How to prepare MS media//Stock preparation with some tips and alternatives//Tissue culture media

#plant_biotechnology​ #plant_tissue_culture #Murashige_and_Skoogs_Medium​
Watch this video to understand how to prepare stock solutions and make MS medium for plant tissue culture techniques
Click to watch
--Cell suspension culture: • Cell Suspension Cultur... ​
--Embryo culture: • Plant EMBRYO Culture//... ​​
--Callus induction: • Callus induction proce... ​​​​​
--Callus induction from seed: • Callus induction from ... ​​​
--The stages of in-vitro seed germination: • Stages of In-vitro see... ​​​​​​
--To learn the process of in-vitro seed germination watch this video: • Invitro seed germinati... ​​​​​​
-- To learn basics of sterilization techniques watch this video: • Types of Sterilization... ​​​​​​
-- To learn all about the Tissue culture lab, the instruments used watch this video: • Plant Tissue culture L... ​​​​​​
-- Learn how to fumigate the lab by watching this video : • Fumigation of Lab ​​​​​​
-- Visual tour of microbiology lab: • Microbiology lab and t... ​​​​​​
-- Visual tour of molecular biology lab: • Molecular biology lab ​​​​​​
-- Visual tour of a general life science laboratory: • Life science research ... ​​​​

Пікірлер: 65

  • @ashusingh6813
    @ashusingh68133 жыл бұрын

    You efforts show... How much you are interested in imparting knowledge. Good work

  • @strange9673
    @strange96732 жыл бұрын

    We appreciate you brother thank you for sharing your knowledge of the plant tissue culture techniques. Much love from Oaxaca, Mexico🤟🏽🍄🐍 -Dr. Strange

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Thank you for the support 🙏

  • @joyfull244
    @joyfull2443 жыл бұрын

    Hi I am also working on bacopa monnieri plants tissue culture and also cell suspension culture coincidence I see your video today from Karnataka ❤️

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    3 жыл бұрын

    Great to hear, wish you all the best 👏

  • @ExplosiveFists
    @ExplosiveFists Жыл бұрын

    so glad i came across these videos

  • @gethsamanegardens909
    @gethsamanegardens9093 жыл бұрын

    Very informative session brother...thank you

  • @prabhdeepkaur7777
    @prabhdeepkaur77772 жыл бұрын

    Great video sir 👍👍👍

  • @nxtlvlyt4466
    @nxtlvlyt4466 Жыл бұрын

    I love your videos 🙌😎 very informative and inspirational. One day I hope to work in a lab such as yours😄.

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    Жыл бұрын

    Means a lot 🙏

  • @ankitachowdhury5268
    @ankitachowdhury52683 жыл бұрын

    Thank you so much for your effort sir.......your all videos are very beneficial and informative ❤️

  • @reformerjugnetech7370
    @reformerjugnetech73702 жыл бұрын

    very informative

  • @abiabi-hx6vx
    @abiabi-hx6vx2 жыл бұрын

    Very usefull sir

  • @creatodayin4924
    @creatodayin49243 жыл бұрын

    Thank you sir really informative video 👌❤

  • @mudavathpadma4976
    @mudavathpadma49763 жыл бұрын

    Thank you so much for ur effort sir ur realy genious sir👌...u considered our request and updated video we r very thnk ful to you:)

  • @SantelWood
    @SantelWood2 күн бұрын

    Can you suggest the agencies supplying the equipments and the spice required for installing a most modern well equipped lab for Research purpose.

  • @anushkagupta4271
    @anushkagupta42712 жыл бұрын

    Thankyou sir.

  • @makemoney4386
    @makemoney43862 жыл бұрын

    Excellent video,sound,tropics And important is your effort.. Lots of thanks for your clear understandable communication.

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Welcome:)

  • @sanathekanayake503
    @sanathekanayake5032 жыл бұрын

    Yes ,thank you very much . good lesson as bigginers like me for tissue culture

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    :)

  • @AliHaider-fq8mx
    @AliHaider-fq8mx2 жыл бұрын

    Great video

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Thank you :)

  • @keerthic9692
    @keerthic96922 жыл бұрын

    Great explanation.Thank you Sir.

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    :)

  • @SantelWood
    @SantelWood2 күн бұрын

    I want to follow your class

  • @prabhdeepkaur7777
    @prabhdeepkaur77772 жыл бұрын

    Learnt many new things due to this video ✨✨ thanks a lot

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Im happy it was useful:) This makes my effort and hardships valuable 🙏

  • @amtd3808
    @amtd38083 жыл бұрын

    Sir please make video on preparation of Harmone stock solution for PTC..

  • @richisika4989
    @richisika49893 жыл бұрын

    Nice vedio.Please also make a video on estimation of beta-corotene

  • @bianconiglio1235
    @bianconiglio12352 жыл бұрын

  • @shravanigali4739
    @shravanigali47396 ай бұрын

    If we get individual chemicals of ammonia nitrate and potassium nitrate, should we take both of 35.5 gm separately

  • @SantelWood
    @SantelWood2 күн бұрын

    Since you told me that your lab is for post graduate students.

