Wonderful job, systematically created video.

Your hand pouring technique is incredible bravo.

Thank you so much!!! This was very useful for my IBDP extended essay research !!!

@saimedhansh8835

11 ай бұрын

Sameeeeeeeeeeeee

very good and detailed described video.. if you have other assays, please share.. will be so useful. Thank you for a such a nice video

So great, but it will better if u also explain all detail. How to make standard curve, how many solution and solution to dissolve to make xx concentration in standard curve. I really need the detail

The Good results affirmed your great work .

thank you so much for your work, it is really a great help to me.

Can you please mention the reference for the protocol.

@riyariang2273

2 жыл бұрын

I hv the same question . Did u get the reference ?

@Shrisha-vv6jd

3 күн бұрын

Same. Did you get any references?

Thank you. It's very useful🙏🏻🙏🏻❤️❤️❤️

Thank you for the informative video. I hope you don't mind me using this to demo to my students. Thumbs up!

What about ascorbic acid..when is it used? And what is the control sample that has been used during spectrophotometric assay??

Thanks a lot for sharing the video. It's verw wll prepared.

Thanks alot

wonderful thank you

the referece of this principle please.

Thank You very much for the video, I don't see you using ascorbic acid and you listed it in your material to use

Could you mention the refference to the protocol, please?

Can you brief me how to carry out DPPH studies for topical cream

Great work 💪

Please mention the reference

What is the use of ascorbic acid? It is mentioned in the materials list but didn't appear anywhere else in the video.

Great Video! Could I ask for the references of this protocole? I am combined also about the use of ascorbic acid?

@elijahjadiel1524

3 жыл бұрын

a tip: watch movies at flixzone. Been using them for watching a lot of movies recently.

@juliokylan3775

3 жыл бұрын

@Elijah Jadiel yup, I've been using flixzone for since december myself :)

can someone explain for me where concentration in the result table from? thank youu

4:19- sample+methoh 5:22- each sample 1ml mixed with 3ml dpph 6:09- make up with methoh

@kvlogs3212

11 ай бұрын

What is amount of methanol added to the extracted solution

great video keep it up

Fabulous video... Plz share FRAP and MCA also in next videos

Amazing 👌

great video.looking for ur more videos also i need a video for well diffusion method for antibacterial actvity.

In table there is a column concentration mg/ml. Whose concentration doest it refers?

@oneday8287

2 жыл бұрын

I have the same question, please give me answere??

Thank you so much, can someone give me the reference please?

Good job

Thanks

What is powdered food sample? What is it in?

Thanks for the methodology. However, the results in the table at 7:53 are not the same as in the final graph. If we use the data available, the R^2 of the adjustment is only 0.843. The inhibition values are also incorrects in relation to the absorbance values. Why?

@simplywendy7216

3 жыл бұрын

Because he didn't describe his standard preparation. Calculations are based on results from standard curve which we don't know because he didn't show it

@iftekhar_k

Жыл бұрын

%Inhibition curve in not a linear curve as far I know

4 mg of DDPH in 100 ml of methanol does not refer to 1M. It is too far from being so.

when you were making the solution series..you took 1ml, 2ml, 3ml and 4ml of the sample extract..but how much methanol did you add to it?

@MahmoodHashem

4 жыл бұрын

he completed it to 10 ml (the sign )

Can anybody explain why his calculated IC50 value is 25 mg/ml while in the table it shows 65% Inhibition at 20 mg/ml?

You don't need a standard ? Usually for antioxidant we take ascorbic acid standard... I'm new to this and kind of confused. Help me

great

Hello, good video, a question, where did you get the reference for the laboratory guide? HELP

what is purpose of ascorbic acid please? because you mentioned to ascorbic acid in materials .

prepararon una solución a 0,1mM no a 1M..... con eso si toman 4 mg

Can you plz give us the reference of your protocol....it would be of great help

You mentioned acid ascorbic but there isn't in video please can you tell me how you used it? and please here your control was it only dpph +methanol ?? I need the protocol

Any refrence

I want cite this vedio, can u give link of this article where it from taken

In earlier video, you write ascorbic acid in materials, but i cant see where or when you use in tutorial.

@something.diff.

8 күн бұрын

Ascorbic acid used as control

@something.diff.

8 күн бұрын

Ascorbic acid used as control

Hi. Im a bit confused where does extracted solution come from, the one that you used to make dilutions? Thank you

@deenmediarecords4575

4 жыл бұрын

ı think it is the filtered solution from the test tubes.

@ranbirkumar5813

Жыл бұрын

Filtered solution

@tnehshuxin9530

10 ай бұрын

@@deenmediarecords4575 the filtered solution is come from one food sample only right?

