ariame2722
    @ariame27224 жыл бұрын

    The molecules dancing made my day 😭

  • @EverythingScience
    @EverythingScience4 жыл бұрын

    I gotta say these videos seem much more advanced than the normal topics you guys cover

  • @MysteryMan1910
    @MysteryMan19104 жыл бұрын

    Just finished my PhD in structural mass spec. This is a pretty good intro! Although, at least in my field, no one really uses the magnetic sector MS instruments shown here anymore - we more often uses ToF, ion trap or orbitrap mass spectrometers.

  • @TheTravelerww
    @TheTravelerww4 жыл бұрын

    the T.V scientist in the graphic; is that meant to be Abby Sciuto from NCIS, great reference.

  • @mawatake
    @mawatake4 жыл бұрын

    I'm a Japanese, but I understand this content because of English subtitle ! I want to watch this series about 1H-NMR and 13C-NMR

  • @TheTwick
    @TheTwick4 жыл бұрын

    I’ve always enjoyed that scene in a movie when a grizzled old detective runs his fingers through a black substance at a scene and says to the young detective “Send this back to the lab and have it analyzed.”!

  • @christianandrews7764
    @christianandrews77644 жыл бұрын

    Was not expecting this but I’m liking the progression of this series! Looking forward to episode 6

  • @zagisch4789
    @zagisch47894 жыл бұрын

    I’m writing a home exam about the use of analytical chemistry in archaeology atm, so the timing of this is perfect 😍

  • @RedwoodGeorge
    @RedwoodGeorge4 жыл бұрын

    A long time ago I worked for a company that made Fourier Transform Infrared Spectrometers. All new employees went through the two week customer training course to learn how the machines worked. In our class one of the customers was an evidence tech from the Royal Canadian Mounted Police - I was sitting next to an actual Mountie! Cool! One of the attachments for the spectrometer was a microscope - the Mountie had brought along a sample paint chip with three layers of different color paint. We focused the microscope on each layer and ran the results through the database to identify the brand, color and year of each layer! For even deeper analysis, we worked with a GC / Mass Spec to split up the sample by boiling point and molecular weight. The boiled off results were deposited on a cold plate in a labyrinth pattern and then the spectrometer sampled along the path to further analyze the components. Those were fun days working with some pretty cool machinery :-)

  • @CrossingDS
    @CrossingDS4 жыл бұрын

    As I biochemist, I found it very complete but also clear. Also, loved the effort put into the actual video!

  • @radunicoara8057
    @radunicoara80574 жыл бұрын

    I have finally learned what mass spectroscopy is. As a physics major, I never really understood it. Incredibly good course!

  • @user-pr1st5rh9r
    @user-pr1st5rh9r Жыл бұрын

    I just love this channel .. their way of teaching along with case studies, various examples is just so awesome & practical.❤️👍🏻Keep it up guys !

  • @BenTajer89
    @BenTajer894 жыл бұрын

    As somebody who's already been through highschool and undergrad O-Chem, I really wish your explanation of the mass spec had gone just a TINY bit more in depth, you describe all the parts and how the ion goes through, but none of how the magnet actually separates the masses as they go around the curve. This concept is actually super intuitive.

  • @emilyensing2184
    @emilyensing21844 жыл бұрын

    I love the major mass spec and abby reference in cartoon form!

  • @farhanmendel
    @farhanmendel4 жыл бұрын

    Crash Course about different analytical chemistry techniques and methods would be cool!

  • @foodaddict17
    @foodaddict174 жыл бұрын

    Love love love this! :D Explained so clearly

  • @sheepyboi4219
    @sheepyboi42194 жыл бұрын

    Omg omg thankk youuuu. I have my Chemistry exam next week and this is something that is the hardest for me in unit one thanks so much!!

  • @knate44
    @knate444 жыл бұрын

    Omg. Can't wait for the inevitable Crash Course Analytical chemistry!

  • @michelleelizabeth9956
    @michelleelizabeth9956 Жыл бұрын

    Wonderful videos and beautiful explanations , best of all. Thank you for sharing.

  • @timr8431
    @timr84314 жыл бұрын

    I have a chem degree and this even helped me understand it a little better. Great video

  • @kai_taylor6550
    @kai_taylor65504 жыл бұрын

    Love this i really needed to know this and its a great example thanks Crash Course Crew!!

