future racism will be about if you were a red gatorade test tube baby or a blue gatorade test tube baby

@aegoni6176

13 күн бұрын

"You're one to talk! Mr came from egg juice"

@miskr3272

13 күн бұрын

Sounds an awful lot like someone out of blue would say.

@thomblueart8448

13 күн бұрын

well at least that makes more sense then the racism we have today!

@RedstonekPL

13 күн бұрын

such a vitamin water thing to say 🙄🙄🙄🙄

@ViktorRzh

13 күн бұрын

More like dark bear or white bear.

I do love that thought emporium is functionaly a crowd funded mad scientist

@hedgehog3180

9 күн бұрын

A new and more sustainable form of mad science.

@timothyhayes9724

3 күн бұрын

I should not have read that while eating b/c I nearly spat out my food cackling 😂

@AMPProf

3 күн бұрын

He makin zombies for sure

@krash2430

2 күн бұрын

you've created him ! Is he Doctor Frankenstein or the Frankenstein Monster ?

The missing ingredients are obviously Sugar, Spice, And Everything Nice, and *_CHEMICAL X_*

@darthplagueis13

6 күн бұрын

I'm pretty sure sugar is already included.

@teoeigi

6 күн бұрын

And then the perfect little girl was born

@rose.isnotavailable

3 күн бұрын

@@darthplagueis13I can’t imagine being satisfied with the sweetness of a Gatorade, tbf. they’d be majority spice and chemicals which is something but isn’t the ratio to be power puff girly enough (according to my own conjecture let’s be real for a sec lmfao-)

@knrz2562

3 күн бұрын

@@rose.isnotavailableerm huh uh ok 🫤 🤔 🙄 👍

@jeffsmith9384

Күн бұрын

we're trying to make something to eat, not give it the right to vote

One important thing to keep in mind, these cell lines were started and maintained in DMEM with FBS. This means that cells that preferred DMEM and FBS thrived, while those that didn't died off. With rapidly multiplying cells, it would likely be quite feasible to gradually wean the cells entirely off of DMEM and FBS and onto a substitute. This may be much easier than trying to generate cocktails of growth factors. Another key point is that the cells themselves often make the hormones they want. This is why many cell lines prefer partial media changes rather than complete media changes. This way, the cells always get exposed to most of the hormones they've generated.

@codiserville593

9 күн бұрын

Huh... I don't what you're saying but I kinda wish I understood more of what you are talking about now

@goo894

8 күн бұрын

@@codiserville593 I think they're saying that we could get the cells to evolve so they could survive in pure gatorade

@jacobwiens659

8 күн бұрын

They should definitely try and see if there’s a way to fully transition a cell line to a cheaper medium.

@PhycoKrusk

8 күн бұрын

@@jacobwiens659 For sure. The cost involved in obtaining the growth media are one of the biggest obstacles to bringing down the cost of production. (The cost of electricity is another, but we already have a lot of information on how to solve that)

@codiserville593

8 күн бұрын

@@goo894 well alright thanks for this possible translation. It's helpful

As a cell biologist, I'm fascinated. It seems it’s time to seriously diversify the contents of the refrigerator in our laboratory.

@noanyobiseniss7462

13 күн бұрын

You can bring red gatoraid to work, dilute and have some left over for your own mad scientist creations!

@calamatica

13 күн бұрын

Also...Our budgets are not unlimited((((

@NICK....

13 күн бұрын

no sir tax-man these energy drinks are 100% for Science!

@FleshWizard69420

13 күн бұрын

Monster Energy meat next

@llearch

13 күн бұрын

@@calamatica To be fair, it's not the labs that think the budgets are unlimited, it's the purveyors of the materials used. :-(

This is giving "how much sawdust can you put in a rice crispy treat before people notice?" And I love that.

@Meethejarate

12 күн бұрын

Yeah, it’s just “how much green dekara and coconut water can you put in a Petri dish before cells notice?”

@agiliteka

12 күн бұрын

although this is the opposite of the sawdust in a way, because adding more Green dakara makes it more tasty

@skunkjobb

10 күн бұрын

A very bad analogy. This has nothing to do with thinning out a product, it's a new way of producing meat without killing animals.

@Vileplume87

10 күн бұрын

@@skunkjobb I wasn't making an analogy I was making a goofy comparision

@coryzilligen790

10 күн бұрын

@@skunkjobb It's _LITERALLY_ thinning out a product. Did you not catch that they are using the Gatorade and other drinks as partial replacement for the cell media? Or all that various other stuff as partial or complete replacement for the serum?

It's crazy to think that common grocery store items could potentially replace some of the most expensive elements in the lab. Very eager to see where this leads to, and how it could potentially revolutionize lab grown meat.

