How to prepare MS media//Stock preparation with some tips and alternatives//Tissue culture media
#plant_biotechnology #plant_tissue_culture #Murashige_and_Skoogs_Medium
Watch this video to understand how to prepare stock solutions and make MS medium for plant tissue culture techniques
Click to watch
--Cell suspension culture: • Cell Suspension Cultur...
--Embryo culture: • Plant EMBRYO Culture//...
--Callus induction: • Callus induction proce...
--Callus induction from seed: • Callus induction from ...
--The stages of in-vitro seed germination: • Stages of In-vitro see...
--To learn the process of in-vitro seed germination watch this video: • Invitro seed germinati...
-- To learn basics of sterilization techniques watch this video: • Types of Sterilization...
-- To learn all about the Tissue culture lab, the instruments used watch this video: • Plant Tissue culture L...
-- Learn how to fumigate the lab by watching this video : • Fumigation of Lab
-- Visual tour of microbiology lab: • Microbiology lab and t...
-- Visual tour of molecular biology lab: • Molecular biology lab
-- Visual tour of a general life science laboratory: • Life science research ...
Пікірлер: 65
You efforts show... How much you are interested in imparting knowledge. Good work
We appreciate you brother thank you for sharing your knowledge of the plant tissue culture techniques. Much love from Oaxaca, Mexico🤟🏽🍄🐍 -Dr. Strange
@Dr.Rakesh.B
2 жыл бұрын
Thank you for the support 🙏
Hi I am also working on bacopa monnieri plants tissue culture and also cell suspension culture coincidence I see your video today from Karnataka ❤️
@Dr.Rakesh.B
3 жыл бұрын
Great to hear, wish you all the best 👏
so glad i came across these videos
Very informative session brother...thank you
Great video sir 👍👍👍
I love your videos 🙌😎 very informative and inspirational. One day I hope to work in a lab such as yours😄.
@Dr.Rakesh.B
Жыл бұрын
Means a lot 🙏
Thank you so much for your effort sir.......your all videos are very beneficial and informative ❤️
very informative
Very usefull sir
Thank you sir really informative video 👌❤
Thank you so much for ur effort sir ur realy genious sir👌...u considered our request and updated video we r very thnk ful to you:)
Can you suggest the agencies supplying the equipments and the spice required for installing a most modern well equipped lab for Research purpose.
Thankyou sir.
Excellent video,sound,tropics And important is your effort.. Lots of thanks for your clear understandable communication.
@Dr.Rakesh.B
2 жыл бұрын
Welcome:)
Yes ,thank you very much . good lesson as bigginers like me for tissue culture
@Dr.Rakesh.B
2 жыл бұрын
:)
Great video
@Dr.Rakesh.B
2 жыл бұрын
Thank you :)
Great explanation.Thank you Sir.
@Dr.Rakesh.B
2 жыл бұрын
:)
I want to follow your class
Learnt many new things due to this video ✨✨ thanks a lot
@Dr.Rakesh.B
2 жыл бұрын
Im happy it was useful:) This makes my effort and hardships valuable 🙏
Sir please make video on preparation of Harmone stock solution for PTC..
Nice vedio.Please also make a video on estimation of beta-corotene
❤
If we get individual chemicals of ammonia nitrate and potassium nitrate, should we take both of 35.5 gm separately
Since you told me that your lab is for post graduate students.
Really informative vedio...plz give me the details about ready made MS media power and which is the good brand?
@Dr.Rakesh.B
2 жыл бұрын
Himedia. I brought it through online.
I REALLY LIKE HOW YOU EXPLAIN. GREAT WORK. PLS MENTION THE LINK TO THE CALCULAION VIDEOS. THANKS AGAIN.
@Dr.Rakesh.B
2 жыл бұрын
Thank you kzread.info/dash/bejne/laNot5WvetCsgJc.html
@ZK292
Жыл бұрын
@@Dr.Rakesh.B thank u sooo much sir...Very helpful
Hello sir I just did my 12th and I'd always had intrested in recombinant organism..so as I was getting bored I took leaves of citrus lemon and psidium guajava and isolated their protoplast ...and I'd mixed those protoplast in a tube containing polyethylene glycol (peg) for protoplast fusion ...now in a day I see that there is something green brown colur mass or coagulate is formed ...can you tell me what it is or if it's a hybrid protoplast how to culture it
@Dr.Rakesh.B
2 жыл бұрын
Im sorry,.i haven't done protoplast culture . I do not have practical exposure. Nevertheless u can read articles to know their culture conditions.
@restrictedendonuclease4340
2 жыл бұрын
@@Dr.Rakesh.B see I'm doing some incredible mutation in animal cell as well as plant ... I'm extracting gene which are responsible for cellular totipotency in plant cell
@restrictedendonuclease4340
2 жыл бұрын
@@Dr.Rakesh.B and I don't know much things practicality ... I would love if you could tell me something
@restrictedendonuclease4340
2 жыл бұрын
@@Dr.Rakesh.B how to extract gene from a plant cell like from agrobacterium tumificences or such kinda vectors
Any substitute for ammonium chloride, tell if any ??
@Dr.Rakesh.B
2 жыл бұрын
There's no equivalent substitute for these compounds of MS media but you can alter for eg, u can take some other chloride sources like NaCl and ammonium sources like ammonium nitrate and check.
Hi, I want to learn TC. Ive managed to find most ingredients except for PPM in india. Could you tell me where can one buy plant tissue culture ingredients in india?
@Dr.Rakesh.B
2 жыл бұрын
We order online from Himedia
@TheOrganicSpade
2 жыл бұрын
Oh ok...but I'm sure they only sell to labs. Do you know of any suppliers who will sell to individuals?
@Dr.Rakesh.B
2 жыл бұрын
No idea
@greenhut1642
11 ай бұрын
What light is used, which is the container used. From where can I buy. Is it possible to at home
Sir plz add the link from where u brought that, Supplement for Ms media.?? I need alternative for Nh4NO3
@Dr.Rakesh.B
Жыл бұрын
Buy from Himedia, browse in internet
@neelamsingh8882
Жыл бұрын
Yes I had searched on HIMEDIA but Sir I can't find any product like this.. Plz sir can u tell the cas number?? Which is written on that bottle..??
@neelamsingh8882
Жыл бұрын
Sir plzz 🙏 reply.. I want to know 😓 from where u brought this supplement for Ms medium..!! Because I have searched alot in Himedia.. Even I got ur cas no.. But then thus.. They are saying we don't have any products like this.. So plz 🙏help me sir.. Plz snd me the link or anything from where u brought this product..????
@Dr.Rakesh.B
Жыл бұрын
@@neelamsingh8882 I didn't buy, my lab had it already. And now I'm not in my lab anymore.
@neelamsingh8882
Жыл бұрын
Then Sir plz.. Tell me any alternative chemicals name which I can use instead of ammonium nitrate..???
Sir all product Himedia?
@Dr.Rakesh.B
2 жыл бұрын
Yes. Our lab has Himedia but u can use other companies too
chart is not clear
sir could you send me the chart of ms media shown in your video
@Dr.Rakesh.B
Жыл бұрын
I do not have that chart dear. You can take it from Google.
Plzz reply
Hlo sir I want to contact u
@Dr.Rakesh.B
3 жыл бұрын
Mail me at rakeshb96@yahoo.com