Good

Reference please?

Dpph a simple assay/antioxidant a big term

haii can I know if we use a sample from a liquid ( fruit juice) that same method or different method ??

Will you share the research article??

Concentration ka column samja dijiye plz

How could we determine how many mL of solvents to put into the sample?

can I know why it needs ascorbic acid for this experiment?

Great video! May I ask if you combined the extracts of the 2 samples?

What did you use as your standard?

how do you calculate the concentration?

@arshidsyed6543

2 жыл бұрын

Are you preparing 1M or 0.1mM DPPH...I think it is 0.1mM DPPH you are preparing...

?plz the color yellow is obligatory te confirm the effect or not

what did you make in sample, and negative control

Sir Please provide me flowchart of dpph assay, please🙏🙏🙏🙏

Which lab is this (location/name)? I really liked your antique centrifuge and test tube stand.

@wings4749

2 жыл бұрын

Wayamba university of Sri Lanka 🇱🇰

Hello, how did you get 1ml, 2ml, 3ml, 4ml and 5 ml for the solution series?

7:54 Calculate Inhibition% by hand i.e. ((0.301-0.284)/0.301)*100%=5.65% not 5.86%. All values of Inhibition% are incorrect.

1:43 1mM DPPH ?

@salmamskine8220

4 ай бұрын

0.1mM

Reference??

excuse me I have a question, the sample showing at the beginning, can be in liquid form???? I would be very gratful if you would answer me

@bhaveshdonda2178

3 жыл бұрын

yes it can be in liquid form so that eliminates the whole process of extraction and then you just have to work from the making the series dilution process.

May i know which food sample you used in your study

Hi. When is it used ascorbic acid?

Which book you can get procedure ..name pls ...

where is ascorbic acid function n method ?

Any other solvent than 99% methanol that can use???

Hlo please provide any reference link

Where is the standard solution?

Can you please share the %Inhibition for iceberg lettuce using the same dpph assay?

@grantgabrieltollo9866

2 жыл бұрын

😂😂😂

What is the blank in the spectrometer?

DPPH 4 mg is real at that weight??

Please tell us the scientist name whose methadology you used for doing this experiment... please tell us,its so necessary to do this experiment.please text us the reference.please

@dienkimotaung7167

4 жыл бұрын

Hi did you get the references?

what is the blank composition

What is control? Is it sample abs without dpph or dpph only?

@aproblem6ix889

5 жыл бұрын

Alip Tanpanama it’s the one with ddph and ethanol without your sample

@rabiyamehandi6354

5 жыл бұрын

control is DPPH abs.

what you take blank solution?

Sir please send dpph data sheet

are samples soluble in DPPH solution?

Hi, can anyone help? just want to ask for this DPPH method, is it a must for using a methanol? I mean , we cant do it directly using only the dpph then adding the sample? #stilllearning

@maiaothanh9480

4 жыл бұрын

No, it is must for using methanol or ethanol.

@Rizuk0

4 жыл бұрын

@@maiaothanh9480 currently I'm doing my thesis, and yes, it's a must using either methanol or ethanol

@chemistry_live

3 жыл бұрын

no, not necessary

@STALKER1807

3 жыл бұрын

@@Rizuk0 Hello, im currently on my thesis as well, i might ask you what's the difference between using methanol or ethanol (I know methanol gives better performance during the extraction, but whats the use in here in comparison with ethanol)

@Rizuk0

3 жыл бұрын

@@STALKER1807 Hello ahmed, as for the comparison of both, I'll have to suit it up accordingly to the thesis, when it comes to DPPH assessment for some studies both solvent affects the antioxidants, and these would be one of those consideration ; using one of solvent could affects the DPPH environments for reduction, may some solvent makes the DPPH unstable as it does when reacting with the antioxidants vice versa ; solubility of both solvent should be different, thus affecting the absorbance. Hopefully these answer could useful for you.

where's the paper?

Reference?? Please.

Any other methods?

What's the purpose of using methanol?

@HARHGNK

3 жыл бұрын

organic solvent

I wanted to ask something.....is 58% a good antioxidant value?

@MestebanK

2 жыл бұрын

the question is what was the concentration of the sample you tested... later on you can check what is the parallel concentration of a known molecule (like vitamin e for example)

@amnazahid990

2 жыл бұрын

@@MestebanK my sample was 3ml

Thanks

Reference??

@otabila3830

2 жыл бұрын

Hi, please did you get the reference??, I'm manasseh from Ghana

@zainabalial-qallaf6173

2 жыл бұрын

@@otabila3830 check out Blois (1958) and Yamasaki et al. (1994)