@dakaodo

12 күн бұрын

Not entirely. There's almost always going to be minor or major compromises. You might be able to filter or separate out some of the undesirable elements, but every bit of that effort eats into the cost/time savings of using the retail alternative in the first place. But if something along that cheaper price point spectrum works "good enough" with 99.8% or whatever effective substitution, then yeah. For example, Thought Emporium mentioned two differences: the sports drinks significantly dropped pH balance out of the optimal cell survival range, and some trace elements in DMEM are situationally important for stuff like growing bones. I'm no cell biologist, but I'd imagine there are more nuances like these that they didn't mention. Another big one is quality control and precision to prevent variation. Two units of the same sports drink from different bottling plants or batches in the same plant could have a huge 0.2% variation in concentration of some elements. Not enough for a consumer to taste more or less salty, but could be enough to throw a precise lab process out of whack. A lot of the extra $100s of cost is for processes that ensure these error margins are orders of magnitude smaller. So the discount sports drink doping might be good enough for growing boneless McRibs or chicken nugget paste, but not for pseudo pork chops or beef ribeyes that need more realism. Good budget tier alternative for producing off-brand or generic synthetic meat, while the top quality synthetic meat will cost 2X more just to add the necessary 0.2% trace elements or whatever. Just like current consumer product price tiers. Or the discount stuff could work great for pumping out lower cost protein feedstock to feed to actual animal livestock that's raised for real meat. Not as environmentally or cost optimal as directly growing a high quality vat steak, but maybe it would be an interim technology that cuts cost inputs and greenhouse gas emissions by 10% or something, per pound of real beef produced. That's basically what industrial agriculture has been doing at a lower tech level for the past century -- grinding up undesired wild caught fish bycatch and waste parts from farmed fish to make fish meal, which they then mix into feedstock for more farmed fish or other livestock. Same for waste parts from butchered cows, pigs, chickens, etc that are fed back into the system to feed new animals. (and yes, this has been a source of increased system vulnerability to contamination, disease, and things like growth hormones recirculating back into the system as it approaches both higher efficiency and a slightly more closed loop of production)

@pukvandepettoflat7082

11 күн бұрын

Lab grown meat ..? Dont you mean DIY grown meat ? 😁😆

@Kiddio

11 күн бұрын

The reasoning is because those expensive mediums are specifically tested and the ratio of contents confirmed to not have anything additional in there to not throw off experiments. It’s the same reason anything “aircraft grade” is expensive. It’s also the same reason NIST sells peanut butter for like $400. You’re not paying for the item but for the paper trail to validate your results. This is especially important in healthcare research in the case of any kind of side effects or other issues later on. Stuff like this is definitely great to know, especially if you’re just doing some preliminary research into a topic, but if you’re publishing a paper then you should be using standardised ingredients/processes.

@tynj4173

11 күн бұрын

you can't make me eat it

@greycat5383

11 күн бұрын

Wouldn't be the first time something like that happened. A while back a lab tested using the old Shrinky Dinks toy to create patterns for use in microfluidics. It worked.

"Milk is just purified blood." I guess all mammals are purified vampires.

@deaddead698

5 күн бұрын

I mean-

@jaythewolf7216

13 сағат бұрын

just made me think of Dracula biting someone's booba . lady " help Dracula keeps biting my nips "

@quaxicron

Сағат бұрын

bruh...

Ah, splendid, we're getting closer and closer to finally finishing the ages old task of creating a Homunculus.

@guts60

13 күн бұрын

The medieval alchemists would be proud!

@FleshWizard69420

13 күн бұрын

Let's not crush this one with a book

@arcalranem6561

13 күн бұрын

homunculus is easy, though. Test tube babies already exist.

@cevatkokbudak6414

13 күн бұрын

That would be a fucking insane and I want to see it

@Tunkkis

13 күн бұрын

​@@FleshWizard69420 If it behaves, and doesn't spit acid at its creator.

Dude did not grew meat, he grew a pure gamer

@NICK....

13 күн бұрын

can't wait to have a gamer burger one day

@Somedude20282

13 күн бұрын

Gamer nuggets

@aeriumsoft

13 күн бұрын

I made a gamer grape????

@celestinemachuca2339

13 күн бұрын

Ew

@ValeBridges

13 күн бұрын

Perfect for the Doom Brain Bot

I'm happy that you looked into this as most organisms aren't too picky about what they ingest to survive and most of what we consume is either organic or derived from something that is. Like you said in the video: the biotech supply industry intentionally runs up the prices of otherwise inexpensive compounds simply because they know they can due to the grant money flowing into universities and other institutions. Without people working to innovate outside the current system: very little progress will ever be made on things that major corporations aren't interested in. Just the same as how the home computer hobbyists brought about the innovations needed for a truly digital age: biotech enthusiasts will need to learn how to progress outside of the (mostly closed-off) mainstream in order to make real progress in things.

“A cow is just a machine that turns grass into milk.” The design. Its very human.

Scientists around the world: Let’s use animal cells to develop treatments for patients Thought Emporium: *GATORADE MEAT*

@cola98765

13 күн бұрын

Thought Emporium is the mad scientist the world needs more of.

@porteal8986

12 күн бұрын

honestly the high cost seems to be one of the big barriers to practical lab grown meat, not to mention any other lab grown cells, so pretty useful

@NoAIStudios

11 күн бұрын

@@porteal8986 Exactly. An imagine being able to supply all your meat at the cost of some gatorade and an egg (so about $2 instead of the normal $15)

@duelviper9871

4 күн бұрын

@@NoAIStudios we re about 50 years away from every home having their own meat synthesizer in the basement that you just need to fill the vats with gatorade and eggs every month

Please call it "Brawndo Serum, it has what cells crave"

@Goodgu3963

13 күн бұрын

This is amazing and absolutely what it should be called.

