would be able to help me clear my doubts that what factors decide the type of calibration either linear or logit calibration? and also what determines number of standards required for an assay calibration?
@teresabixby1136
2 жыл бұрын
The calibration line should be determined by the model you expect the data to fit. For absorption spectroscopy we use Beer's law as a model (A = ebc) which is a linear model so therefore the fitline of the calibration curve should be linear (As concentration increases, Absorption increases proportionally.) The number of points will determine the precision of your calibration. For a linear fit you would need no fewer than 3 points, but the more data points you include, the more precise the trendline is (the smaller the standard deviation).
@misharialashban7335
Жыл бұрын
@@teresabixby1136 where can i find the link where you demonstrate how to plot using excel ?
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Thank you
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would be able to help me clear my doubts that what factors decide the type of calibration either linear or logit calibration? and also what determines number of standards required for an assay calibration?
@teresabixby1136
2 жыл бұрын
The calibration line should be determined by the model you expect the data to fit. For absorption spectroscopy we use Beer's law as a model (A = ebc) which is a linear model so therefore the fitline of the calibration curve should be linear (As concentration increases, Absorption increases proportionally.) The number of points will determine the precision of your calibration. For a linear fit you would need no fewer than 3 points, but the more data points you include, the more precise the trendline is (the smaller the standard deviation).
@misharialashban7335
Жыл бұрын
@@teresabixby1136 where can i find the link where you demonstrate how to plot using excel ?