  • @TriplaHHH
    @TriplaHHH4 жыл бұрын

    Awesome :D Analytics gets often disregarded in chemistry-pop due to its complex backdrop. So thanks for the effort to lay this out somehow! Disregarding the instrumentation and datahandling though, the basic concepts of sample prep, separation and measurement in analysis are quite straightforward. Sounds like we are heading towards chromatography ;)

  • @enobnala90
    @enobnala904 жыл бұрын

    This is the only part of Organic Chemistry that I able to absorb!

  • @bakryabdelmonem2066
    @bakryabdelmonem20664 жыл бұрын

    Amazing you making me love chemistry even more

  • @Darfanatior
    @Darfanatior4 жыл бұрын

    Crash Course is the coolest!

  • @UgoTemple
    @UgoTemple4 жыл бұрын

    Organic Chemistry is one of my favorite

  • @Karajorma

    @Karajorma

    4 жыл бұрын

    @vitali Mizrachi You're telling him what is his favourite?

  • @RJ-nr8lh
    @RJ-nr8lh4 жыл бұрын

    PLEASE BRING BACK CRASH COURSE LITERATURE. PLEASE WE LOVED THAT.

  • @johnstevens1020
    @johnstevens10204 жыл бұрын

    YAYYY Another video from crash course!!!!!

  • @kevinfleming9918
    @kevinfleming99184 жыл бұрын

    Great explanation!

  • @thebloxxer22
    @thebloxxer224 жыл бұрын

    You referenced Abby from NCIS in the beginning.

  • @VintageBlacklist

    @VintageBlacklist

    4 жыл бұрын

    Abby is the best.

  • @atomicxfox
    @atomicxfox4 жыл бұрын

    please make an in-depth video on IR and proton NMR graph interpretation!

  • @dangriff12

    @dangriff12

    4 жыл бұрын

    If you have any specific questions just message me.

  • @Ogiwon
    @Ogiwon4 жыл бұрын

    looks great! :)

  • @sofbsilva
    @sofbsilva4 жыл бұрын

    You're about 5 days late. I had an exam about this on friday...

  • @bidishadey3815

    @bidishadey3815

    4 жыл бұрын

    🤣🤣🤣

  • @Veronica-wz8ow
    @Veronica-wz8ow4 жыл бұрын

    I love you guys

  • @saurra3953
    @saurra39534 жыл бұрын

    Yay I was just reading this from Clayden!

  • @LucyRockprincess
    @LucyRockprincess Жыл бұрын

    excellent video

  • @pyritium
    @pyritium3 ай бұрын

    What does DFTBA in the dialogue bubble over Deboki in the intro animation stand for?

  • @grahamrankin4725
    @grahamrankin47254 жыл бұрын

    Ephedrine and pseudoephedrine will have almost identical MS and IR because they are diastereoisomers. Both can be used to make meth.

  • @PavanKumar-xv1hg

    @PavanKumar-xv1hg

    4 жыл бұрын

    thank you ?

  • @geovannamartins2243
    @geovannamartins22434 жыл бұрын

    Great video! Greetings from Brazil!

  • @pamelaevangelista434
    @pamelaevangelista4346 ай бұрын

    Im an ib chem student and lemme tell you. Youre doing gods work, youre a miracle sent down from heaven, A NATIONAL TREASON!!! So thank you🥹, i might actually pass this class💀😵

  • @enitmarin6805
    @enitmarin68054 жыл бұрын

    Genial job.

  • @honeybeewarrior5731
    @honeybeewarrior57314 жыл бұрын

    Incredible science

  • @omsingharjit
    @omsingharjit4 жыл бұрын

    Awesome Brilliant great

  • @finexjoshy9651
    @finexjoshy96514 жыл бұрын

    The new app does not load and when the first screen had loaded it showed that there was some error. Then I tried again and it still did not load even after 20 minutes

  • @xpalin1980
    @xpalin19804 жыл бұрын

    ABBY! I love abby from NISC she’s so cute :3

  • @sceptre1067
    @sceptre10674 жыл бұрын

    the ongoing use of purple triggers my youth watching Burke’s Connections in a good way. i feel old. 🤪

  • @nicholasticali4623
    @nicholasticali46234 жыл бұрын

    This stuff is really awesome and was really fun, but it also brings back really really bad memories 😂

  • @KillerTacos54
    @KillerTacos544 жыл бұрын

    Our SAC is next week haha. Thank you for going over all this stuff. I think what would've been really helpful would be going through the different reaction pathways as well as H-NMR and C-NMR (because that's in our SAC). Thankfully, IR Spectroscopy is in it and mass spec was going to be in it, but it got taken out as well as chirality and geometric isomers. This stuff was taken out of our study design (syllabus) because of COVID-19 and everything. Btw if anyone else does VCE in Victoria, Australia: hi :). After that, we're moving onto chromatography like HPLC, paper chromatography and stuff

  • @dangriff12

    @dangriff12

    4 жыл бұрын

    I need one on 2D NMR. It just looks like chicken pox and I have no idea what I am looking at. Any C13 NMR or DEPT 135 is fine though just message me any specifics.

