How to turn any light microscope into a dark field scope
For less than $1 US, convert nearly any light microscope into a dark field microscope. Commercial conversion kits can cost upwards of $100. This cheap, easy, fully reversible technique allows even the most basic 'scopes to be upgraded.
(c) 2014 Ambithusia
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Dark field always strikes me because of the beautiful colors. Thanks for sharing!
After watching this and doing a little more searching for ideas, I 3d printed a range of sized discs to fit in to my filter holder, which is at the same place you placed your blackout sheet you made, works good to about 0.65 Objectives (4x 10x 40x). One thing I noticed on my microscope was I had to raise the condenser to sit a few MM under the slide to get the dark-field to work. Big thanks for sharing this info as you saved me £80-£100. ; )
You're the Alton Brown of microscopy. Well done, thank you. More, please.
Thank you very much Dan! I was considering a dark field, but the price was too high, so having this ability to make a lightfield into a darkfield is much appreciated.
just ordered a microscope today and I was looking for ways to view darkfield on it ( quite cheap, no commercial adapter available) and with your amazing solution, it's brilliant! thanks man!
Thanks! Suggest simplifying by cutting the opaque spots out of black construction paper, instead of using a Sharpie marker and stacking. You can just put a dab of adhesive on the construction paper dot to attach it to the clear plastic.
Gave a thumbs up just for the 'something somthing' bit of the vid lol. Thank you for the advice, I'm going to try dark field now!
Thank you! If you will pardon the pun, I found your presentation "illuminating". I just got the wrong type of microscope for what I'd like to do and you gave me so much inspiration. I'll follow and subscribe so my thanks becomes a tangible thing. Carry on!
I have this strong urge to come down and help you maintain precision! Thoroughly enjoyed the content, great work, and I got to learn something!
Thank you very much for the video tutorial. I was just needing that for my microscope. You're great!
Well I'll tell ya that was just about the most useful vid I've seen on youtube in a long while, super job on that, I'll be scoping out plastic sheets pronto! Thx
Very good idea! Thank you so much for sharing this with us ! I am going to make my light compound microscope dark field now :D
"Something something something dark field..." Just waking up, thank you for the first laugh of the day! Better than a cup of coffee.
Excellent tutorial and explanation, Bravo!
Excellent work Dan!
very nice, as an amateur I appreciate your video, keep up the good work!
Thanks, easy and simple. Keep the good work
Hi, thanks for the video. I found it very informative and helpful to get more use from my microscope.
Thanks for this useful Video. It confirmed by suspicion that darkfield is a very simple modification.
We need more people like you on KZread, thanks! :)
@WillyDrengen
Жыл бұрын
Check out microbehunter
@Seyhan60
Жыл бұрын
@@WillyDrengen thanks, I just discovered his channel :)
@WillyDrengen
Жыл бұрын
@@Seyhan60 No problem, Microbehunter is just such a great guy.
Thank you; this I will try. I accidentally partially blocked light from the condenser with a small ruler and ended up with a partial dark field. It helped with the contrast.
Thank you very much! I'm looking for buying new one darkfield condenser but now I am trying to create it.
thanks, i enjoyed your video, it was informative and concise
Thanks Dan!!
Very very useful! Well done!! Thank you very much :)
Excelent and funny video. I just buy a microscope and I am eager to convert it in a dark field microscope. I will try these great idea. Thank you.
Fantastic, thank you!
Awesome. Best tutorial ever!
great tutorial, thank you so much!!
great work .
I really enjoyed this.
Thanks! It worked very well!
Terrific, thanks!
Excellent ! Thank you so much :)
Much appreciated top job. Thankyou.
Oh, thank you very much for that cool idea =)
You are a good speaker
Genius! I’ll use it thx
I love your midnight movie voice! LoL!!
Thanks for the tip!! When will you be producing another video?
thats just awesome. thank you for this tutorial :-) are you going to make some more videos ? you are good at it
Thanks sir!
Very clever... Now please show us how to update it into a phase contrast xD
Excellent
Nice video bro.
Thanks
soooooo good thank you 🙏
thank u ...so help ful msg ...keep it up ..
Niceeee! This is a killer microscope 'hack'. Thanks!
Thanks!! I was researching here, it's not that expensive slide sheets to print on a laser printer (fortunatly I already have such a printer). I'll try printing some!
Works well after some time spent with fitting. I made one a couple years ago. How about polarizing for the DIY person?
Thankyou
here this one can be done also easyand cheap, just 2 polarising filters , 1 for the lightsource and 1 for the Eyepiece (CPL Filters can be found from 3$ each in the analog cameras section on ebay for example on ebay in your country or on ebay china)
@zazugee
7 жыл бұрын
that's not darkfield, it's phase contrast you're appearing to be doing here.
