Thank you!!!! our Microbiology teacher is really lazy, she teaches us nothing! Its really good that I can watch videos like yours and learn what I need! :)

Thank you so much. You’ve helped me for my exam tomorrow. Wish I saw this sooner, but you taught it to me so quickly and I actually understand.

Wow, you are good! I love how easy it is to understand the information. Thank you so much

You explained this so well! Thank you so much for your help!

came to watch your video to review my Microbio... got an exam tomorrow and I can't just read all about AFS... your's is so helpful... *thumbs up* Thanks! :)

@SnehaMunni

6 жыл бұрын

M I S S talker kzread.info/dash/bejne/d6eDtaOxlKraaLA.html

Thank you so much!! The visual and explanations definitely help!! I will be looking at your other videos as well to be clear on the other subjects in regards to staining. Thank you thank you thank you!!!

@BiologyProfessor

6 жыл бұрын

That's great to hear that my videos are helping you! :) Check out this playlist for all of my videos on different stains: kzread.info/head/PLjMo-ys5HQIY2ZL8FDSoabCy4gH9D9rbd

You are an amazing teacher, thank you!

You've explained it so well 👏👏👏👏 Glad I came here

Thank you very much. Deeply Appreciated. I recommend this video who wants to learn about Acidfast

If you were my professor I'd come to class early and leave late!! Thanks for vid love:)

@likeaphoenix6

2 жыл бұрын

Why

Your videos are so helpful! Thank you.

thank you!! i've been getting really mixed up with this stain, this was nice and simple to learn!

@BiologyProfessor

5 жыл бұрын

I'm glad to help! I also have videos on the Gram Stain, Flagella Stain, Endospore Stain, Capsule Stain and more if you want to check them out! Here is a link to a playlist on all my videos relating to Microbiology Stains: kzread.info/head/PLjMo-ys5HQIY2ZL8FDSoabCy4gH9D9rbd

Thanks so much I really learned a lot from your videos please continue to make more and keep up the good work.!!!

U makes everything simple and helpful...thankq..

Thanks for share the Z N stain procedure.

You are an amazing teacher , watching your videos to pass my Mbbs exams

Amazing video and excellent explanation Thank You Great job

Thats was a great explanation. Thank you. You rock!

Amazing explanation, bravo!!!

This is lovely and very helpful...appreciate

Thank you very much. This is a great video.

Very nicely narrated !! Thanks a lot 😁

you know am very busy learning medical microbiology...but i just had to sign in to like your video. Thank you, you are a great teacher! keep at it

@SnehaMunni

6 жыл бұрын

EDUARDO12348 kzread.info/dash/bejne/d6eDtaOxlKraaLA.html

This is exactly what I needed!

@BiologyProfessor

3 жыл бұрын

Great! :)

Explained so good

thanks for your video , but i think you missed mention about heating when you add strong carbolfuchsin , it is crucial step in AFB stain

Fantastic. Thank you!

THANK-YOU. YOU MADE IT SO EASY.

Amazing , now if my professor could just explain it this way! They make it sooo much more confusing. Thank you!!

You are a life saver

Can you do a video on Counting CFUs? Determining Bacterial numbers. Thanks for these informative and well though-out videos.

Super helpful, thank you!

Great video!

thank u this helped me a lot!

Awesome teaching.....

thanks alot, idid not seen like you.

great explanation 👏

Excellent video ❤

Thank you!

Thank you.... So much

Super helpful thank you

Thank you

please make more videos, you did a great job!

@SnehaMunni

6 жыл бұрын

Akbar Hamdard kzread.info/dash/bejne/d6eDtaOxlKraaLA.html

thanks for this, rlly helped

This is awesome, thank you!

@BiologyProfessor

2 жыл бұрын

Glad you like it!

Thank you!!!! This helped out a lot!!!

@BiologyProfessor

2 жыл бұрын

I’m glad 😀

Thanks!

huge thank you 🙏🏼🙏🏼🙏🏼🙏🏼🙏🏼💙💙💙

Amazingly explained 💯👏😘.

