Autoclave | Sterilization Process of Glassware and Nutrient Media by Moist Heat Sterilization
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AUTOCLAVE
The autoclave is a container similar to a pressure cooker. It is filled with the objects to be sterilised and then sealed. Next, high-temperature steam is forced in under high pressure, thereby displacing air. Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. The time and temperature to accomplish this depending on pressure and the type of microorganisms to be inactivated. After the necessary time has passed, the steam is released and the sterilised objects are removed. The entire cycle can take from 15 to 60 minutes (batch processing). pushpendra patel solution pharmacy. vandana janghel solution pharmacy. A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker.
PRINCIPLE
The basic principle of steam sterilization, as accomplished in an autoclave, is to expose each item to direct steam contact at the required temperature and pressure for the specified time. Thus, there are four parameters of steam sterilization: steam, pressure, temperature, and time. The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F).
Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. In support of this fact, it has been found that the presence of moisture significantly affects the coagulation temperature of proteins and the temperature at which microorganisms are destroyed.
Uses
Steam sterilizers also are used in healthcare facilities to decontaminate microbiological waste and sharps containers. Autoclave is particularly useful for media containing water that cannot be sterilized by dry heat. It is the method of choice for sterilizing the following:
1. Surgical instruments
2. Culture media
3. Autoclavable plastic containers
4. Plastic tubes and pipette tips
5. Solutions and water
6. Biohazardous waste
7. Glassware (autoclave resistible)
PROCEDURE
1. Place the material to be sterilized inside the pressure chamber and fill the cylinder with sufficient water
2. Close the lid and put on the electrical heater.
3. Adjust the safety valve to the required pressure.
4. After the water boils, allow the steam and air mixture to escape through the discharge tap till all the air has been displaced
5. This can be tested by passing the steam-air mixture liberated from the discharge tap into a pail of water through a connecting rubber tube.
6. When the air bubbles stop coming in the pail, it indicates that all the air has been displaced by steam.
7. Close the discharge tap. The steam pressure rises inside and when it reaches the desired set level (e.g. 15 pounds (lbs) per square inch in most cases), the safety valve opens and excess steam escapes out.
8. Count the holding period from this point of time, which is about 15 minutes in most cases.
9. After the holding period, stop the electrical heater and allow the autoclave to cool until the pressure gauge indicates that the pressure inside is equal to the atmospheric pressure. Open the discharge tap slowly and allow the air to enter the autoclave.
10. Open the lid of the autoclave and remove the sterilized materials.
This video will help you to understand- autoclave working, the principle of an autoclave, sterilization process, the importance of sterilization, sterilization process, moist heat sterilization, working of an autoclave, construction of autoclave, how autoclave works,
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Пікірлер: 275
Very very useful for me...I saw many videos on KZread to understand the autoclave...but no one has made this topic easiy as u did...🥰🥰🥰🥰
@solutionpharmacy
Жыл бұрын
Thank you so much for your kind feedback
Very simple and clearly explained. Thankful for the detail and ease of use
Thank you very much For Your most Valuable video
I like ur video mam.... Aapne bde ache trike se btaya sb
Thankyou ma'am very informative video For what I was looking for 😀😀
Very good exlpanation 👌👌 Thanks so much ..
Mam g Kitna acha smjhaya ha ap ne
Amazing explaination mam 👏👏👏
Thank you for this information 😊
Bahut sahi tarike se bataya maim ne 💯☝️😊👌👌👌👌👌
Very good this is video I understand methods of autoclave👍👍
Thank you mam for explanation....🙏
Easiest video ever to understand working of autoclave tnxxx ma'am:)🥰🥰🥰
Awesome explanation ma'am thanks
Thanks a lot for making this video ma'am
Bahut badhiya explanation thnk you 👍👍
@solutionpharmacy
3 жыл бұрын
Thank you so much
Good explanation with practicals
Bahut bahut thanku mam
Very Good information about Autoclave, I can clearly understanding Thank You So Much😊
Thanks for your information 🙏
Good presentation 👍
Thank you so much ❤️ mam...... nice explanation...👌👌
@solutionpharmacy
4 жыл бұрын
Pleasure
Gr8, THANKS FOR VIDEO
Very helpfull lecture thanks ser ❤❤🙏🙏
Thanku
Best. Video mam.. Thank you❤😍
Thank you. 🙂
Thank you 😊😊 Superb 👍👍👍 This video clear my concept 😊
Good work🤩
We also need to cover the cotton plug (using foil paper, etc) as well, unless it well moist.