  • @darshanav5072
    @darshanav50722 жыл бұрын

    Really informative vedio...plz give me the details about ready made MS media power and which is the good brand?

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Himedia. I brought it through online.

  • @vawcreations5887
    @vawcreations58872 жыл бұрын

    I REALLY LIKE HOW YOU EXPLAIN. GREAT WORK. PLS MENTION THE LINK TO THE CALCULAION VIDEOS. THANKS AGAIN.

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Thank you kzread.info/dash/bejne/laNot5WvetCsgJc.html

  • @ZK292

    @ZK292

    Жыл бұрын

    @@Dr.Rakesh.B thank u sooo much sir...Very helpful

  • @restrictedendonuclease4340
    @restrictedendonuclease43402 жыл бұрын

    Hello sir I just did my 12th and I'd always had intrested in recombinant organism..so as I was getting bored I took leaves of citrus lemon and psidium guajava and isolated their protoplast ...and I'd mixed those protoplast in a tube containing polyethylene glycol (peg) for protoplast fusion ...now in a day I see that there is something green brown colur mass or coagulate is formed ...can you tell me what it is or if it's a hybrid protoplast how to culture it

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Im sorry,.i haven't done protoplast culture . I do not have practical exposure. Nevertheless u can read articles to know their culture conditions.

  • @restrictedendonuclease4340

    @restrictedendonuclease4340

    2 жыл бұрын

    @@Dr.Rakesh.B see I'm doing some incredible mutation in animal cell as well as plant ... I'm extracting gene which are responsible for cellular totipotency in plant cell

  • @restrictedendonuclease4340

    @restrictedendonuclease4340

    2 жыл бұрын

    @@Dr.Rakesh.B and I don't know much things practicality ... I would love if you could tell me something

  • @restrictedendonuclease4340

    @restrictedendonuclease4340

    2 жыл бұрын

    @@Dr.Rakesh.B how to extract gene from a plant cell like from agrobacterium tumificences or such kinda vectors

  • @mayankrawat2934
    @mayankrawat29342 жыл бұрын

    Any substitute for ammonium chloride, tell if any ??

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    There's no equivalent substitute for these compounds of MS media but you can alter for eg, u can take some other chloride sources like NaCl and ammonium sources like ammonium nitrate and check.

  • @TheOrganicSpade
    @TheOrganicSpade2 жыл бұрын

    Hi, I want to learn TC. Ive managed to find most ingredients except for PPM in india. Could you tell me where can one buy plant tissue culture ingredients in india?

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    We order online from Himedia

  • @TheOrganicSpade

    @TheOrganicSpade

    2 жыл бұрын

    Oh ok...but I'm sure they only sell to labs. Do you know of any suppliers who will sell to individuals?

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    No idea

  • @greenhut1642

    @greenhut1642

    11 ай бұрын

    What light is used, which is the container used. From where can I buy. Is it possible to at home

  • @neelamsingh8882
    @neelamsingh8882 Жыл бұрын

    Sir plz add the link from where u brought that, Supplement for Ms media.?? I need alternative for Nh4NO3

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    Жыл бұрын

    Buy from Himedia, browse in internet

  • @neelamsingh8882

    @neelamsingh8882

    Жыл бұрын

    Yes I had searched on HIMEDIA but Sir I can't find any product like this.. Plz sir can u tell the cas number?? Which is written on that bottle..??

  • @neelamsingh8882

    @neelamsingh8882

    Жыл бұрын

    Sir plzz 🙏 reply.. I want to know 😓 from where u brought this supplement for Ms medium..!! Because I have searched alot in Himedia.. Even I got ur cas no.. But then thus.. They are saying we don't have any products like this.. So plz 🙏help me sir.. Plz snd me the link or anything from where u brought this product..????

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    Жыл бұрын

    @@neelamsingh8882 I didn't buy, my lab had it already. And now I'm not in my lab anymore.

  • @neelamsingh8882

    @neelamsingh8882

    Жыл бұрын

    Then Sir plz.. Tell me any alternative chemicals name which I can use instead of ammonium nitrate..???

  • @ranajitghorui6376
    @ranajitghorui63762 жыл бұрын

    Sir all product Himedia?

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    2 жыл бұрын

    Yes. Our lab has Himedia but u can use other companies too

  • @poornimajoshi5537
    @poornimajoshi55379 ай бұрын

    chart is not clear

  • @kishorregmi2265
    @kishorregmi2265 Жыл бұрын

    sir could you send me the chart of ms media shown in your video

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    Жыл бұрын

    I do not have that chart dear. You can take it from Google.

  • @shravanigali4739
    @shravanigali47396 ай бұрын

    Plzz reply

  • @jamesjoseph5662
    @jamesjoseph56623 жыл бұрын

    Hlo sir I want to contact u

  • @Dr.Rakesh.B

    @Dr.Rakesh.B

    3 жыл бұрын

    Mail me at rakeshb96@yahoo.com

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