@BiologyProfessor

2 жыл бұрын

Thanks a lot 😊 - please subscribe and tell your classmates about this channel for studying. :)

thank you very much

Wow thank you

It's awesome. tks

Thank you sooo much

thnx for sharing this. thumbs up

Thanks

THANK U!

Awesome thanks

Thanks finally i understand it

thanks

god bless you!

So much helpful!

@BiologyProfessor

6 жыл бұрын

I'm glad! Thanks for watching! If it would be helpful, check out my playlist of videos on different kinds of stains, like the Gram Stain, Endospore Stain, and Flagella Stain: kzread.info/dash/bejne/a6Gky9h_p6fMc5s.html

Nice thanks

THANKS!!!!!

Thank u so much

Thnks

Good job professor 👏🏼👏🏼👏🏼👏🏼💜

@BiologyProfessor

2 жыл бұрын

Thanks :)

2022! Still a very great video for study this

@BiologyProfessor

2 жыл бұрын

Yes, it has aged well. ;)

Thank you it is helpfull for me

@SnehaMunni

6 жыл бұрын

B R kzread.info/dash/bejne/d6eDtaOxlKraaLA.html

Thank u madam... Usefull video😁

perfect!!!!!

SHE IS SO FREAAKING GOOD.LOTS OF LOVE I WISH YOU WERE MY BIOLOGY PROFESSOR

Great

I like your vidoes

Wonderful explanation! Thank you so much :)

Also, could you please make a video on CFU? Thanks again :)

@BiologyProfessor

7 жыл бұрын

I'll add it to my list, but it might be a few weeks. Thanks for the suggestion!

thank you :)

@BiologyProfessor

2 жыл бұрын

You're welcome!

Micro blb bg zn afb stainings not ditctiva ka Matlab Kya hei

I would recommend that you make another channel called Microbiology Professor, or just include that word in the title.

Thanks 🇸🇦♥️♥️

Thank you so much. It's important to emphasize that the Acid-fast stain is Ziehl-Nielsen's stain so that beginners in Microbiology don't get confused.

@231mac

4 жыл бұрын

Yeah... that was a kinda dumb request, lol.

You are awesome. :)

SPECIAL STAINS* - Lipid accumulations*- Oil red O stain Sudan black B or IV Osmium tetroxide Glycogen* -Best carmine PAS Melanin* -Fontana masson staining Hemosiderin* -Perl's prussian blue stain Calcium* -Von kossa* Alizarin red Calcein stain Tetracycline labelling index*

perfect

@BiologyProfessor

2 жыл бұрын

thanks :)

so during an acid fast stain what color would the gram negative stain if a non acid alcohol were to be used as a decolorizer? clear?

@BiologyProfessor

2 жыл бұрын

probably red - without acid in the decolorizer, the carbol fuchsin would not leave the cells.

leprae is a gram positive so how can you use acid-fast stain to detect it?

@BiologyProfessor

7 жыл бұрын

Based on its cell wall structure, Mycobacterium leprae is a gram-positive, rod-shaped bacteria with a thick waxy coating. Due to this coating with mycolic acid, it cannot be stained very well with the regular Gram stain procedure. That is why we use the acid fast stain on it instead.

I think you should go back and redo some of these videos as I feel it's important to mention what the mordant is for example.

Love it thank you could you please do like this video with all stains🥺❤️

@BiologyProfessor

3 жыл бұрын

What stains do you need? Here’s my playlist to all my stain videos: kzread.info/head/PLjMo-ys5HQIaQWhxrInBxjMjuJOsNhqSz I’ve got: Gram Stain: kzread.info/dash/bejne/Y4d9tqWrl6mZqc4.html Acid Fast Stain: kzread.info/dash/bejne/hnd60rdxoJaoqZc.html Endospore Stain: kzread.info/dash/bejne/a6Gky9h_p6fMc5s.html Flagella Stain: kzread.info/dash/bejne/pp6VubN-fKXbYbA.html Capsule Stain: kzread.info/dash/bejne/kZiKxZhultrVg9Y.html Acidic and Basic Stains: kzread.info/dash/bejne/ont4pKOIZtKvfrw.html

👍

Loved ❤️ from Islamic Republic of Pakistan 🇵🇰. May Almighty Allah bless you sister

@BiologyProfessor

2 жыл бұрын

Thank you.

hello prof. if the bacteria is non acid fast then it is gram negative?