@michaelb.1860
4 жыл бұрын
That’s neither darkfield nor phase contrast but...wait for it...polarized light aka polarization contrast
Great vid! Good presentation, simple, inexpensive, effective! Tnx!!! rc
Sir you are a wizard
I make em from plastic bottle caps. Very rigid, no cost and easy to work with
The field can be darkened further by enlarging the shaded diameter. Can we use a similar technique for solar filters on telescopes?
Nice video ! Nice person.
Nice information, do you have any trick for inverted microscope to turn them in phase contrast such easier way?
olique illumination is better than dark field. Simply use LEDs putting them a bit aside on filterholder
@JohnHoranzy
6 ай бұрын
I have done that at low magnification. Now I have the cash for the real thing. Hope I am not disappointed with it.
omg we need this :D
Great, for the beginner to understand the case - super!!! What about polarization and DIC? I just bought a microscope and all that extra stuff is so expensive and i have no idea how to apply it :-) i am gratefull for help.
How do I know when I have the right diameter for the light block? Thx.
Thank you so much for your vid. Please give me some advice, I am growing some lactobacillus cultures (now trying to replicate some L. Reuteri). I would like to be able to see the action and make videoclips and pictures. Would an Omax 40-2500x work, is that enough amplification??? Good luck!!
My thumbs up came at the something something something part😂😂😂
Nice, i need help or direction. I want to see water clusters. Is thier a way.
I love you.
Hi Dan! What kind of microscope would you recommend a beginner that wants to view red blood cells under darkfield? Thanks for this DIY!
@haveaniceday7950
Жыл бұрын
This is my question as well. Did you ever find the answer?
What would be a good low cost scope for viewing live blood?
How do you film the microscope? do you have a usb lens or you put the camera in front of the eyepiece?
This might be a silly question but could you do something like this with a flashlight and jewlers loup. By covering the light from the flashlight with the black plastic and looking thru the loup? I know nothing about microscopes! I still have a lot of learning to do. I've been looking at gems as a hobby. I stare at them for hours with different magnification loups and a flashlight. I keep hearing about "dark field illumination" when I watch gem identification videos on KZread. Until I can get my own microscope I thought maybe this might be a very rough diy way! Thanks for the video... now when I do get a microscope I'll know how make my own dark field :) Plus it was funny! Nicely done!
I wonder if you could just paint the coin you use black and glue it to the transparent material.
Asewome!!!
Thanks. That´s only works in 5x and 10x. Someone know how to make that in 40x or 100x? I discovery a very easy way to do that , with 5x and 10x, with a specific kind of filter suporter: open the diaphragm and move the filter suporter to the right until you get the image. Will work too.
@gabrielyosei7565
3 жыл бұрын
I have the same problem with 40x
I tried this method to look at the spirochetes in my blood, and it worked well at 10x. But when I try it at 100x with immersion oil it doesn't work. The image shows up faded and looking like a poor lit version of a light field microscope. Do have any ideas why this would be, and how I can make this work for high power with immersion oil?
@wesremy
7 жыл бұрын
Phillycheesecreampie Cake u need a smaller cirkel for bigger magnifications
@wesremy
7 жыл бұрын
Phillycheesecreampie Cake no just kidding u cant get darkfield with high aperture objectives
@philipwilkin2667
7 жыл бұрын
Uhm... Why are there spirochetes in your blood?
@vortexor1
7 жыл бұрын
Did you get it managed to see good pictures of the blood in 100x?
Great idea, thanks for sharing. Wouldn’t it be quicker and easier to glue something opaque onto a single plastic sheet instead of colouring several sheets? Or am I missing something?
@Nihaikitty
5 жыл бұрын
I was wondering this as well.
@michaelb.1860
4 жыл бұрын
Yes that would work just as well - opaque disc should be as thin as possible
❤❤❤ ⚘️🌷⚘️. .Thank You Verry Much..🙏🙏😊😊😊👍👍👍
Meet Dan, He is a BAMF!!
So it’s not true or true because of strength that only Arizona & Nevada allow dark field microscopes
can't you just colour a paper circle in black and cover it in plastic circles
I gotta look at some mites from the dog
THANK YOU!!! I FINALLY FOUND SOMETHING ON MAKING A DARK FIELD FILTER!!! **Explodes.**
Brightfield
That guy used is Brain 🧠= 110% 😅
Somthing Somthing Somthing...not cheap...Ha!!! You can say that about so many things today...
I think 7 don´t like this Idea becauce they are selling Darkfield Microscopes .
What is with your odd enunciations ?
stop waiving your hands in the face of the viewer--it;s offensive
Moronic bordering on bonafide lunacy
brilliant. you saved me a few hundred dollars. thanks.
I am here from the future to tell you to buy Dogecoin.... you'll tank me later
Very very useful! Well done!! Thank you very much :)