You’re a life saver. Thank you. This was so helpful.

best and cleariest and absolute explanation ever, thank you so much! :D

Very helpful, needed this refresher. Much Thanks! :)

Thank you , this is very helpful !

This makes sense! good explanation.

Helpful video. U tell me meaning of spike.... thank u very much

Thank you very much. It is very helpful

thank you!

Thank youuu!!

i found more area of analyte than standard in egg matrix ( LC-UV analysis) sample 5 g reconstitute volume 2ml spiked conc 1 ppm how do i calculate conc of analyte to find out recovery? area of sample 57289 area of standard 42380

Why is it multiplied by 10?

@MAngelier

2 жыл бұрын

Because each time he added 10mL of the sample in a total volume of 100mL, so it gets 10 times diluted. The measured value is therefore actually 10x to low, to compensate for this, multiply by 10 in the end.

Hi, just wanna ask. Why do we need to multiply 10 to the calculated concentration? (As per the example calculation)

@ChemEngTutor

Жыл бұрын

We need to multiply by 10 because the initial specimen solution was diluted by a factor of ten when the standards were made.

Thank you. It was very useful. How can we find the percentage recovery?

@ChemEngTutor

Жыл бұрын

To do this, you need to know the concentration of analyte you were expecting to get. You can then work out the experimentally determined concentration as a percentage of the expected concentration. I hope this helps.

Why do we extrapolate it to when the instrument response is zero? Doesn't a zero response mean the absence of analyte?

@ChemEngTutor

Жыл бұрын

We are trying to get the difference between when there is zero analyte (y = 0) and when we just at have the instrument response for the specimen (x = 0). I hope this helps.

from where 0.506 ???

@funnysundayfunnys8537

2 жыл бұрын

Use y=mx+c formula, 0.506 in this case is the gradient/slope of the sample.

@matthulvey8615

Жыл бұрын

All values of the calibration plot are from least squares regression which finds the algebraic formula that best fits the raw data. The constants are merely the slope and intercept with x and y as your variables. Typically you would use software to calculate, but you can do so manually.

@ericdominic8382

Жыл бұрын

Wondering too