@victoriouspancake

13 күн бұрын

Clicked on this video expecting the Idiocracy references...did not disappoint

@thethoughtemporium

13 күн бұрын

The plan was always to call it "brawndo: the cell growth mutilator, custard flavored"

@sjamesparsonsjr

13 күн бұрын

@@thethoughtemporium Perfect, let me know if you want me to design the shirt :)

@c1v1c2v2

13 күн бұрын

​@@thethoughtemporium the neuron optimized version can be Braindo

Hearing the phrase "meat laser" shook my entire world in a way previously not imaginable

@JustADiamondBlock

4 күн бұрын

"No John, your not allowed to be making meat out of Gatorade in the garage"

Why are academically interesting videos being demonetised whilst Scamverts are STILL being pumped into my home by KZread?

@DarthVader-ch4um

6 күн бұрын

Because KZread makes more money off of them, so of course they allow it.They are both scammers after all.

@MediocreContentEnterprise

4 күн бұрын

So true

When will you grow the doom playing brain in Gatorade

@Peppermynt.

13 күн бұрын

ultimate gamer brain

@eragonawesome

13 күн бұрын

I'm gonna guess "as soon as we figure out how to make it actually work" In fact, i would assume there's already at least some footage recorded of testing and such

@AageV

13 күн бұрын

If you grow it in Gfuel it will be esports ready.

@Peppermynt.

13 күн бұрын

@@AageV PERFECT

@tinkerduck1373

13 күн бұрын

It's got electrolytes! It's got what brains crave for.

"Only the Polytron reduces an entire mouse to a soup-like homogenate in 30 seconds."

@Javierm0n0

13 күн бұрын

Top ten cursed phrases of all time.

@arge7661

13 күн бұрын

A blender does the same

@mironbarabakh5970

13 күн бұрын

Don't the blades kill and cut the cells?

@hrishikeshaggrawal

13 күн бұрын

holy shit

@anteshell

13 күн бұрын

@@arge7661 It doesn't. Blender breaks the cells and make it incredibly messy barely soluble and un-cleanable paste. Way more harm than good.

Using Gatorade of all things to grow meat, just amazing. It just feels like an april fools joke or onion article "Mad scientist makes breakthrough cost reduction in artificial meat production, Sponsored by Gatorade".

Great video, but I also wanted to add some information about university research, for anyone interested 1. When getting grants Universities first take a portion for the use of facilities, this can go up to 50% but I believe 20-30% is standard 2. Assuming your lab isn't big enough for some major lab equipment like an NMR the university often has a communal one...that you must pay to use 3. The lab also needs to pay for graduate student tuition and wages 4. Now that the majority of the money has probably disappeared you now have to deal with whatever miscellaneous costs appear, maintenance of equipment, purchasing chemicals, traveling to scientific conventions Academia is basically a black hole that devours money

@ritishify

11 күн бұрын

Such a shame. But it is the world we live in, and money seems to be the best tool we could've come up with. It's both one of my favorite and most hated inventions, haha.

@Dylan-ln6qt

11 күн бұрын

Yeah it can be a pain, my advisor basically doesn't have free time with the constant grant applications he has to file, granted it is a fairly large lab

Pocari sweat can be found at most Asian markets both in the US and Canada, with many online retailers also shipping it from local warehouses. No real need to have it imported. Also it comes in its original powder form if you're worried about shipping weights. But all in all its effectively a Japanese clone of Gatorade made by Otsuka Pharma.

@Yadobler

13 күн бұрын

Me rn in Singapore chugging the $1.50 (usd1) bottle of PS

@Yadobler

13 күн бұрын

Also yes I wanted to mention they come in power form. I remember being in police cadet corps and our outfield ration included the powder. We mixed it into our water bottles that were brewing in the hot sun, and chugged it - horrible but delicious. Also had to make lunch using some cheap off-brand instant noodles, and I think some folks forgot to pour out water into the mestin for the noodles before mixing the PS powder. But anyways we opened a tin of sardines in tomato and threw that into the mestin of noodles and honestly, pretty good!

@theendoftheworldhasbeenqui2485

13 күн бұрын

I wondered why he imported it when I can get a bottle of pocari from a local asian store in London for a few pounds. I'd be surprised if you couldn't also in Canada

@ztheo2280

13 күн бұрын

@@theendoftheworldhasbeenqui2485 honestly it was probably just because its the best way to make sure you get the exact same product. yes its almost guaranteed to be the same locally but it might not be. Also i wonder if the powder might not be better because you could adjust the concentration if need be

@llearch

13 күн бұрын

@@ztheo2280 In hindsight (yay, I can see clearly now!) the powder form probably would have been better. Still... next cycle?

"After several hours, Joe finally gave up on logic and reason and simply told the cabinet that he could talk to cells and that they wanted Brawndo"

You absolutely killed me with the bit around 13:25. Top notch

18:32 Try combining the top performers to test if they can complement each other somehow. Maybe a mixture of some can have a more complete nutrient profile for the cells. Also another idea to try: "Soylent complete meal powder". It's supposed to be a balanced mixture of most nutrients the body needs.

new alchemy video just dropped

@adengoldstein5130

13 күн бұрын

Next project turning lead to gold

@crimsonqueen751

13 күн бұрын

@@adengoldstein5130 ...using mushrooms and baby cow juice

@grapesandsand3816

13 күн бұрын

@@adengoldstein5130 wikipedia/Golden_rain_demonstration

@sparking023

13 күн бұрын

If they whip out a large metal armor, we better be very worried

@_marshP

13 күн бұрын

Next project combining a dog and a little girl

A few friends in my department (USU Biological Engineering) are working on creating an FBS substitute using subcritical water hydrolysis. They take algae and put it under high pressure and temperature to break down the algae into metabolites cells can use. The goal was to produce a vegan media that Upside Foods could use for cultured chicken. Pretty neat!