@BiologyProfessor

7 жыл бұрын

Not necessarily. You would need to actually do a Gram stain. Non acid fast bacteria can be Gram positive or Gram negative. The acid fast stain just shows that non acid fast bacteria don't have mycolic acids, but it cannot assign their Gram type.

What outcome (color) is expected for a Gram positive, non-acid fast bacterium like Staphylococcus aureus if alcohol is mistakenly used instead of acid alcohol?

@BiologyProfessor

10 жыл бұрын

Alcohol is not good enough to remove the carbolfuchsin dye from non-acid fast bacteria because carbolfuchsin isn't very soluble in it. So using alcohol instead of acid alcohol will not decolorize properly and more non-acid fast bacteria will appear red. Hope this helps!

@berniedmjm4108

10 жыл бұрын

Awesome!!! Thanks!!!

hi . i have question. why do some lab use malachite green as a secondary stain ???

@BiologyProfessor

4 жыл бұрын

Personal preference, I guess.

@231mac

4 жыл бұрын

Our lab did whenever we ran out of Methylene blue, lol. I think under certain microscopes Malachite green showed up better.

Why is acid alcohol used as a decolorizer in an acid fast stain, as opposed to a neutral alcohol solution?

@BiologyProfessor

7 жыл бұрын

because carbolfuchsin is a basic dye that is soluble in acidic solutions (like acid alcohol or the mycolic acids in the acid-fast cell walls)

@epheriaplace3002

7 жыл бұрын

thanks for reply Prof. So whats advantage of alcohol? pls

Super mam useful video

@BiologyProfessor

3 жыл бұрын

Thanks! Tell your classmates 😉

@vigneshsfc9404

3 жыл бұрын

@@BiologyProfessor yeah sure

question: i was working on an unknown bacteria and i was doing this procedure to identify my organism. I had a positive control on one end of the slide and my unknown on the other end; when i performed this procedure my control positive also came out blue! why!? my unknown bacteria also came out blue; so the control was compromised and i had to start over.

@BiologyProfessor

7 жыл бұрын

Maybe you used too much decolorizer or left it on for too long? Here is a link to some other troubleshooting tips: faculty.lacitycollege.edu/hicksdr/staining.htm

@savitakadian6318

7 жыл бұрын

Allan Ochoa

what are the reasons for false positive result?

@BiologyProfessor

7 жыл бұрын

Several things can result in a false-positive, including cross-contamination between samples in the lab, contamination of diagnostic equipment, and improper use of the staining protocol, like not treating the smear with decolorizer like enough.

Teacher you didn’t mention ,about the heat part which acts as a mordant 😢😢

What result do we get if we stain the acid fast bacteria with Grams staining??

@BiologyProfessor

7 жыл бұрын

They are technically gram-positive, but I don't know that they stain well with the Gram stain because of the mycolic acids in their cell wall.

@SnehaMunni

6 жыл бұрын

Mamta Tamang kzread.info/dash/bejne/d6eDtaOxlKraaLA.html

Why used acid fast staining for actinomycetes

@BiologyProfessor

2 жыл бұрын

To distinguish it from Nocardia. Actinomyces and Nocardia can be difficult to distinguish without staining. Acid-fast Nocardia can be distinguished from from non-acid-fast Actinomyces with an acid fast stain.

Why is ZN stain used instead of Gram stain in spite of the organism being a bacteria?

@BiologyProfessor

3 жыл бұрын

Acid-fast bacteria don’t stain well with Gram stain because of the waxy mycolic acids in their cell wall. So another stain is necessary.