Determination of Starch Content | A Complete Procedure | Acid Hydrolysis Method

Starch constitutes the major component of the polysaccharides in most foods and consists mainly of amylose and amylopectin. The test sample is dispersed in water and heated in acidic solution to hydrolyse the starch, releasing the sugars. The resulting sugars are then determined by titration with Fehling solution according to the method of Lane-Eynon. The sugar content is further converted into Starch content of that sample. This video represents a detailed procedure for the determination of total starch content of a food sample by Acid Hydrolysis Method.
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Пікірлер: 36

  • @JoshiNirav30
    @JoshiNirav30 Жыл бұрын

    Appreciate the amount of work and editing on this video, please make a video on "Total Dietary Fibre by Enzymatric Gravimetric Method"

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Noted. Thanks

  • @hannagraceguirigay1720
    @hannagraceguirigay172010 ай бұрын

    Can you do a video explaining the formula used? Where does the 0.9 come from? 10:09

  • @nurulnadhirah64
    @nurulnadhirah64 Жыл бұрын

    I want to ask, for the solution that needs to be tested for the total sugar method, is it the solution needs to do it immediately, or can wait for a few days?

  • @nurulnadhirah64
    @nurulnadhirah64 Жыл бұрын

    do you have the method to determine starch content by using lane and eynon method?

  • @byrnardsalavedra5969
    @byrnardsalavedra5969 Жыл бұрын

    hi sir/maam, can we use any type of condenser for this analysis? what is most preferred?

  • @alyciaani
    @alyciaani Жыл бұрын

    what gas would be released during the acid hydrolysis please

  • @adepojulanre5234
    @adepojulanre52348 ай бұрын

    Why phenolphthalein indicator not added?

  • @yadavhealthcom
    @yadavhealthcom Жыл бұрын

    Thanks 😊

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Welcome

  • @therese6051
    @therese6051 Жыл бұрын

    Hello! What specific type of reflux condenser is used in this process? Can we use the liebig type of reflux condenser? Or does it have to be the same design and type?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Any reflux condenser what will facilitate the condensation is applicable

  • @vladimirosvasileiadis7742
    @vladimirosvasileiadis7742 Жыл бұрын

    Hello i really need your help. I want to make an acid hydrolysis of proteins. Can you please tell me a method to do this? I know the most common is with hydrochloric acid but i want to learn the method. Thanks

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Digest 2g of your sample with 20ml concentrated Sulfuric Acid

  • @nonatarbah2951
    @nonatarbah2951 Жыл бұрын

    Could you answer me How do you make acides? how calculate them? Is there a way or low for calculate base and acid which use in determine at lab?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Please study chemistry more to do these. Take help from your school teachers.

  • @nainanizami7924
    @nainanizami7924 Жыл бұрын

    can we use this method for determining starch percentage in turmeric?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Yes. You can.

  • @nurulnadhirah64
    @nurulnadhirah64 Жыл бұрын

    so to get the starch content, just only times with 0.9? why 0.9? may i know

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    The reference method said so.

  • @narendarpratapyadav9908

    @narendarpratapyadav9908

    Жыл бұрын

    @@MicroChemsExperiments .92

  • @nurulnadhirah64

    @nurulnadhirah64

    Жыл бұрын

    May i know what references do you use to get 0.9?

  • @FST307
    @FST307 Жыл бұрын

    share vedion on colouring matter determination in ready yo eat food

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Noted

  • @nainanizami7924
    @nainanizami7924 Жыл бұрын

    is this procedure taken from AOAC ?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    No. It is laboratory validated method based on Lane Eynon method

  • @danielpecori6532
    @danielpecori6532 Жыл бұрын

    Hello, can I use this method for determining starch porcentage in corn grains?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Yes

  • @narendarpratapyadav9908
    @narendarpratapyadav9908 Жыл бұрын

    sir starch industry ke bhi video banaye

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Noted

  • @ramisfadhil7384
    @ramisfadhil738410 ай бұрын

    Total sugar 47.5 % how it come? And 0.9 😢

  • @nonatarbah2951
    @nonatarbah2951 Жыл бұрын

    What the meaning 50% Naoh

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Please study chemistry more to do these. Take help from your school teachers.