@CaleGuthrie

12 күн бұрын

That's amazing

@andresmorera6426

9 күн бұрын

Hopefully it can be used for all sorts of cell culturing applications beyond the private sector... And that the recipe and protocol is open source

@Blimsky

8 күн бұрын

so a pressure cook it to a paste then feed it to vegans, sounds like the pink sludge in fallout

@andresmorera6426

8 күн бұрын

@@Blimsky I am a vegan, and I approve of this message.

About 25 years ago when I was a student, I was chatting to a biochemist about food and they mentioned that they'd never eat a custard-filled doughnut, because egg custard is a great growth medium and she'd seen how well it works for bacterial cultures.

its so cool being able to watch these videos now having done cell culture in lab. this channel was such a huge inspiration for me over the last six or so years

I'm a little confused - wouldn't the most important test here be a control with sterile water diluting the DMEM? To see if the alternatives are either really helping (like DMEM) or just not hurting anything while enough DMEM is available. Sorry if it's a dumb question.

@lucky-segfault4219

13 күн бұрын

Nah that's a good point. If DMEM can be diluted with distilled water and still work well, that's an instant drop in costs cuz every lab has access to distilled water. Heck, AC units make it as a side effect of cooling the air

@jetison333

13 күн бұрын

Actually I think your entirely right! I hope he responds to this comment

@theeyeofomnipotent

13 күн бұрын

Could be, but the 100% version with replaced fbs does remediate the results somewhat, Although yeah the data is better with dmem 0-100% with reverse osmosis distilled water dilution, it's not that complicated to do too, However the research from japan in video is probably more complete,

@person8064

13 күн бұрын

If my bio knowledge hasn't failed me, I think that's a negative control: a group that doesn't receive treatment and isn't expected to produce a result.

@NoNameAtAll2

13 күн бұрын

​@@jetison333you're*

If this channel has taught me anything it's that biochemists absolutely fkn love E.coli and use it a lot more than the average Joe not in the know would ever expect

@benjaminlamothe2093

13 күн бұрын

That's because they reproduce every 30 minutes, they are crazy efficient.

@benmcreynolds8581

13 күн бұрын

Yep from what I've seen is since they reproduce so fast, they can quickly see any effects, changes & then go from there.. But I'm no certified scientist

@am529

13 күн бұрын

there are a plethora of different strains, some of which naturally live in the human digestive system. Only a handful are harmful to people.

@markopolo1271

13 күн бұрын

@@am529 I know I'm more so just commenting on how E.coli is used far more in biochemistry than anyone not in the know would ever expect. I for one didn't know until I stumbled on this channel just how much it's used in this field of work.

@littlegrabbiZZ9PZA

13 күн бұрын

They're just overall pretty chill dudes. Very relaxed growth requirements, readily uptake DNA if zapped and/or sauna-ed a bit, don't stink to high heaven, they even come in nonopthogenic!

Guys are really about to put out an open-source generic brand DMEM. I'm interested now how Aquarius, another Japanese Pocari Sweat competitor, would do. There's also apparently now a "new" Green Dakara, would be interesting to see if it does any different than the regular Green Dakara. I can help answer part of why the nutritional yeast did so well: it's literally just dead cells. When we brew, we use yeast nutrient, which is honestly just mostly dead yeast. In fact, a quick and dirty way to make yeast nutrient is to just boil a packet of bread yeast for a few minutes. The dead cells contain a lot of the necessary building blocks for new cells to grow, for obvious reasons. It's really crazy to me that any lab can just casually say they can order some DNA to get printed. Imagine telling someone that 100 years ago.

@AR-yd2nd

10 күн бұрын

Not really open source, those brands are still proprietary. But cool af

@laurieb3703

5 күн бұрын

DNA? Wassat

Your channel is the most interesting thing I've found on KZread ever. I wish you success so you can keep working on the projects you want to do and for us to watch them

For processing the eggs, take a look at what was used for “albumen print” photographic processes. The literature is not the easiest to find, it’s mostly in century old photographic journals. The goal was to have a clean and optically clear protein gel into which silver nitrate grains would be suspended.

@rahulsharmajammu

13 күн бұрын

Prep for albumen print is basically what was done here. Take albumen, whisk till stiff, leave overnight, and filter out solids, 🤷🏾

@Caenef

13 күн бұрын

@@rahulsharmajammu Ah, glair! Seems to be pretty useful stuff for more than just illuminating manuscripts and gluing books.

really would not have expected beer to do so well...

@dr.spudies1555

13 күн бұрын

Much like your average dad over 40, cells just love beer

@MattRose30000

13 күн бұрын

it's what secretly keeps humanity alive for millennia

@dr.spudies1555

13 күн бұрын

I mean historically speaking wine, beer, and spirits are some of our oldest inventions.

@hammerth1421

13 күн бұрын

It's basically yeast broth, especially since the way they used it massively dilutes the alcohol.

@bernard832

13 күн бұрын

Brewing beer is cell culture. The main difference is that the spent media is the desired product.

13:15 this part reminds me of the Merengue sketch from Les Lutiers XDD

thank you for your passionate projects that you bless youtube with. this stuff makes me want to go into molecular biology

"When you think about it, milk is just purified blood" is NEVER a sentence I expected to hear

@josephcasebeer7683

8 күн бұрын

So are tears.

