this is very helpful stuff, thank you

This is exactly what I just did for my midterm practical test!! That’s crazyyy!!

Thank you for the high quality video!

@DavidYazdi1

3 жыл бұрын

Glad we were able to help!

I appreciate this type of content

@DavidYazdi1

3 жыл бұрын

Thanks! -Anthony from Filtrous

Great video! I’m interviewing soon for a position as a cannabis chemist and this is essentially exactly what my job would be. This really helped me visualize everything

@DavidYazdi1

3 жыл бұрын

Glad you found it useful!

@MrJimbob91389

Жыл бұрын

Good luck, do I have to wear a lab coat even if I'm at home?

@timr8431

3 ай бұрын

The sample prep is far from the best method. They're also using way more steps than necessary. Do not follow this video if you are in a cannabis lab.

What HPLC will be good enough to do potency test?

Great video! On the calculations where did you 0.005mg / mL?

@mamenesini

2 жыл бұрын

He’s choosing an arbitrary value as an instrument result. The calibration curve units would be in mg/mL in this case. He’s using this as an example to show the back calculations to reporting units. So obviously we have a 1000x dilution, but our conversion factor is 100: (X mg/mL) x 100 = 5% of that cannabinoid or 50 mg/g in the flower material. In reverse we can assume a 5% by mass cannabinoid will read as 0.05 mg/mL in the instrument. Lastly, this conversion is based on two static variables - 1. The 1000x dilution and 2. The sample weight is always 200 mg +/- a couple mgs. When the sample mass varies you can still use the x100 conversion but you just then multiply by (static weight / actual weight) and you will get your corrected result.

So this isn't something I'm just going to be able to do in the greenhouse. Will look into sending off for testing nearby!

@DavidYazdi1

3 жыл бұрын

Sounds like a great idea!

I understand that it is mandatory to express the concentration in a dry weight basis, how do you take that into account? or is it considered already 'dried'?

You guys should do a GC-FID cannabinoid video. I am extracting CBD CBG and THC for my independent project and this is the instrument I will be performing on. Would you guys prefer HPLC to GC-FID when analyzing these various medicinal compounds?

@AlexPerez-yj8mm

2 жыл бұрын

Independent project? Are you a college student or are you in the industry? Just asking because I'm a graduate student wondering if research is possible in the academic level?

@Homieslice98

2 жыл бұрын

@@AlexPerez-yj8mm I have just recently graduated from NMU’s undergraduate medicinal plant chemistry program which focuses highly on cannabis chemistry. It’s a very new program with lots of potential gearing students for the cannabis industry. I would highly recommend it if you have the ability to learn and apply chemical knowledge in the laboratory. I grew 12 hemp plants for my final project to observe the concentration of CBD at various nodal differences in the plant. What I found was a correlation between the concentrations of cannabidiol aka CBD, is higher when looking at the flowers on the top of the plant. I believe this is due to the light being located near the top plant where energy and nutrients are directed.

@DavidYazdi1

2 жыл бұрын

You'd really want to stick with HPLC for potency. An FID would decarboxylate the acid and you would just get the active form only. For edibles this wouldn't be ideal.

Only delta 9 thc is used .... can we use the sample concentration of thc itself

Great video! Just curious what we’re your conditions for your centrifuge?

@timr8431

3 ай бұрын

Centrifuge is entirely unnecessary in this prep. Idk why they're wasting the time with it.

Would you be able to share an SOP for this?

@DavidYazdi1

2 жыл бұрын

Sorry, this is just a guideline for now. Will look to provide a link to the SOP in the future.

When you purchase the HPLC equipment does it come with the software to analyse the potency? Because i see that there is some software that you are using, does that come with the machine when you purchase it or do you buy the licence for it separate?

@yinfu9301

3 жыл бұрын

Software typically costs extra. I'd talk to the manufacturer to confirm

@DavidYazdi1

2 жыл бұрын

@@yinfu9301 You can buy HPLC equipment from Waters, Shimadzu, Agilent, etc.

