How to turn any light microscope into a dark field scope

For less than $1 US, convert nearly any light microscope into a dark field microscope. Commercial conversion kits can cost upwards of $100. This cheap, easy, fully reversible technique allows even the most basic 'scopes to be upgraded.
(c) 2014 Ambithusia

Пікірлер: 107

  • @alylyshua74937
    @alylyshua749374 жыл бұрын

    Dark field always strikes me because of the beautiful colors. Thanks for sharing!

  • @luapynneb3069
    @luapynneb30696 жыл бұрын

    After watching this and doing a little more searching for ideas, I 3d printed a range of sized discs to fit in to my filter holder, which is at the same place you placed your blackout sheet you made, works good to about 0.65 Objectives (4x 10x 40x). One thing I noticed on my microscope was I had to raise the condenser to sit a few MM under the slide to get the dark-field to work. Big thanks for sharing this info as you saved me £80-£100. ; )

  • @usernamemykel
    @usernamemykel9 жыл бұрын

    You're the Alton Brown of microscopy. Well done, thank you. More, please.

  • @pmac5621
    @pmac56218 жыл бұрын

    Thank you very much Dan! I was considering a dark field, but the price was too high, so having this ability to make a lightfield into a darkfield is much appreciated.

  • @ewu2030
    @ewu2030 Жыл бұрын

    just ordered a microscope today and I was looking for ways to view darkfield on it ( quite cheap, no commercial adapter available) and with your amazing solution, it's brilliant! thanks man!

  • @ChrisFuchser
    @ChrisFuchser5 жыл бұрын

    Thanks! Suggest simplifying by cutting the opaque spots out of black construction paper, instead of using a Sharpie marker and stacking. You can just put a dab of adhesive on the construction paper dot to attach it to the clear plastic.

  • @wispafish
    @wispafish4 жыл бұрын

    Gave a thumbs up just for the 'something somthing' bit of the vid lol. Thank you for the advice, I'm going to try dark field now!

  • @pattirayne1243
    @pattirayne12433 жыл бұрын

    Thank you! If you will pardon the pun, I found your presentation "illuminating". I just got the wrong type of microscope for what I'd like to do and you gave me so much inspiration. I'll follow and subscribe so my thanks becomes a tangible thing. Carry on!

  • @shaileshkris
    @shaileshkris3 жыл бұрын

    I have this strong urge to come down and help you maintain precision! Thoroughly enjoyed the content, great work, and I got to learn something!

  • @danieltadeubraido2967
    @danieltadeubraido29676 жыл бұрын

    Thank you very much for the video tutorial. I was just needing that for my microscope. You're great!

  • @StargazerFS128
    @StargazerFS1289 жыл бұрын

    Well I'll tell ya that was just about the most useful vid I've seen on youtube in a long while, super job on that, I'll be scoping out plastic sheets pronto! Thx

  • @Fifita2012
    @Fifita20128 жыл бұрын

    Very good idea! Thank you so much for sharing this with us ! I am going to make my light compound microscope dark field now :D

  • @Robodie
    @Robodie2 жыл бұрын

    "Something something something dark field..." Just waking up, thank you for the first laugh of the day! Better than a cup of coffee.

  • @masellipictures
    @masellipictures6 жыл бұрын

    Excellent tutorial and explanation, Bravo!

  • @majornabeel598
    @majornabeel5987 жыл бұрын

    Excellent work Dan!

  • @lornelemaire5661
    @lornelemaire56615 жыл бұрын

    very nice, as an amateur I appreciate your video, keep up the good work!

  • @hsuanhuang404
    @hsuanhuang4047 жыл бұрын

    Thanks, easy and simple. Keep the good work

  • @theblogfather
    @theblogfather6 жыл бұрын

    Hi, thanks for the video. I found it very informative and helpful to get more use from my microscope.

  • @Thinkchronous
    @Thinkchronous7 жыл бұрын

    Thanks for this useful Video. It confirmed by suspicion that darkfield is a very simple modification.

  • @Seyhan60
    @Seyhan608 жыл бұрын

    We need more people like you on KZread, thanks! :)

  • @WillyDrengen

    @WillyDrengen

    Жыл бұрын

    Check out microbehunter

  • @Seyhan60

    @Seyhan60

    Жыл бұрын

    @@WillyDrengen thanks, I just discovered his channel :)

  • @WillyDrengen

    @WillyDrengen

    Жыл бұрын

    @@Seyhan60 No problem, Microbehunter is just such a great guy.