@hairymcnipples

6 күн бұрын

What? That's ridiculous. It's nutritionally fortified sweat!

@CharlieKell

6 күн бұрын

Don't think about the circumstances for the cows to produce milk then.

@feuerling

5 күн бұрын

Milk is just fancy sweat. Much more tasty than normal sweat though, luckily.

@GoldenPantaloons

5 күн бұрын

​@@feuerling How DOES normal sweat taste?

The comment count is already nearing a thousand so I don't know if anyone reads this, but wouldn't it have been more beneficial to use carbon dioxide or some acid that you could later precipitate out, to separate the whey from milk? Vinegar (mostly acetic acid) is a weak acid with small molecular sizes that can pass cell membranes, so even after neutralizing the pH, it would keep reacting inside and outside the cells and mess up things. At least that's why one should never adjust aquarium water's pH with weak acids. Doing so could easily end up killing the fishes. Only use strong acids (nitric, sulfuric, muriatic) for that, which leave chemically more inert acid residues. Not sure if any of the aforementioned ones would be suitable for this purpose though. That's why I suggested either carbon dioxide (at least it's the easiest to obtain and apply with sodastream, not sure if there are other acid forming gasses that have high vapor pressure) that could be mostly evaporated from whey by bubbling the solution with nitrogen gas (pumping just air would remove the CO2 as well, but it might also oxidize something important), or maybe there's some suitable acid that would precipitate during the neutralizing process, like oxalic acid + calcium hydroxide. Just the first few ideas that came to my mind.

@sneediumminer

13 күн бұрын

yeah acetate ion in humans is toxic so i cant imagine having a bunch of it floating around is good for the cells

@user-fz1nu9uy6q

9 күн бұрын

Now i want to see what happens if i soda streamed some milk xD

@nyyppa7956

9 күн бұрын

@@user-fz1nu9uy6q You'd get lots of foam, and coagulated proteins separating from whey. More importantly, I'm looking forward for your taste report, which is the, erm.. more interesting part 🙃

@pigsnoutman

7 күн бұрын

Dilute sodium acetate is extremely benign. It's used in blood transfusions and neonatal care, so it's probably close to harmless to cells.

@BobMcBobJr

6 күн бұрын

Could use dilute HCL. Make some more salt.

This work is so important to understanding how cells work. I’m so glad your videos are so easy to watch so people can get interested into science

Well done mate, love the videos. Always a good day to find a new one from you.

Now we need the reverse study: how much Gatorade can you replace with dmem before gamers stop seeing advantages Will dmem become the sports drink of top athletes?

@abyssstrider2547

3 күн бұрын

Lol

Fun fact: you can actually buy pocari sweat as a powder for dillution, I'm pretty sure that would cut down a little on shipping.

@thehuntermikipl1170

13 күн бұрын

A little?

@_Circus_Clapped_

13 күн бұрын

​@@thehuntermikipl1170 just dilute the powder in your medium, the powder is way stronger than whatever they put in the bottle

@thehuntermikipl1170

12 күн бұрын

@@_Circus_Clapped_ don't troll

Haha! Around 13:00 I was like, "Wow, adding sugar... ha, it's like he's making a meringue" [puts into piping bag] "Oh..."

I was planning to put the its what plants crave reference here at the end of the video, so glad you added the clip to the video!

8:49 I'm pretty sure the lab supplier sells them like that for consistency across experiments for being able to replicate experiments from papers. It's like how the ISO standard tea is like 500 dollars for a very bad and bland tea.

@DemsW

13 күн бұрын

That's 100% it, they pay to remove any variation factors. Same goes for most lab stuff.

@skorp5677

13 күн бұрын

Have no clue but tend to agree. Not required for exploratory stuff like on this channel though :)

@jonhinman2471

12 күн бұрын

Not sure who came up with removing all variations in order to 'baseline' ; it's a monocultural bland paradigm and actually eliminates any spice which extends life and expands consciousness. We need to understand and implement the interaction between rosemary, cell growth and antibacterial properties for instance as this among others can play a vital part in organic growth overall

@DemsW

12 күн бұрын

​@@jonhinman2471 Removing variation is the only way to be sure your results are the the product of the experiment and not some outside force. I've never seen anyone argue against that. Then in the experiment you can make as many/much variations as you want.

@okaydetar821

12 күн бұрын

@@jonhinman2471 It's just meant for running experiments, that's not meant to be a final product.

The joke is on you, providers have been using 70% gatorade on the medium they sell for ages to increase the profit margin.

@chyza2012

12 күн бұрын

that'd the make final mix 91% gatorade, and it still works well, you could probably get away with homeopathic concentrations of medium at that rate

@blar2112

12 күн бұрын

@@chyza2012 Homeopathy is a profitable business, expanding to grow mediums.

God I love how much detail they go into 😭😭😭 take my money just don't stop making this amazing insane content

My God I can't wait to see this upcoming stuff. Lab grown meat is something I'm definitely interested in and you guys just making some casually for fun really makes me optimistic.

Wow, that felt like 6 minutes. I was wondering "wait that's it??" as the video ended. You sure made it entertaining!

Holy shit the concept is freaking genius If this works on large scale And is refined The cost of animal tissue culture will reduce by a significant amount

@mbirth

13 күн бұрын

The cost might get reduced, but the market price will stay the same. That's how it usually works.

