How to calculate Km and Vmax values - Lineweaver Burk plot in Excel
This video explains about How to calculate Km and Vmax values - Lineweaver Burk plot in Excel.
Km and Vmax value calculation in excel from the enzyme kinetic data. Michealis menton constant (Km) can be accurately calculated from the lineweaver burk plot in excel.
Enzyme Kinetics: Lineweaver Burk Plot Explanation:
• Enzyme Kinetics : Line...
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@BioResource
2 жыл бұрын
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Thank you so much. I did an experiment to determine Km. Now i know how do calculate it
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@BioResource
2 жыл бұрын
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Very straight forward. I needed to a L-B for Pchem and this was extremely helpful for making the graph in Excel.
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@BioResource
2 жыл бұрын
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@BioResource
3 жыл бұрын
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@BioResource
3 жыл бұрын
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@BioResource
3 жыл бұрын
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3 жыл бұрын
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@BioResource
3 жыл бұрын
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@rspeks9461
4 ай бұрын
Got me through my chemistry assignment!
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@BioResource
3 жыл бұрын
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3 жыл бұрын
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3 жыл бұрын
Welcome!
Thank you so much but i didn't understand , why is backward=0.5 periods for forecast?
thank you very much
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how did you get the initial velocities/product formed ?
Substrate concentration we have to take in mM?
Nice
hello, why do you bring the line to - 0.5, im wondering how u know what vmax is?
Sometimes, I get Lineweaver-Burk equation as y=ax-b. While 1/Km is possitive, 1/Vmax is negative. Is it problem? Why is it negative?
Sir, I do not understand but Iam gettingthe equation plotted in the graph with a ngative sign. where iam I flawed? y=10.192x-0.3494
I found difficult when use this plot for bacteria fermentation kinetic, where the curve of 1/s and 1/u are not the same as the literature. the line of the curve is downward, Y = -ax + b, but the R square = 0,9 kind of. what if the result of this plot is downward curve, is it still can be use as a data to calculate Km?
@ximena5063
2 жыл бұрын
No
What is the unit of substrate concentration [S] here?
Thanks for the video but I still need help pls
4.3002 how i can result this?? i dont know 😢
Why choose backward 0.5?????, can someone explain
How to calculate product formed values?
Ak numerical solve krvna ta
2:15