  • @donaldeisenbarth5255
    @donaldeisenbarth52552 жыл бұрын

    Thank you; this I will try. I accidentally partially blocked light from the condenser with a small ruler and ended up with a partial dark field. It helped with the contrast.

  • @Jennycantho
    @Jennycantho9 жыл бұрын

    Thank you very much! I'm looking for buying new one darkfield condenser but now I am trying to create it.

  • @paulwisniewski6551
    @paulwisniewski65518 жыл бұрын

    thanks, i enjoyed your video, it was informative and concise

  • @rockstomper8390
    @rockstomper83904 жыл бұрын

    Thanks Dan!!

  • @angelcaudillo402
    @angelcaudillo4026 жыл бұрын

    Very very useful! Well done!! Thank you very much :)

  • @martinramirez9671
    @martinramirez96716 жыл бұрын

    Excelent and funny video. I just buy a microscope and I am eager to convert it in a dark field microscope. I will try these great idea. Thank you.

  • @BrittanyASMR
    @BrittanyASMR9 жыл бұрын

    Fantastic, thank you!

  • @Mrtfrimet
    @Mrtfrimet8 жыл бұрын

    Awesome. Best tutorial ever!

  • @riccardostefani1966
    @riccardostefani19663 жыл бұрын

    great tutorial, thank you so much!!

  • @indranilpahari334
    @indranilpahari3348 жыл бұрын

    great work .

  • @jgrafsheppard
    @jgrafsheppard9 жыл бұрын

    I really enjoyed this.

  • @HighFidelityFox
    @HighFidelityFox7 жыл бұрын

    Thanks! It worked very well!

  • @usernamemykel
    @usernamemykel6 жыл бұрын

    Terrific, thanks!

  • @murielfoucart860
    @murielfoucart8607 жыл бұрын

    Excellent ! Thank you so much :)

  • @ianhodgson2149
    @ianhodgson21496 жыл бұрын

    Much appreciated top job. Thankyou.

  • @MICROWORLDofficial
    @MICROWORLDofficial6 жыл бұрын

    Oh, thank you very much for that cool idea =)

  • @borissman
    @borissman6 жыл бұрын

    You are a good speaker

  • @jc10907Sealy
    @jc10907Sealy5 жыл бұрын

    Genius! I’ll use it thx

  • @spiritualwarriorhealer6154
    @spiritualwarriorhealer61545 жыл бұрын

    I love your midnight movie voice! LoL!!

  • @michaelrapheal4772
    @michaelrapheal47726 жыл бұрын

    Thanks for the tip!! When will you be producing another video?

  • @Broxine
    @Broxine9 жыл бұрын

    thats just awesome. thank you for this tutorial :-) are you going to make some more videos ? you are good at it

  • @rafaassuncao5433
    @rafaassuncao54333 жыл бұрын

    Thanks sir!

  • @danielzavala3739
    @danielzavala37392 жыл бұрын

    Very clever... Now please show us how to update it into a phase contrast xD

  • @joewebster903
    @joewebster9035 жыл бұрын

    Excellent

  • @lainthdavidmartinez3974
    @lainthdavidmartinez39744 жыл бұрын

    Nice video bro.

  • @senanlane6882
    @senanlane68824 жыл бұрын

    Thanks

  • @healthesoils
    @healthesoils4 ай бұрын

    soooooo good thank you 🙏

  • @Samsasi77
    @Samsasi779 жыл бұрын

    thank u ...so help ful msg ...keep it up ..

  • @marcmarc172
    @marcmarc172 Жыл бұрын

    Niceeee! This is a killer microscope 'hack'. Thanks!

  • @danielribastandeitnik9550
    @danielribastandeitnik95506 жыл бұрын

    Thanks!! I was researching here, it's not that expensive slide sheets to print on a laser printer (fortunatly I already have such a printer). I'll try printing some!

  • @robertpendergast2620
    @robertpendergast26208 жыл бұрын

    Works well after some time spent with fitting. I made one a couple years ago. How about polarizing for the DIY person?

