How to calculate Km and Vmax values - Lineweaver Burk plot in Excel

This video explains about How to calculate Km and Vmax values - Lineweaver Burk plot in Excel.
Km and Vmax value calculation in excel from the enzyme kinetic data. Michealis menton constant (Km) can be accurately calculated from the lineweaver burk plot in excel.
Enzyme Kinetics: Lineweaver Burk Plot Explanation:
• Enzyme Kinetics : Line...
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Пікірлер: 94

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    @manny_k29883 жыл бұрын

    Absolute lifesaver. Super straight forward video, thank god I didn't have to sit through a 20min video explaining 20 billion different derivations of the the MM equation

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    @mlawliet61518 ай бұрын

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    @amandaouk8962 Жыл бұрын

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    @allexis58032 жыл бұрын

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    @abbiepalmer4547 Жыл бұрын

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    @user-bu6tk1mq7j9 ай бұрын

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    @mojzarea2 ай бұрын

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  • @isaiasv9925
    @isaiasv99252 жыл бұрын

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  • @BioResource

    @BioResource

    2 жыл бұрын

    Glad it was helpful. Consider subscribing for more such videos. Thank you

  • @blabiblabiblabi
    @blabiblabiblabi3 жыл бұрын

    Thank you so much. I did an experiment to determine Km. Now i know how do calculate it

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    @montserrathermosillo7802 жыл бұрын

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  • @BioResource

    @BioResource

    2 жыл бұрын

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  • @alphazenturbo
    @alphazenturbo Жыл бұрын

    Very straight forward. I needed to a L-B for Pchem and this was extremely helpful for making the graph in Excel.

  • @BioResource

    @BioResource

    Жыл бұрын

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  • @beehyve
    @beehyve Жыл бұрын

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    @dentedtester362 жыл бұрын

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    @daniellerochagiro34763 жыл бұрын

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    @BioResource

    2 жыл бұрын

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    @mr.legend3682 жыл бұрын

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    @JJH20042 жыл бұрын

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    @BioResource

    Жыл бұрын

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    @rosemaryjude26 Жыл бұрын

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    @wei2183 Жыл бұрын

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  • @rawyld
    @rawyld6 ай бұрын

    Thank you so very much.

  • @Not-Nur
    @Not-Nur2 жыл бұрын

    perfect video, thank you

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    @kelvineivo3 жыл бұрын

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    @BioResource

    3 жыл бұрын

    Glad it helped!

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    @hewyin61303 жыл бұрын

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    @michellemandelstam86333 жыл бұрын

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    @fizakhalid85377 ай бұрын

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    @LucasJensenOfficial3 жыл бұрын

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  • @BioResource

    @BioResource

    3 жыл бұрын

    Thank you.

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    @daniel98carvalho3 жыл бұрын

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    @BioResource

    3 жыл бұрын

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    @mariebrassart98683 жыл бұрын

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  • @BioResource

    @BioResource

    3 жыл бұрын

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    @GrayGhost_CV-68 ай бұрын

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    @anaa75952 жыл бұрын

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    @BioResource

    2 жыл бұрын

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    @BioResource

    2 жыл бұрын

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    @katsuno5916 Жыл бұрын

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    @jl2584 Жыл бұрын

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    @aishwaryaramkumar12212 жыл бұрын

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    2 жыл бұрын

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    @concurseiroentropy44833 жыл бұрын

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    @BioResource

    Жыл бұрын

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    @Koeliaify2 жыл бұрын

    The explanation is really very helpful

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    Жыл бұрын

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    @manonmclellan73042 жыл бұрын

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    @maxw59003 жыл бұрын

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  • @BioResource

    @BioResource

    3 жыл бұрын

    Welcome!

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    @nikitarashinkar18975 ай бұрын

    Thank you 😊

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    @captainlux409 Жыл бұрын

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    @rspeks94614 ай бұрын

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    @rspeks9461

    4 ай бұрын

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    @samanthac3233 жыл бұрын

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  • @BioResource

    @BioResource

    3 жыл бұрын

    Glad it was helpful.

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    @khadijahahmed1970 Жыл бұрын

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    @DeStIiNix99 Жыл бұрын

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    @user-nt2jf7oc2i3 жыл бұрын

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    @BioResource

    3 жыл бұрын

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    3 жыл бұрын

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    @kaoutar86563 жыл бұрын

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    @justflow7769 Жыл бұрын

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    @BioResource

    Жыл бұрын

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  • @danielhuelgas5355
    @danielhuelgas53553 жыл бұрын

    Thanks a lot!

  • @BioResource

    @BioResource

    3 жыл бұрын

    Welcome!

  • @songulates7833
    @songulates78333 жыл бұрын

    Thank you so much but i didn't understand , why is backward=0.5 periods for forecast?

  • @berkayadem7197
    @berkayadem7197 Жыл бұрын

    thank you very much

  • @BioResource

    @BioResource

    Жыл бұрын

    Thanks. Consider subscribing 😊

  • @zaidmaniar2899
    @zaidmaniar28992 жыл бұрын

    how did you get the initial velocities/product formed ?

  • @malleshgampa1956
    @malleshgampa19563 жыл бұрын

    Substrate concentration we have to take in mM?

  • @usmanabdulkareem7699
    @usmanabdulkareem76993 жыл бұрын

    Nice

  • @brianbridges8124
    @brianbridges81243 жыл бұрын

    hello, why do you bring the line to - 0.5, im wondering how u know what vmax is?

  • @zaysiz
    @zaysiz3 жыл бұрын

    Sometimes, I get Lineweaver-Burk equation as y=ax-b. While 1/Km is possitive, 1/Vmax is negative. Is it problem? Why is it negative?

  • @rakshamukhia7970
    @rakshamukhia7970 Жыл бұрын

    Sir, I do not understand but Iam gettingthe equation plotted in the graph with a ngative sign. where iam I flawed? y=10.192x-0.3494

  • @misbahhusolli
    @misbahhusolli2 жыл бұрын

    I found difficult when use this plot for bacteria fermentation kinetic, where the curve of 1/s and 1/u are not the same as the literature. the line of the curve is downward, Y = -ax + b, but the R square = 0,9 kind of. what if the result of this plot is downward curve, is it still can be use as a data to calculate Km?

  • @ximena5063

    @ximena5063

    2 жыл бұрын

    No

  • @foreverninteen8580
    @foreverninteen85803 жыл бұрын

    What is the unit of substrate concentration [S] here?

  • @solomonabioye1516
    @solomonabioye15163 жыл бұрын

    Thanks for the video but I still need help pls

  • @aelafalzabede6064
    @aelafalzabede60648 ай бұрын

    4.3002 how i can result this?? i dont know 😢

  • @zzziii-qr5io
    @zzziii-qr5ioКүн бұрын

    Why choose backward 0.5?????, can someone explain

  • @deepaksshanawad488
    @deepaksshanawad4882 ай бұрын

    How to calculate product formed values?

  • @hajrakhalil6737
    @hajrakhalil6737 Жыл бұрын

    Ak numerical solve krvna ta

  • @handlelector
    @handlelector Жыл бұрын

    2:15