Very informative ma'am 🙏

Thanks ma'am🙏

Hello I have a question, after ds cut using cas9, how new fragments/ nucleotides are introduced into that cut. Are they introduced in primers of flanking region or through target sequence. Pl. elaborate

@NehasBiology

Жыл бұрын

Actually the DNA breaks are repaired using a method used by cells called NHEJ (Non homologous end joining) where the broken ends are directly ligated without the need of homologous template.

@nitishsharma882

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@@NehasBiology thank you for this information but what about HDR pathway, where we are providing the flanking homologous regions of target sequence.

@NehasBiology

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HDR is also seen in CRISPR-Cas9. And here one strand of homologous DNA duplex results in the formation of hybrid DNA which can then help in DNA repair process.

@nitishsharma882

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@@NehasBiology but the question is how new nucleotides are introduced in HDR?

@NehasBiology

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It is really difficult to explain it here. If you can give me some time, I will make a video on it in 1-2 days.