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Nicely elaborated ...Thanks Sir!!

@bioinfoxpert

3 жыл бұрын

Most welcome!

Comprehensively explained.

@bioinfoxpert

3 жыл бұрын

Thanks

Very informative.

@bioinfoxpert

3 жыл бұрын

Glad you think so!

Learn a alot !Thank U so much Sir

@bioinfoxpert

3 жыл бұрын

So nice of you

@bioinfoxpert

3 жыл бұрын

If you want to learn Bioinformatics from scratch then take our course on Udemy Course Link: bit.ly/2SFH0fz The course is with 30 days Money Back Guarantee

Well explained 👌

@bioinfoxpert

3 жыл бұрын

Glad you liked it

Lo amo caballero

@bioinfoxpert

Жыл бұрын

We love you too. Many thanks for comment

thank you so much

@bioinfoxpert

4 ай бұрын

You're welcome!

It's very useful, just the video quality got a little problem, but congratulations and thank you

@bioinfoxpert

3 жыл бұрын

Sorry about video quality. Can you please let us know which think is lacking in video? We really want to improve the quality and content of our video tutorials? Many thanks you watched our video and highlighted the issue

@bioinfoxpert

3 жыл бұрын

If you want to learn Bioinformatics from scratch then take our course on Udemy Course Link: bit.ly/2SFH0fz The course is with 30 days Money Back Guarantee

omg, I know this trick after graduated, we don't need to lineweaver burk equition to determine the Km and Vmax value right?

@bioinfoxpert

2 жыл бұрын

Yes you are right

To calculate enzyme concentration [E] in Kcat calculation. Kcat = Vmax/[E] Example: Vmax = 200 umol/mg/min Enzyme concentration = the amount of the enzyme used in the assay or reaction from Vmax - its 1 mg. so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg) lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol (1 mole = 1000 mmol, 1 mmol = 1000 umol) 1 mg of enzyme = 1/28 umol = 0.0357 umol so concentration of enzyme [E] = 0.0357 umol/mg Kcat = Vmax/[E] =200 umol.mg-1.min-1/0.0357 umol.mg-1 Kcat = 5602 min -1

@bioinfoxpert

Жыл бұрын

Dear Sreeivas, Many thanks for your reply.

@Sree613

Жыл бұрын

@@bioinfoxpert Welcome and I thank you for your useful video to calculate kinetic parameters.

@srimantadash7435

Жыл бұрын

Thank you so much sir 🙏

sir, should we suppose the value of Et like yu did or we have to find it out?

@bioinfoxpert

3 жыл бұрын

Its 0.5 M for each

@joeyyjoy6655

2 жыл бұрын

@@bioinfoxpert so the value is fixed to 0.5M or we have to find out depending on the question?

Sir,how to calculate the enzyme concentration. My enzyme is 1000U/mg.can you help me.

@Sree613

Жыл бұрын

To calculate enzyme concentration [E] in Kcat calculation. Kcat = Vmax/[E] Example: Vmax = 200 umol/mg/min Enzyme concentration = the amount of the enzyme used in the assay or reaction from Vmax - its 1 mg. so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg) lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol (1 mole = 1000 mmol, 1 mmol = 1000 umol) 1 mg of enzyme = 1/28 umol = 0.0357 umol so concentration of enzyme [E] = 0.0357 umol/mg Kcat = Vmax/[E] =200 umol.mg-1.min-1/0.0357 umol.mg-1 Kcat = 5602 min -1

How did you get the total enzyme concentration

@Sree613

Жыл бұрын

To calculate enzyme concentration [E] in Kcat calculation. Kcat = Vmax/[E] Example: Vmax = 200 umol/mg/min Enzyme concentration = the amount of the enzyme used in the assay or reaction from Vmax - its 1 mg. so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg) lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol (1 mole = 1000 mmol, 1 mmol = 1000 umol) 1 mg of enzyme = 1/28 umol = 0.0357 umol so concentration of enzyme [E] = 0.0357 umol/mg Kcat = Vmax/[E] =200 umol.mg-1.min-1/0.0357 umol.mg-1 Kcat = 5602 min -1

lov u

@mauriciomoyaburdiles3590

Жыл бұрын

increible

@bioinfoxpert

Жыл бұрын

Love you too

@bioinfoxpert

Жыл бұрын

Thanks

I need some help with my data..can you help me??

@bioinfoxpert

3 жыл бұрын

How can i help you please let me know

@RajnandaniKashyap

3 жыл бұрын

@@bioinfoxpert I have my data for multiple substrate reaction. I am not able to analyze my data. I need help to understand few basic concepts. Can you share your email address so that i can share the data?

@bioinfoxpert

3 жыл бұрын

Datascienceandbioinfo@gmail.com

@bioinfoxpert

3 жыл бұрын

Please feel free to contact me

@RajnandaniKashyap

3 жыл бұрын

What is effective molarity? How do we calculate kuncat for a reaction?

It's Menten. Not Mental! 😂

@bioinfoxpert

3 жыл бұрын

Thanks for corrections

@bioinfoxpert

3 жыл бұрын

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