  • @ZalyQQ
    @ZalyQQ4 жыл бұрын

    I hope you teach HPLC before I need to use it 6 weeks :/

  • @Synthetica9
    @Synthetica94 жыл бұрын

    Is that tucos compound from bb?

  • @techgirl4977
    @techgirl49774 жыл бұрын

    am i the only one who thinks crash course should be subscribed to by everyone in the world

  • @zarahasan331
    @zarahasan3314 жыл бұрын

    lol I have a lab final for this tomorrow... wish me luck.

  • @dutchik5107
    @dutchik51074 жыл бұрын

    I had an exam on this last week. I barely passed. Could've used it earlier 😓

  • @michaelmayhem350
    @michaelmayhem3504 жыл бұрын

    You should make an hours log video of you just reading from textsbooks for us to listen to (no video necessary but it's never a bad thing)

  • @dangriff12
    @dangriff124 жыл бұрын

    30 minutes is a long time to get a spectra! Even after running a blank to clean out the mass spec ours takes 3.5 minutes per run. So 7 minutes for the first spectra and 3.5 minutes for each subsequent spectra. It's not like the machine was super expensive either I think $4K.

  • @papachoudhary5482
    @papachoudhary54824 жыл бұрын

    Thanks

  • @jeffreypomeroy6173
    @jeffreypomeroy61734 жыл бұрын

    So how do you tell the difference between chiral molecules?

  • @TriplaHHH

    @TriplaHHH

    4 жыл бұрын

    Other techniques, like chiral column chromatography (i.e. pre-separation of chiral components, then detection with mass spec), or Raman spectroscopy (infrared-zone laser excitation. Raman optical activity experimentation is quite new, so I might make a mistake here). According to Wikipedia, there's also a possibility to apply kinetic resolution (i.e. react the isolated rasemic (both chiralities) mixture with a catalyst differentiated by the reaction rate.

  • @kacibjordan
    @kacibjordan4 жыл бұрын

    That’s Abby from NCIS!!

  • @jv4779
    @jv47794 жыл бұрын

    How do these processes deal with impure samples?

  • @ImmieHands

    @ImmieHands

    4 жыл бұрын

    I'm currently working on my PhD in mass spec so probably alright to answer this. There are a couple of things we do to deal with impurities, firstly couple MS with some form of chromatography like GC or LC which separates out different compounds in a mixture based on their relative affinity for either the stationary or mobile phase, meaning different compounds enter the mass spec at different times. Along with that, if whatever you're studying is in a particularly dirty matrix, for instance I work with wastewater samples, you can perform various extraction techniques to remove the chemicals of interest from the sample matrix, leaving behind the impurities. Hope this helps!

  • @Greenmachine305
    @Greenmachine3054 жыл бұрын

    It was a meth lab explosion. Got it.

  • @sofia.eris.bauhaus

    @sofia.eris.bauhaus

    4 жыл бұрын

    no it was a pseudoephedrine lab smh

  • @danieljohnson3024

    @danieljohnson3024

    4 жыл бұрын

    Someone had sinus congestion and couldn't smell the gas from when the pilot light went out.

  • @maksimghyvoronsky5641

    @maksimghyvoronsky5641

    4 жыл бұрын

    sofia eris bauhaus You know pseudoephedrine is like one of the main ingredients in meth right? And since it was a LAB then yeah... what Bryan C. said

  • @amjadilham4174
    @amjadilham41744 жыл бұрын

    now i realize purple is actuallly dope

  • @itstotallyezra
    @itstotallyezra4 жыл бұрын

    We use spectrometry in archaeology !!!!!!!

  • @henryelicker2403
    @henryelicker24034 жыл бұрын

    I'm getting mixed signals from this video.

  • @zarinawillows2347
    @zarinawillows23474 жыл бұрын

    13:16 VERY SMALL Mystery ?!?!!? You call that small....