@GUGUthejoker

13 күн бұрын

Nobody wants lab grown meat

@chriswheeler6092

13 күн бұрын

People have already figured that out and started investing serious money. Now people are debating the Morality of it.

@charlethemagne5466

13 күн бұрын

​@@chriswheeler6092 raising and killing a cow is a lot more immoral than growing meat. One requires death and suffering the other doesn't, and no I'm not a vegan.

@Ger954

13 күн бұрын

@@chriswheeler6092let’s just hope it’s not harmful. Imagine a world where only the rich can afford natural healthy meat while the poor get sick with no other option

first time seeing your videos, really enjoyed! instant sub :)

This is fascinating Great job again mr mad scientist

The project of growing a complete gamer on gatorade is ongoing I see

I was reading papers some months ago looking for replacements for FBS growth factors and half gave up. Never thought of eggs and milk. Genius.

Man, you're freaking awesome! You make biotech so easy! These videos are awesome! But I do miss those older videos where you'd teach us making diy mods or even a whole new piece of equipment, like the nanodrop style spectrometer!

13:22 you cant do that to me man, its 3 AM, im still drunk and can barely focus on the video 😂🙏 dont make it worse please

Buddy you could have found a local Asian market for that pocari sweat & green one.. But props to burning that money for science!

@ThePenisMan

13 күн бұрын

Not everyone has a local Asian market. I know my town sure doesn’t, and it’s the second biggest in the state I’m in. The most Asian thing I can buy here is like… lo mein noodles from an actual Asian restaurant and offbrand pockys from Trader Joe’s

@solofdragons6446

13 күн бұрын

Thing is, Thought Emporium is a Canadian channel, and outside of major cities it can be very difficult to find ethnic markets that sell stuff like that. Even within the shops that do, it can be difficult. I know here in Manitoba, there's a store in Winnipeg called Oomomo, it specialises in Japanese products. I have not once seen Pocari Sweat or Dakari Green there, but even if i had the stock changes with whats popular in Japan. but Japanese products in general can be hard to locate here. Most Asian markets i've seen cater more towards Chinese and Filipino products as we just have a much larger population of Chinese and Filipino immigrants than we do Japanese.

@Shall0e

13 күн бұрын

​@@solofdragons6446inside London, Ontario, there are a couple of Asian Markets, and I'm decently sure they have those there, if he lives nearby or wants to make the trip, it's a good idea to check.

@rogerp.2442

13 күн бұрын

@@solofdragons6446He’s in Montréal, Pocari Sweat is 100% available here on some japanese/korean supermarkets

Fellow biologist here, I think this should be someone's master's thesis or student project and it is showing promise to be a startup one point. A big however, the only reason I believe that DMEM's can't be replaced by homemade recipes in academia is not the growth issue, more about replicability of the experiments. It's already so hard to follow protocols even though you use the exact brand, the exact amount indicated in the paper. Other than this, let's break the monopoly of lab suppliers lol.

@kokofan50

13 күн бұрын

Maybe the industry should move away from proprietary formulas to standardized formulas

@KanalBiyogen

13 күн бұрын

@@kokofan50 as the video says, more or less each DMEM contain the same ingredients however such small differences may create certain issues. The point here is the homemade recipes will complicate the issue even more and academia will probably won't like it.

@andresmorera6426

9 күн бұрын

Hell yeah, let's break those price gouging monopolies and cartels that make research unnecessarily expensive!!! Also, I agree small differences in cell culture media do cause issues. But in a hypothetical situation where labs are making their own DMEM from the same recipe, I believe from my experiences in cell culture that variability that already exists due to differences between lots of FBS is way more significant than the likely differences between homemade batches of DMEM substitute. I hope that sentence made sense.

This was a fascinating video. Although not currently studying in the field of biology, I am curious to know if a symbiotic culture like Kefir could make milk, or similar products, better growth media. Anyways, great video as always. Thank you for making such content free, it's been a very long time since I've been to a genetics lab as a student and I miss those days. Good luck with every project.

This is incredible work!!

Every day, I get closer to my clone army

@tr3vk4m

13 күн бұрын

you should probably throw those tissues away

@adora_was_taken

12 күн бұрын

elsewhere in the world, someone's working on a droid army

@ThatGuyRNA

12 күн бұрын

@@adora_was_taken that’s my buddy vin, I sure hope we don’t have conflict in the future

I guess feline bovine serum is like the real life equivalent of LCL from Evangelion.

@azertyQ

13 күн бұрын

fetal*

@KarachoBolzen

13 күн бұрын

​@@azertyQthe cat cows are taking over

@memejeff

13 күн бұрын

MooYow

@noob19087

13 күн бұрын

@@azertyQ Wow. That's hilarious, how did that get in there? I'm keeping it!

@Adshercott

12 күн бұрын

Yoga serum, but only the one pose.

this channel straight up is the most exciting thing on all of the internet. Keep it up man, you're gonna solve major world problems

Quickly becoming one of my favourite YT channels ♥

For a moment there I thought those cartons said "Concentrated concentrate" I was thinking "That must be some INCREDIBLY concentrated...whatever the fuck it is!" 🤦😅😑 I need sleep, but AFTER this awesome vid!

@FleshWizard69420

13 күн бұрын

I was concentrating on the concentrated concentrate too

With regards to using milk, about a hundred+ years ago physicians did try using milk as a blood replacement for transfusions. It didn't work, but I thought it was interesting to consider that other people throughout history were on a similar path.