  • @ellencalabrese5308
    @ellencalabrese53084 жыл бұрын

    Thankyou

  • @hyperhektor7733
    @hyperhektor77337 жыл бұрын

    here this one can be done also easyand cheap, just 2 polarising filters , 1 for the lightsource and 1 for the Eyepiece (CPL Filters can be found from 3$ each in the analog cameras section on ebay for example on ebay in your country or on ebay china)

  • @zazugee

    @zazugee

    7 жыл бұрын

    that's not darkfield, it's phase contrast you're appearing to be doing here.

  • @michaelb.1860

    @michaelb.1860

    4 жыл бұрын

    That’s neither darkfield nor phase contrast but...wait for it...polarized light aka polarization contrast

  • @h7oslo
    @h7oslo9 жыл бұрын

    Great vid! Good presentation, simple, inexpensive, effective! Tnx!!! rc

  • @mammanemma5352
    @mammanemma5352 Жыл бұрын

    Sir you are a wizard

  • @K3Flyguy
    @K3Flyguy2 жыл бұрын

    I make em from plastic bottle caps. Very rigid, no cost and easy to work with

  • @punishalltrolls
    @punishalltrolls8 жыл бұрын

    The field can be darkened further by enlarging the shaded diameter. Can we use a similar technique for solar filters on telescopes?

  • @funny11744
    @funny117443 жыл бұрын

    Nice video ! Nice person.

  • @PEARLING-EXCELLENCE
    @PEARLING-EXCELLENCE4 жыл бұрын

    Nice information, do you have any trick for inverted microscope to turn them in phase contrast such easier way?

  • @lotharmayring6063
    @lotharmayring60637 ай бұрын

    olique illumination is better than dark field. Simply use LEDs putting them a bit aside on filterholder

  • @JohnHoranzy

    @JohnHoranzy

    6 ай бұрын

    I have done that at low magnification. Now I have the cash for the real thing. Hope I am not disappointed with it.

  • @PrasetyoMuhammadDwiBiology
    @PrasetyoMuhammadDwiBiology8 жыл бұрын

    omg we need this :D

  • @magdastoksik1384
    @magdastoksik13849 жыл бұрын

    Great, for the beginner to understand the case - super!!! What about polarization and DIC? I just bought a microscope and all that extra stuff is so expensive and i have no idea how to apply it :-) i am gratefull for help.

  • @eqlzr2
    @eqlzr25 жыл бұрын

    How do I know when I have the right diameter for the light block? Thx.

  • @migmagingenieria
    @migmagingenieria6 жыл бұрын

    Thank you so much for your vid. Please give me some advice, I am growing some lactobacillus cultures (now trying to replicate some L. Reuteri). I would like to be able to see the action and make videoclips and pictures. Would an Omax 40-2500x work, is that enough amplification??? Good luck!!

  • @christinahinton4538
    @christinahinton45383 жыл бұрын

    My thumbs up came at the something something something part😂😂😂

  • @MasudO313
    @MasudO3134 жыл бұрын

    Nice, i need help or direction. I want to see water clusters. Is thier a way.

  • @ravenm.4396
    @ravenm.43964 жыл бұрын

    I love you.

  • @JamieDelour
    @JamieDelour8 жыл бұрын

    Hi Dan! What kind of microscope would you recommend a beginner that wants to view red blood cells under darkfield? Thanks for this DIY!

  • @haveaniceday7950

    @haveaniceday7950

    Жыл бұрын

    This is my question as well. Did you ever find the answer?

  • @haveaniceday7950
    @haveaniceday7950 Жыл бұрын

    What would be a good low cost scope for viewing live blood?

  • @ZanteF12R
    @ZanteF12R8 жыл бұрын

    How do you film the microscope? do you have a usb lens or you put the camera in front of the eyepiece?

  • @Sara_London
    @Sara_London4 жыл бұрын

    This might be a silly question but could you do something like this with a flashlight and jewlers loup. By covering the light from the flashlight with the black plastic and looking thru the loup? I know nothing about microscopes! I still have a lot of learning to do. I've been looking at gems as a hobby. I stare at them for hours with different magnification loups and a flashlight. I keep hearing about "dark field illumination" when I watch gem identification videos on KZread. Until I can get my own microscope I thought maybe this might be a very rough diy way! Thanks for the video... now when I do get a microscope I'll know how make my own dark field :) Plus it was funny! Nicely done!