  • @antonyandrewson5803
    @antonyandrewson58034 жыл бұрын

    where was this when I was failing my a levels :'(

  • @mohamedgamaleldeen4315
    @mohamedgamaleldeen43154 жыл бұрын

    How do I review the approach of data structures and algorithms before I study software engineering course Thanks

  • @andeexists8716
    @andeexists87164 жыл бұрын

    I wish I had these videos during A Level Chemistry lmaoooo

  • @Ft888x

    @Ft888x

    4 жыл бұрын

    Same!

  • @samiebelal8700
    @samiebelal87004 жыл бұрын

    Dude I just finished ochem dangit

  • @hogo21
    @hogo214 жыл бұрын

    Ngl, I watched this just cause I saw Abby. Video is great though!!!

  • @DennisKanji254
    @DennisKanji2544 жыл бұрын

    Spot on!!!

  • @kasualstudio7141
    @kasualstudio71414 жыл бұрын

    Those 16 dislikes are Hollywood producers

  • @Felixkeeg
    @Felixkeeg4 жыл бұрын

    NMR best spectroscopy

  • @Adenybaloi
    @Adenybaloi4 жыл бұрын

    Yo, it's Abby

  • @katiefinch9414
    @katiefinch94144 жыл бұрын

    Boy I hope Starburns made it out ok

  • @taefasiri6999
    @taefasiri69994 жыл бұрын

    please we need to translation to Arbec 🙂💔

  • @websurfer5772
    @websurfer5772 Жыл бұрын

    Has any scientist ever even seen a proton or neutron? If not, how do they even know what element they're working with? Also, how do they know they're not just detecting and measuring the electrons they bombarded the substance with instead of the "ions" from the sample?

  • @emilybentley7502

    @emilybentley7502

    Жыл бұрын

    I'm not sure how to answer your first question, but as for the second one: electrons are negatively charged, whereas the ions produced in a mass spec are positively charged. They will move in different directions in response to the magnet - only the positive ions will end up on the detector. There might be other reasons too but I know that one!

  • @ArsalanKhan-ki3rx
    @ArsalanKhan-ki3rx4 жыл бұрын

    Where is didi sabrina cruz

  • @grad3us
    @grad3us4 жыл бұрын

    Please, I want CrashCourse Neurology! It would be really cool to understand the brain, just as it was cool to understand computer science.

  • @Inadharion
    @Inadharion4 жыл бұрын

    I spent the first minute coming to terms with your name. The rest of the video was obviously way above my head.

  • @gibranhenriquedesouza2843
    @gibranhenriquedesouza28434 жыл бұрын

    If I had known it would be so hard to find a job in physics, I would have done chemistry ...

  • @uneditedvideos6492
    @uneditedvideos64924 жыл бұрын

    what is the "white powder"?

  • @RamdomView
    @RamdomView4 жыл бұрын

    Annoyed that I forgot the meaning of the wave numbers.

  • @joshr.5199
    @joshr.51994 жыл бұрын

    Where was this in September !? Jkjkjk i did fine

  • @mist2666
    @mist26664 жыл бұрын

    on tiktok his name is hankgreen1

  • @danteller8282
    @danteller82824 жыл бұрын

    I'm not entirely sure if I agree with the way you pronounce "cation", but that's a pretty common area of contention. If we lived anywhere near each other, I would totally use that as a premise to ask you out.

  • @kevinconrad6156
    @kevinconrad61564 жыл бұрын

    Something makes me think that Dr. Chakravarti likes purple.

  • @memlafeder
    @memlafeder4 жыл бұрын

    Why nobody tasted the white powder? It is easier and instant result. (go hollywood science!)

  • @ChadWilson
    @ChadWilson4 жыл бұрын

    Purple!

  • @lokilaufeyson935
    @lokilaufeyson9354 жыл бұрын

    I tried to be 1st and I failed. It think I’m about 3rd though 😊

  • @Vertigotrueshot
    @Vertigotrueshot4 жыл бұрын

    what about an Anti-Mass Spectrometer.

  • @Karajorma

    @Karajorma

    4 жыл бұрын

    Well first you need to convert your lab to run on dark energy.

  • @zohaibhassan3786
    @zohaibhassan37864 жыл бұрын

    Please make videos on history of Pakistan and also on criminology

  • @joaovitormatos8147
    @joaovitormatos81474 жыл бұрын

    Let's put a burning building on the thumbnail. What can go wrong?

  • @josenellandrewtumulak2400
    @josenellandrewtumulak24004 жыл бұрын

    Dope

  • @TheBobbytables
    @TheBobbytables4 жыл бұрын

    Anyone else here to rep Major Mass spec?