@CAMSLAYER13

13 күн бұрын

You can use coconut milk a blood plasma substitute in a pinch

Awesome video. I've thought about trying something like this a lot, although the closest I ever got was adding some Jello powder to my cell media. (All the cells died.) The story of how the standard cell culture media were developed is fascinating. I recommend "Cells and Tissues in Culture" by E.N. Willmer (1965). It's really interesting to get a perspective from a time when the field was just starting to mature and to see what did and didn't work. There was a lot of trial and error in the early days much like you're doing in this video. (Gatorade didn't exist, but sea water was a recurring theme.) For a much shorter read, there's Harry Eagle's original 1959 paper "Amino Acid Metabolism in Mammalian Cell Cultures" (Science, New Series, Vol. 130, No. 3373 (Aug. 21, 1959), pp. 432-437).

Congrats on what you’ve learned. Excited to see what it means for the future

Months of patreon support to pay for a teaspoon of baby cow juice, worth it.

God, i love science. >laberatory technicians developing a nutritional aid for artificial cell growth in labs _"Wait... What if we just swap it out with Japanese Gatorade?"_ *>actually works

100% yes to this whole video. Going to be hugely beneficial when I move into cell work.

This is awesome. Really awesome. It’s incredible how closely cooking meat and creating meat are related. Side note, it also makes me feel more at ease about the concept of consuming lab-grown meat.

I think testing electrolyte powders would be an interesting next step, there are unflavoured ones that can be purchased in bulk for very cheap( $20 for 250L). Would remove any additives from the equation.

@brylozketrzyn

12 күн бұрын

At that moment just go for pure additives. Electrolyte drinks are mostly salt, sugar and flavour, sometimes with a small amount of potassium chloride

@Aqoric

12 күн бұрын

@@brylozketrzyn Proper rehydration formulas often contain BCAAs and other amino acids as well as

@brylozketrzyn

12 күн бұрын

@@Aqoric more complex ones for sure, but we can get aminoacids from many sources. It is about balancing nutrients and hormones.

@Aqoric

12 күн бұрын

@@brylozketrzyn Absolutely, my original point was simply for large scale production it would likely prove cheaper

I was lucky enough to work in a chemical plant as an operator, yhe company was the type you'd take a concept to, and they'd do the R, D & P... and never before in my life have I been more regretful than then... the fac I didn't study chemistry GUTS me.. or any the sciences tbh. Literally EVERYTHING is chemistry. And it blows my damn mind.

@StoneBox_761a

13 күн бұрын

blud is bluffing hard with that grammar, he did not work in a chemical plant yall.

@LordDragox412

13 күн бұрын

@@StoneBox_761a He was just an operator making the shine, the rest of the gang was doing science on improving the taste to sell it for more.

@StoneBox_761a

13 күн бұрын

@@LordDragox412 regardless, i dont think he worked there with that grammar, the requirements to be there are immense.

@LordDragox412

13 күн бұрын

@@StoneBox_761a He used to have perfect grammar, but then he drank some heads. That's why he's regretful he didn't study chemistry in middle school. Quite a sad story.

Been waiting for this video after seeing the plates on X, damn hilarious i love it.

Glad to see you doing research on fbs replacement as well. Even if it's not completely synthetic. It could drive the cost cheaper and more ethical.

I think the Vitamin water ingredient responsible for the cells not growing may have been vitamin A.

@noi0124

13 күн бұрын

I think it's the kiwi curse. Even jello doesn't work with kiwi.

At least 2-3 states have, or are about to, ban the sale of cultured meat products. So what we need is a simple, affordable "kit" to grow meat at home. Something akin to gardening, mushroom cultivation, or home brewing. I feel like we're watching the development of such a kit with every new video here! I would love a simple kit/machine where all I'd need to do is subscribe to some kind of service for regular deliveries of raw materials, nutrients, etc., plop those into a machine like an inkjet cartridge, and grow cheap, infinite steaks at home. Also, kindof shamefully curious to sample my own cells and grow some my own "long-pork-belly." Honestly, I bet I'd be delicious...

@sparking023

13 күн бұрын

I don't think that will ever fly because it's not just the "affordable kit" that's involved in this. It's also all the equipment required and the sterilization and handling protocols required to grow meat tissue in labs. This team is trained in that area, and even then they're prone to mistakes because that's how it goes. So letting the average Joe meddle with this sounds like a good recipe for an outburst of salmonella or something like that. I'm not a chemist or a biologist, but with my experience in IT and programming I can attest: the hardest thing to do is making your process user-proof. Someone *will* find a way to screw things over, and in the case of foods, the responsibility falls back on the distribution company.

@skorp5677

13 күн бұрын

Wait, let me guess: The states trying to ban this are also full of right-wing, book-burning, cucumbers?

@badflamer

13 күн бұрын

@@sparking023 Off the top of my head, I'd ask if you knew anyone who ever sued hasbro for their kid getting food poisoning from an Easy-Bake Oven cake. Or, alternatively, sued betty crocker after using unrelated salmonella-infected eggs in a cupcake batter and let their kid lick the spoon. It seems to me that whatever products would be made of a kit like this would fall under the same kind of legal classifications. As long as the obvious, instructed, and prescribed usage of the kit and its supplementary products is safe, user mishandling would be seen as exactly that, and not tie back to the kit distributor.