Hi, what is the difference if i want to combine HPLC with Mass spectrometry (MS) to determinate the concentration of cannabinoids (CBG, THC, CBD) ? knowing that its a plant culture so i dont' have cannabinoids standards.

@DavidYazdi1

2 жыл бұрын

You need the standards to determine concentration. Also, the concentration of cannabinoids needs to be really low in the sample which will require you to dilute a lot. Usually concentrations are high enough where your standard UV detector would be sufficient.

@BillybatTR

2 жыл бұрын

@@DavidYazdi1 Firstly thank you so much to answer me. If i am undestood correctly, i can find cannabinoids standards on litterature, it will help me to determinate concentration of cannabinoids in my plants. Futhermore, do you have any advice on how many tests should be done to ensure the quality results ? And the fact that its a culture plantation, i have to do test of potency frequently while the plant is growing. So if you have advice on every how many times i have to go in laboratory to do my test... =) Thank you again from France =)

@DavidYazdi1

2 жыл бұрын

How many tests do you think you should do?

Great video not so great weed

This deserve more views ! Very well done ! Except for the shitty weed lmao but it was for the experiment so understandable

@DavidYazdi1

2 жыл бұрын

Not going to waste good weed am I right? LOL

Can anyone explain where sample concentration came from? like which part of analysis result sheet am I supposed to look at?

@ff4064

2 жыл бұрын

I'm talking about 0.05mg/mL.

@DavidYazdi1

2 жыл бұрын

@@ff4064 quoting Sr. Gonkus from another comment: "He’s choosing an arbitrary value as an instrument result. The calibration curve units would be in mg/mL in this case. He’s using this as an example to show the back calculations to reporting units. So obviously we have a 1000x dilution, but our conversion factor is 100: (X mg/mL) x 100 = 5% of that cannabinoid or 50 mg/g in the flower material. In reverse we can assume a 5% by mass cannabinoid will read as 0.05 mg/mL in the instrument. Lastly, this conversion is based on two static variables - 1. The 1000x dilution and 2. The sample weight is always 200 mg +/- a couple mgs. When the sample mass varies you can still use the x100 conversion but you just then multiply by (static weight / actual weight) and you will get your corrected result."

Where did you guys find these methods? Are they AOAC official or?

@DavidYazdi1

2 жыл бұрын

These are just guidelines that are generally used in cannabis labs. Consider it mainly a starting point for your development.

What exact balance are you using? I am guessing 4 sig figs but I am wondering what is the most economical balance you could get away with.

@DavidYazdi1

9 ай бұрын

You typically don't want to go below 4 for HPLC applications. You can always find refurbished units for a good deal!

@Susan-ct6hk

9 ай бұрын

Thanks! If all I’m doing is cannabis potency, what calibration weights do you recommend?

Methanol is not a good polar solvent to extract something polar like THC, try using other solvent for extraction. also use volumetric glassware since this is an assay for potency

@timr8431

3 ай бұрын

Methanol works just fine for extracting THC. Many THC standards are sold dissolved in methanol, so that's just not true.

If someone is available to answer questions about testing edibles I have some

Y'all really be deleting comments with the quickness calling out the poor sample prep, huh?😂

Doesn't the freezing step potentially degrade cannabinoids?

@DavidYazdi1

2 жыл бұрын

Heat would be more detrimental. For the most part if you're just determining THC content it shouldn't affect it. As for cannabinoids, freeze thaw cycles might have an effect, but it's hard to quantify what that affect might be at various temperatures.

@sabinedowner9298

2 жыл бұрын

@@DavidYazdi1 Thanks, I've heard that storing cannabis in the freezer degrades THC to CBN, but that must be over a longer time period.

@Xenmas021

11 ай бұрын

Probably worth running a freeze thaw cycle study for if you're interested :)

U did all that for trash weed lol how much does all that cost 🧐

@DavidYazdi1

2 жыл бұрын

more than your couch

@daveg5847

2 жыл бұрын

@@DavidYazdi1 ummm ok 🤣