@latadonode1051
2 жыл бұрын
Se
@mdmaaz4987
Жыл бұрын
Tujhe bohat mlm hai
Thank you mam very nice explain 👍👍
Thank you for useful information
Very helpful procedure tq u....
Best video on autoclaving
@solutionpharmacy
3 жыл бұрын
Thank you
Very clear and understanding video
@solutionpharmacy
4 жыл бұрын
Thank you
Great information
Thankyou sooo much mam bookage language mai maine kafi baar pdhaaa magar smjh nhi aa raha tha but after watching your video ek hi baar mai procedure smjh mai aa gya 😅
@hannahbaker6105
3 жыл бұрын
Aap kyankrte ho
Excellent Mam
Thnku so much 🤗🤗 it's really very helpful......👍👍👍👍👍
@solutionpharmacy
3 жыл бұрын
Thank you
U explained everything be smoothly
@solutionpharmacy
3 жыл бұрын
Thank you
Thank mam nice video
Thank you mam
Very helpfull video mam🙏
Thanks for sharing the video
Thanku so much
very nice and informative vdo
Excellent mam❤️
Very very simple inform mam thnks
Mam your explanation is very clear..thank you mam
Thanks mom
Thank god for making this video
@solutionpharmacy
3 жыл бұрын
All the best
nyc information..thnkiu
Thank you mam❤️
Very helpful video thq 🎉
@solutionpharmacy
10 ай бұрын
So nice of you
Very clearly explanation...Thank you mam🙏🙏
@haseebjutt9917
Жыл бұрын
Smjh aye ha
Well explained thanku mam
Bahut khub maim
thanx..........nice explanation
@solutionpharmacy
5 жыл бұрын
gopal player Thank you
Thank You
Thnku mam
Thanku very much mam🙏
Good presentation mam
Thanks mam.
Thanks for the video 😊
@solutionpharmacy
2 жыл бұрын
My pleasure
Thanks a lot ma'am
Thank you
Very nice mam
Thank you mam for nicely explain 😊
Appreciate ma'am
Thanks ma'am
Sprrr tqqqq very much
Thanks mam ❤️👍🙏
Very nice video and very helpful thx
@solutionpharmacy
3 жыл бұрын
My pleasure
Thanku you
Helpful👆👍
Nicely explained. Thanks. I need to sterilize a metallic object duly sealed in a pouch. Which pouch I can use for this purpose
@hannahbaker6105
3 жыл бұрын
😂
Thnx mam
Very nice
Thanks for explaining ❤️❤️ can you answer me why we cover baeker and petridish??
@solutionpharmacy
3 жыл бұрын
To prevent from salt depisition
Thank u mem🙏
Nice information 👍
@solutionpharmacy
2 жыл бұрын
Thank you
Pls tell me how to maintain 15psi temp?
tq so much
mam 15 lbs k upar pressure jane k baad whistle apne aap bajne lagta hai ya use manual karna padta hai please reply
Fantastic 🎉
@solutionpharmacy
6 ай бұрын
Thanks 🤗
Mam thanks
Thanks
@solutionpharmacy
2 жыл бұрын
My pleasure
Awesome
Good translation mam.
@solutionpharmacy
3 жыл бұрын
Thank you
Thankyou mam
Thank u mam
MAM STERILISED SURGICAL DRUMS KE INSTRUMENTS (AUTOCLAVED) KO HUMLOG KITNE DINO BAD TAK PRAYOG ME LA SAKTE HAIN.
Thank you so much mam
@solutionpharmacy
3 жыл бұрын
My pleasure
Mem hitting joint may lik hoya kiya souliotion hai? Same autoclave.
Tq
Please help me incubator par vedio bna dijiye
tnqq very useful for us🙏🙏
Tq man😘😘
Flask ko bhi cotton ke upper paper lgate hai
So nice vedio
@solutionpharmacy
5 жыл бұрын
Thank you