  • @thedealer799
    @thedealer7992 жыл бұрын

    I wonder if you could just paint the coin you use black and glue it to the transparent material.

  • @disasterexperiments493
    @disasterexperiments4934 жыл бұрын

    Asewome!!!

  • @Metalsenpai
    @Metalsenpai4 жыл бұрын

    Thanks. That´s only works in 5x and 10x. Someone know how to make that in 40x or 100x? I discovery a very easy way to do that , with 5x and 10x, with a specific kind of filter suporter: open the diaphragm and move the filter suporter to the right until you get the image. Will work too.

  • @gabrielyosei7565

    @gabrielyosei7565

    3 жыл бұрын

    I have the same problem with 40x

  • @philiptaylor2567
    @philiptaylor25677 жыл бұрын

    I tried this method to look at the spirochetes in my blood, and it worked well at 10x. But when I try it at 100x with immersion oil it doesn't work. The image shows up faded and looking like a poor lit version of a light field microscope. Do have any ideas why this would be, and how I can make this work for high power with immersion oil?

  • @wesremy

    @wesremy

    7 жыл бұрын

    Phillycheesecreampie Cake u need a smaller cirkel for bigger magnifications

  • @wesremy

    @wesremy

    7 жыл бұрын

    Phillycheesecreampie Cake no just kidding u cant get darkfield with high aperture objectives

  • @philipwilkin2667

    @philipwilkin2667

    7 жыл бұрын

    Uhm... Why are there spirochetes in your blood?

  • @vortexor1

    @vortexor1

    7 жыл бұрын

    Did you get it managed to see good pictures of the blood in 100x?

  • @petetheweet
    @petetheweet6 жыл бұрын

    Great idea, thanks for sharing. Wouldn’t it be quicker and easier to glue something opaque onto a single plastic sheet instead of colouring several sheets? Or am I missing something?

  • @Nihaikitty

    @Nihaikitty

    5 жыл бұрын

    I was wondering this as well.

  • @michaelb.1860

    @michaelb.1860

    4 жыл бұрын

    Yes that would work just as well - opaque disc should be as thin as possible

  • @benibuntu2052
    @benibuntu2052Ай бұрын

    ❤❤❤ ⚘️🌷⚘️. .Thank You Verry Much..🙏🙏😊😊😊👍👍👍

  • @rockstomper8390
    @rockstomper83904 жыл бұрын

    Meet Dan, He is a BAMF!!

  • @DianneElizabeth64
    @DianneElizabeth642 жыл бұрын

    So it’s not true or true because of strength that only Arizona & Nevada allow dark field microscopes

  • @alexpopblock6435
    @alexpopblock64354 жыл бұрын

    can't you just colour a paper circle in black and cover it in plastic circles

  • @ellencalabrese5308
    @ellencalabrese53084 жыл бұрын

    I gotta look at some mites from the dog

  • @jackemled
    @jackemled8 жыл бұрын

    THANK YOU!!! I FINALLY FOUND SOMETHING ON MAKING A DARK FIELD FILTER!!! **Explodes.**

  • @miguelelento5637
    @miguelelento56375 жыл бұрын

    Brightfield

  • @ahmedraza1587
    @ahmedraza15872 жыл бұрын

    That guy used is Brain 🧠= 110% 😅

  • @MrBrianlowdown
    @MrBrianlowdown Жыл бұрын

    Somthing Somthing Somthing...not cheap...Ha!!! You can say that about so many things today...

  • @christiandietachmair334
    @christiandietachmair3347 жыл бұрын

    I think 7 don´t like this Idea becauce they are selling Darkfield Microscopes .

  • @abergethirty
    @abergethirty6 жыл бұрын

    What is with your odd enunciations ?

  • @picometer472
    @picometer4723 ай бұрын

    stop waiving your hands in the face of the viewer--it;s offensive

  • @rezzer7918
    @rezzer7918 Жыл бұрын

    Moronic bordering on bonafide lunacy

  • @ravanabrahmarakshas4263
    @ravanabrahmarakshas42634 ай бұрын

    brilliant. you saved me a few hundred dollars. thanks.

  • @spondoolie6450
    @spondoolie6450 Жыл бұрын

    I am here from the future to tell you to buy Dogecoin.... you'll tank me later

  • @angelcaudillo402
    @angelcaudillo4026 жыл бұрын

    Very very useful! Well done!! Thank you very much :)