@anonduckduck

12 күн бұрын

⁠@@sparking023tbf (a small amount of) people (non-commercially) can their own food which also requires massive precaution in handling It wouldn’t be in supermarkets but I could see a world where it becomes a culinary niche as opposed to a home lab niche

@SubLordHawk

11 күн бұрын

Ban the sale? Just how much did the farming lobby -bribe- _ask nicely_ for that?

Bought the hoodie ! Keep on the good work guys !

As a (former) lab technician who worked in cell culture for years, this is incredible I never liked openned a bottle of FBS (or baby cow juice, I love this name) but I never though about changing it with Gatorade

Aw sweet man made horrors within my comprehension, thanks to this awesome channel!

18:25 "Maybe the cells wanted more uMAMI?" hahahahah😂 so great.

The best part of this sort of research is: Your team is currently involved in food production that Will be used on a future space station. I Love It! This has truly sparked my own curiosity… Time to consider converting my own lab for said tests. 🤔

This is the first video of you that i have watched and i am astonished

This video makes me think about ameture orchid seed growing. The book "growing orchids from seed" (Seaton and ramsay 2005, Kew publishing) Actually lists a recipe for cell medium which involves a blended banana (p.g 75)

@fisrtnamelastname3083

13 күн бұрын

Orchid, organ, potato pataya

@mrslinkydragon9910

13 күн бұрын

@fisrtnamelastname3083 well growing orchids from seed is akin to cell culture due to how orchids have evolved. The seeds don't have an endosperm (they are more akin to spores than seeds) and rely on mycorrhizal fungi to provide the developing seedling with nutrients. When growing them, you have to put them in a medium to recreate the conditions. As a result, you need to work aseptic!

@fisrtnamelastname3083

8 күн бұрын

@@mrslinkydragon9910 wow! That's really cool! Never thought I would learn such a fact by making an awful joke lol

@mrslinkydragon9910

8 күн бұрын

@fisrtnamelastname3083 oh I didn't get the joke, I just like saying facts

I would have loved to see a control with water or saline used to dilute the mixtures (even though that would probably lead to death pretty fast, but it would kinda put into perspective how beneficial the gatorade ect were)

What! The mummy video got demonetized? That was one of the coolest videos, bought your papyrus mummy guide after that video.

that was some really cool results. I noticed the best fbs 100% replacements were not also the best at 50%. I'd be curious to see the fbs test with 75% added for an extra data point

bruh 13:20 was such a genius prediction from whoever edited or scripted the video! i literally zoned out and got caught spot on, i enjoyed that !! thats a whole different layer of humor

Finally, I can make my own Meatcubator!

It's probably not just the composition in the drinks that matter, but also the amount and concentration of them. Green Dakara somehow struck gold in maintaining a good balance of all its ingredients for this task, only needing 10-30% of DMEM to fill the missing pieces. This also means there's something that the cells require that is overabundant in the original DMEM solution, considering you can substitute it with 90% pH-balanced Green Dakara. I think the next step to further reduce costs is to analyze what exactly you're missing from Green Dakara that that last 10% of DMEM has. If you can pinpoint the exact few things Green Dakara doesn't have that DMEM has, you can eventually stop needing DMEM entirely. As for the FBS, honestly 50% is already great to stamp down costs, but specifically for whole chicken eggs you might want to try growing chicken meat cells with it and see if it'll have better results. They're both chicken, so I'm sure the familarity should give better results; the whole point of a chicken egg is to grow chicken cells, after all.

I didn't realize you from Canada, as a fellow Canadian I recommend checking out asian grocery stores, especially chains like T & T as they are likely to carry Pocari Sweat and Dakara. They don't always have it available, as it tends to be a more seasonal import during the summer, but they do carry it!

This was the best video ive seen all month. Cant wait for part 2!

I feel like mushrooms would have been a good candidate for one of the substances to test. Either the mycelium or the fruiting body.

@Br3ttM

13 күн бұрын

Yeast is fungus, and was tested. An actual mushroom would be too hard to liquify.

@Kisoku

13 күн бұрын

@@Br3ttM Yes, yeast is fungus, but it's single cell organism. In my mind multi cell organism would have more need for things requiring the growth of cells. It's true that mushrooms are not liquid and that might be a problem as they are living beings on their own 🤔 liquid cultures are a thing though. I'm not a biologist or a chemist so I can't comment if mushrooms are useful. Even milk has bacteria if it isn't pasturized.

Currently visiting Japan and tuned in. It was crazy to see how well the Green DaKaRa worked! No wonder they all live long out here; they're consuming growth medium!

This is... genuinely incredible? At the very least, it lays the groundwork towards GREATLY reducing the cost of critical research and development, which is obviously a worthy endeavour in and of itself. Fascinating work as always!

A part of me wonders if you can take the best preforming things and basically use it as a recipe for cell production. Theres also a good video by Nighthawkinlight about an experimental procedure to change a lot of variables at once and analyze the results without having to change one factor at a time and doing a ton more work. Doing something like that as a followup could be interesting. Also keep in mind that doing this at home means that you don't need perfect results just a "good enough" standard and I'm sure this can do that. Also maybe in the future you can look at doing it entirely from scratch like you did with the soup.

20 seconds in and it's already cursed.

Thank you for this lovely Meringue recipe, i'll make sure to follow it

Dude, this channel is Insane, for real your did a Merigue